In this month’s issue, Dr. George Mashour
and coauthors published a paper on the relationship between sleep and sevoflurane. We asked to tour his lab and he kindly agreed.Experimental setup
After 12 hours of sleep deprivation by gentle handling, animals were induced with sevoflurane in a chamber such as the one shown in this figure. The induction chamber is where anesthesia was induced; what is also seen is the anesthesia machine with the sevoflurane vaporizer highlighted.Maintenance of anesthesia
After induction, the animals treated with sevoflurane were under anesthesia for 6 hours. Sevoflurane anesthesia was titrated to burst suppression, which is seen in the EEG tracings. This picture shows data from two animals.EEG analysis
After anesthesia, electrophysiologic recordings were continued during natural sleep recovery and analyzed with a sleep-scoring program. These tracings suggest the state of rapid eye movement sleep. After analyzing the sleep-wake recordings, sevoflurane anesthesia and natural sleep were compared for their effects on recovery from sleep deprivation.
Any comments? Send us a note
Our tour to the lab was hosted by Dr. George Mashour