Annals of Surgery

Skip Navigation LinksHome > December 2010 - Volume 252 - Issue 6 > B7–H3 Ligand Expression by Primary Breast Cancer and Associa...
Annals of Surgery:
doi: 10.1097/SLA.0b013e3181f1939d
Original Articles: ORIGINAL STUDY

B7–H3 Ligand Expression by Primary Breast Cancer and Associated With Regional Nodal Metastasis

Arigami, Takaaki MD, PhD*; Narita, Norihiko MD, PhD*; Mizuno, Ryuichi MD, PhD*; Nguyen, Linhda BS*; Ye, Xing MSc; Chung, Alice MD; Giuliano, Armando E. MD; Hoon, Dave S. B. MSc, PhD*

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Objective: B7 ligand family members have been shown to be important immunoregulatory factors in host tumor immune responses. We hypothesized that B7–H3, a coinhibitory factor, is expressed by primary breast cancer cells and associated with metastasis to regional tumor-draining lymph nodes.

Experimental Design: American Joint Committee on Cancer stage I to III primary breast cancers (n = 82) and normal breast specimens (n = 17) were assessed for B7–H3 expression using paraffin-embedded archival tissues. B7–H3 expression by breast cancer cells was assessed by a quantitative real-time reverse transcription-polymerase chain reaction, and B7–H3 protein expression was evaluated using immunohistochemistry.

Results: B7–H3 mRNA expression was detected in 32 of 82 (39%) primary breast tumors but not in normal breast tissues (P = 0.0029). B7–H3 expression in primary tumors significantly correlated with increasing tumor size, American Joint Committee on Cancer stage, and lymphovascular invasion (P < 0.0001, P < 0.0001, P = 0.0071). B7–H3 expression was highly correlated to sentinel lymph node and overall number of lymph nodes with metastasis P = 0.003, and P = 0.004, respectively). In a multivariate analysis, B7–H3 mRNA expression of the primary tumor significantly predicted metastasis to regional lymph nodes (P = 0.021, and P = 0.023, respectively). Antibody staining analysis of paraffin-embedded archival tissue breast tumors and flow cytometry of breast cancer cell lines demonstrated B7–H3 protein expression.

Conclusions: B7–H3 protein expressed by primary breast cancer cells is a tumor progression factor and is associated with extent of regional nodal metastasis.

© 2010 Lippincott Williams & Wilkins, Inc.


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