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The Effect of Centrifugation Condition on Mature Adipocytes and Adipose Stem Cell Viability

Son, Daegu MD, PhD*; Choi, Taehyun MD, PhD; Yeo, Hyeonjung MD*; Kim, Junhyung MD, PhD*; Han, Kihwan MD, PhD*

Annals of Plastic Surgery:
doi: 10.1097/SAP.0b013e318268a85d
Research
Abstract

Abstract: Different researchers have recommended different lipoaspirate centrifugation speeds and times, probably due to the limits in fat cell viability assays. We assessed fat cell viability using a fluorescein diacetate and propidium iodide (FDA-PI) stain and 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) assay after harvesting syringe liposuction and spun with different centrifugation speeds to determine the optimal conditions. Lipoaspirates, harvested from 13 donors, were transferred into a centrifuge tube and spun at 1000, 3000, and 4000 rpm for 3 minutes. Mature adipocytes and adipose stem cells were isolated and tested with a direct counting of FDA-PI-stained cells under fluorescence microscope and XTT assay. We incubated adipocytes and adipose stem cells for 1 day and 3 days, and we compared both of them with fresh samples to evaluate the influence of culturing condition on fat cell viability. Centrifugation speeds from 1000 rpm to 4000 rpm for 3 minutes showed no change in the percentage of adipocytes and adipose stem cell viability not only in the fresh samples but also in the cultured samples (1 day and 3 days). Centrifugation speeds under 4000 rpm do not change the percentage of fat cell viability. To differentiate viable cells from dying or dead mature adipocytes and oil accurately, combinations of viability tests are essential.

Author Information

From the *Department of Plastic and Reconstructive Surgery, Keimyung University School of Medicine, Daegu; and †Department of Plastic and Reconstructive Surgery, Seoul National University College of Medicine, Seoul, South Korea.

Received April 12, 2012, and accepted for publication, after revision, July 8, 2012.

Conflicts of interest and sources of funding: The present research was conducted through the Bisa Research Grant of Keimyung University in 2007.

Reprints: Daegu Son, MD, PhD, Department of Plastic and Reconstructive Surgery, Keimyung University School of Medicine, 194 Dongsan-dong, Daegu, South Korea. E-mail: handson@dsmc.or.kr.

© 2014 by Lippincott Williams & Wilkins