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2003 Journal Symposium Preconditioning Against Ischemic Injury

Morphine Preconditions Purkinje Cells against Cell Death under In Vitro Simulated Ischemia–Reperfusion Conditions

Lim, Young Jin M.D., Ph.D.*; Zheng, Shuqiu M.D., Ph.D.†; Zuo, Zhiyi M.D., Ph.D.‡

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Background: Morphine pretreatment via activation of δ1-opioid receptors induces cardioprotection. In this study, the authors determined whether morphine preconditioning induces ischemic tolerance in neurons.
Methods: Cerebellar brain slices from adult Sprague-Dawley rats were incubated with morphine at 0.1–10 μm in the presence or absence of various antagonists for 30 min. They were then kept in morphine- and antagonist-free buffer for 30 min before they were subjected to simulated ischemia (oxygen–glucose deprivation) for 20 min. After being recovered in oxygenated artificial cerebrospinal fluid for 5 h, they were fixed for morphologic examination to determine the percentage of undamaged Purkinje cells.
Results: The survival rate of Purkinje cells was significantly higher in slices preconditioned with morphine (≥ 0.3 μm) before the oxygen–glucose deprivation (57 ± 4% at 0.3 μm morphine) than that of the oxygen–glucose deprivation alone (39 ± 3%, P < 0.05). This morphine preconditioning–induced neuroprotection was abolished by naloxone, a non–type-selective opioid receptor antagonist, by naltrindole, a selective δ-opioid receptor antagonist, or by 7-benzylidenenaltrexone, a selective δ1-opioid receptor antagonist. However, the effects were not blocked by the μ-, κ-, or δ2-opioid receptor antagonists, β-funaltrexamine, nor-binaltorphimine, or naltriben, respectively. Morphine preconditioning–induced neuroprotection was partially blocked by the selective mitochondrial adenosine triphosphate–sensitive potassium channel antagonist, 5-hydroxydecanoate, or the mitochondrial electron transport inhibitor, myxothiazol. None of the inhibitors used in this study alone affected the simulated ischemia–induced neuronal death.
Conclusions: These data suggest that morphine preconditioning is neuroprotective. This neuroprotection may be δ1-opioid receptor dependent and may involve mitochondrial adenosine triphosphate–sensitive potassium channel activation and free radical production. Because morphine is a commonly used analgesic, morphine preconditioning may be explored further for potential clinical use to reduce ischemic brain injury.
ISCHEMIC brain injury contributes significantly to morbidity and mortality associated with common human diseases, such as stroke and brain trauma. 1,2 Although many interventions, such as hypothermia and glutamate receptor antagonists, have been explored for potential neuroprotection, 3,4 clinically effective and safe methods for reducing ischemic brain injury have not been established.
Opioids are an important class of therapeutic agents frequently used in the perioperative period for analgesia. Opioids are inhibitory neurotransmitters, and their receptors, classified mainly as δ-, μ-, and κ-opioid subtypes, are widely distributed throughout the central nervous system. 5 Recent studies, using cultured neocortical neurons from rats, suggest that opioids reduced glutamate- and hypoxia-induced neuronal death by activation of δ-opioid receptors. 6,7 Therefore, the presence of opioids during the application of these injuries provides neuroprotection.
Opioids have also been shown to induce cardioprotection when present only before ischemia. 8–11 This preconditioning-induced protection was δ1-opioid receptor dependent. 8–11 Consistent with these results, it has been reported that ischemic preconditioning, a well-known phenomenon in which brief episodes of sublethal ischemia induce a robust protection against the deleterious effects of subsequent lethal ischemia in a variety of organ systems, including brain, heart, liver, intestine, and lung, 12,13 involves activation of δ1-opioid receptors for its cardioprotection. 14 δ1-Opioid receptors have also been implicated in the hypoxic preconditioning–induced increase in survival time of mice under subsequent lethal hypoxia. 15,16
Therefore, we hypothesize that morphine, a commonly used opioid, induces ischemic tolerance in neurons and that this morphine preconditioning–induced neuroprotection is also δ-opioid receptor dependent. We used rat cerebellar slices and simulated ischemia in vitro by oxygen–glucose deprivation (OGD) to test these hypotheses. We chose to monitor the survival rate of Purkinje neurons in the study because these neurons are sensitive to ischemia and are easy to recognize in cerebellar sections, resulting in high accuracy of the data on cell injury and death.
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Materials and Methods

The animal protocol was approved by the institutional Animal Care and Use Committee at the University of Virginia (Charlottesville, Virginia). All animal experiments were performed in accordance with the National Institutes of Health Guide for the Care and Use of Laboratory Animals. 17 All efforts were made to minimize the number of animals used and their suffering.
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7-Benzylidenenaltrexone (BNTX) was purchased from Tocris Cookson (Ellisville, MO). All reagents, unless specified below, were obtained from Sigma Chemicals (St. Louis, MO).
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Preparation of Brain Slices
As previously described, 18–20 cerebellar brain slices were prepared from 2- to 3-month-old, 230- to 270-g male Sprague-Dawley rats (Hilltop, Scottsdale, PA). Rats were anesthetized with 40 mg/kg pentobarbital intraperitoneally and then decapitated. The cerebellum was removed rapidly and placed in ice-cold artificial cerebrospinal fluid (aCSF) bubbled with 5% CO2–95% O2. The aCSF contained 116 mm NaCl, 26.2 mm NaHCO3, 5.4 mm KCl, 1.8 mm CaCl2, 0.9 mm MgCl2, 0.9 mm NaH2PO4, and 5.6 mm glucose at a pH of 7.4. The cerebellum was then sectioned with a tissue slicer into 400-μm transverse slices. After sectioning, slices were placed into a tissue holder (made of plastic and with small holes in it to allow free diffusion of gases and water; this holder also helps to avoid direct gas bubbling on slices) and remained in the oxygenated aCSF at room temperature for approximately 1 h for recovery of synaptic function. 18
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Ischemia was simulated in vitro by OGD. As previously described, 18–20 cerebellar slices were subjected to OGD by transferring them into a glass beaker containing 40 ml glucose-free aCSF (also containing 1 mm dithionite, an oxygen absorbent) bubbled with 95% N2–5% CO2 (30 min of bubbling before the addition of brain slices was performed to reduce the oxygen content in the solution). The partial pressure of oxygen in the aCSF as measured by means of a Clark oxygen electrode (Cameron Instrument Co., Port Aransas, TX) was less than 0.1 mmHg. The beaker containing the slices was immersed in a water bath to keep the temperature of aCSF at 37 ± 0.2°C. After 20 min under the OGD condition, slices were transferred to oxygenated aCSF and remained in this aCSF for 5 h at 37°C before they were fixed for morphologic examination. This period was used to simulate reperfusion and also to allow cell injury and death that may not be evident immediately after the OGD episode to become apparent.
Morphine preconditioning was performed by incubating cerebellar brain slices with various concentrations (0.1–10 μm) of morphine at 37°C for 30 min. After 30 min in morphine-free aCSF at 37°C, they were then exposed to OGD. Naloxone (a non–type-selective opioid antagonist, 50 μm), β-funaltrexamine (β-FNA; a selective μ-opioid receptor antagonist, 10 μm), 21 nor-binaltorphimine (nor-BNI; a selective κ-opioid receptor antagonist, 10 μm), 22 naltrindole (a selective δ-opioid receptor antagonist, 10 μm), 23 BNTX (a selective δ1-opioid receptor antagonist, 0.5 μm), 23 naltriben (a selective δ2-opioid receptor antagonist, 0.5 μm), 24 5-hydroxydecanoate (a selective mitochondrial KATP channel blocker, 500 μm), 25 or myxothiazol (a mitochondrial electron transport inhibitor, 1 μm) 10 were present during the period from 30 min before the addition of morphine to the end of morphine pretreatment (total of 1 h). The concentration selection of these inhibitors was mainly based on previous studies. 6,8,10,11,24,25 As a control study, these inhibitors were present for the same duration of time in the incubations of some brain slices that were not preconditioned with morphine before OGD.
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Morphologic Examination
After incubation, cerebellar slices were fixed in 4% paraformaldehyde in buffered saline overnight at 4°C. The slices were then paraffin embedded and sectioned into 4-μm-thick sections. The sections were cut from an interior region of the cerebellar slices (approximately 100 μm from the edge) to avoid areas subjected to slicing trauma during slice preparation. Morphologic examination was performed after staining the brain sections with hematoxylin and eosin. The sections were examined by an observer who was blinded to the group assignment to determine the percentage of undamaged Purkinje cells (survival rate). Damaged neurons were identified if the cells had one of the following characteristics: cell swelling; vacuolization; or presence of shrunken, darkened nuclei. 26 Purkinje cells were recognized based on the morphology and the location (between the molecular and granular layers). At least 50 Purkinje cells were counted from each of the sections and cells in total three sections were counted for each experimental condition (> 150 Purkinje cells) to compute the percentages.
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Statistical Analysis
Results are presented as mean ± SEM. Each experiment included a control group and an OGD group. Each experimental condition was repeated with cerebellar slices from 9–14 rats. Statistical analysis was performed by means of Kruskal-Wallis test followed by Mann–Whitney rank sum test when appropriate. A P < 0.05 was considered significant.
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Morphine Preconditioning Dose-dependently Reduces OGD-induced Purkinje Cell Death
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Oxygen–glucose deprivation and reoxygenation induced Purkinje cell death (fig. 1). A 30-min preconditioning of the cerebellar brain slices with morphine increased the survival rate of Purkinje cells after OGD and reoxygenation (fig. 2). The protective effects of morphine preconditioning were concentration dependent and were maximal at concentrations higher than 0.3 μm (survival rates were 57 ± 4 and 39 ± 3% in slices preconditioned with 0.3 μm morphine before OGD and in slices subjected to OGD alone, respectively, n = 9, P < 0.05;fig. 2).
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δ1-Opioid Receptors Mediate Morphine Preconditioning–induced Neuroprotection
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Morphine (3 μm) preconditioning–induced neuroprotection was abolished by the non–type-selective opioid receptor antagonist naloxone (naloxone reduced survival rates from 56 ± 2% in slices with morphine preconditioning plus OGD to 39 ± 3%, n = 11, P < 0.001, which was not different from that in slices subjected to OGD alone, 36 ± 3%;fig. 3). To identify the subtypes of opioid receptors that mediate morphine preconditioning–induced neuroprotection, selective antagonists for the μ-, κ-, or δ-opioid receptors were used in the study. When they were present with morphine during the preconditioning period, only naltrindole, a δ-opioid receptor antagonist, inhibited morphine preconditioning–induced neuroprotection (naltrindole reduced survival rates from 49 ± 2% in slices with morphine preconditioning plus OGD to 28 ± 2%, n = 11, P < 0.001, which was not different from that in slices subjected to OGD alone, 27 ± 2%;fig. 3). Either β-FNA or nor-BNI, antagonists for μ- or κ-opioid receptors, respectively, did not affect morphine preconditioning–induced neuroprotection (fig. 3). Additional data using selective δ1- or δ2-opioid receptor antagonists showed that the neuroprotection induced by morphine preconditioning was abolished by the δ1-opioid receptor antagonist BNTX (BNTX reduced survival rates from 56 ± 2% in slices with morphine preconditioning plus OGD to 38 ± 3%, n = 11, P < 0.001, which was not different from that in slices subjected to OGD alone, 36 ± 3%) and was not affected by the δ2-opioid receptor antagonist naltriben (fig. 4). The presence of naloxone, β-FNA, nor-BNI, naltrindole, BNTX, or naltriben alone did not change the survival rates of Purkinje neurons under the OGD and reoxygenation conditions (figs. 3 and 4).
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Mitochondrial KATP Channels and Mitochondrial Electron Transport System May Be Involved in Morphine Preconditioning–induced Neuroprotection
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Concomitant presence of 5-hydroxydecanoate or myxothiazol, inhibitors for mitochondrial KATP channels and mitochondrial electron transport, respectively, during the 30-min exposure of cerebellar slices to morphine (3 μm) at least partially blocked morphine preconditioning–induced neuroprotection (5-hydroxydecanoate and myxothiazol reduced survival rates from 55 ± 2% in slices with morphine preconditioning plus OGD to 44 ± 3% and 44 ± 3%, respectively, n = 12, P < 0.05, which were also different from that in slices subjected to OGD alone, 34 ± 2%, n = 12, P < 0.05;fig. 5). 5-Hydroxydecanoate or myxothiazol alone had no effects on OGD-/reoxygenation-induced Purkinje cell death.
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Our study demonstrated the following major findings: (1) morphine preconditioning induced neuroprotection; (2) this neuroprotection was mediated by δ1-opioid receptors and may not depend on the activation of μ-, κ-, or δ2-opioid receptors; and (3) additional intracellular components, such as mitochondrial KATP channels and electron transport system, may also be involved in morphine preconditioning–induced neuroprotection.
An earlier study showed that ischemic preconditioning–induced cardioprotection is mediated by δ1-opioid receptors. 14 In addition, pretreatment with morphine or δ1-opioid receptor agonists also induced cardioprotection against ischemia. 8–11 In isolated rabbit heart, morphine pretreatment and ischemic preconditioning were equally cardioprotective. 27 In chick myocyte cultures, morphine or the selective δ-opioid receptor agonist BW373U86 attenuated ischemia–reperfusion injury. 8,10 The protection induced by morphine was abolished by naloxone or BNTX. 9 In isolated rat heart, activation of δ-opioid receptors also improved cardiac function. 28 These findings led us to hypothesize that morphine preconditioning might also induce neuroprotection against ischemia. To avoid the presence of morphine in brain slices during OGD, we placed brain slices in morphine-free aCSF for 30 min before OGD. Our results indicate that morphine preconditioning induced a dose-dependent neuroprotective effect and the maximal protection was achieved at morphine concentrations higher than 0.3 μm, similar to the results of a previous study using chick myocyte cultures. 8 Because the minimum effective concentrations in the blood for morphine to have analgesic effects are 0.06–0.6 μm, 29 the concentrations for morphine to induce neuroprotection in our study are clinically relevant.
We then investigated which opioid receptors were involved in morphine preconditioning–induced neuroprotection. Three major opioid receptors, μ-, κ-, and δ-receptors, and two δ subtypes of opioid receptors, δ1 and δ2, have been characterized. 5,30 Previous studies have indicated that δ1-opioid receptors are important for morphine preconditioning- and ischemic preconditioning–induced cardioprotection. 8–11,14 In addition, it has been shown that activation of neuronal δ-opioid receptors during the application of injuries (not qualified as preconditioning) protected neurons from hypoxic injury 6 or glutamate excitotoxicity 7 in rat cortical neuron cultures. Inhibition of δ-opioid receptors but not μ- or κ-opioid receptors during hypoxia caused more severe neuronal injury than hypoxia alone. 6 In whole animal experiments using [D-Pen,2 D-Pen5]-enkephalin, an δ1-opioid receptor agonist, and BNTX, it has been shown that the δ1-opioid receptors mediated the increased survival time of mice to hypoxic environments. 16 Consistent with these previous results, our data suggest that δ1-opioid receptors play an important role in morphine preconditioning–induced neuroprotection because this neuroprotection was inhibited by naloxone, a non–type-selective opioid receptor antagonist, by naltrindole, a selective δ-opioid receptor antagonist, and by BNTX, a selective δ1-opioid receptor antagonist. Our results also suggest that δ2-, μ-, or κ-receptors may not be important for morphine preconditioning–induced neuroprotection because naltriben, β-FNA, or nor-BNI, antagonists for these receptors, respectively, did not inhibit the neuroprotection. β-FNA, nor-BNI, and naltrindole have been shown to have extremely high binding affinities for their respective subtypes of opioid receptors (IC50 is in the nanomolar to picomolar range). 21,31–33 In our study, we used 10 μm β-FNA, nor-BNI, and naltrindole. We assumed that such high concentrations of these antagonists would effectively block their respective receptors. In addition, 10 μm was the highest concentration used to indicate no involvement of μ or κ receptors in opioid-induced cardioprotection and neuroprotection in the previous studies. 6,11 BNTX was reported to be approximately 10-fold less potent than naltriben to inhibit [3H]naltriben binding. 24 In this study, although the same concentrations (0.5 μm) of naltriben and BNTX were used, naltriben did not inhibit morphine preconditioning–induced neuroprotection.
After activation of δ1-opioid receptors in the cell membrane, what are the intracellular mediators for morphine preconditioning–induced neuroprotection? KATP channels, especially mitochondrial KATP channels, have been shown to mediate morphine preconditioning–induced cardioprotection. 8,9 In addition, oxygen free radicals have also been found to be important in this cardioprotection. 9,10,34 In fact, a positive feedback circle has been proposed for mitochondrial KATP channels and free radicals: free radicals activate KATP channels, and activation of KATP channels can induce free radical production. 9,10 We tested whether KATP channels and free radicals played a role in morphine preconditioning–induced neuroprotection by using 5-hydroxydecanoate, a selective mitochondrial KATP channel inhibitor with no effects on sarcolemmal KATP channels, 25 and myxothiazol, a mitochondrial site III electron transport inhibitor that can inhibit production of oxygen free radicals in mitochondria. 10,35 Our results suggest that both KATP channels and free radical production in mitochondria may be involved in morphine preconditioning–induced neuroprotection.
Therefore, our study suggests that the signaling pathway for morphine preconditioning–induced neuroprotection includes activation of δ1-opioid receptors and mitochondrial KATP channels and production of free radicals. Because it has been shown that inhibition of mitochondrial KATP channels abolished free radical production induced by morphine and the selective δ-opioid receptor agonist BW373U86 in cultured myocytes, 9,10 it is possible that mitochondrial KATP channels may be an upstream event of free radical production in opioid preconditioning–induced neuroprotection. The positive feedback between KATP channels and production of free radicals in mitochondria may then amplify the signals to induce neuroprotection.
Our experiments were performed using brain slices. Brain slices have an advantage over cultured neurons because neurons in brain slices are similarly sensitive to hypoxia and simulated ischemia as are neurons in the intact brain. 6,18–20 The brain slice model also has an advantage over the intact animal model because it is easier to directly apply various drugs and to control variables such as oxygen tension, temperature, and chemical environments in brain slices. However, in vitro models differ in many ways from intact brains. For example, the extracellular partial pressure of carbon dioxide, H+, glutamate, and lactic acid, which are important factors to determine cell death or survival after ischemia, might not change to the same degree as they would in vivo because of diffusion away from the slice, even in the interior region of the slice that was used for our cell count. Therefore, it is not appropriate to extrapolate our results directly to in vivo conditions. In addition, we evaluated cell death at 5 h after OGD because neuronal death in brain slices is manifest within 5 h after OGD, hypoxia, or overstimulation of glutamate receptors. 18–20 However, delayed cell death can take a few days to develop 1,36–38 and cannot be assessed by our slice model because neurons in the acutely prepared slices can be well preserved only for 5–10 h in vitro in oxygenated buffer containing glucose. 26,39 Therefore, our current study may have overestimated the morphine preconditioning–induced neuroprotection.
In summary, preconditioning brain slices with morphine at clinically relevant concentrations induced acute neuroprotection. This protection depends on activation of δ1-opioid receptors. Activation of KATP channels and free radical production in mitochondria may be important downstream events for morphine preconditioning–induced neuroprotection.
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Amelioration of rCBF and PbtO(2) following TBI at high altitude by hyperbaric oxygen pre-conditioning
Hu, SL; Li, F; Luo, HS; Xia, YZ; Zhang, JQ; Hu, R; Cui, GY; Meng, H; Feng, H
Neurological Research, 32(2): 173-178.
Gwak, MS; Li, L; Zuo, Z
Neuroscience, 167(2): 256-260.
Progress in Neurobiology
Ionic storm in hypoxic/ischemic stress: Can opioid receptors subside it?
Chao, DM; Xia, Y
Progress in Neurobiology, 90(4): 439-470.
Myocardial preconditioning with volatile anesthetics. General anesthesia as protective intervention?
Buchinger, H; Grundmann, U; Ziegeler, S
Anaesthesist, 54(9): 861-+.
Experimental Neurology
Role of CDK5 in neuroprotection from serum deprivation by mu-opioid receptor agonist
Wang, Y; Xie, WY; He, Y; Wang, M; Yang, YR; Zhang, Y; Yin, DM; Jordan-Sciutto, KL; Han, JS; Wang, Y
Experimental Neurology, 202(2): 313-323.
Cellular and Molecular Life Sciences
delta-Opioid receptors protect from anoxic disruption of Na+ homeostasis via Na+ channel regulation
Kang, XZ; Chao, DM; Gu, QB; Ding, GH; Wang, YW; Balboni, G; Lazarus, LH; Xia, Y
Cellular and Molecular Life Sciences, 66(): 3505-3516.
Gao, CJ; Li, JP; Wang, W; Lu, BC; Niu, L; Zhu, C; Wei, YY; Zhang, T; Wu, SX; Chai, W; Li, YQ
Neuroscience, 168(2): 531-542.
Iubmb Life
How to clean the dirtiest place in the cell: Cationic antioxidants as intramitochondrial ROS scavengers
Skulachev, VP
Iubmb Life, 57(): 305-310.
Biochemical and Biophysical Research Communications
Opioid receptor-independent protection of ischemic rat hepatocytes by morphine
Kim, JS; Lemasters, JJ
Biochemical and Biophysical Research Communications, 351(4): 958-964.
Journal of Cerebral Blood Flow and Metabolism
Cortical delta-opioid receptors potentiate K+ homeostasis during anoxia and oxygen-glucose deprivation
Chao, DM; Donnelly, DF; Feng, Y; Bazzy-Asaad, A; Xia, Y
Journal of Cerebral Blood Flow and Metabolism, 27(2): 356-368.
Physiology & Behavior
Neuroprotection and functional recovery conferred by administration of kappa- and delta1-opioid agonists in a rat model of global ischemia
Charron, C; Messier, C; Plamondon, H
Physiology & Behavior, 93(3): 502-511.
Neuroscience Letters
Inhibition of PKCgamma membrane translocation mediated morphine preconditioning-induced neuroprotection against oxygen-glucose deprivation in the hippocampus slices of mice
Liu, Y; Li, JF; Yang, L; Ji, F; Bu, XN; Zhang, N; Zhang, BX
Neuroscience Letters, 444(1): 87-91.
Chinese Medical Journal
Involvement of delta- and mu-opioid receptors in the delayed cerebral ischemic tolerance induced by repeated electroacupuncture preconditioning in rats
Xiong, LZ; Yang, J; Wang, Q; Lu, ZH
Chinese Medical Journal, 120(5): 394-399.

Nitric oxide in the rat cerebellum after hypoxia/ischemia
Rodrigo, J; Fernandez, AP; Alonso, D; Serrano, J; Fernandez-Vizarra, P; Martinez-Murillo, R; Bentura, ML; Martinez, A
Cerebellum, 3(4): 194-203.
Neuroscience Letters
Specific changes in levels of autoantibodies to glutamate and opiate receptors induced by morphine administration in rats
Granstrem, O; Adriani, W; Shumilina, M; Izykenova, G; Dambinova, S; Laviola, G
Neuroscience Letters, 403(): 1-5.
Medicina-Buenos Aires
Neuroprotection in perinatal hypoxic-ischemic encephalopathy. Effective treatment and future perspectives
Legido, A; Valencia, I; Katsetos, CD; Delivoria-Papadopoulos, M
Medicina-Buenos Aires, 67(6): 543-555.

Journal of Experimental Biology
delta-Opioid receptor antagonism induces NMDA receptor-dependent excitotoxicity in anoxic turtle cortex
Pamenter, ME; Buck, LT
Journal of Experimental Biology, 211(): 3512-3517.
Life Sciences
Endomorphins and morphine limit anoxia-reoxygenation-induced brain mitochondrial dysfunction in the mouse
Feng, Y; Lu, YW; Lin, X; Gao, YF; Zhao, QY; Li, W; Wang, R
Life Sciences, 82(): 752-763.
Journal of Neuroimmune Pharmacology
TNF Alpha Production in Morphine-Treated Human Neural Cells Is NF-kappa B-Dependent
Sawaya, BE; Deshmane, SL; Mukerjee, R; Fan, SS; Khalili, K
Journal of Neuroimmune Pharmacology, 4(1): 140-149.
Progress in Neurobiology
Cell death and proliferation in acute slices and organotypic cultures of mammalian CNS
Lossi, L; Alasia, S; Salio, C; Merighi, A
Progress in Neurobiology, 88(4): 221-245.
Effects of Fentanyl Pretreatment on Regional Cerebral Blood Flow in Focal Cerebral Ischemia in Rats
Chi, OZ; Hunter, C; Liu, X; Chokshi, SK; Weiss, HR
Pharmacology, 85(3): 153-157.
Brain Research
Isoflurane preconditioning decreases glutamate receptor overactivation-induced Purkinje neuronal injury in rat cerebellar slices
Zheng, SQ; Zuo, ZY
Brain Research, 1054(2): 143-151.
Pediatric Research
Use of opioids in asphyxiated term neonates: Effects on neuroimaging and clinical outcome
Angeles, DM; Wycliffe, N; Michelson, D; Holshouser, BA; Deming, DD; Pearce, WJ; Sowers, LC; Ashwal, S
Pediatric Research, 57(6): 873-878.
Effects of opioid antagonists and morphine in a hippocampal hypoxia/hypoglycemia model
Ammon-Treiber, S; Stolze, D; Schroder, H; Loh, H; Hollt, V
Neuropharmacology, 49(8): 1160-1169.
Plos One
delta-Opioid Receptor Activation Rescues the Functional TrkB Receptor and Protects the Brain from Ischemia-Reperfusion Injury in the Rat
Tian, XS; Guo, JC; Zhu, M; Li, MW; Wu, GC; Xia, Y
Plos One, 8(7): -.
ARTN e69252
American Journal of Therapeutics
Cardiovascular Manifestations of Sedatives and Analgesics in the Critical Care Unit
Darrouj, J; Karma, L; Arora, R
American Journal of Therapeutics, 16(4): 339-353.
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Fries, M; Weis, J; Rossaint, R
Anesthesiology, 104(1): 211.

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Postconditioning with Isoflurane Reduced Ischemia-induced Brain Injury in Rats
Lee, JJ; Li, L; Jung, H; Zuo, Z
Anesthesiology, 108(6): 1055-1062.
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In Vitro Electrophysiologic Effects of Morphine in Rabbit Ventricular Myocytes
Xiao, G; Zhou, J; Wang, G; Cao, C; Li, G; Wong, T
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Journal of Neurosurgical Anesthesiology
nPKC&epsiv; and NMDA Receptors Participate in Neuroprotection Induced by Morphine Pretreatment
Fanjun, M; Junfa, L; Bingxi, Z; Fang, J
Journal of Neurosurgical Anesthesiology, 18(2): 119-124.

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Morphine Pretreatment Provides Histologic Protection Against Ischemia-Reperfusion Injury in Rabbit Retina
Retina, 28(3): 511-517.
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