Annals of Plastic Surgery

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Annals of Plastic Surgery:
doi: 10.1097/SAP.0b013e31826a1ae2

Evaluating the Effects of Subclinical, Cyclic Ischemia-Reperfusion Injury on Wound Healing Using a Novel Device in the Rabbit Ear

Steinberg, Jordan P. MD, PhD; Gurjala, Anandev N. MD, MS; Jia, Shengxian MD, PhD; Hong, Seok Jong PhD; Galiano, Robert D. MD; Mustoe, Thomas A. MD

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Objective: This study aimed to evaluate the effect of cyclic ischemia-reperfusion (IR) injury on wound healing using a novel rabbit ear model.

Materials and Methods: A lightweight clamp apparatus was developed for reversible occlusion of the central ear artery. Ventral ear wounds were analyzed postoperatively for epithelialization and granulation as well as gene expression after 3 consecutive days of IR cycling.

Results: By postoperative day #7, ears showed no gross tissue necrosis, but histologic analysis of wounds confirmed a significant impairment in epithelial and granulation tissue gaps as well as total epithelial and granulation tissue areas (P < 0.001). Quantitative polymerase chain reaction analysis of IR wounds indicated significant up-regulation of heat shock protein-70 and down-regulation of superoxide dismutase 1 relative to sham controls (P < 0.05).

Conclusions: A novel rabbit ear model for the induction of subclinical, cyclic IR injury in cutaneous tissue has been developed that will serve as a valuable tool for the testing of new therapeutics.

© 2014 by Lippincott Williams & Wilkins


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