Infection is a common problem in chronic wounds. It is one of the key reasons why wound healing may stall, leading to increased risks of patient morbidity and mortality. The purpose of this article is to review the assessment and practical management of infection in a chronic wound.
Before we can manage infection, we must first understand the complexities of the microbial-host environment.1 Bacteria seek to establish themselves in ecological niches to ensure their own survival and evolution. An open wound is a suitable niche. The longer a wound is open, the more inviting it is for bacteria. Host resistance is the single most important determinant in what happens to those bacteria and ultimately, the outcome of infection in a wound. All chronic wounds become "contaminated" and "colonized" with bacteria.
Skin is the first line of defense as a physical barrier against microbial invasion. In the normal setting, low surface pH, sebaceous fluid, and fatty acids inhibit the colonization and growth of pathogenic organisms (Figure 1).2
DEFINITIONS OF IMPORTANT WOUND TERMINOLOGY
* Contamination refers to the simple existence of bacteria within a wound. All chronic wounds are contaminated. Bacteria are low in number and non-replicating. The host is "in control" and there is no evidence of bacterial-induced damage to the wound. The wound may continue to heal.
* Colonization is the next step in the evolving relationship of microbes with the host. Bacteria are now replicating, and a larger population is now established in the wound. The host remains in control. There is no evidence of tissue invasion, and the wound will heal. In most cases, wound colonization is polymicrobial.3 Wound colonizers usually originate from 3 potential sources:
1. Surrounding skin, including local skin organisms such as Corynebacteria spp, Propionibacteria spp, coagulase-negative Staphylococci, and viridans streptococci
2. External environment, including multi-resistant organisms (MROs), such as methicillin-resistant Staphylococcus aureus (MRSA)
3. Endogenous sources, usually involving mucous membranes: oropharyngeal, gastrointestinal ("fecal veneer") or genitourinary mucosae, including a range of microorganisms such as Streptococcus spp, coliforms, and anaerobes
* Critical colonization is that crucial step where the confrontation between bacteria and the host creates the first fissure to develop within the protective ranks of the host, usually within the superficial compartment of the wound.
* Infection is defined at the point where bacteria begin to invade deep compartments and break through the layers of host defenses to damage tissue. A critical density or burden of bacteria has been reached and wound healing becomes stalled or reversed. Typically damage involves dermal or sub-dermal tissue. Bacteria may then in turn gain access to the systemic circulation.
The probability (P) of infection varies directly with increasing bacterial numbers and their relative ability to cause disease, known as virulence, while varying inversely with the host's ability to resist invasion, expressed in the following formula:
Historically, a deep tissue quantitative microbial count of >105 CFU/mL has been associated with a higher incidence of wound sepsis4; however, the dose of "infecting" bacteria varies depending upon the specific microorganism. For example, it is lower for β-hemolytic streptococci and Pseudomonas aeruginosa and higher for Enterococcus, diphtheroids, or fungi. Put another way, Bendy5 claimed that wound healing in decubitus ulcers progressed normally only when the microbial load was <106 CFU/mL of wound fluid.
Practically, we do not perform routine quantitative wound biopsies in clinical practice, because of factors such as cost, time, potential sampling error, and risk of introducing infection, but semi-quantitative surface wound swabs correlate well with deep tissue quantitative counts.
POLYMICROBIAL NATURE OF CHRONIC WOUNDS
Chronic wounds have a complex microbiological environment with a mixed flora, which changes over time. Coagulase-negative staphylococci, Streptococcus spp, Corynebacterium spp, and S aureus populate the wound initially before facultative anaerobic Gram-negative bacilli, such as E coli, Klebsiellae, or Proteus spp take up residence, usually days to weeks later. The longer an ulcer remains unhealed, the more likely it will acquire multiple aerobic organisms (mean 4.3 species), as well as a significant anaerobic population (mean 2.0 species).3 Chronic wounds have a statistically higher proportion of anaerobes as compared to acute wounds (2.0 species vs 1.1, respectively, P = .05). Anaerobes are not identified on routine microbial culture swabs because of specific isolation requirements, and their presence is usually under-appreciated by the treating health care worker. Frequent anaerobic colonizers include Prevotella, Bacteroides, Peptostreptococcus, and Porphyromonas.6 More than 95% of diabetic foot infections contain anaerobes along with aerobes, such as S aureus, Enterococcus, and coliforms.7 Aerobic Gram-negative bacilli, such as Pseudomonas and Acinetobacter spp come from exogenous sources, such as foot or bath water. Pseudomonads are not highly invasive unless the patient is immunocompromised, and in this situation, their microbial exotoxins and endotoxin exacerbate tissue damage.
Some bacteria work together in microbial synergy.8 Their net pathogenic effect is greater than if these same organisms worked independently of each other. In mixed aerobic/ anaerobic infections, microbial synergy frequently exists. The effect of synergy between 2 bacteria can be devastating for the host, especially if the synergy fosters a rapidly destructive necrotizing fasciitis (Bacteroides spp, group A streptococci, Peptostreptococcus spp). Less invasive microorganisms like coliforms can be synergistic with more virulent ones and play a crucial role in wound infection. For example, microorganisms like Klebsiella can promote the growth of Prevotella spp by providing key growth factors such as succinate. S aureus can also promote the growth of anaerobes through the provision of growth factors. Synergy is common among Bacteroides spp, aerobic or anaerobic bacteria, Peptostreptococcus spp and P aeruginosa or S aureus.9 All of these are common pathogens found in the beds of diabetic, vascular, and pressure ulcers.
In deep tissue infection involving fascial planes or bone, both aerobes and anaerobes are active, and are present in higher numbers (mean total 5.8 species, with mean 2.3 anaerobic species). Anaerobes grow well in the presence of low oxygen concentration. Such chronic wounds frequently have low tissue pO2 levels, which can range from 5 to 20 mm Hg.10 For wounds to heal, tissue pO2 levels should be a minimum of 30 mm Hg.
Most wound anaerobes are facultative or microaerophilic, ie, tolerating some oxygen. However, the presence of cell death caused by tissue hypoxia creates ideal growth conditions for wound microflora. Fastidious or strict anaerobes, such as Bacteroides fragilis, proliferate as residual tissue oxygen is consumed by facultative bacteria. In suitable numbers, these anaerobes can express adhesion factors, destructive exoenzymes, and antiphagocytic factors, all of which contribute to poor wound healing. The lack of local oxygen inhibits the oxidative burst activity in polymorphonuclear leukocytes that generates the intracellular production of antimicrobial metabolites,11 while reduced leukocytic killing capacity exists if tissue pO2 <30 mm Hg. Consequently, the inflammatory steps of successful healing fail to occur. These are the conditions frequently found in relatively avascular wounds, such as those seen in deep diabetic, arterial, or pressure ulcers.
Chronic venous leg ulcers also have a complex polymicrobial aerobic-anaerobic flora. Half of the microbial flora of these infected ulcers is anaerobes.9 Decubitus ulcers, particularly over the trochanteric and sacral areas, will experience some fecal contamination, which contains high numbers of anaerobes. The potential for microbial synergy in this setting is high, and wound deterioration may reflect cooperating microorganisms.12 Deep decubitus ulcers are at high risk for developing underlying mixed aerobic/anaerobic osteomyelitis and bacteremia, and can be an unrecognized source of fever in a debilitated patient. In these settings, debridement of necrotic tissue is the key to reducing the risks of worsening infection, as is effective broad-spectrum antimicrobial therapy.
The presence of microorganisms, such as Enterococcus, Candida spp, or MRSA, does not always indicate infection, if they are present in low numbers. Treatment is not routinely indicated. However, some bacteria are always significant, particularly group A beta-hemolytic streptococci, Mycobacteria, and Clostridium spp. An acute exacerbation of a chronic wound with cellulitis or erysipelas frequently involves streptococcal or staphylococcal species. Rare and unusual secondary infections of chronic wounds may include: Erysipelothrix rhusiopathiae seen in raw meat or fish handlers, and mycobacterium marinum/ulcerans acquired from aquaria, pools, or water.
CHOOSING THE RIGHT ANTIMICROBIAL AGENT FOR A CHRONIC WOUND: DISINFECTANTS, ANTISEPTICS, OR ANTIBIOTICS?
The use of antimicrobial agents in wound care is largely empirical and is focused upon preventing or treating critical colonization and infection. Recommendations for making sound antimicrobial choices are made primarily on expert opinion. One must recognize what to look for in terms of infection. This is a clinical bedside skill in spite of much debate attempting to correlate specific levels of bacterial burden with the development of infection. Table 1 summarizes ways to approach the assessment of infection at the bedside.13 If clinicians conclude that critical colonization is present, then it is reasonable to adopt a topical therapeutic approach using antiseptics or topical antibiotics. However, if deeper compartment involvement is suspected, then a systemic antibiotic approach should be made, using oral or parenteral antibiotics.
The term "antimicrobial agents" comprises: disinfectants, antiseptics, and antibiotics. It is useful to differentiate these terms as their usages have significant implications on wound healing.
Disinfectants are chemicals that kill microorganisms on any surface and are usually harmful to human tissue. These are not appropriate for managing wound infections.
Antiseptics are agents that inhibit growth and development of most microorganisms in or on living tissue. Compared to antibiotics, antiseptics are broad spectrum and generate relatively little antimicrobial resistance. They are used selectively for short periods of time to reduce bacterial burden, but may have adverse effects on healing tissues. Although in-vitro experiments suggest that antiseptics are cytotoxic to fibroblasts, leukocytes, and keratinocytes, it is not clear that in vivo effects are similar, as small studies have shown that some wounds may continue to heal in spite of the use of antiseptics like povidone-iodine. A list of some common antiseptics is provided in Table 2.
Povidone-iodine (PVI) is a combination of antibacterial molecular iodine and polyvinyl/pyrrolidone. It is available in several forms (solution, cream, ointment, scrub). For the purposes of wound care, the solution is used as a range of 1% to 10% on wound surfaces. Numerous studies have been conducted to determine the safety and efficacy of iodine compounds on bacterial control and wound healing. Few studies have been done in chronic wounds, while animal models do not really approximate a chronic human wound situation.14 Although animal studies have not confirmed the efficacy of PVI solution in terms of reduction in bacterial counts 12 hours after treatment, some human trials have shown reductions in clinical wound infections.15 Expert opinion would suggest using PVI for chronic nonhealable wounds to facilitate bacterial reduction to stabilize the wound. In a pilot study to assess the effectiveness of PVI as an antimicrobial agent to manage maintenance and non-healable wounds, Woo et al16 demonstrated an overall 28% complete closure rate and a 45% reduction in wound size in 42 patients with diabetic foot ulcers using 10% PVI.
Some concern exists that PVI is anti-mitotic in vitro and may adversely affect tissue repair.17 The question of the effect of PVI on overall wound healing is more difficult to answer because of limited comparability of studies, which vary in wound healing definitions, assessment times, and control groups. In burns, PVI may have some concentration-dependent cytotoxicity, but in general, PVI does not have any specific independent effects on chronic wound healing, apart from possibly limiting wound sepsis and thereby facilitating the natural healing process. There is virtually no antimicrobial resistance to PVI.18
Cadexomer iodine consists of spherical hydrophilic beads of cadexomer-starch, which contain iodine, available as an ointment or dressing. It is highly absorbent and releases iodine slowly into the superficial wound compartment. Cadexomer iodine has been shown in a small randomized trial to accelerate the healing rate of decubitus ulcers. This may be because of its enhanced ability to absorb wound exudate, thereby removing inhibitory cytokines and matrix metalloproteases. Antibacterial iodine is in turn released into the wound.19 Similar results have been shown in venous ulcers, while there was a corresponding decrease in infection rates.20-22 Daily applications of cadexomer iodine have also been shown to reduce levels of MRSA and other wound bacteria in comparison to placebo controls over a 3-day period in a pig model.23 Expert opinion would favor using cadexomer iodine for short-term use in healable wounds where bacterial burden is high in the superficial compartment.
Even though hydrogen peroxide still has a popular following in some circles, it has no significant influence on wound healing, and is ineffective in reducing bacterial wound counts. Although virtually no studies have been done in chronic wounds, hydrogen peroxide may be useful as a chemical debriding agent to loosen necrotic tissue and other wound debris during its effervescent phase.
Hippocrates in the 5th century BC used vinegar or acetic acid as a wound antiseptic. As a 0.25% to 0.5% solution, it is bactericidal against many Gram-positive and Gram-negative organisms, and is effective in reducing bacterial burden. By convention, it is a popular adjunctive short-term treatment for superficial wound infections with Pseudomonas aeruginosa. Since Pseudomonas spp typically develop quick resistance to many topical and systemic agents, this simple approach, which reduces local pH, can reduce the bacterial burden of this microorganism in the wound. Diluted vinegar soaks for 15 minutes per day are effective and reduce problems of local wound odor if a mixed aerobic-anaerobic flora is present. This approach is effective for critically colonized wounds in the superficial compartment. In a venous leg ulcer study, gauze dressings wetted with acetic acid decreased the number of S aureus isolates and Gram-negative rods.24 Although some in-vitro studies have suggested that acetic acid is cytotoxic, these findings have not borne out in the in-vivo arena using conventional treatment doses.
Chlorhexidine has wide use in medical practice as an antiseptic with broad-spectrum antimicrobial activity. It is safe as a surgical irrigation solution and is best used at a concentration of 0.02%. Although it has no apparent independent effect on wound healing,25 it may favor improved healing times by limiting wound infection. Human studies are otherwise limited to clearly answer this question.
Although silver has been used for medicinal purposes and water purification since ancient Greece, its use in burns in the modern era has extended its application into chronic wound care. Silver has broad antimicrobial properties, particularly against MRSA and vancomycin-resistant enterococci (VRE), as well as anti-inflammatory activity. It has several mechanisms of action including: blocking nutrient transport through bacterial cell walls; denaturing proteins involved in microbial respiration; and binding to microbial DNA, inactivating protein translation and replication of DNA.
Silver sulfadiazine is still used in topical formulation as a cream primarily in burns, whereas silver-releasing systems have been developed for increased efficacy and reduced toxicity in chronic wound management, as well as burns. Silver must be presented in ionic or nanocrystalline form to exert an antimicrobial effect. It requires a fluid phase with intimate proximity to the wound for the silver to access the superficial compartment. Its primary activity is within the superficial wound compartment with very limited activity within the deep compartment. The advantages of the newer silver preparations include less systemic absorption; combining the antimicrobial effects of silver with dressings that address moisture balance and autolytic debridement; and no dermal deposition of silver (argyria). The best examples are described in Table 3.
Sibbald et al26 used nanocrystalline silver (Acticoat, Smith & Nephew, Largo, Florida) to assess 29 stalled chronic wounds, and showed improved superficial compartment critical colonization on quantitative biopsy. In a small pilot study, using Acticoat-7 in stalled venous ulcers applied at weekly intervals, Sibbald et al27 demonstrated a reduction in S aureus counts and enhanced healing in one-third of the patients at 9 weeks.
In an international randomized controlled trial (RCT) comparing a medium-Ag release foam (Contreet, Coloplast, Minneapolis, Minnesota) with foam dressing alone without Ag (Allevyn, Smith & Nephew) in patients with venous disease, there was a 45% median relative reduction in wound size at 4 weeks with the Ag-containing foam, compared to 25% relative reduction in wound size with the standard foam (P = .03). Exudate management and odor control favored the silver dressing. Comparing the use of Contreet to local best practice in a similar population, relative reduction in wound size of 50% favored the silver dressing.
Interestingly, in a 2007 Cochrane review, 3 RCTs were found containing 847 patients. These 3 trials compared a silver-containing alginate (Silvercel, Johnson & Johnson, Somerville, New Jersey) with alginate alone (Algosteril, Smith & Nephew). Although wound size reduction was noted using the Ag-containing foam at 4 weeks, no differences were noted in enhanced complete wound healing. No recommendation was made regarding Ag-containing dressings because of insufficient evidence and short observational time lines.
Decisions for choosing an antibiotic are made on the assessment by the clinician as to whether the wound is thought to be critically colonized or infected. Although superficial compartment infections may be treated with topical agents, infections of the deep compartment require systemic antibiotics.
Antibiotics are drugs, when given topically or systemically, that inhibit the growth of microorganisms. About a quarter of all persons with chronic wounds are receiving antibiotics at any one time. Approximately 60% have received systemic antibiotics within a previous 6-month period.1 There is no evidence that prophylactic or routine use of antibiotics in chronic wounds has any role in the absence of clinical infection or increased bacterial burden. Expert opinion guides the use of topical antibiotics, but clear indications and durations of usage in a wound are unclear. Although topical antibiotics, such as silver sulfadiazine, may reduce the bacterial burden in critically colonized wounds, the down-side of usage includes host sensitization, contact dermatitis, and promotion of antimicrobial resistance. Most studies of topical antibiotics in this field are difficult to compare and few have sufficient power to draw meaningful conclusions. Although mupirocin has been shown to be successful in the topical treatment of acute impetigo, and topical silver sulfadiazine has been used in burn management, these studies do not include chronic wounds, so the generalization of results is limited.
Consequently, our initial antibiotic treatment of infected chronic wounds rests upon an empirical approach (Table 4), usually because of an absence of specific microbiological data at this point. Unfortunately, the results of culture material from a wound may not give the whole microbiological picture. The most meticulous culture techniques may give only a range of microbial isolates, but not tell us exactly which organism is the infecting culprit. It is our knowledge of what isolates are likely to be present and what they can do, as well as the health status of the host, that helps us decide upon the best practical treatment plan.
We do not have an in-vivo laboratory model of an infected chronic wound to test treatment hypotheses. In general, the main indications for starting topical antibiotics are for superficial compartment infections or critical colonization and where the clinical scenario fits the NERDS mnemonic (Table 1). Whether one chooses to start a topical antibiotic, local antiseptic agent, or a silver-containing dressing at this point remains a clinical decision based on expert opinion. A systemic antimicrobial approach is taken where the scenario fits the STONEES (Table 1) mnemonic. Clearly, the decision to start antibiotics and what to choose initially is only as good as the acumen of the bedside clinician.
Foot ulcers such as those associated with diabetic or vascular disease require a high bedside index of suspicion for infection as the consequences of limb- or life-threatening infection can be high. With a lower clinical threshold to begin antibiotics, broad-spectrum agents are started to cover the polymicrobial flora involved. Karchmer and Gibbons28 questioned the necessity of precisely defining the causative bacteria. They suggested that the treatment of these infections could be based on a clear understanding of the wound microbiology.28 Armstrong29 agreed by concluding that repetitive cultures following initial culture and subsequent treatment do not confirm or exclude the presence of infection. Therefore, foot infections must be diagnosed and treated on clinical grounds.
The general rule of thumb in choosing antibiotics is to determine whether infection is likely to be present, and then to decide whether that infection is likely to be mixed aerobic/anaerobic. In most cases, broad-spectrum treatment will constitute the initial regimen.
With increasing frequency, chronic wounds are seen colonized and/or infected with MROs, such as MRSA. Infected wounds, which do not respond to treatment, should be evaluated for the presence of an MRO. Risk factors that increase the likelihood of MRSA include recent hospitalization, transfer from a chronic facility, and previous antibiotic use. An increasing number of wounds are affected by community-associated MRSA, where no identifiable risk factors are noted.30
A wound colonized with MRSA requires infection prevention and meticulous control measures to prevent spread to other patients. Consistent hand hygiene on the part of managing health care professionals is important in reducing spread.31 Health care facilities all have protocols to reduce such spread.
A patient colonized/infected with MRSA frequently carries the organism at multiple body sites (ie, nose, rectum, axillae, and perineum). Colonized wounds should be followed carefully for the development of infection. However, topical and systemic antibiotics should be avoided if clinical signs of infection are absent. Indiscriminate use of topical agents like mupirocin, fusidic acid, or clindamycin increases the likelihood of MRSA developing high-level antimicrobial resistance.32 Topical antimicrobials that contain silver,33 PVI, cadexomer iodine,34 or chlorhexidine are useful as topical agents in this setting to reduce multiresistant bacterial burden without generating significant antimicrobial resistance.
In conclusion, the management of infection requires knowledge of bacterial burden, how microorganisms interact with the host in a wound, and how to assess the presence of infection at the bedside.
Antibiotics by themselves are insufficient to manage infections in chronic wounds. For instance, debridement, pressure relief, and moisture-retentive dressings as treatment modalities in diabetic neuropathic ulcers can reduce the likelihood of infection to 2.5% from a baseline infection rate of 6% where traditional gauze dressings are used.35 Table 5 summarizes the management strategies that combine multiple treatment modalities with antimicrobial therapy.
1. Tammelin A, Lindholm C, Hambraeus A. Chronic ulcers and antibiotic treatment. J Wound Care 1998;7:435-7.
2. Landis S, Ryan S, Woo K, Sibbald RG. Infections in chronic wounds, in Chronic Wound Care: A clinical source book for health care professionals
, 4th edition, eds. Krasner, Rodeheaver, Sibbald; 2007.
3. Bowler PG, Davies BJ. The microbiology of acute and chronic wounds. Wounds 1999;11:72-8.
4. Noyes HE, Chi NH, Linh LT, et al. Delayed topical antimicrobials as adjuncts to systemic antibiotic therapy of war wounds: bacteriologic studies. Mil Med 1967;132:461-8.
5. Bendy RH Jr., Nuccio PA, Wolfe E, et al. Relationship of quantitative wound bacterial counts to healing of decubiti: effect of topical gentamicin. Antimicrob Agents Chemother 1964;4:147-55.
6. Howell-Jones RS, Wilson MJ, Hill KE, et al. A review of the microbiology, antibiotic usage and resistance in chronic skin wounds. J Antimicrob Chemother 2005;55:143-9.
7. Gerding DN. Foot infections in diabetic patients: the role of anaerobes. Clin Infect Dis 1995;20:S283-8.
8. Bowler PG, Davies BJ. The microbiology of infected and non-infected leg ulcers. Int J Dermatol 1999;38:573-8.
9. Bowler PG. The anaerobic and aerobic microbiology of wounds, a review. Wounds 1998;10:170-8.
10. Sheffield PJ. Tissue oxygen measurements, in Problem wounds: the role of oxygen
. eds. Davis, Hunt, Elsevier, New York, NY, 1988; 17-51.
11. Hohn DC, MacKay RD, Halliday B, et al. Effect of O2
tension on microbicidal function of leukocytes in wounds and in vitro. Surg Forum 1976;27:18-20.
12. Bowler PG. The 10(5) Bacterial growth guideline: reassessing its clinical relevance in wound healing. Ostomy Wound Manage 2003;49:44-53.
13. Sibbald RG, Woo K, Ayello EA. Increased bacterial burden and infection: the story of NERDS and STONES. Adv Skin Wound Care 2006;19:447-63.
14. Pierard-Franchimont C, Paquet P, Arrese JE, et al. Healing rate and bacterial necrotizing vasculitis in venous leg ulcers. Dermatology 1997;194:383-7.
15. Gravett A, Sterner S, Clinton JE, et al. A trial of povidone iodine in the prevention of infection in sutured lacerations. Ann Emerg Med 1987;16:167-71.
16. Woo K, Etemadi P, Coelho S, et al. Iodine solution: is it a solution for difficult-to-heal wounds? Abstract, Can Assoc Wound Care, 2007.
17. Bain AK, Pratt L. Dilute povidone-iodine solutions inhibit human skin fibroblast growth. Dermatol Surg 2002;28:210-4.
18. Fleischer W, Reimer K. Povidone-iodine in antisepsis: State of the art. Dermatology 1997;195:3-9.
19. Moberg S, Hoffman L, Grennert ML, et al. A randomized trial of cadexomer iodine in decubitus ulcers. J Am Geriatrics Soc 1983;31:462-5.
20. O'Meara S, Al-Kurdi D, Ovington LG. Antibiotics and antiseptics for venous leg ulcers. Cochrane Database Sys Rev 2008; Jan 23: CD003557.
21. Hansson C. The effects of cadexomer iodine paste in the treatment of venous leg ulcers compared with hydrocolloid dressing and paraffin gauze dressing. Int J Dermatol 1998;37:390-6.
22. Floyer C, Wilkinson JD. Treatment of venous leg ulcers with cadexomer iodine with particular reference to iodine sensitivity, Acta Chir Scand Suppl 1988;544:60-1.
23. Mertz PM, Oliveira-Gandia MF, Davis SC. The evaluation of a cadexomer iodine wound dressing on methicillin-resistant Staphylococcus aureus (MRSA) in acute wounds. Dermatol Surg 1999;25:89-93.
24. Hansson C, Faergemann J. The effect of antiseptic solutions on microorganisms in venous leg ulcer. Acta Derm Venereol 1995;75:31-3.
25. Fumal I, Braham C, Paquet P, et al. The beneficial toxicity paradox of antimicrobials in leg ulcer healing impaired by a polymicrobial flora: a proof-of-concept study. Dermatology 2002;204:70-4.
26. Sibbald RG, Browne AC, Coutts P, et al. Screening evaluation of an ionized nanocrystalline silver dressing in chronic wound care. Ostomy Wound Manage 2001;47:38-43.
27. Sibbald RG. The selective anti-inflammatory activity of prolonged release nanocrystalline silver dressing in the treatment of chronic venous leg ulcers (Acticoat). EWMA 2005, Stuttgart, Germany.
28. Karchmer AW, Gibbons GW. Foot infections in diabetics: evaluation and management. Curr Clin Topics Inf Dis 1994;14:1-22.
29. Armstrong DG, Liswood PJ, Todd WF. 1995 William J. Stickel Bronze Award. Prevalence of mixed infections in the diabetic pedal wound. A retrospective review of 112 infections. J Am Podiatr Med Assoc 1995;85:533-7.
30. Fridkin SK, Hageman JC, Morrison M, et al. Methicillin-resistant Staphylococcus aureus disease in three communities. N Engl J Med 2005;352:1436-44.
31. Pittet D. Improving adherence to hand hygiene practice: a multidisciplinary approach. Emerg Infect Dis 2001;7:234-42.
32. Vasquez JE, Walker ES, Franzus BW, et al. The epidemiology of mupirocin resistance among MRSA at a Veterans' Affairs hospital, Infect Control Hosp Epidemiol 2000;21:459-64.
33. Edwards-Jones V. Antimicrobial and barrier effects of silver against methicillin-resistant Staphylococcus aureus. J Wound Care 2006;15:285-90.
34. Mertz PM, Oliviera-Gandia MF, Davis SC. The evaluation of a cadexomer iodine wound dressing on methicillin resistant Staphylococcus aureus (MRSA) in acute wounds. Dermatol Surg 1999;25:89-93.
35. Boulton AJ, Meneses P, Ennis WJ. Diabetic foot ulcers: A framework for prevention and care. Wound Repair Regen 1999;7:7-16.
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