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Anesthesiology:
Pain and Regional Anesthesia

Meperidine Suppresses the Excitability of Spinal Dorsal Horn Neurons

Wolff, Matthias Dr. med.*; Olschewski, Andrea PD Dr. Dr.†; Vogel, Werner Prof. Dr.‡; Hempelmann, Gunter Prof. Dr. Dr. h.c.§

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Abstract

Background: In addition to local anesthetics, meperidine has been successfully used for local anesthesia. When applied intrathecally, the dorsal horn neurons of the superficial laminae are exposed to high concentrations of meperidine. These cells represent an important point for the transmission of pain information. This study investigated the blocking effects of meperidine on different ionic currents of spinal dorsal horn neurons and, in particular, its impact on the generation of action potentials.
Methods: Using a combination of the patch clamp technique and the entire soma isolation method, the action of meperidine on voltage-gated Na+ and K+ currents in spinal dorsal horn neurons of rats was described. Current clamp recordings from intact neurons showed the functional relevance of the ion current blockade for the generation of action potentials.
Results: Externally applied meperidine reversibly blocked voltage-gated Na+ currents with a half-maximum inhibiting concentration (IC50) of 112 μm. During repetitive stimulation, a slight phasic block occurred. In addition, A-type K+ currents and delayed-rectifier K+ currents were affected in a dose-dependent manner, with IC50 values of 102 and 52 μm, respectively. In the current clamp mode, single action potentials were suppressed by meperidine. The firing frequency was lowered to 54% at concentrations (100 μm) insufficient for the suppression of a single action potential.
Conclusions: Meperidine inhibits the complex mechanism of generating action potentials in spinal dorsal horn neurons by the blockade of voltage-gated Na+ and K+ channels. This can contribute to the local anesthetic effect of meperidine during spinal anesthesia.
SYSTEMICALLY applied meperidine is widely used for analgesia and anesthesia. In addition, meperidine produces conduction block when applied locally to peripheral nerve, suggesting that it is a local anesthetic. 1,2 Subarachnoid application of meperidine results in spinal anesthesia 3,4 including motor blockade 4–6 sufficient for surgery of the lower limbs, 3 the perineum, 4 urologic surgery, 6,7 and cesarean delivery. 8 Meperidine is also useful for intravenous anesthesia either as a sole agent 9,10 or in combination with local anesthetics. 11,12
In in vitro experiments, meperidine blocks action potential conduction in peripheral nerves 13 and muscle 14 by inhibiting voltage-gated Na+ and K+ channels.
Spinal dorsal horn neurons receive their inputs from primary afferent terminals and therefore participate in processing the sensory information from noxious receptors. In contrast to the peripheral nerve, which only conducts the action potential in the region where local anesthetics are applied during spinal anesthesia, dorsal horn neurons must generate an action potential before transmitting it to supraspinal targets. During spinal and epidural anesthesia, the applied drugs diffuse directly into the spinal cord, 15 especially to the superficial neurons in laminae I and II, which are important for the transmission of primary sensory input such as pain and thermoception. 16–18 Although most clinical studies regarding the local anesthetic effects of meperidine deal with its effects on the spinal cord, 1,3,7,8,19–21 the majority of electrophysiologic studies were performed on peripheral nerves or on artificially expressed ion channels. 2,13,22–25 These experiments demonstrated the effects of meperidine on voltage-gated ion channels and on action potential conduction, which is explained by blockade of voltage-gated Na+ and K+ channels. However, it remains unclear how meperidine influences the generation of action potentials in superficial spinal dorsal horn neurons.
In this study, we investigated the effects of meperidine on the generation of single action potentials and on series of action potentials in spinal dorsal horn neurons of the rat. Furthermore, the effect on voltage-gated Na+ and K+ channels was investigated.
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Materials and Methods

Preparation
Experiments were performed by means of the patch clamp technique 26 on 200-μm slices cut from lumbar enlargements (L3–L6) of the spinal cord of 3- to 11-day-old rats. 27–29 The animals were rapidly decapitated, and the spinal cords were carefully cut out in ice-cold preparation solution bubbled with 95% O2–5% CO2. After removal of the pial membrane with fine forceps, the spinal cord was embedded in a preparation solution containing 2% agar cooled down to 39°C. To accelerate solidification of the agar, the beaker with the preparation was placed in ice-cold water. The agar block containing the lumbar enlargement of the spinal cord was cut out and glued to a glass stage fixed in the chamber of the tissue slicer. The spinal cord was sliced in ice-cold preparation solution under continuous bubbling. The slices were thereafter incubated for 45 min at 32°C. The procedures of animal decapitation have been reported to the local veterinarian authority and are in accordance with the German guidelines.
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Solutions
Preparation solution contained 115 mm NaCl, 5.6 mm KCl, 2 mm CaCl2, 1 mm MgCl2, 11 mm glucose, 1 mm NaH2PO4, and 25 mm NaHCO3 (pH 7.4 when bubbled with 95% O2–5% CO2). In the experimental chamber, the slices were superfused by low-Ca2+ solution to reduce spontaneous synaptic activity and to prevent activation of Ca2+ currents and Ca2+-dependent K+ currents. Low-Ca2+ solution was obtained from the preparation solution by setting the concentration of Ca2+ to 0.1 mm and increasing the concentration of Mg2+ to 5 mm (referred to as Ringer’s solution). Tetraethylammonium-containing solution (TEA solution) used for Na+ current recordings contained 95 mm NaCl, 5.6 mm KCl, 0.1 mm CaCl2, 5 mm MgCl2, 11 mm glucose, 1 mm NaH2PO4, 25 mm NaHCO3, and 20 mm TEA-Cl (pH 7.4 when bubbled with 95% O2–5% CO2). The study of K+ currents was performed in Na+-free choline-Cl solution containing 141 mm choline-Cl, 0.6 mm KCl, 0.1 mm CaCl2, 5 mm MgCl2, 11 mm glucose, and 10 mm HEPES; pH 7.4 was adjusted with 1 m KOH. KCl was then added to give a final K+ concentration of 5.6 mm.
Meperidine (Dolantin®; Aventis Pharma, Bad Soden, Germany) was directly added to control solutions. The experimental chamber with a volume of 0.4 ml was continuously perfused by external solution at a rate of 2–3 ml/min; the pH of solutions containing drug was tested and corrected to eliminate potential pH-induced effects.
The pipette solution used for K+ current recordings from intact neurons contained 5 mm NaCl, 144.4 mm KCl, 1 mm MgCl2, 3 mm EGTA, and 10 mm HEPES; pH 7.3 was adjusted with 1 m KOH. KCl was then added to give a final K+ concentration of 155 mm. The pipette solution used for K+ current recordings in experiments with isolated somata contained 5 mm NaCl, 144.4 mm KCl, 1 mm MgCl2, 3 mm EGTA, and 10 mm HEPES; pH 7.3 was adjusted with 1 m NaOH. NaCl was then added to give a final Na+ concentration of 15 mm. In experiments with Na+ currents, the pipette solution contained 5.8 mm NaCl, 134 mm CsCl, 1 mm MgCl2, 3 mm EGTA, and 10 mm HEPES; pH 7.3 was adjusted with 1 m NaOH. NaCl was then added to give a final Na+ concentration of 15 mm.
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Recording Conditions
Pipettes pulled from borosilicate glass tubes (GC 150; Clark Electromedical Instruments, Pangbourne, United Kingdom) were fire polished to give a final resistance of 3–7 MΩ. The patch clamp amplifier was an Axopatch 200B (Axon Instruments, Foster City, CA). In experiments with Na+ currents, the effective corner frequency of the low-pass filter was 5 kHz; K+ currents were filtered with 1 kHz. The frequency of digitization was twice that of the filter frequency. In current clamp mode, action potentials were filtered with 5 kHz and sampled at 10 kHz. The data were stored and analyzed using commercially available software (pCLAMP; Axon Instruments). Transients and leakage currents were digitally subtracted in all experiments using records with hyperpolarizing pulses that activated no currents. Offset potentials were nulled directly before formation of the seal. Errors in the clamped potential evoked by the series resistance of the electrode were not corrected. For currents recorded in the isolated somata, voltage errors due to resistance in series in most cases did not exceed 4 mV. All experiments were performed at room temperature of 21–23°C.
Ion currents were investigated in voltage clamp mode. Na+ currents were recorded in external TEA solution using pipettes filled with CsCl solution. They were activated by a voltage step to −20 mV after a 50-ms prepulse to −120 mV. Holding potential was −80 mV. For investigation of use-dependent blockade (30 pulses at 2, 5, and 10 Hz), no hyperpolarizing prepulse was applied. K+ currents were recorded in external choline-Cl solution. K+ currents were separated on the basis of a procedure described previously. 30 Total K+ currents activated by depolarizing steps to +40 mV after a 150-ms prepulse to −120 mV consisted of both rapidly inactivating A-type and delayed-rectifier components. A sim-ilar depolarization applied after a 150-ms prepulse to −60 mV (which almost completely inactivates KA current) elicited only a noninactivating component of K+ current, considered to be delayed-rectifier current. The amplitudes of the KDR currents were measured at the end of a 250-ms depolarizing pulse.
Action potentials were recorded in current clamp mode. To make the action potentials or trains of action potentials comparable, we kept the membrane potential at approximately −80 mV in current clamp experiments by injecting sustained depolarizing or hyperpolarizing currents through the recording electrode. The duration of the current pulse was 10 ms for single action potentials and 500 ms for series of action potentials.
To reduce the resistance in series, experiments in voltage clamp mode were performed using the method of entire soma isolation (see The Method of Entire Soma Isolation). The identification of a neuron in the spinal cord slice was followed by the isolation procedure. In isolated somata, the voltage errors due to the resistance in series were smaller than 4 mV.
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Identification of Dorsal Horn Neurons
The dorsal horn neurons were identified in spinal cord slices as multipolar cells with a soma (8- to 12-μm diameter) located in laminae I–III. Neurons were distinguished from glial cells in voltage clamp mode on the basis of a procedure described previously. 29 In all neurons studied, a large Na+ current exceeding 1 nA could be elicited; they were able to generate action potentials and sometimes showed spontaneous synaptic activity. The resting potentials measured in intact neurons lay between −84 and −50 mV.
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Method of Entire Soma Isolation
A detailed description of the entire soma isolation method has been given elsewhere. 29 Briefly, in whole cell recording mode, the entire soma of the neuron was isolated from the slice by slow withdrawal of the recording pipette. The isolated structure was classified as soma if it had lost all of its processes during isolation and preserved only 10–20% of original Na+ current recorded from the neuron in the slice before its isolation. The isolated structure was classified as soma-plus-axon complex if it contained one process and preserved more than 90% of the original Na+ current. The sound physiologic condition of an isolated structure was confirmed by a considerable increase in its input resistance (reflecting a decrease in membrane leakage conductance), by stable or even improved membrane resting potentials, and by the ability of soma-plus-axon complex to be excitable i.e., to generate action potentials.
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Statistical Analysis and Fitting
The current study is based on recordings from 20 intact neurons in the spinal cord slice and 61 isolated somata. Numerical values are given as mean ± SEM. The parameters obtained by fitting the data points are given as mean ± SE.
Data points were fitted using a nonlinear least-squares method with the equation: f(C) = 1 − RES × C/(C + IC50), where C is the blocker concentration, IC50 is the half-maximum inhibiting concentration, and RES is the fraction of current that cannot be blocked by meperidine (only needed for the KDR currents). Because the Hill coefficient was less than 1 (0.93 for INa and KA and 0.75 for KDR), in all curves, it was set to 1 to account for a 1:1 binding.
Intergroup differences were assessed by a factorial analysis of variance with post hoc analysis with the Fisher least significant difference test. The Student paired t test was used to compare the relative amplitudes of Na+ currents at the 30th pulse before and after 100 μm meperidine (phasic blockade) and threshold potentials in control and after application of meperidine. P values less than 0.05 were considered significant.
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Results

Na+ Currents
Fig. 1
Fig. 1
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Na+ currents were recorded from isolated somata of dorsal horn neurons in external TEA solution using pipettes filled with Cs+ containing solution. Externally applied meperidine at concentrations ranging from 10 to 1,000 μm reversibly blocked the peak Na+ current in a concentration-dependent manner (fig. 1A). Figure 1B depicts currents of 13 experiments normalized to control recordings and plotted to the corresponding concentration of meperidine. These dose-response curve was fitted with the Hill equation, assuming that one blocker molecule interacts with one receptor: The corresponding half-maximum inhibiting concentration (IC50) was 118 ± 5 μm. The addition of naloxone (0.1 and 1 μm, n = 6; not shown) did not influence meperidine (100 μm) block of Na+ current.
Fig. 2
Fig. 2
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Table 1
Table 1
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To investigate the use dependence of Na+ current block by meperidine, Na+ currents were activated at a frequency of 2 Hz first in control solution and then in the presence of 100 μm meperidine. The current in control and several consecutive currents recorded in 100 μm meperidine are shown in figure 2A. Figure 2B shows the peak amplitudes of the Na+ currents in control and after addition of 100 μm meperidine normalized to the amplitude of the first current recorded in control solution (n = 8). In the absence of meperidine, the amplitude of the 30th current was reduced by 25%, probably because of the insufficient recovery of Na+ channels from slow inactivation. In the presence of 100 μm meperidine, the relative amplitude was reduced from 0.75 to 0.37 (P < 0.001) at the 30th pulse. This corresponds to a current reduction of 49% caused by slow recovery from inactivation and by use dependent blockade. A similar effect could be observed at frequencies of 5 and 10 Hz (table 1).
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K+ Currents
Fig. 3
Fig. 3
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Fig. 4
Fig. 4
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K+ currents were recorded from isolated somata of spinal dorsal horn neurons in external choline-Cl solution using pipettes filled with high-K+ solution. Meperidine was applied externally at concentrations ranging from 10 to 3,000 μm. Fast inactivating KA currents were blocked by externally applied meperidine (fig. 3A). The corresponding half-maximum inhibiting concentration was 102 ± 5 μm (n = 34;fig. 3B). A slightly higher sensitivity to meperidine could be observed in KDR currents (fig. 4A). The dose-response curve was fitted with the Hill equation, giving an IC50 value of 52 ± 10 μm (n = 31;fig. 4B). Even at high concentrations of meperidine (3,000 μm), the blockade of KDR currents was not complete. The remaining current was 14 ± 3% of the KDR current in control solution. Further investigations of KDR subtypes were not performed.
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Action Potentials
Fig. 5
Fig. 5
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Table 2
Table 2
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Current clamp experiments were performed in intact neurons in the spinal cord slice using Ringer’s solution as bath and pipettes filled with high-K+ solution. Single action potentials were elicited using a 10-ms depolarizing current pulse of increasing amplitude (fig. 5 and table 2; n = 12). A concentration of 10 μm meperidine had little effect on the shape of a single action potential. Only a slight reduction of the repolarizing velocity was observed. The addition of 100 μm meperidine caused marginal changes in a single action potential. The overshoot potential was slightly reduced, and the width of the action potential increased. Most noticeably, the hyperpolarizing afterpotential disappeared. These effects were more pronounced with 300 μm meperidine. The application of 1,000 μm meperidine resulted in disappearance of the action potential; the membrane response was only passive to the stimulus current. A detailed analysis of different action potential parameters is given in table 2.
Fig. 6
Fig. 6
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Series of action potentials were elicited using a depolarizing current pulse with a duration of 500 ms. The recording in control solution at different current pulses was followed by the application of 100 μm meperidine, a concentration that produced only slight effects on a single action potential. Figure 6 shows the effect of 100 μm meperidine on the firing frequency of series of action potentials. Compared with control recordings in eight neurons, the maximum firing frequency was reduced to 54 ± 8% (n = 8). At strong current pulses eliciting the maximum firing frequency in control solution, the neuron in the meperidine containing solution lost its ability to produce series of action potentials.
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Discussion

In addition to local anesthetics in clinical practice, numerous other drugs, such as opioids, 5,8,31 ketamine, 32 and clonidine, 33 can be used to achieve local anesthesia. Among opioids, meperidine takes an exceptional position and has already successfully been used in spinal anesthesia, where it produces a strong sensory and motor blockade comparable to that of lidocaine. 20,34,35 To understand the action of intrathecally applied meperidine, it is necessary to consider its effects on different sites: (1) mixed nerves after their passage through the intervertebral foramina, (2) dorsal root ganglion, and (3) spinal cord. Many investigators have studied the action of meperidine on peripheral nerve. 13,24,36 In contrast, little is known about the local anesthetic action of meperidine on the spinal cord or the dorsal root ganglia. However, in numerous clinical studies, meperidine has been administered intrathecally. 1,4,7
In this article, we investigated the effects of meperidine on voltage-gated ion channels and on action potentials in spinal dorsal horn neurons. The major findings of this study are as follows: (1) At clinical concentra-tions 4–6,8,21 of meperidine, voltage-gated Na+ and K+ channels are inhibited. (2) In the current clamp mode, the firing frequency of trains of action potentials is decreased at concentrations insufficient for the suppression of a single action potential (100 μm). (3) Single action potentials are suppressed by meperidine (1,000 μm).
Meperidine at clinically relevant concentrations during local anesthesia exhibits both tonic and a slight use-dependent block of Na+ currents in sensory neurons of laminae I and II of the spinal cord. The IC50 value for tonic block is 118 ± 5 μm. This value is in the same range as described for local anesthetics. 30 Similar values were reported for single Na+ channels in frog nerve fibers. Bräu et al.24 found an IC50 value of 164 μm. Significantly higher concentrations were reported for the α subunit of μ1 Na+ channels expressed heterologously with rat brain β1 accessory subunit in Xenopus oocytes 2 (IC50 = 1.9 mm). In our experiments, the fraction of blocked ion channels increased during repetitive stimulation from 30% in control to 63% in 100 μm meperidine (2 Hz). This use-dependent blockade is usually explained by a higher affinity of the drug to open or inactivated Na+ channels compared with affinity to the resting state. 37–39 The blockade of Na+ currents and of single action potentials cannot be reversed by different concentrations of naloxone, indicating that the electrophysiologic effects described in this article are not mediated via opioid receptors. This is in accord with observations in peripheral nerve. 24
K+ channels play an important role in regulating the firing patterns of different neurons. In our experiments, meperidine exhibits a tonic blocking action on KA and on KDR currents with IC50 values of 102 and 52 μm, respectively. In contrast, local anesthetics such as lidocaine, bupivacaine, and mepivacaine affected KDR currents in an ambivalent manner; low concentrations (1–10 μm) enhance and higher concentrations (1 mm) block KDR current in spinal dorsal horn neurons. 40 The incomplete block of KDR currents seems to be a common feature of meperidine and local anesthetics. 41 The same effect of meperidine with a similar residuum was observed in amphibian peripheral nerves. 24 It might result from a diversity of K+ channels contributing to this current; some subtypes may not be sensitive to meperidine. Another explanation is that meperidine or local anesthetics are not completely effective in blocking these currents. Local anesthetics such as lidocaine, bupivacaine, and mepivacaine block KA currents with similar IC50 values ranging between 109 and 236 μm. 30
Single action potentials are suppressed by meperidine in a concentration-dependent manner. Low concentrations (10–300 μm) of meperidine reduce the overshoot potential, and the width of the action potential is increased. The slight reduction in the repolarization velocity and the decrease in the threshold potential is probably due the higher sensitivity of K+ currents to meperidine. At high concentrations (1,000 μm) of meperidine, total Na+ current blockade causes a complete suppression of the action potential. Lower concentrations of meperidine (100 μm; IC50 for Na+ currents) insufficient for suppression of a single action potential reduce the maximum firing frequency to 54%. A selective blockade of approximately 50% of the KDR current by 10 mm tetraethylammonium reduces the firing frequency in spinal dorsal horn neurons to 72%. A similar reduction in firing frequency (to 68%) was caused by 100 μm droperidol, blocking voltage-gated sodium channels and KDR to approximately 50%. An exclusive blockade of voltage-gated Na+ currents by tetrodotoxin was insufficient to produce a reduction in firing frequency. Olschewski et al.42 observed no reduction in firing frequency when 10 nm tetrodotoxin was applied to block approximately 50% of Na+ channels in spinal dorsal horn neurons. We conclude that the unexpected strong reduction in firing frequency by meperidine could originate from an additional block of KA currents.
It is difficult to correlate the blockade of Na+ and K+ currents with the effects on action potentials. There are different safety factors for the different channel types, meaning that only a fraction of the available ion channels are required to produce a single action potential. Madeja et al.43 have demonstrated different IC50 values for the blockade of sodium currents by tetrodotoxin (IC50 = 6.4 nmol) and the reduction of action potential amplitude by tetrodotoxin (IC50 = 104 nmol). At 100 μm meperidine, the action potential duration was not significantly increased, even though K+ currents were significantly inhibited in isolated somata. This might be due to a preferred dendritic localization of KDR currents, 44 which are unlikely involved in the repolarization of the axon hillock where the action potentials are generated. 29 Unfortunately, little is known about the safety factors for K+ channels in spinal dorsal horn neurons.
In comparison with other opioids, meperidine exhibits the strongest local anesthetic action at clinical concentrations. 45,46 This could be because of its low affinity for opioid receptors allowing the application of high concentrations of meperidine. 24 Most opioids in clinical practice have much higher affinities to opioid receptors. Therefore, they have to be applied at relatively low doses that do not exert a local anesthetic effect. 13,23 However, they will act as local anesthetics at concentrations exceeding those used in clinical settings. Fentanyl 22,47 and sufentanil 47 applied at unusually high concentrations (in the micromolar range) were also able to block nerve conduction in rabbit vagus nerve. When applied intrathecally, the usual dose of meperidine is 0.5–1 mg/kg. 4–6,8 Assuming that the volume of distribution after application of meperidine is 13 ml/kg, 21 the maximum cerebrospinal fluid concentration can be calculated at 310 μm (1 mg/kg meperidine). This concentration is in the range needed to block voltage-gated Na+ and K+ currents or single action potentials; a strong inhibiting effect on the generation of series of action potential can be expected. Because the plasma concentrations of intravenously applied meperidine are in the range of 1.3–2 μm, 48,49 it is unlikely that its systemic effects are mediated by an interaction with ion channels. For this reason, the local anesthetic effect of a single dose of subarachnoid meperidine can be explained by the local anesthetic effect of meperidine. However, an additional analgesic action of meperidine by its interaction with different transmitter systems, opioid receptors, or other ion channels could also contribute to the local anesthetic effect of meperidine.
In conclusion, we have shown that meperidine at concentrations reached after intrathecal application in the cerebrospinal fluid suppresses both voltage-gated Na+ and K+ (KA and KDR) currents. In current clamp mode, single action potentials are suppressed in a dose-dependent manner. Concentrations insufficient for blockade of a single action potential produce a strong reduction in firing frequency of spinal dorsal horn neurons. Neuronal firing was also suppressed by blockade of voltage-gated K+ channels. Therefore, block of pain transmission by meperidine should be considered a complex interaction that includes effects on different ion channels.
The authors thank Mary K. Steen-Müller, Dr. med. (Internist, Department of Internal Medicine, Justus-Liebig-University, Giessen, Germany), PD Dr. Michael E. Bräu (Anesthesiologist, Anesthesiology, Intensive Care Medicine, Pain Therapy, Justus-Liebig-University), and Philipp Heugel (Student of Medicine, Justus-Liebig-University) for critically reading the manuscript. Excellent technical assistance by Brigitte Agari (Assistant Medical Technician, Department of Physiology, Justus-Liebig-University) and Otto Becker (Precision Mechanic, Department of Physiology, Justus-Liebig-University) is gratefully acknowledged.
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References

1. Ngan Kee WD: Intrathecal pethidine: Pharmacology and clinical applications. Anaesth Intensive Care 1998; 26: 137–46

2. Wagner LE, Eaton M, Sabnis SS, Gingrich KJ: Meperidine and lidocaine block of recombinant voltage-dependent Na+ channels: Evidence that meperidine is a local anesthetic. A nesthesiology 1999; 91: 1481–90

3. Mircea N, Constantinescu C, Jianu E, Busu G, Ene C, Daschievici S, Nedelcu A, Leoveanu A: L’anesthésie sousarachnoidienne par la pethidine. Ann Fr Anesth Reanim 1982; 1: 167–71

4. Chaudhari LS, Kane DG, Shivkumar B, Kamath SK: Comparative study of intrathecal pethidine versus lignocaine as an anaesthetic and a postoperative analgesic for perianal surgery. J Postgrad Med 1996; 42: 43–5

5. Fernandez-Galinski SM, Monells J, Espadaler JM, Pol O, Puig MM: Effects of subarachnoid lidocaine, meperidine and fentanyl on somatosensory and motor evoked responses in awake humans. Acta Anaesthesiol Scand 1996; 40: 39–46

6. Grace D, Fee JP: Anaesthesia and adverse effects after intrathecal pethidine hydrochloride for urological surgery. Anaesthesia 1995; 50: 1036–40

7. Sia AT, Chow MY, Koay CK, Chong JL: Intrathecal pethidine: An alternative anaesthetic for transurethral resection of prostate? Anaesth Intensive Care 1997; 25: 650–4

8. Nguyen Thi TV, Orliaguet G, Ngu TH, Bonnet F: Spinal anesthesia with meperidine as the sole agent for cesarean delivery. Reg Anesth 1994; 19: 386–9

9. Acalovschi I, Cristea T: Intravenous regional anesthesia with meperidine. Anesth Analg 1995; 81: 539–43

10. Oldroyd GJ, Tham EJ, Power I: An investigation of the local anaesthetic effects of pethidine in volunteers. Anaesthesia 1994; 49: 503–6

11. Armstrong PJ, Morton CP, Nimmo AF: Pethidine has a local anaesthetic action on peripheral nerves in vivo: Addition to prilocaine 0.25% for intravenous regional anaesthesia in volunteers. Anaesthesia 1993; 48: 382–6

12. Reuben SS, Steinberg RB, Lurie SD, Gibson CS: A dose-response study of intravenous regional anesthesia with meperidine. Anesth Analg 1999; 88: 831–5

13. Jaffe RA, Rowe MA: A comparison of the local anesthetic effects of meperidine, fentanyl, and sufentanil on dorsal root axons. Anesth Analg 1996; 83: 776–81

14. Frank GB: Two mechanisms for the meperidine block of action potential production in frog’s skeletal muscle: Non-specific and opiate drug receptor mediated blockade. J Physiol 1975; 252: 585–601

15. Bromage PR, Joyal AC, Binney IC: Local anesthetic drugs: Penetration from the spinal extradural space into the neuraxis. Science 1963; 140: 392–4

16. Light AR, Perl ER: Reexamination of the dorsal root projection to the spinal dorsal horn including observations on the differential termination of coarse and fine fibers. J Comp Neurol 1979; 186: 117–31

17. Light AR, Trevino DL, Perl ER: Morphological features of functionally defined neurons in the marginal zone and substantia gelatinosa of the spinal dorsal horn. J Comp Neurol 1979; 186: 151–71

18. Safronov BV: Spatial distribution of NA+ and K+ channels in spinal dorsal horn neurones: Role of the soma, axon and dendrites in spike generation. Prog Neurobiol 1999; 59: 217–41

19. Famewo CE, Naguib M: Spinal anaesthesia with meperidine as the sole agent. Can Anaesth Soc J 1985; 32: 533–7

20. Acalovschi I, Ene V, Lorinczi E, Nicolaus F: Saddle block with pethidine for perineal operations. Br J Anesth 1986; 58: 1012–16

21. Nordberg G, Hansdottir V, Bondesson U, Boreus LO, Mellstrand T, Hedner T: CSF and plasma pharmacokinetics of pethidine and norpethidine in man after epidural and intrathecal administration of pethidine. Eur J Clin Pharmacol 1988; 34: 625–31

22. Power I, Brown DT, Wildsmith JA: The effect of fentanyl, meperidine and diamorphine on nerve conduction in vitro. Reg Anesth 1991; 16: 204–8

23. Kaya K, Babacan A, Beyazova M, Bolukbasi N, Akcabay M, Karadenizli Y: Effects of perineural opioids on nerve conduction of N. suralis in man. Acta Neurol Scand 1992; 85: 337–9

24. Bräu ME, Koch ED, Vogel W, Hempelmann G: Tonic blocking action of meperidine on Na+ and K+ channels in amphibian peripheral nerves. A nesthesiology 2000; 92: 147–55

25. Katchman AN, McGroary KA, Kilborn MJ, Kornick CA, Manfredi PL, Woosley RL, Ebert SN: Influence of opioid agonists on cardiac human ether-a-go-go-related gene K(+) currents. J Pharmacol Exp Ther 2002; 303: 688–94

26. Hamill OP, Marty A, Neher E, Sakmann B, Sigworth FJ: Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches. Pflugers Archiv 1981; 391: 85–100

27. Edwards FA, Konnerth A, Sakmann B, Takahashi T: A thin slice preparation for patch clamp recordings from neurones of the mammalian central nervous system. Pflugers Archiv 1989; 414: 600–12

28. Takahashi T: Membrane currents in visually identified motoneurones of neonatal rat spinal cord. J Physiol 1990; 423: 27–46

29. Safronov BV, Wolff M, Vogel W: Functional distribution of three types of Na+ channel on soma and processes of dorsal horn neurones of rat spinal cord. J Physiol 1997; 503: 371–85

30. Olschewski A, Hempelmann G, Vogel W, Safronov BV: Blockade of Na+ and K+ currents by local anesthetics in the dorsal horn neurons of the spinal cord. A nesthesiology 1998; 88: 172–9

31. D’Angelo R, Anderson MT, Philip J, Eisenach JC: Intrathecal sufentanil compared to epidural bupivacaine for labor analgesia. A nesthesiology 1994; 80: 1209–15

32. Bion JF: Intrathecal ketamine for war surgery: A preliminary study under field conditions. Anaesthesia 1984; 39: 1023–8

33. Eisenach JC, De Kock M, Klimscha W: α2-Adrenergic agonists for regional anesthesia: A clinical review of clonidine (1984–1995). A nesthesiology 1996; 85: 655–74

34. Patel D, Janardhan Y, Merai B, Robalino J, Shevde K: Comparison of intrathecal meperidine and lidocaine in endoscopic urological procedures. Can J Anaesth 1990; 37: 567–70

35. Sangarlangkarn S, Klaewtanong V, Jonglerttrakool P, Khankaew V: Meperidine as a spinal anesthetic agent: A comparison with lidocaine-glucose. Anesth Analg 1987; 66: 235–40

36. Beyazova M, Babacan A, Bilir E, Akcabay M, Kaya K, Baysal AI: Perineural pethidine: Effects of different doses on nerve conduction. Eur J Anaesthesiol 1993; 10: 353–6

37. Hille B: Ion Channels of Excitable Membranes, 3rd edition. Sunderland, Massachusetts, Sinauer Associates, 2001

38. Strichartz GR: The inhibition of sodium currents in myelinated nerve by quaternary derivatives of lidocaine. J Gen Physiol 1973; 62: 37–57

39. Courtney KR: Mechanism of frequency-dependent inhibition of sodium currents in frog myelinated nerve by the lidocaine derivative GEA. J Pharmacol Exp Ther 1975; 195: 225–36

40. Olschewski A, Wolff M, Brau ME, Hempelmann G, Vogel W, Safronov BV: Enhancement of delayed-rectifier potassium conductance by low concentrations of local anaesthetics in spinal sensory neurones. Br J Pharmacol 2002; 136: 540–9

41. Bräu ME, Vogel W, Hempelmann G: Fundamental properties of local anesthetics: Half-maximal blocking concentrations for tonic block of Na+ and K+ channels in peripheral nerve. Anesth Analg 1998; 87: 885–9

42. Olschewski A, Hempelmann G, Vogel W, Safronov BV: Suppression of potassium conductance by droperidol has influence on excitability of spinal sensory neurons. A nesthesiology 2001; 94: 280–9

43. Madeja M: Do neurons have a reserve of sodium channels for the generation of action potentials? A study on acutely isolated CA1 neurons from the guinea-pig hippocampus. Eur J Neurosci 2000; 12: 1–7

44. Wolff M, Vogel W, Safronov BV: Uneven distribution of K+ channels in soma, axon and dendrites of rat spinal neurones: Functional role of the soma in generation of action potentials. J Physiol 1998; 509: 767–76

45. Hassan HG, Pilcher CW, Akerman B, Renck H: Antinociceptive effects of localized administration of opioids compared with lidocaine. Reg Anesth 1989; 14: 138–44

46. Grant GJ, Vermeulen K, Zakowski MI, Langerman L: Perineural antinociceptive effect of opioids in a rat model. Acta Anaesthesiol Scand 2001; 45: 906–10

47. Gissen AJ, Gugino LD, Datta S, Miller J, Covino BG: Effects of fentanyl and sufentanil on peripheral mammalian nerves. Anesth Analg 1987; 66: 1272–6

48. Tamsen A, Hartvig P, Fagerlund C, Dahlstrom B: Patient-controlled analgesic therapy: II. Individual analgesic demand and analgesic plasma concentrations of pethidine in postoperative pain. Clin Pharmacokinet 1982; 7: 164–75

49. Sprigge JS, East DS, Fox GS, Ogilvie RI, Otton PE, Bevan DR: Meperidine infusion for postoperative analgesia in grossly obese patients. Can Anaesth Soc J 1982; 29: 142–7

50. Zhou FM, Hablitz JJ: Layer I neurons of rat neocortex: I. Action potential and repetitive firing properties. J Neurophysiol 1996; 76: 651–67

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