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Modulation of Murine Alzheimer Pathogenesis and Behavior by Surgery

Tang, Junxia X. PhD; Mardini, Feras BS; Janik, Luke S. MD; Garrity, Sean T. BS; Li, Rosie Q. BS; Bachlani, Gulnaz BS; Eckenhoff, Roderic G. MD; Eckenhoff, Maryellen F. PhD

doi: 10.1097/SLA.0b013e318269d623
Original Articles

Objective: Previous research suggests that a link between anesthetic exposure and Alzheimer disease exists. Because anesthetics are rarely given alone, we ask whether addition of surgery further modulates Alzheimer disease.

Background: Cognitive dysfunction occurs after surgery in humans. Anesthesia alone produces cognitive decline in both older wild-type (WT) mice and rats, and the addition of surgery produces transient decline in young, adult WT mice. Because neuroinflammation has been implicated and occurs early in Alzheimer disease, we hypothesized that the neuroinflammatory stress associated with surgery would accelerate the progression of Alzheimer disease.

Methods: Cecal ligation and excision were performed on presymptomatic 5- to 11-month-old triple-transgenic Alzheimer disease (3×TgAD) and C57BL/6 WT mice under desflurane anesthesia. Surgery animals were compared with aged-matched 3×TgAD and WT mice exposed to air or desflurane alone. Cognitive function was assessed via Morris water maze at 2 and 13 weeks postoperatively. Amyloid and tau pathology and inflammation and synaptic markers were quantified with immunohistochemistry, Luminex assay, enzyme-linked immunosorbent assay, or Western blot assays.

Results: A significant cognitive impairment in 3×TgAD mice that underwent surgery compared with air or desflurane controls persisted to at least 14 weeks after surgery. Microglial activation, amyloidopathy, and tauopathy were enhanced by surgery as compared with desflurane alone. No differences between surgery, anesthetic, or air controls were detected in WT mice

Conclusions: Surgery causes a durable increment in Alzheimer pathogenesis, primarily through a transient activation of neuroinflammation.

Abdominal surgery was performed on presymptomatic Alzheimer transgenic and wild-type mice, which were assessed at 2 and 13 weeks after the procedure. Surgery, but not anesthesia alone, enhanced both Alzheimer pathogenesis and cognitive dysfunction in the brain made vulnerable by Alzheimer disease up to 3 months postsurgery, primarily through the transient activation of neuroinflammation.

From the Department of Anesthesiology and Critical Care, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA.

Reprints: Maryellen F. Eckenhoff, PhD, Department of Anesthesiology and Critical Care, Perelman School of Medicine, University of Pennsylvania, 305 John Morgan Bldg, 3620 Hamilton Walk, Philadelphia, PA 19104. E-mail: maryellen.eckenhoff@uphs.upenn.edu.

J.X.T. and F.M. contributed equally.

Disclosure: The authors declare no conflicts of interest. This study was funded by the National Institutes of Health NIA AG031742 (R.G.E.) and the Austin Lamont Endowment Fund (R.G.E.).

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© 2013 Lippincott Williams & Wilkins, Inc.