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Anesthesiology:
doi: 10.1097/ALN.0b013e318194b252
Pain Medicine

Binding of Long-lasting Local Anesthetics to Lipid Emulsions

Mazoit, Jean-Xavier M.D., Ph.D.*; Le Guen, Régine B.S.†; Beloeil, Hélène M.D., Ph.D.*; Benhamou, Dan M.D.‡

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Abstract

Background: Rapid infusion of lipid emulsion has been proposed to treat local anesthetic toxicity. The authors wanted to test the buffering properties of two commercially available emulsions made of long- and of long- and medium-chain triglycerides.
Methods: Using the shake-flask method, the authors measured the solubility and binding of racemic bupivacaine, levobupivacaine, and ropivacaine to diluted Intralipid (Fresenius Kabi, Paris, France) and Medialipide (B-Braun, Boulogne, France).
Results: The apparent distribution coefficient expressed as the ratio of mole fraction was 823 ± 198 and 320 ± 65 for racemic bupivacaine and levobupivacaine, and ropivacaine, respectively, at 500 mg in the Medialipide/buffer emulsion; and 1,870 ± 92 and 1,240 ± 14 for racemic bupivacaine and levobupivacaine, and ropivacaine, respectively, in the Intralipid/buffer emulsion. Decreasing the pH from 7.40 to 7.00 of the Medialipide/buffer emulsion led to a decrease in ratio of molar concentration from 121 ± 3.8 to 46 ± 2.8 for bupivacaine, and to a lesser extent from 51 ± 4.0 to 31 ± 1.6 for ropivacaine. The capacity of the 1% emulsions was 871 and 2,200 μm for the 1% Medialipide and Intralipid emulsions, respectively. The dissociation constant was 818 and 2,120 μm for racemic bupivacaine and levobupivacaine, and ropivacaine, respectively. Increasing the temperature from 20 to 37°C led to a greater increase in affinity for ropivacaine (55%) than for bupivacaine (27%). When the pH of the buffer was decreased from 7.40 to 7.00, the affinity was decreased by a factor of 1.68, similar for both anesthetics.
Conclusions: The solubility of long-acting local anesthetics in lipid emulsions and the high capacity of binding of these emulsions most probably explain their clinical efficacy in case of toxicity. The long-chain triglyceride emulsion Intralipid appears to be about 2.5 times more efficacious than the 50/50 medium-chain/long-chain Medialipide emulsion. Also, because of their higher hydrophobicity, racemic bupivacaine and levobupivacaine seem to clear more rapidly than ropivacaine.
TOXICITY of long-acting local anesthetics (LA) remains an important problem, despite the availability of new S-enantiomers of LAs.1 Indeed, both ropivacaine and levobupivacaine appear to be less cardiotoxic than racemic bupivacaine (rac-bupivacaine),2,3 but life-threatening events still occur.4,5 Rapid infusion of lipid emulsion has recently been proposed to treat such toxic events initially in animal studies6,7 and, more recently, in human patients with success.8,9 The mechanism of action of lipid emulsions is not fully established, but the binding property of the emulsion is likely to be the major, if not the only, mechanism by which lipid emulsion reverses the adverse effects of LAs.6,10 Rapid infusion of lipid emulsion has also been used to successfully treat other intoxications in humans11 and in animal models.12–14
Lipid emulsions used for intravenous parenteral nutrition are composed of chylomicron-like particles and liposomes.15 These emulsions are also used to deliver hydrophobic drugs such as propofol.16 It is then likely that lipid emulsions rapidly adsorb hydrophobic drugs such as bupivacaine or ropivacaine. The long-chain fatty acid emulsion Intralipid (Fresenius Kabi, Paris, France) has been almost exclusively used in the context of LA toxicity. An emulsion including medium-chain fatty acids (Medialipide; B-Braun, Boulogne, France) has been also used successfully.4
We then measured the binding capacity of two commercially available emulsions made of long- and of long- and medium-chain triglycerides to test our hypothesis that lipid emulsions mainly act by binding local anesthetic molecules.
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Materials and Methods

The solubility of LAs in two commercially available lipid emulsions was measured using the classic shake-flask method; i.e., by mixing the solutions by continuous agitation. The validity of this technique was also confirmed by using a method of cobinding with activated charcoal.17
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Drugs and Chemicals
The LAs used were rac-bupivacaine (Sigma, Saint Quentin-Fallavier, France), ropivacaine (Naropeine; AstraZeneca, Rueil-Malmaison, France), and levobupivacaine (Chirocaine; Abbott, Rungis, France). The lipid emulsions were a long-chain triglyceride emulsion (Intralipid 20%) and a medium- and long-chain triglyceride (50/50) emulsion (Medialipide 20%). Medialipide is also known as Lipofundin in Germany and as Vasolipid in Denmark, Norway, and Sweden. All experiments were conducted using a buffer solution containing (in mm) NaCl 120, NaH2PO4 10, Na2HPO4 10, and CaCl2 1, pH 7.4. The lipid emulsions were diluted in buffer at the following final concentrations: 0.5, 1, and 2%. A preliminary experiment with Medialipide emulsion diluted to a 10% concentration was also performed with 64, 128, 256, and 512 mg/l rac-bupivacaine.
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Experiments
Fig. 1
Fig. 1
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All experiments were done in triplicate at 20°C. To minimize problems related to emulsion stability with time and dilution,18 the emulsion was diluted in buffer immediately before use. The LA was then added and the emulsion was mixed by shaking for a 20-min period. A measure of binding according to the duration of shaking, done with rac-bupivacaine and ropivacaine, showed that steady state was nearly attained after 1–3 min (fig. 1). The aqueous phase was then separated by 2 centrifugation steps (15,000 g, 10 min). LAs were added at the following concentrations (considering the hydrochloride salt for all LAs): 1, 2, 4, 8 16, and 32 mg/l in the 0.5, 1, and 2% Medialipide emulsions; at 8, 16, 32, and 64 mg/l in 1% Medialipide and 1% Intralipid emulsions; and at 2, 4, 8, 15.60, 31.25, 62.50, 125, 250, 500, and 1,000 mg/l in 1% and 2% Medialipide and 1% Intralipid emulsions. The accuracy of the shake-flask method was checked using cobinding with dextran-coated charcoal (Sigma, St. Quentin-Fallavier, France) at a final concentration of 2 mg/ml and a 5% lipid emulsion. In addition, rac-bupivacaine and ropivacaine binding were measured at 2, 4, 8, 15.60, 31.25, 62.50, 125, 250, 500, and 1,000 mg/l in 2% Medialipide emulsion at pH 7.00 and pH 7.40. The effect of temperature on rac-bupivacaine and ropivacaine binding was also tested on a separate set of data at 15.60, 31.25, 62.50, 125, 250, 500, and 1,000 mg/l in 1% Medialipide and 1% Intralipid at 20 and at 37°C, respectively. The data are reported as molar concentrations. LAs were assayed using gas chromatography.19
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Statistics
Equation (Uncited)
Equation (Uncited)
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The distribution coefficient was calculated as ρ, the ratio of the mole fraction in lipid and in buffer§ and as D, the ratio of molar concentration. The relationship between free concentration (concentration in the aqueous phase) and bound concentration (concentration in the lipid phase) was also analyzed using nonlinear regression. This bound (B) versus free (F) drug concentration was modeled as
, considering a one-site model. Bmax is the maximum binding capacity, Kd is the dissociation constant, and Lip is the concentration of lipid in the emulsion. The whole data set was simultaneously fitted using mixed-effect modeling with the NONMEM software (version 6, level 1; GloboMax, LLC, Hanover, MD). We added a random parameter to each of the fixed (structural) parameters (Bmax and Kd) to account for interbatch variability. These random parameters were modeled according to an exponential equation (we made the usual assumption that fixed parameters were log-normally distributed). A combined additive and constant coefficient residual (intraindividual) error model was used. The significance of random parameters and of Bmax and Kd for each LA and each emulsion was tested using the log-likelihood ratio test. Fitting was performed using the first order conditional estimation method with interaction. Because of the asymptotic nature of fitting, P < 0.01 was considered the minimum level of significance for this part of the study. The full (initial) model was based on the hypothesis that each LA and each emulsion had different binding properties. In the final model, Bmax was only dependent on the emulsion, and Kd was only dependent on the anesthetics. Kd was also similar for levobupivacaine and rac-bupivacaine. Random parameters associated with all of these fixed parameters were always significant. The data sets corresponding to the effect of pH and to the effect of temperature were separately fitted using a full model considering different Bmax and different Kd for each emulsion and each LA. The precision (95% CI) and accuracy (bias) of fitting were estimated using a nonparametric bootstrap with 1,000 replicates, and by a visual predictive check. The bootstrap was stratified by batch. In addition to NONMEM, this part of modeling was performed with the software R (version 2.5.1; The R Foundation for Statistical Computing, Vienna, Austria)#, and the normalized prediction distribution errors procedure.20 Data are reported as the mean ± SD, or as the fitted value and 95% CI.
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Results

The preliminary experiment done with a 20% Medialipide emulsion diluted to 10% showed similar results as those obtained with more diluted emulsions, but the coefficient of variation was exceedingly high. The shake-flask technique was also compared to a technique of cobinding with activated charcoal. The two techniques yielded similar results. It was not possible to differentiate between rac-bupivacaine and levobupivacaine; i.e., stereospecificity was not observed. Binding was rapid, since steady state was nearly attained after 3 min of mixing.
Table 1
Table 1
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Fig. 2
Fig. 2
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Table 2
Table 2
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Fig. 3
Fig. 3
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Binding of LAs was almost linear, from 2 to 125 mg/l in the 0.5, 1 and 2% emulsions; i.e., from 6.16 to 385 μm for bupivacaine and from 6.42 to 401 μm for ropivacaine. The apparent distribution coefficients calculated in different ranges of concentrations are reported in table 1. Decreasing the pH of the Medialipide/buffer emulsion from 7.40 to 7.00 led to an average 40–60% decrease in the apparent distribution coefficient (table 1). Increasing the temperature from 20 to 37°C led to an increase in the apparent distribution coefficient (table 1). At higher concentrations, saturation occurred progressively (fig. 2). A one-site binding model was used to fit the free versus bound concentrations. Fitting was excellent; the visual predictive check did not show any departure of the residuals from normality (the Shapiro-Wilk test was > 0.2). As with the calculation of ρ and the ratio of molar concentration, it was not possible to differentiate between rac-bupivacaine and levobupivacaine. Bmax, the binding capacity, was different for the two emulsions, but similar for the three drugs in each emulsion group (table 2). Conversely, Kd, the dissociation constant, was different between drugs (bupivacaine vs. ropivacaine) but similar for the same drug between the Medialipide and the Intralipid emulsions. (See table, Supplemental Digital Content 1, which shows Bayesian estimates of the parameters, http://links.lww.com/A678.) Decreasing the pH of the 2% Medialipide emulsion from 7.40 to 7.00 led to a decrease in the affinity (1/Kd), but without any significant effect on the capacity: Kd was increased by a factor of 1.68 (95% CI 1.65–1.75) similar for bupivacaine and ropivacaine. Increasing the temperature from 20°C to 37°C led to an increase in affinity. Bmax was similar, whether the experiment was performed at 20°C or at 37°C. Conversely, Kd was significantly lower at 37°C than at 20°C, but the decrease was significantly higher with bupivacaine (rac-bupivacaine or levobupivacaine) than ropivacaine (table 2) (fig. 3).
Since binding was linear in the range 0.5–2%, and because a preliminary study done with the 10% Medialipide emulsion had given approximate distribution coefficients and parameters of binding similar to those measured with the low concentrated emulsions, we may speculate that the binding capacity is approximately 17 mm for the 20% Medialipide emulsion and 44 mm for the 20% Intralipid® emulsion.
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Discussion

The main finding of the study was that the two lipid emulsions were capable of binding each of the three local anesthetics tested in vitro. However, no stereospecific difference between the two enantiomers of bupivacaine was observed. Bupivacaine was more bound than ropivacaine and the difference was in close relation to the octanol/water distribution coefficient of each LA. The binding capacity of Intralipid was 2–3 times greater than that of Medialipide.
Lipid emulsions were diluted in an ionic buffer to accurately measure the concentration of LAs in the aqueous phase. Indeed, dilution in buffer is known to modify the physicochemical properties of the lipid emulsion, but these conditions were close to those encountered in the clinical situation, where dilution of the emulsion occurs rapidly in the blood stream. Both emulsions are made of chylomicron-like particles. The volume of distribution of chylomicrons has been shown to be slightly greater than the plasma volume (≈4,500 ml), with a half-life between 5 and 7 min.21 The concentration of intact droplets in plasma during a rapid infusion of lipid emulsion is then expected not to exceed 1%, even if large amounts are infused. The stability of the chylomicron-like droplets depends on their surface charge. The zeta potential (the zeta potential, which stabilizes the emulsion, is the potential surrounding the droplet at its interface with the solvent) of both Intralipid and Medialipide is between -50 and -45 mV, and the use of ionic buffer to dilute the emulsion may have increased the potential to a point where flocculation begins.18 However, this may also occur in vivo. Mixing time was limited to 20 min to reduce this phenomenon. A measure of binding according to the duration of shaking showed that steady state was attained after 1–3 min (fig. 1). This expected rapid binding process demonstrates that in clinical situations, an infusion of the emulsion may rapidly trap the circulating anesthetic molecules.
We calculated the distribution coefficient as the ratio of molar concentration in the two solvents, but also as the ratio of the mole fraction: 1 l of water contains ≈55.6 moles, whereas 1 l of the lipid fraction of Intralipid and Medialipide contains ≈4.19 and ≈4.61 moles of the mixture of phospholipids, lecithins, and glycerol, respectively; similarly, 1 l octanol contains ≈7.68 moles. Calculated in this way, the classic octanol/buffer distribution coefficient of ropivacaine (5 mm) and of bupivacaine (1 mm) at 25°C is then approximately 1,000 and 3,000 for ropivacaine and bupivacaine, respectively;22 similar to the distribution coefficient of ropivacaine and of bupivacaine in Intralipid at 20°C (approximately 1,200 and 2,000, respectively) (table 1). The ratio of the mole fraction at saturation calculated from the one-site binding model was ≈3 for the Intralipid emulsion and ≈1 for the Medialipide emulsion. However, there is a large confidence interval of the estimated capacity, and these numbers need to be interpreted with care.
The drugs distribute more in Intralipid than in Medialipide. For both drugs, the distribution ratio is 2.4–3.0 greater in Intralipid than in Medialipide, suggesting that Intralipid might be preferable to Medialipide in a clinical situation. Also, bupivacaine distributes 2.0–2.6 times more than ropivacaine in Intralipid and in Medialipide. This is close to the distribution ratio of 3 of both drugs in octanol.22 Then, the clearance of the drug exerted by infusion of a lipid emulsion is expected to be lower for ropivacaine than for bupivacaine.
At concentrations greater than 400 μm, saturation becomes significant and a one-site binding model adequately fitted the data. Interestingly, the binding capacity of the emulsion was similar for bupivacaine and ropivacaine, but about 2.5 times greater with Intralipid than with Medialipide. This ratio is similar to the distribution ratio calculated at lower concentrations. The affinity of the emulsion for the drug (1/Kd) was comparable for both emulsions, but about 2.6 times greater for bupivacaine than for ropivacaine. Like the distribution ratio calculated at lower concentrations, this ratio of affinity is not very different from the ratio of 3 (bupivacaine/ropivacaine) observed for the distribution in octanol.22 In serum, bupivacaine binds to apha-1 acid glycoprotein and to albumin. The affinity of bupivacaine to apha-1 acid glycoprotein is very high (Kd = 1.62–3.69 μm), depending on experimental conditions and the enantiomer considered.22–24 The affinity of ropivacaine to apha-1 acid glycoprotein is similar to that of bupivacaine (Kd = 2.78 μm).25 However, because apha-1 acid glycoprotein is not very abundant in the human plasma (≈20 μm), the capacity is low (42–45 μm).23–25 Conversely, serum albumin is abundant in plasma (≈640 μm), and its capacity to bind bupivacaine is large (898–1510 μm), but the affinity of bupivacaine for albumin is relatively low (Kd = 680–1200 μm).23,24 The affinity of bupivacaine and ropivacaine to Intralipid and Medialipide is close to the affinity of bupivacaine to albumin (table 2). The capacity of lipid emulsions to bind bupivacaine or ropivacaine is important, since the capacity of the 1% diluted emulsion is 1.5 to 4 times the capacity of human serum23,24: 100 ml of a 20% Intralipid emulsion has the same capacity as the amount of albumin contained in 7 to 12 l of serum.
Increasing the temperature from 20 to 37°C led to an important increase in affinity and thermal energy (diffusion) seems to drive the uptake. The capacity of both emulsions remained unchanged (tables 1 and 2). In this case, the decrease in Kd (increase in affinity) was significantly greater for ropivacaine (55%) than for bupivacaine (27%). Both emulsions have a similar negatively charged double-layer interface (the zeta potential is between -45 and -50 mV at pH 7.40). Then, part of the adsorption process of the positively charged tertiary amines may also occur at the shear plane that surrounds the droplets. When the pH of the buffer was decreased from 7.40 to 7.00, the capacity remained also unchanged, but the affinity decreased by a factor of 1.68, similar for both anesthetics. Indeed, when the pH of the buffer decreases, the zeta potential increases (the droplets are less charged), but ionization of the tertiary amines bupivacaine and ropivacaine increases. In addition to the difference in phospholipid composition, the two emulsions differ also by the size of the droplets,** which may possibly explain the difference in capacity. However, the marked decrease in affinity when ionization increases, together with the temperature-dependence of binding and the close relationship between affinity and hydrophobicity of the molecules, tends to favor the hypothesis of a predominant hydrophobic uptake.
In conclusion, the solubility of long-acting LAs in lipid emulsions and the high capacity of binding presented by these emulsions most probably explain the clinical efficacy presented by a rapid infusion in case of LA toxicity. The long-chain triglyceride emulsion Intralipid appears to be about 2.5 times more efficacious than the 50/50 medium-chain/long-chain Medialipide emulsion. Also, because of their higher hydrophobicity, rac-bupivacaine and levobupivacaine seem to be more rapidly cleared than ropivacaine. Acidosis likely lowers the binding capacity of the emulsions, and this fact needs to be kept in mind. Intoxication with other drugs might also be effectively treated if their lipid solubility is in the same range of values, but further studies are needed to ascertain this potential.
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References

1. Mulroy MF: Systemic toxicity and cardiotoxicity from local anesthetics: Incidence and preventive measures. Reg Anesth Pain Med 2002; 27:556–61

2. Bardsley H, Gristwood R, Baker H, Watson N, Nimmo W: A comparison of the cardiovascular effects of levobupivacaine and rac-bupivacaine following intravenous administration to healthy volunteers. Br J Clin Pharmacol 1998; 46:245–9

3. Knudsen K, Beckman Suurküla M, Blomberg S, Sjövall J, Edvardsson N: Central nervous and cardiovascular effects of i.v. infusions of ropivacaine, bupivacaine and placebo in volunteers. Br J Anaesth 1997; 78:507–14

4. Ludot H, Tharin JY, Belouadah M, Mazoit JX, Malinovsky JM: Successful resuscitation after ropivacaine and lidocaine-induced ventricular arrhythmia following posterior lumbar plexus block in a child. Anesth Analg 2008; 106:1572–4

5. Warren JA, Thoma RB, Georgescu A, Shah SJ: Intravenous lipid infusion in the successful resuscitation of local anesthetic-induced cardiovascular collapse after supraclavicular brachial plexus block. Anesth Analg 2008; 106:1578–80

6. Weinberg GL, VadeBoncouer T, Ramaraju GA, Garcia-Amaro MF, Cwik MJ: Pretreatment or resuscitation with a lipid infusion shifts the dose-response to bupivacaine-induced asystole in rats. Anesthesiology 1998; 88:1071–5

7. Weinberg GL, Di Gregorio G, Ripper R, Kelly K, Massad M, Edelman L, Schwartz D, Shah N, Zheng S, Feinstein DL: Resuscitation with lipid versus epinephrine in a rat model of bupivacaine overdose. Anesthesiology 2008; 108:907–13

8. Rosenblatt MA, Abel M, Fischer GW, Itzkovich CJ, Eisenkraft JB: Successful use of a 20% lipid emulsion to resuscitate a patient after a presumed bupivacaine-related cardiac arrest. Anesthesiology 2006; 105:217–8

9. Litz RJ, Popp M, Stehr SN, Koch T: Successful resuscitation of a patient with ropivacaine-induced asystole after axillary plexus block using lipid infusion. Anaesthesia 2006; 61:800–1

10. Weinberg GL, Ripper R, Murphy P, Edelman LB, Hoffman W, Strichartz G, Feinstein DL: Lipid infusion accelerates removal of bupivacaine and recovery from bupivacaine toxicity in the isolated rat heart. Reg Anesth Pain Med 2006; 31:296–303

11. Sirianni AJ, Osterhoudt KC, Calello DP, Muller AA, Waterhouse MR, Goodkin MB, Weinberg GL, Henretig FM: Use of lipid emulsion in the resuscitation of a patient with prolonged cardiovascular collapse after overdose of bupropion and lamotrigine. Ann Emerg Med 2008; 51:412–5

12. Tebbutt S, Harvey M, Nicholson T, Cave G: Intralipid prolongs survival in a rat model of verapamil toxicity. Acad Emerg Med 2006; 13:134–9

13. Cave G, Harvey MG, Castle CD: The role of fat emulsion therapy in a rodent model of propranolol toxicity: A preliminary study. J Med Toxicol 2006; 2:4–7

14. Harvey M, Cave G: Intralipid outperforms sodium bicarbonate in a rabbit model of clomipramine toxicity. Ann Emerg Med 2007; 49:178–85

15. Férézou J, Gulik A, Domingo N, Milliat F, Dedieu JC, Dunel-Erb S, Chevalier C, Bach AC: Intralipid 10%: Physicochemical characterization. Nutrition 2001; 17:930–3

16. Han J, Davis SS, Washington C: Physical properties and stability of two emulsion formulations of propofol. Int J Pharm 2001; 215:207–20

17. Mazoit JX, Samii K: Binding of propofol to blood components: Implications for pharmacokinetics and for pharmacodynamics. Br J Clin Pharmacol 1999; 47:35–42

18. Washington C. Stability of lipid emulsions for drug delivery. Adv Drug Deliv Rev 1996; 20:131–45

19. Theissen O, Boileau S, Cornet C, Mazoit JX, Borrelly J, Feldman L, Laxenaire MC: Analgesia after thoracotomy by extrapleural administration of continuous bupivacaine. (Article in French) Ann Fr Anesth Reanim 1993; 12:265–72

20. Comets E, Brendel K, Mentré F: Computing normalised prediction distribution errors to evaluate nonlinear mixed-effect models: The npde add-on package for R. Comput Methods Programs Biomed 2008; 90:154–66

21. Park Y, Damron BD, Miles JM, Harris WS: Measurement of human chylomicron triglyceride clearance with a labeled commercial lipid emulsion. Lipids 2001; 36:115–20

22. Strichartz GR, Sanchez V, Arthur GR, Chafetz R, Martin D: Fundamental properties of local anesthetics. II. Measured octanol:buffer partition coefficients and pKa values of clinically used drugs. Anesth Analg 1990; 71:158–70

23. Denson D, Coyle D, Thompson G, Myers J: Alpha 1-acid glycoprotein and albumin in human serum bupivacaine binding. Clin Pharmacol Ther 1984; 35:409–15

24. Mazoit JX, Cao LS, Samii K: Binding of bupivacaine to human serum proteins, isolated albumin and isolated alpha-1-acid glycoprotein. Differences between the two enantiomers are partly due to cooperativity. J Pharmacol Exp Ther 1996; 276:109–15

25. Taheri S, Cogswell 3rd, LP Gent A, Strichartz GR: Hydrophobic and ionic factors in the binding of local anesthetics to the major variant of human alpha1-acid glycoprotein. J Pharmacol Exp Ther 2003; 304:71–80

§ The mole fraction in lipid emulsion is the proportion of anesthetic molecules to the total number of molecules of lipids and anesthetics in the emulsion. The ratio of mole fraction is then the ratio of the mole fraction of local anesthetic molecules in lipids to the mole fraction of anesthetic molecules in buffer. Cited Here...
∥ Sheiner LB, Beal SL, Boeckmann AJ. NONMEM Project Group, University of California at San Francisco, San Francisco, California 1990 (at http://www.globomax.net/products/nonmem.cfm accessed 10/23/2008). Cited Here...
# The R Foundation for Statistical Computing, Vienna University of Technology, Vienna Austria (at http://www.r-project.org/ accessed 10/23/2008). Cited Here...
** Wabel C. Influence of Lecithin on Structure and Stability of Parenteral Fat Emulsions. Ph D dissertation. 07 1998. Friedrich-Alexander-Universität Erlangen-Nürnberg. (at http://www2.chemie.uni-erlangen.de/services/dissonline/data/dissertation/Christoph_Wabel/html/ accessed 10/23/2008). Cited Here...

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