Skip Navigation LinksHome > June 2001 - Volume 12 - Issue 4 > Stability of coagulation proteins in frozen plasma
Blood Coagulation & Fibrinolysis:
Original Article

Stability of coagulation proteins in frozen plasma

Woodhams, B.; Girardot, O.; Blanco, M. -J.; Colesse, G.; Gourmelin, Y.

Collapse Box


This study reports on the frozen stability of all commonly measured coagulation proteins in normal citrated plasma: activated partial thromboplastin time, prothrombin time (%), thrombin time and fibrinogen (Clauss); clotting assays for factors II, V, VII, VIII, IX, X, XI and XII; functional assays for protein C (clotting), protein S (clotting), antithrombin (chromogenic) and plasminogen (chromogenic); and immunological assays for von Willebrand factor and D-dimer. All these factors listed are stable for up to 3 months if frozen at −24°C or lower. At −74°C, all these factors (allowing for 10% variation) were stable for at least 18 months, most were stable for 24 months. The number of proteins showing > 5% variation over baseline after 6 months storage indicates that some decay does occur even at −74°C. There was no clear advantage in snap freezing at −74°C and then storing at −24°C over both freezing and storing at −24°C; therefore, the freezing process itself is not responsible for the loss of stability. The best stability, especially at −24°C, was obtained when small samples (1 ml) were stored in screw-cap tubes with a minimum dead space. The decrease in stability of the coagulation proteins directly correlates with the effect of temperature and time.

© 2001 Lippincott Williams & Wilkins, Inc.


Article Tools


Article Level Metrics

Search for Similar Articles
You may search for similar articles that contain these same keywords or you may modify the keyword list to augment your search.