Home Current Issue Previous Issues Podcasts Online First ASA Practice Parameters CME For Authors Journal Info
Skip Navigation LinksHome > April 1999 - Volume 90 - Issue 4 > Local Anesthetic Inhibition of Baseline Potassium Channels w...
Laboratory Investigations

Local Anesthetic Inhibition of Baseline Potassium Channels with Two Pore Domains in Tandem

Kindler, Christoph H. MD; Yost, Spencer C. MD; Gray, Andrew T. MD, PhD

Free Access
Article Outline
Collapse Box

Author Information

Collapse Box


Background: Recently, a new structural family of potassium channels characterized by two pore domains in tandem within their primary amino acid sequence was identified. These tandem pore domain potassium channels are not gated by voltage and appear to be involved in the control of baseline membrane conductances. The goal of this study was to identify mechanisms of local anesthetic action on these channels.
Methods: Oocytes of Xenopus laevis were injected with cRNA from five cloned tandem pore domain baseline potassium channels (TASK, TREK‐1, TOK1, ORK1, and TWIK‐1), and the effects of several local anesthetics on the heterologously expressed channels were assayed using two‐electrode voltage‐clamp and current‐clamp techniques.
Results: Bupivacaine (1 mM) inhibited all studied tandem pore potassium channels, with TASK inhibited most potently. The potency of inhibition was directly correlated with the octanol: buffer distribution coefficient of the local anesthetic, with the exception of tetracaine, to which TASK is relatively insensitive. The approximate 50% inhibitory concentrations of TASK were 709 [micro sign]M mepivacaine, 222 [micro sign]M lidocaine, 51 [micro sign]M R(+)‐ropivacaine, 53 [micro sign]M S(‐)‐ropivacaine, 668 [micro sign]M tetracaine, 41 [micro sign]M bupivacaine, and 39 [micro sign]M etidocaine. Local anesthetics (1 mM) significantly depolarized the resting membrane potential of TASK cRNA‐injected oocytes compared with saline‐injected control oocytes (tetracaine 22 +/− 6 mV vs. 7 +/− 1 mV, respectively, and bupivacaine 31 +/− 7 mV vs. 6 +/− 4 mV).
Conclusions: Local anesthetics inhibit tandem pore domain baseline potassium channels, and they could depolarize the resting membrane potential of cells expressing these channels. Whether inhibition of these channels contributes to conduction blockade or to the adverse effects of local anesthetics remains to be determined.
LOCAL anesthetics block propagation of compound action potentials by binding to voltage‐gated sodium (Na+) channels. [1] However, increasing evidence suggests that local anesthetics also potently block potassium (K+) currents. [2] For example, it has been shown that 20 [micro sign]M bupivacaine inhibits delayed rectifier and baseline K+ currents in frog atrial myocytes, [3] and a 50% inhibitory concentration (IC50) of 22 [micro sign]M has been established for bupivacaine for the transient outward K+ current (Ito) in rat myocytes. [4] The I (to) current, which is also present in the human heart, [5] is believed to be an important contributor in repolarization of atrial myocytes. Thus, bupivacaine plasma concentrations of 4‐12 [micro sign]g/ml ([approximate] 12‐36 [micro sign]M), which can result from intravascular injection, [6] can significantly inhibit K+ currents in cardiac tissue. [3,4] However, these plasma concentrations are associated with much lower free‐drug concentrations, because lipid‐soluble local anesthetics, such as bupivacaine, are highly protein bound. [7]
K+ channel blockade by local anesthetics also may contribute to conduction block. Drachman and Strichartz [8] showed that K+ channel blockers potentiate impulse inhibition by local anesthetics. In addition, local anesthetic inhibition of baseline K+ channels expressed by thin myelinated peripheral nerves may be a component of differential nerve block. [9] These findings suggest that the effects of local anesthetics in neural tissue may extend beyond the known direct actions on voltage‐gated Na (+) channels.
Recently, we cloned a new mammalian tandem pore domain K+ channel (TASK) that is expressed in heart and brain tissue and inhibited by local anesthetics. [10] Tandem pore domain K+ channels represent a new family of ion channels that contribute to baseline membrane conductances. [10‐12] In addition, they resemble physiologic channels known to contribute to the resting membrane potential of excitable cells, such as the Aplysia S channel [13] or the flicker channel. [9] These new ion channels have four or eight transmembrane segments and two pore domains in tandem within the primary amino acid sequence. Whereas the ion conduction pathway of voltage‐gated K+ channels is thought to be composed of four pore domains of four independent channel subunits, the tandem pore domain K (+) channels presumably constitute dimers of these tandem pore domain subunits, thereby yielding a quatrefoil structure, as seen with other K+ channels. [14]
Although we have yet to identify specific inhibitors of tandem pore domain K+ channels, bupivacaine is among the most potent pharmacologic inhibitors of the TASK channel. [10] Therefore, we evaluated the mechanisms of local anesthetic inhibition of cloned tandem pore domain K (+) channels expressed by excitable tissues. First, we evaluated bupivacaine inhibition of the outwardly rectifying K+ channel TOK1, [15] the open‐rectifier K+ channel ORK1, [16] the tandem pore domains weak inwardly rectifying K+ channel TWIK‐1, [12] the outwardly rectifying TWIK‐related K+ channel TREK‐1, [17] and the TWIK‐related acid‐sensitive K+ channel TASK. [10] Second, based on the reported pH dependence of bupivacaine block of a physiologic baseline K (+) channel, [9] we studied bupivacaine inhibition of tandem pore domain K+ channels at different pH values. Third, also based on reports of agent specificity of local anesthetic inhibition of the flicker channel, [9] we evaluated possible agent‐specific effects of local anesthetics in different concentrations on these channels. Fourth, we studied the effects of stereoisomers of local anesthetics on tandem pore domain K+ channels based on reports of the stereoselectivity of local anesthetic inhibition of several ion channels. [18‐20] Finally, we evaluated the effects of local anesthetic inhibition on the resting membrane potential of cells heterologously expressing these tandem pore domain K+ channels.
Back to Top | Article Outline

Materials and Methods

Materials and Solutions
The following studies were approved by the University of California, San Francisco, Committee on Animal Research. Mature female Xenopus laevis were obtained from Nasco (Ft. Atkinson, WI). The frogs were anesthetized by submersion in 0.3% 3‐aminobenzoic acid ethyl ester for 30 min. Oocytes were removed surgically and defolliculated by treatment, for 1 h at room temperature, with 2 mg/ml collagenase A (Boehringer‐Mannheim, Indianapolis, IN) in oocyte Ringer's solution containing a high magnesium concentration (OR‐Mg; 82 mM NaCl, 2 mM KCl, 5 mM HEPES, 20 mM MgCl2, pH 7.4). After washing with OR‐Mg, followed by washing with modified Barth's solution (88 mM NaCl, 1 mM KCl, 10 mM HEPES, 7 mM NaHCO3, 1 mM CaCl2, 1 mM Ca(NO3)2, pH 7.0), stage V and VI oocytes were selected for injection. On the same day as removal, the oocytes were injected with 5‐10 ng of TASK, TREK‐1, TOK1, ORK1, or TWIK‐1 cRNA in oocyte saline (100 mM KCl and 20 mM NaCl in diethylpyrocarbonate‐treated water) or with oocyte saline alone as controls. Injected oocytes were incubated at 18 [degree sign]C in modified Barth's solution with 50 mg/ml gentamycin, 2.5 mM sodium pyruvate, 5% heat‐inactivated horse serum, and 5 mM theophylline, with gentle rotation for 2‐4 days.
We studied three local anesthetics without a piperidine group (lidocaine, etidocaine, and tetracaine) and four with a piperidine group (mepivacaine, R(+)‐ropivacaine, S(‐)‐ropivacaine, and bupivacaine). We also studied the permanently neutral local anesthetic ethyl‐p‐amino‐benzoate (benzocaine). Lidocaine, bupivacaine, tetracaine, and benzocaine were purchased from Sigma Chemical Company (St. Louis, MO). Mepivacaine, R(+)‐and S(‐)‐ ropivacaine, and etidocaine were provided by ASTRA Pharmaceuticals (Sodertalje, Sweden). Stock solutions of local anesthetics (10 mM lidocaine, 10 mM mepivacaine, 10 mM tetracaine, 3 mM bupivacaine, 1 mM R(+)‐ and 1 mM S(‐)‐ropivacaine, and 1 mM etidocaine) were prepared in frog Ringer's solution (115 mM NaCl, 2.5 mM KCl, 1.8 mM CaCl2, 10 mM HEPES, pH 7.6) and maintained at 4 [degree sign]C for no more than 4 weeks. A stock solution of 1 M benzocaine in ethanol was prepared immediately before the experiments.
TASK, TREK‐1, TOK1, ORK1, and TWIK‐1 plasmids were linearized by restriction digestion with Xho I for TASK, HindIII for TREK‐1, Not I for TOK1 and ORK1, and BamH I for TWIK‐1. After linearization, plasmids were purified with phenol and chloroform and used as templates. Capped transcript was prepared using T3 (TASK) and T7 (TREK‐1, TOK1, ORK1, TWIK‐1) mMessage mMachines (Ambion, Austin, TX). cRNA was precipitated with lithium chloride and resuspended in oocyte saline to a final concentration of approximately 0.5 mg/ml.
Back to Top | Article Outline
Two‐electrode Voltage‐clamp and Current‐clamp Recording
Oocytes were studied by two microelectrode voltage‐clamp and current‐clamp techniques using an Axoclamp 2A amplifier (Axon Instruments, Foster City, CA). Microelectrodes (0.3‐1.5 M Omega) were backfilled with 3 M KCl. For the voltage‐clamp experiments, the holding potential was ‐80 mV. Voltage pulse protocols used 1‐s steps ranging in most experiments from ‐140 to +40 mV in 20‐mV increments, with 1.5‐s interpulse intervals. Electrophysiologic studies were performed at room temperature (20‐23 [degree sign]C) in a 25‐[micro sign]l recording chamber superfused with frog Ringer's solution at a rate of approximately 4 to 5 ml/min. Local anesthetic solutions were applied for 2‐4 min before voltage pulse protocols, and washout experiments were performed after 2‐4 min of superfusion with local anesthetic‐free frog Ringer's solution. Similar experiments with anesthetic solution at a different pH were performed with a washout period between changes of the various treatment solutions. Saline‐injected oocytes used as controls underwent the same treatments as cRNA‐injected oocytes.
For most experiments, current signals were low‐pass filtered (eight‐pole Bessel filter; Frequency Devices, Haverhill, MA) at 40 Hz, sampled at 100 Hz, digitized, and stored on a Power Macintosh 7100 computer (Apple Computers, Cupertino, CA) using data acquisition software (ADInstruments, Milford, MA). To quantify responses, leakage currents of saline‐injected oocytes were averaged and subtracted from currents of cRNA‐injected oocytes. Ion currents recorded during superfusion with local anesthetics were normalized to averaged currents measured with drug‐free frog Ringer's solution before and after the application of local anesthetics.
TWIK‐1 cRNA‐injected oocytes were studied when their resting membrane potential was more negative than ‐55 mV and when reversible barium inhibition (1 mM) was observed. TWIK‐1 cRNA‐injected oocytes that did not fulfill these criteria were excluded.
Experiments measuring changes in the membrane potentials of oocytes were performed with the same equipment using the current‐clamp mode.
Back to Top | Article Outline
Data Analysis and Statistics
Equation 1
Equation 1
Image Tools
Except when stated otherwise, results are expressed as the mean +/− SD and n values indicate the number of oocytes studied. Octanol:buffer distribution coefficients (Q) for local anesthetics were calculated according to the formula Equation 1 where P+ and PO are the octanol:buffer partition coefficients for the charged and neutral species of the local anesthetic, respectively, and [Greek small letter beta] = 10 sup (pKa‐pH) as described by Strichartz et al. [21]
Equation 2
Equation 2
Image Tools
The approximate IC50 values for the inhibition of TASK by various local anesthetics were estimated by fitting the logistic transformed response data to the Hill Equation usinga linear least‐squares fitting procedure (JMP; SAS Institute Inc., Cary, NC) Equation 2 where R = response (ILA/IControl), IC50 = the concentration at which 50% inhibition is observed, [LA] = the concentration of local anesthetic, and n = the Hill coefficient.
The voltage dependence of local anesthetic inhibition of TASK currents was analyzed by normalization of leakage‐subtracted currents in the presence of local anesthetic to matching controls to yield a fractional block f at each voltage. Data at potentials greater than ‐40 mV were fit to the Woodhull Equation byregression to estimate the Woodhull coefficient [Greek small letter delta] (the fraction of the electric field that the blocker experiences). [22] For calculations of the Woodhull coefficients, the effective charge z was assumed to be +1.
We used paired or unpaired Student t tests to identify differences in data in two groups when appropriate. To identify differences in more than two groups, we used one‐way analysis of variance followed by the Student‐Newman‐Keuls test for multiple comparisons. Significance was defined as P < 0.05.
Back to Top | Article Outline


Bupivacaine Inhibition of Tandem Pore Domain K+ Channels
Figure 1
Figure 1
Image Tools
We evaluated the sensitivity of cloned tandem pore domain K+ channels to 1 mM bupivacaine. Figure 1 shows the current‐voltage relations of TASK, TREK‐1, TOK1, ORK1, and TWIK‐1 cRNA‐injected oocytes in the presence and absence of 1 mM bupivacaine. All tandem pore domain K+ channels were inhibited by bupivacaine, with TASK, TREK‐1, and TOK1 showing the greatest inhibition. TWIK‐1 showed inhibition of outward and inward currents by 1 mM bupivacaine.
Because external pH in the range of 4.3 to 8 has no effect on TOK1 activity, [10,23] the TOK1 clone was used to investigate the effect of pH alteration on local anesthetic block of a tandem pore domain K+ channel. TOK1 currents at the end of the depolarizing steps from ‐80 ‐ +40 m V were measured first without a local anesthetic at pH 7.6 and pH 6.4. In the presence of 1 mM bupivacaine, the inhibition of TOK1 was significantly greater at pH 7.6 (61 +/− 15%, n = 9) than at pH 6.4 (17 +/− 2%, n = 4; P < 0.05). Similar experiments were performed with TASK at three different extracellular pH levels and the relative responses were normalized to the corresponding pH. The inhibition of TASK currents by 10 [micro sign]M bupivacaine also was significantly greater at higher pH values (pH 8.4: 28 +/− 5%, n = 3; pH 7.6: 23 +/− 3%, n = 5; and pH 7.0: 10 +/− 3%, n = 4, respectively; P < 0.05).
Back to Top | Article Outline
Dose‐dependent Block of Tandem Pore Domain K+ Channels by Local Anesthetics
Figure 2
Figure 2
Image Tools
(Figure 2) shows current tracings from a TASK cRNA‐injected oocyte obtained in the absence and presence of 1 mM lidocaine during application of 1‐s voltage steps from ‐120‐+40 mV in 20‐mV increments (holding potential, ‐80 mV). TASK currents activated instantaneously and did not inactivate throughout the voltage pulse, with or without lidocaine superfusing the oocyte. Inhibition of the currents was fully reversible after washout.
Figure 3
Figure 3
Image Tools
Table 1
Table 1
Image Tools
TASK currents were inhibited in a dose‐dependent manner by all the local anesthetics studied (Figure 3). Table 1 summarizes the approximate IC50 values for the inhibition of TASK by various local anesthetics. The mean Hill coefficient (Equation 2 in Materials and Methods) for all local anesthetics studied was close to unity (0.8 +/− 0.2). Woodhull coefficients [Greek small letter delta] for local anesthetic inhibition of TASK currents were small and ranged from 0.04 for 10 [micro sign]M etidocaine to 0.14 for 10 [micro sign]M S(‐)‐ropivacaine, implying that the voltage‐dependence of local anesthetic inhibition is not strong. The Woodhull coefficient [Greek small letter delta] for 10 [micro sign]M bupivacaine was 0.12, which is similar to that described for the human cardiac K+ channel Kv1.5. [20] The percentage of inhibition of TASK currents by 10 [micro sign]M bupivacaine increased from 23 +/− 3% at ‐20 mV to 29 +/− 5% at +40 mV (n = 8; P < 0.05). Such an increase of inhibition in a voltage‐dependent manner is expected if bupivacaine reaches the TASK channel from inside cells. With the exception of tetracaine, the potency of a local anesthetic to inhibit TASK currents was correlated directly with the octanol:buffer distribution coefficient (R2 = 0.78, P < 0.05).
The permanently neutral local anesthetic benzocaine at doses of 1 mM and 100 [micro sign]M also inhibited TASK currents by 51 +/− 6% (n = 5) and 19 +/− 6% (n = 4), respectively. Because of the poor solubility of benzocaine in aqueous solutions, we dissolved the higher concentration with ethanol, 0.1%. Ethanol, 0.1%, used alone as control inhibited TASK currents by 12 +/− 5% (n = 6), which is significantly less than the inhibition observed with 1 mM benzocaine dissolved with ethanol, 0.1%.
Back to Top | Article Outline
Inhibition of TASK by the stereoisomers of ropivacaine, R(+) and S(‐), was studied at 10 [micro sign]M, 100 [micro sign]M, and 1 mM. No significant difference in the inhibition produced by the isomers was observed. The inhibition of TASK currents by R(+)‐ropivacaine was 18 +/− 5, 55 +/− 7, and 94 +/− 5%, whereas S(‐)‐ropivacaine inhibited the currents by 19 +/− 6 (n = 11), 50 +/− 7 (n = 7), and 93 +/− 5% (n = 3) at the different concentrations.
Back to Top | Article Outline
TASK Modulation of the Resting Membrane Potential
Figure 4
Figure 4
Image Tools
We studied the effects of various local anesthetics on the resting membrane potential of TASK cRNA‐injected oocytes. Local anesthetics caused an immediate and reversible depolarization, defined as the resting membrane potential during treatment with local anesthetic minus pretreatment values. Figure 4A shows tracings of membrane potentials before, during, and after treatment of TASK cRNA‐injected oocytes and saline‐injected oocytes (controls) with either 1 mM tetracaine or 1 mM bupivacaine. The bupivacaine‐induced depolarization of TASK cRNA‐injected oocytes (31 +/− 7 mV; n = 9) and the tetracaine‐induced depolarization of TASK cRNA‐injected oocytes (22 +/− 6 mV; n = 4) were significantly greater than the corresponding depolarizations of saline‐injected oocytes (tetracaine = 7 +/− 1 mV and bupivacaine = 6 +/− 4 mV, respectively; Figure 4B). Mepivacaine, 1 mM, also was tested and showed a depolarization of the resting membrane potential of TASK cRNA‐injected oocytes of approximately 20 mV. Because the magnitude of the depolarization induced in oocytes by local anesthetics could depend on resting membrane potential, control oocytes were hyperpolarized by constant current injection to the more negative membrane potential of ‐70 mV observed with TASK‐expressing oocytes. In these experiments, 1 mM bupivacaine still only depolarized the membrane potential of the control oocytes 7 +/− 6 mV (n = 7). The small depolarizations by local anesthetics at the 1‐mM concentration observed in saline‐injected oocytes may have resulted from inhibition of endogenous baseline K+ channels, which have been described previously. [24] Extracellular alkalinity (pH 8.4), which is known to potentiate TASK currents, [10] hyperpolarized TASK cRNA‐injected oocytes (‐4 +/− 1 mV; n = 4) but did not affect saline‐injected oocytes (‐1 +/− 2 mV; n = 8) (P < 0.05).
Back to Top | Article Outline


Recently, a new family of K+ channels characterized by two pore domains in tandem within their primary amino acid sequence has been identified. [15] Six members of this new family have now been cloned: TOK1 [15] (yeast), ORK1 [16] (Drosophila), TWIK‐1 [12,25] (mouse, human), TREK‐1 [17] (mouse), TASK [10,11,26] (rat, human, mouse), and TRAAK [27] (mouse). The tandem pore domain K+ channels are expressed in most tissues and are particularly abundant in the brain, heart, and lung, but they are also found in the kidney, pancreas, and liver. These tandem pore domain K+ channels are not voltage gated and are believed to be important contributors, along with inward rectifiers, to baseline or leak potassium conductances. [10‐12] Therefore, modulators of these channels will influence the resting membrane potential. A native K+ channel similar to TASK with respect to inhibition by acidity, local anesthetics, and Zn2+ has been described as the flicker channel of thin myelinated nerves. [28] Local anesthetics applied to isolated membrane patches from myelinated nerves that express the flicker channel indeed cause depolarization. [9] However, the affinity of bupivacaine for the flicker channel is extremely high (220 nM) compared with TASK (41 [micro sign]M). Other known physiologic baseline K+ channels include the K+ channel in myelinated axons, [29] the muscarine‐sensitive channel in sympathetic ganglia, [30] and K+ channels expressed by corneal endothelia [31] and pancreatic acinar cells. [32] Whether these physiologic baseline K+ channels also are inhibited by local anesthetics remains to be investigated.
Back to Top | Article Outline
Local Anesthetic Inhibition of Tandem Pore Domain K+ Channels
We studied currently published cloned tandem pore domain K+ channels for sensitivity to local anesthetics. Bupivacaine in a dose of 1 mM inhibited the currents of TASK, TREK‐1, TOK1, ORK1, and TWIK‐1, but had no effect on the current‐voltage relation of control saline‐injected oocytes (Figure 1). The open rectifier TASK, the currents of which satisfy the Goldman‐Hodgkin‐Katz current Equation foran open channel, and the outward rectifiers TREK‐1 and TOK1, which pass outward K+ current with an activation potential that is coupled to the equilibrium potential for potassium, showed the strongest inhibition by bupivacaine. TWIK‐1 currents expressed in oocytes were small, but both inward and outward currents were diminished by bupivacaine.
We studied three local anesthetics without a piperidine group (lidocaine, etidocaine, and tetracaine) and four with a piperidine group (mepivacaine, R[+]‐ropivacaine, S[‐]‐ropivacaine, and bupivacaine) to determine whether octanol:buffer distribution coefficients (Q) of specific local anesthetics correlate with their potency to inhibit TASK currents. Q is defined as the ratio at equilibrium of the concentration of local anesthetic in an organic phase at a certain pH to the concentration in an aqueous phase and is used to quantify the degree of hydrophobic partitioning at that pH. [21] Q varies with pH for a given local anesthetic because protonation of the local anesthetic molecules makes them less likely to partition into octanol. Several direct correlations between local anesthetic potency and hydrophobicity have been reported previously. [33]
All local anesthetics inhibited TASK currents in a dose‐dependent manner. The Hill coefficients for all local anesthetics were close to unity, suggesting that one local anesthetic molecule binds to each TASK channel, presumably constituted as a dimer of two subunits. Because local anesthetic inhibition of TASK was slightly greater at depolarized potentials, local anesthetics may gain access to the TASK channel from the inside of the cell, as has been described for the hKv1.5 channel. [34] The Woodhull coefficients of local anesthetic inhibition of TASK currents were small, suggesting a relatively peripheral site of local anesthetic action. The approximate IC50 of lidocaine on TASK (222 [micro sign]M) is between the reported affinity for the resting state (1.4 mM) and the inactivated state (11 [micro sign]M) of voltage‐gated Na+ channels. [35] With the exception of tetracaine, which showed a much weaker block than predicted, there was a good correlation between local anesthetic Q values and their inhibition of TASK currents (R2 = 0.78, P < 0.05). The product of Q and the IC50 of tetracaine was markedly elevated compared with other local anesthetics, implying resistance to this local anesthetic (Table 1). Although hydrophobic sites are important determinants of local anesthetic inhibition of TASK currents, resistance to tetracaine implies that structural elements of local anesthetics also can contribute to the potency of inhibition.
The relative insensitivity of the ester‐linked tetracaine to the TASK channel may result from unknown agent‐specific characteristics. Interestingly, the flicker channel also has been reported to be resistant to inhibition by tetracaine and other ester‐linked local anesthetics. [9] However, the flicker channel also showed a marked difference in the potency of local anesthetics with and without a piperidine group, which we did not observe for the TASK currents.
In contrast to baseline K+ channels, other ion channels have not been reported to be resistant to ester‐linked anesthetics. Ester‐linked local anesthetics, for a given distribution coefficient or partition coefficient of the neutral species, are more potent inhibitors of resting Na (+) channels and compound action potentials. [21,33,36]
Back to Top | Article Outline
pH Dependence of Local Anesthetic Inhibition of Tandem Pore Domain K+ Channels
pH affects the distribution of local anesthetics between the aqueous phase and the lipid membrane, and it also influences the charge of membrane‐associated local anesthetics. Lower cytoplasmic pH favors the protonated form of tertiary amine local anesthetics, thereby increasing their potency of inhibition of voltage‐gated Na+ channels. [37] In contrast, decreasing the external pH will lead to a slower penetration because the neutral species penetrates the membrane more effectively. [37] The inhibition of TOK1 currents by bupivacaine was significantly greater at pH 7.6 than at pH 6.4. The inhibition of TASK currents also was considerably greater at higher pH. Interestingly, a similar pH dependence has been shown for the inhibition of the flicker channel by bupivacaine. [9] Our findings suggest that the uncharged form of bupivacaine may be important for the inhibition of TOK1 and TASK currents. This suggestion is also supported by the observation that QX314, a permanently charged quartenary lidocaine derivative, which is therefore relatively lipid insoluble, has no effect on TASK currents. [10] In addition, benzocaine, a permanently neutral local anesthetic, can inhibit TASK currents. Inhibition of TOK1 and TASK currents at different pH levels correlated with the Q values (calculated according to Equation 1 in Materials and Methods) at the corresponding pH (QpH8.4 = 1,559, QpH7.6 = 507, QpH7.0 = 150, QpH6.4 = 41).
Back to Top | Article Outline
There is increasing interest in the stereospecific effects and toxicity of local anesthetics, particularly of bupivacaine and ropivacaine. [18‐20,38] This interest derives from the relatively high clinical potency and low cardiac toxicity profile of the S(‐)‐stereoisomers of bupivacaine and ropivacaine compared with their R(+)‐stereoisomers. [39] In isolated frog peripheral nerve, stereoselective effects of bupivacaine on neuronal Na+ channels for the tonic and phasic inhibition have been reported. For both these effects, the R(+)‐enantiomer was found to be more potent, with stereopotency ratios (+:‐) of 1:2 and 2.5:3, respectively. [18]
Some known K+ channels also display a stereoselective local anesthetic block. [20,40] However, an outwardly rectifying K+ channel, expressed in the rat heart, has been reported to be equally sensitive to the R(+)‐ and S(‐)‐ stereoisomers of local anesthetics. [4] We also did not find a statistically significant difference between the R(+)‐ and S(‐)‐stereoisomers of ropivacaine at three different concentrations with respect to TASK inhibition. The lack of a steroselective block clearly distinguishes the TASK channel expressed in oocytes from the flicker channel in myelinated nerve, which shows a stereopotency ratio (+:‐) of 20 for ropivacaine and 67 for bupivacaine. [40]
The potency of R(+)‐bupivacaine on a K+ channel cloned from human ventricle (hKv1.5) is approximately seven times greater than that exhibited by S(‐)‐bupivacaine. [20] A recent report of the molecular determinants of this stereoselective block shows that stereoselectivity required interaction with amino acid residues contained in the sixth transmembrane domain (threonine 505, leucine 508, and valine 512) of hKv1.5. [41] Major structural differences between tandem pore domain and voltage‐gated K+ channels prevent accurate alignments of the protein sequences of TASK and hKv1.5. However, the hKv1.5 results suggest that site‐directed mutagenesis of TASK within the postpore transmembrane domains might increase the stereoselectivity of TASK local anesthetic inhibition.
Back to Top | Article Outline
Depolarization of the Resting Membrane Potential
Historically, in a wide variety of experimental paradigms, local anesthetics have been reported to slow the rate of rise of compound action potentials rather than to affect the resting membrane potential. [42] However, in frog sciatic nerves, the addition of the K+ channel blockers tetraethylammonium ion or 3,4‐diaminopyridine increased lidocaine‐induced tonic and phasic inhibition by 15 and 28%, respectively. [8] Similar results were achieved for the phasic inhibition by bupivacaine in the presence of the tetraethylammonium ion. The K+ channel blockers alone depolarized the resting membrane potential by 4‐6 mV. Local anesthetics preferentially bind to open and inactivated states of Na+ channels that predominate at depolarized membrane potentials. [43] Thus, K+ channel blockers, by causing partial depolarization and Na+ channel opening and inactivation, can increase the binding of local anesthetic to neuronal Na+ channels. [8] In addition, voltage‐clamp studies of cells expressing Na (+) channels also have documented the potentiating effect of membrane depolarization on local anesthetic inhibition of Na+ currents. [35]
The membrane potentials of TASK cRNA‐injected oocytes were depolarized by 20‐30 mV by the application of 1 mM local anesthetic. When saline‐injected control oocytes were hyperpolarized by constant current injection, bupivacaine still depolarized the membrane potential only by approximately 7 mV. This finding suggests that the local anesthetic‐induced depolarization of TASK cRNA‐injected oocytes does not depend on the relatively negative resting membrane potential of cRNA‐injected oocytes.
Studies have shown that the resting membrane potential of several cell types is controlled by potassium channels that are not voltage gated. [44,45] The inhibition of baseline tandem pore domain K+ channels by local anesthetics could depolarize the resting membrane potential and augment the conduction block of peripheral nerves by promoting the formation of open and inactivated states of voltage‐gated Na+ channels, which are highly sensitive to local anesthetics.
Back to Top | Article Outline


The results of our study indicate that (1) the tandem pore domain K+ channels TASK, TREK‐1, TOK1, ORK1, and TWIK‐1 are inhibited by bupivacaine; (2) bupivacaine inhibition of TOK1 and TASK is pH dependent, and the presence of the uncharged form of the local anesthetic is important for inhibition; (3) the local anesthetic inhibition of TASK currents is dose dependent, and the potency of the block by different local anesthetics is predicted by their octanol:buffer distribution coefficients, with the exception of tetracaine, to which TASK is relatively insensitive; (4) the R(+)‐ and S(‐)‐stereoisomers of ropivacaine were equipotent with respect to the inhibition of TASK currents and, therefore, were not stereoselective; and (5) the local anesthetic inhibition of TASK currents depolarizes the resting membrane potential of cells expressing this channel, thereby possibly contributing to conduction block.
Future studies, such as mutagenesis experiments of existing local anesthetic‐sensitive baseline K+ channels, the identification of new channels such as these, and the determination of their expression by sensory nerves will improve our understanding of local anesthetic action.
The authors thank Dr. Steven Goldstein for his gift of the expression plasmids for TOK1 and ORK1, Dr. Michel Lazdunski for human TWIK‐1, Dr. Douglas Bayliss for mouse TREK‐1, and Winifred von Ehrenburg for editorial assistance.
Back to Top | Article Outline


1. Ragsdale DS, McPhee JC, Scheuer T, Catterall WA: Molecular determinants of state-dependent block of Na+ channels by local anesthetics. Science 1994; 265:1724-8

2. Carmeliet E, Morad M, Van der Heyden G, Vereecke J: Electrophysiological effects of tetracaine in single guinea-pig ventricular myocytes. J Physiol 1986; 376:143-61

3. Courtney KR, Kendig JJ: Bupivacaine is an effective potassium channel blocker in heart. Biochim Biophys Acta 1988; 939:163-6

4. Castle NA: Bupivacaine inhibits the transient outward K+ current but not the inward rectifier in rat ventricular myocytes. J Pharmacol Exp Ther 1990; 255:1038-46

5. Escande D, Coulombe A, Faivre JF, Deroubaix E, Coraboeuf E: Two types of transient outward currents in adult human atrial cells. Am J Physiol 1987; 252:H142-8

6. Kotelko DM, Shnider SM, Dailey PA, Brizgys RV, Levinson G, Shapiro WA, Koike M, Rosen MA: Bupivacaine-induced cardiac arrhythmias in sheep. Anesthesiology 1984; 60:10-8

7. Tucker GT, Mather LE: Pharmacology of local anaesthetic agents. Pharmacokinetics of local anaesthetic agents. Br J Anaesth 1975; 47:213-24

8. Drachman D, Strichartz G: Potassium channel blockers potentiate impulse inhibition by local anesthetics. Anesthesiology 1991; 75:1051-61

9. Brau ME, Nau C, Hempelmann G, Vogel W: Local anesthetics potently block a potential insensitive potassium channel in myelinated nerve. J Gen Physiol 1995; 105:485-505

10. Leonoudakis D, Gray AT, Winegar BD, Kindler CH, Harada M, Taylor DM, Chavez RA, Forsayeth JR, Yost CS: An open rectifier potassium channel with two pore domains in tandem cloned from rat cerebellum. J Neurosci 1998; 18:868-77

11. Duprat F, Lesage F, Fink M, Reyes R, Heurteaux C, Lazdunski M: TASK, a human background K+ channel to sense external pH variations near physiological pH. EMBO J 1997; 16:5464-71

12. Lesage F, Guillemare E, Fink M, Duprat F, Lazdunski M, Romey G, Barhanin J: TWIK-1, a ubiquitous human weakly inward rectifying K+ channel with a novel structure. EMBO J 1996; 15:1004-11

13. Winegar BD, Owen DF, Yost CS, Forsayeth JR, Mayeri E: Volatile general anesthetics produce hyperpolarization of Aplysia neurons by activation of a discrete population of baseline potassium channels. Anesthesiology 1996; 85:889-900

14. Lesage F, Reyes R, Fink M, Duprat F, Guillemare E, Lazdunski M: Dimerization of TWIK-1 K+ channel subunits via a disulfide bridge. EMBO J 1996; 15:6400-7

15. Ketchum KA, Joiner WJ, Sellers AJ, Kaczmarek LK, Goldstein SA: A new family of outwardly rectifying potassium channel proteins with two pore domains in tandem. Nature 1995; 376:690-5

16. Goldstein SA, Price LA, Rosenthal DN, Pausch MH: ORK1, a potassium-selective leak channel with two pore domains cloned from Drosophila melanogaster by expression in Saccharomyces cerevisiae. Proc Natl Acad Sci 1996; 93:13256-61

17. Fink M, Duprat F, Lesage F, Reyes R, Romey G, Heurteaux C, Lazdunski M: Cloning, functional expression and brain localization of a novel unconventional outward rectifier K+ channel. EMBO J 1996; 15:6854-62

18. Lee-Son S, Wang GK, Concus A, Crill E, Strichartz G: Stereoselective inhibition of neuronal sodium channels by local anesthetics. Evidence for two sites of action? Anesthesiology 1992; 77:324-35

19. Valenzuela C, Snyders DJ, Bennett PB, Tamargo J, Hondeghem LM: Stereoselective block of cardiac sodium channels by bupivacaine in guinea pig ventricular myocytes. Circulation 1995; 92:3014-24

20. Valenzuela C, Delpon E, Tamkun MM, Tamargo J, Snyders DJ: Stereoselective block of a human cardiac potassium channel (Kv1.5) by bupivacaine enantiomers. Biophys J 1995; 69:418-27

21. Strichartz GR, Sanchez V, Arthur GR, Chafetz R, Martin D: Fundamental properties of local anesthetics. II. Measured octanol: buffer partition coefficients and pKa values of clinically used drugs. Anesth Analg 1990; 71:158-70

22. Woodhull AM: Ionic blockage of sodium channels in nerve. J Gen Physiol 1973; 61:687-708

23. Lesage F, Guillemare E, Fink M, Duprat F, Lazdunski M, Romey G, Barhanin J: A pH-sensitive yeast outward rectifier K+ channel with two pore domains and novel gating properties. J Biol Chem 1996; 271:4183-7

24. Stuhmer W, Parekh AB: Electrophysiological recordings from Xenopus oocytes, Single-Channel Recording, 2nd ed. Edited by B Sakmann, E Neher. New York, Plenum Press, 1995, pp 341-56

25. Lesage F, Lauritzen I, Duprat F, Reyes R, Fink M. Heurteaux C, Lazdunski M: The structure, function and distribution of the mouse TWIK-1 K+ channel. FEBS Lett 1997; 402:28-32

26. Kim D, Fujita A, Horio Y, Kurachi Y: Cloning and functional expression of a novel cardiac two-pore background K+ channel (cT-BAK-1). Circ Res 1998; 82:513-18

27. Fink M, Lesage F, Duprat F, Heurteaux C, Reyes R, Fosset M, Lazdunski M: A neuronal two P domain K+ channel stimulated by arachidonic acid and polyunsaturated fatty acids. EMBO J 1998; 17:3297-308

28. Koh DS, Jonas P, Brau ME, Vogel W: A TEA-insensitive flickering potassium channel active around the resting potential in myelinated nerve. J Membr Biol 1992; 130:149-62

29. Koh DS, Jonas P, Vogel W: Na+ -activated K+ channels localized in the nodal region of myelinated axons of Xenopus. J Physiol 1994; 479:183-97

30. Koyano K, Tanaka K, Kuba K: A patch-clamp study on the muscarine-sensitive potassium channel in bullfrog sympathetic ganglion cells. J Physiol 1992; 454:231-46

31. Rae JL, Dewey J, Cooper K: Properties of single potassium-selective ionic channels from the apical membrane of rabbit corneal endothelium. Exp Eye Res 1989; 49:591-609

32. Schmid A, Schulz I: Characterization of single potassium channels in mouse pancreatic acinar cells. J Physiol 1995; 484:661-76

33. Courtney KR: Structure-activity relations for frequency-dependent sodium channel block in nerve by local anesthetics. J Pharmacol Exp Ther 1980; 213:114-9

34. Valenzuela C, Delpon E, Franqueza L, Gay P, Snyders DJ, Tamargo J: Effects of ropivacaine on a potassium channel (hKv1.5) cloned from human ventricle. Anesthesiology 1997; 86:718-28

35. Ragsdale DS, McPhee JC, Scheuer T, Catterall WA: Common molecular determinants of local anesthetic, antiarrhythmic, and anti-convulsant block of voltage-gated Na+ channels. Proc Natl Acad Sci 1996; 93:9270-5

36. Wildsmith JA, Gissen AJ, Takman B, Covino BG: Differential nerve blockade: Esters v. amides and the influence of pKa. Br J Anaesth 1987; 59:379-84

37. Butterworth JF, Strichartz GR: Molecular mechanisms of local anesthesia: A review. Anesthesiology 1990; 72:711-34

38. Graf BM, Martin E, Bosnjak ZJ, Stowe DF: Stereospecific effect of bupivacaine isomers on atrioventricular conduction in the isolated perfused guinea pig heart. Anesthesiology 1997; 86:410-9

39. Calvey TN: Chirality in anaesthesia (editorial). Anaesthesia 1992; 47:93-4

40. Nau C, Brau ME, Hempelmann G, Vogel W: Structure-activity-relationship and stereoselectivity of local anesthetic homologues in a potassium channel of myelinated nerve. Presented at: The Fifth International Conference on Molecular and Cellular Mechanisms of Anesthesia; June 18-20, 1997; Calgary, Alberta, Canada, p 39

41. Franqueza L, Longobardo M, Vicente J, Delpon E, Tamkun MM, Tamargo J, Snyders DJ, Valenzuela C: Molecular determinants of stereoselective bupivacaine block of hKv1.5 channels. Circ Res 1997; 81:1053-64

42. Shanes AM, Freygang WH, Grundfest H, Amatniek E: Anesthetic and calcium action in the voltage clamped squid giant axon. J Gen Physiol 1959; 42:793-802

43. Hille B, Courtney K, Dum R: Rate and site of local anesthetics in myelinated nerve fibers, Progress in Anesthesiology, vol. 1, Molecular Mechanisms of Anesthesia. Edited by BR Fink. New York, Raven Press, 1975, pp 13-20

44. Chang DC: Is the K permeability of the resting membrane controlled by the excitable K channel? Biophys J 1986; 50:1095-100

45. Jones SW: On the resting potential of isolated frog sympathetic neurons. Neuron 1989; 3:153-61

Cited By:

This article has been cited 65 time(s).

Journal of Physiology-London
A pH-sensitive potassium conductance (TASK) and its function in the murine gastrointestinal tract
Cho, SY; Beckett, EA; Baker, SA; Han, I; Park, KJ; Monaghan, K; Ward, SM; Sanders, KM; Koh, SD
Journal of Physiology-London, 565(1): 243-259.
Journal of Neuroscience
TWIK-1 and TREK-1 Are Potassium Channels Contributing Significantly to Astrocyte Passive Conductance in Rat Hippocampal Slices
Zhou, M; Xu, GJ; Xie, MJ; Zhang, XX; Schools, GP; Ma, LQ; Kimelberg, HK; Chen, HJ
Journal of Neuroscience, 29(): 8551-8564.
Trends in Pharmacological Sciences
Ions in the fire: recent ion-channel research and approaches to pain therapy
Eglen, RM; Hunter, JC; Dray, A
Trends in Pharmacological Sciences, 20(8): 337-342.

Nature Reviews Neuroscience
Potassium leak channels and the KCNK family of two-P-domain subunits
Goldstein, SAN; Bockenhauer, D; O'Kelly, I; Zilberberg, N
Nature Reviews Neuroscience, 2(3): 175-184.

Neuroscience Research
Bupivacaine, but not tetracaine, protects against the in vitro ischemic insult of rat hippocampal CA1 neurons
Niiyama, S; Tanaka, E; Yamamoto, S; Yasumoto, S; Kano, T; Higashi, H
Neuroscience Research, 42(3): 231-241.
PII S0168-0102(01)00326-1
Anesthesia and Analgesia
Inhibition of human TREK-1 channels by bupivacaine
Punke, MA; Licher, T; Pongs, O; Friederich, P
Anesthesia and Analgesia, 96(6): 1665-1673.
Trends in Neurosciences
The TASK background K-2P channels: chemo- and nutrient sensors
Duprat, F; Lauritzen, I; Patel, A; Honore, E
Trends in Neurosciences, 30(): 573-580.
Naunyn-Schmiedebergs Archives of Pharmacology
The human cardiac K(2P)3.1 (TASK-1) potassium leak channel is a molecular target for the class III antiarrhythmic drug amiodarone
Gierten, J; Ficker, E; Bloehs, R; Schweizer, PA; Zitron, E; Scholz, E; Karle, C; Katus, HA; Thomas, D
Naunyn-Schmiedebergs Archives of Pharmacology, 381(3): 261-270.
Forward transport: 14-3-3 binding overcomes retention in endoplasmic reticulum by dibasic signals
O'Kelly, I; Butler, MH; Zilberberg, N; Goldstein, SAN
Cell, 111(4): 577-588.

Pharmacology of neuronal background potassium channels
Lesage, F
Neuropharmacology, 44(1): 1-7.
Journal of Neuroscience
Contribution of TWIK-related acid-sensitive K+ channel 1 (TASK1) and TASK3 channels to the control of activity modes in thalamocortical neurons
Meuth, SG; Budde, T; Kanyshkova, T; Broicher, T; Munsch, T; Pape, HC
Journal of Neuroscience, 23(): 6460-6469.

British Journal of Pharmacology
Functional evidence of a role for two-pore domain potassium channels in rat mesenteric and pulmonary arteries
Gardener, MJ; Johnson, IT; Burnham, MP; Edwards, G; Heagerty, AM; Weston, AH
British Journal of Pharmacology, 142(1): 192-202.
Pflugers Archiv-European Journal of Physiology
Two-pore-domain potassium channels support anion secretion from human airway Calu-3 epithelial cells
Davis, KA; Cowley, EA
Pflugers Archiv-European Journal of Physiology, 451(5): 631-641.
Journal of Membrane Biology
Biophysical and pharmacological characteristics of native two-pore domain TASK channels in rat adrenal glomerulosa cells
Lotshaw, DP
Journal of Membrane Biology, 210(1): 51-70.
Journal of Neuroscience
Mutants of a temperature-sensitive two-P domain potassium channel
Kunkel, MT; Johnstone, DB; Thomas, JH; Salkoff, L
Journal of Neuroscience, 20(): 7517-7524.

Proceedings of the National Academy of Sciences of the United States of America
The temperature-signaling cascade in sponges involves a heat-gated cation channel, abscisic acid, and cyclic ADP-ribose
Zocchi, E; Carpaneto, A; Cerrano, C; Bavestrello, G; Giovine, M; Bruzzone, S; Guida, L; Franco, L; Usai, C
Proceedings of the National Academy of Sciences of the United States of America, 98(): 14859-14864.

Journal of General Physiology
The effects of polarizing current on nerve terminal impulses recorded from polymodal and cold receptors in the guinea-pig cornea
Carr, RW; Pianova, S; Brock, JA
Journal of General Physiology, 120(3): 395-405.
Journal of Neuroscience
Transcriptional and Electrophysiological Maturation of Neocortical Fast-Spiking GABAergic Interneurons
Okaty, BW; Miller, MN; Sugino, K; Hempel, CM; Nelson, SB
Journal of Neuroscience, 29(): 7040-7052.
British Journal of Pharmacology
Local anaesthetics block hyperpolarization-activated inward current in rat small dorsal root ganglion neurones
Bischoff, U; Brau, ME; Vogel, W; Hempelmann, G; Olschewski, A
British Journal of Pharmacology, 139(7): 1273-1280.
Pflugers Archiv-European Journal of Physiology
The 2P-domain K+ channels: role in apoptosis and tumorigenesis
Patel, AJ; Lazdunski, M
Pflugers Archiv-European Journal of Physiology, 448(3): 261-273.
Molecular Pharmacology
Serotonin inhibits neuronal excitability by activating two-pore domain K+ channels in the entorhinal cortex
Deng, PY; Poudel, KS; Rojanathammanee, L; Porter, JE; Lei, S
Molecular Pharmacology, 72(1): 208-218.
Biologicheskie Membrany
Tandem-pore domain and inwardly rectifying potassium channels: K+ buffering by retinal (Muller) glial cells and cortical astrocytes
Skatchkov, SN; Kucheryavykh, YV; Antonov, SM; Pearson, WL; Nichois, CG; Reichenbach, A; Eaton, MJ
Biologicheskie Membrany, 23(2): 85-100.

Journal of Physiology-London
An oxygen-, acid- and anaesthetic-sensitive TASK-like background potassium channel in rat arterial chemoreceptor cells
Buckler, KJ; Williams, BA; Honore, E
Journal of Physiology-London, 525(1): 135-142.

Journal of Cell Science
ABA- and cADPR-mediated effects on respiration and filtration downstream of the temperature-signaling cascade in sponges
Zocchi, E; Basile, G; Cerrano, C; Bavestrello, G; Giovine, M; Bruzzone, S; Guida, L; Carpaneto, A; Magrassi, R; Usai, C
Journal of Cell Science, 116(4): 629-636.
Current Opinion in Investigational Drugs
Therapeutic potential of neuronal two-pore domain potassium-channel modulators
Mathie, A; Veale, EL
Current Opinion in Investigational Drugs, 8(7): 555-562.

Bju International
Functional and molecular identification of pH-sensitive K+ channels in murine urinary bladder smooth muscle
Beckett, EAH; Han, I; Baker, SA; Han, J; Britton, FC; Koh, SD
Bju International, 102(1): 113-124.
Acid-Sensitive Task-Like K+ Conductances Contribute to Resting Membrane Potential and to Orexin-Induced Membrane Depolarization in Rat Thalamic Paraventricular Nucleus Neurons
Doroshenko, P; Renaud, LP
Neuroscience, 158(4): 1560-1570.
Two-pore-domain (KCNK) potassium channels: Dynamic roles in neuronal function
Talley, EM; Sirois, JE; Lei, QB; Bayliss, DA
Neuroscientist, 9(1): 46-56.
Molecular Brain Research
Identification of native rat cerebellar granule cell currents due to background K channel KCNK5 (TASK-2)
Cotten, JF; Zou, HL; Liu, CH; Au, JD; Yost, CS
Molecular Brain Research, 128(2): 112-120.
Journal of Cellular Physiology
Expression of the mechanosensitive 2PK+channel TREK-1 in human osteoblasts
Hughes, S; Magnay, J; Foreman, M; Publicover, SJ; Dobson, OP; El Haj, AJ
Journal of Cellular Physiology, 206(3): 738-748.
Neuroscience Letters
A novel acid-sensitive K+ channel in rat dorsal root ganglia neurons
La, JH; Kang, DW; Park, JY; Hong, SG; Han, JH
Neuroscience Letters, 406(3): 244-249.
Current Pharmaceutical Design
Physiology and pharmacology of two-pore domain potassium channels
Kim, D
Current Pharmaceutical Design, 11(): 2717-2736.

Alternative translation initiation in rat brain yields K(2P)2.1 potassium channels permeable to sodium
Thomas, D; Plant, LD; Wilkens, CM; McCrossan, ZA; Goldstein, SAN
Neuron, 58(6): 859-870.
Functional characterisation of human TASK-3, an acid-sensitive two-pore domain potassium channel
Meadows, HJ; Randall, AD
Neuropharmacology, 40(4): 551-559.

American Journal of Physiology-Cell Physiology
Differential effects of volatile and intravenous anesthetics on the activity of human TASK-1
Putzke, C; Hanley, PJ; Schlichthorl, G; Preisig-Muller, R; Rinne, S; Anetseder, M; Eckenhoff, R; Berkowitz, C; Vassiliou, T; Wulf, H; Eberhart, L
American Journal of Physiology-Cell Physiology, 293(4): C1319-C1326.
Anesthesia and Analgesia
Species-specific differences in response to anesthetics and other modulators by the K-2P channel TRESK
Keshavaprasad, B; Liu, CH; Au, JD; Kindler, CH; Cotten, JF; Yost, CS
Anesthesia and Analgesia, 101(4): 1042-1049.
Journal of Physiology-London
Effects of divalent cations and spermine on the K+ channel TASK-3 and on the outward current in thalamic neurons
Musset, B; Meuth, SG; Liu, GX; Derst, C; Wegner, S; Pape, HC; Budde, T; Preisig-Muller, R; Daut, J
Journal of Physiology-London, 572(3): 639-657.
Expression of TWIK-related acid sensitive K+ channels in capsaicin sensitive and insensitive cells of rat dorsal root ganglia
Rau, KK; Cooper, BY; Johnson, RD
Neuroscience, 141(2): 955-963.
Journal of Physiology-London
Temperature-sensitive TREK currents contribute to setting the resting membrane potential in embryonic atrial myocytes
Zhang, H; Shepherd, N; Creazzo, TL
Journal of Physiology-London, 586(): 3645-3656.
Molecular and Cellular Neuroscience
Serotonin increases GABA release in rat entorhinal cortex by inhibiting interneuron TASK-3 K+ channels
Deng, PY; Lei, SB
Molecular and Cellular Neuroscience, 39(2): 273-284.
Biochimica Et Biophysica Acta-Biomembranes
Two-pore domain K+ channels - molecular sensors
O'Connell, AD; Morton, MJ; Hunter, M
Biochimica Et Biophysica Acta-Biomembranes, 1566(): 152-161.
PII S0005-2736(02)00597-70
Molecular Pharmacology
The contribution of TWIK-related acid-sensitive K+-containing channels to the function of dorsal lateral geniculate thalamocortical relay neurons
Meuth, SG; Aller, MI; Munsch, T; Schuhmacher, T; Seidenbecher, T; Meuth, P; Kleinschnitz, C; Pape, HC; Wiendl, H; Wisden, W; Budde, T
Molecular Pharmacology, 69(4): 1468-1476.
Regional Anesthesia and Pain Medicine
The Effect of Levosimendan on Bupivacaine-Induced Severe Myocardial Depression in Anesthetized Pigs
Aittomaki, J; Liuhanen, S; Sallisalmi, M; Salmenpera, MT; Heavner, JE; Rosenberg, PH
Regional Anesthesia and Pain Medicine, 35(1): 34-40.
Anesthesia and Analgesia
Patch-clamp analysis of anesthetic interactions with recombinant SK2 subtype neuronal calcium-activated potassium channels
Dreixler, JC; Jenkins, A; Cao, YJ; Roizen, JD; Houamed, KM
Anesthesia and Analgesia, 90(3): 727-732.

Anasthesiologie Intensivmedizin Notfallmedizin Schmerztherapie
Two-pore-domain potassium channels
Brau, M
Anasthesiologie Intensivmedizin Notfallmedizin Schmerztherapie, 35(9): 595-596.

Journal of Pharmacology and Experimental Therapeutics
Amide local anesthetics potently inhibit the human tandem pore domain background K+ channel TASK-2 (KCNK5)
Kindler, CH; Paul, M; Zou, H; Liu, CH; Winegar, BD; Gray, AT; Yost, CS
Journal of Pharmacology and Experimental Therapeutics, 306(1): 84-92.
Regional Anesthesia and Pain Medicine
Two-pore domain potassium channels: New sites of local anesthetic action and toxicity
Kindler, CH; Yost, CS
Regional Anesthesia and Pain Medicine, 30(3): 260-274.
Tandem-pore domain potassium channels are functionally expressed in retinal (Muller) glial cells
Skatchkov, SN; Eaton, MJ; Shuba, YM; Kucheryavykh, YV; Derst, C; Veh, RW; Wurm, A; Iandiev, I; Pannicke, T; Bringman, A; Reichenbach, A
Glia, 53(3): 266-276.
American Journal of Physiology-Cell Physiology
VOCCs and TREK-1 ion channel expression in human tenocytes
Magra, M; Hughes, S; El Haj, AJ; Maffulli, N
American Journal of Physiology-Cell Physiology, 292(3): C1053-C1060.
Forward transport of K(2P)3.1: Mediation by 14-3-3 and COPI, modulation by p11
O'Kelly, I; Goldstein, SAN
Traffic, 9(1): 72-78.
Physiological Reviews
Molecular Background of Leak K+ Currents: Two-Pore Domain Potassium Channels
Enyedi, P; Czirjak, G
Physiological Reviews, 90(2): 559-605.
Journal of Biological Chemistry
TASK-3, a novel tandem pore domain acid-sensitive K+ channel - An extracellular histidine as pH sensor
Rajan, S; Wischmeyer, E; Liu, GX; Muller, RP; Daut, J; Karschin, A; Derst, C
Journal of Biological Chemistry, 275(): 16650-16657.

British Journal of Pharmacology
Enhancement of delayed-rectifier potassium conductance by low concentrations of local anaesthetics in spinal sensory neurones
Olschewski, A; Wolff, M; Brau, ME; Hempelmann, G; Vogel, W; Safronov, BV
British Journal of Pharmacology, 136(4): 540-549.

Anesthesia and Analgesia
Potent activation of the human tandem pore domain K channel TRESK with clinical concentrations of volatile anesthetics
Liu, CH; Au, JD; Zou, HL; Cotten, JF; Yost, CS
Anesthesia and Analgesia, 99(6): 1715-1722.
Brain Research
Two-pore-domain potassium channels contribute to neuronal potassium release and glial potassium buffering in the rat hippocampus
Pasler, D; Gabriel, S; Heinemann, U
Brain Research, 1173(): 14-26.
Anesthesia and Analgesia
Mexiletine and Lidocaine Suppress the Excitability of Dorsal Horn Neurons
Olschewski, A; Schnoebel-Ehehalt, R; Li, YJ; Tang, B; Brau, ME; Wolff, M
Anesthesia and Analgesia, 109(1): 258-264.
Chemoreception: From Cellular Signalling to Functional Plasticity
2P domain K+ channels : Novel pharmacological targets for volatile general anesthetics
Patel, AJ; Honore, E
Chemoreception: From Cellular Signalling to Functional Plasticity, 536(): 9-23.

Anesthesia and Analgesia
Enantioselective actions of bupivacaine and ropivacaine on coronary vascular resistance at cardiotoxic concentrations
Burmester, MD; Schluter, KD; Daut, J; Hanley, PJ
Anesthesia and Analgesia, 100(3): 707-712.
Evidence that task1 channels contribute to the background current in Ah/type 11 neurons of the guinea-pig intestine
Matsuyama, H; Nguyen, TV; Hunne, B; Thacker, M; Needham, K; McHugh, D; Furness, JB
Neuroscience, 155(3): 738-750.
Canadian Journal of Physiology and Pharmacology
Volume regulation in the human airway epithelial cell line Calu-3
Harron, SA; Clarke, CM; Jones, CL; Babin-Muise, D; Cowley, EA
Canadian Journal of Physiology and Pharmacology, 87(5): 337-346.
American Journal of Physiology-Renal Physiology
Molecular and functional properties of two-pore-domain potassium channels
Lesage, F; Lazdunski, M
American Journal of Physiology-Renal Physiology, 279(5): F793-F801.

Cell Biochemistry and Biophysics
Biophysical, pharmacological, functional characteristics of cloned and native mammalian two-pore domain K+ channels
Lotshaw, DP
Cell Biochemistry and Biophysics, 47(2): 209-256.
Journal of Pharmacology and Experimental Therapeutics
TASK-3 knockout mice exhibit exaggerated nocturnal activity, impairments in cognitive functions, and reduced sensitivity to inhalation Anesthetics
Linden, AM; Sandu, C; Aller, MI; Vekovischeva, OY; Rosenberg, PH; Wisden, W; Korpi, ER
Journal of Pharmacology and Experimental Therapeutics, 323(3): 924-934.
Anesthesia and Analgesia
Bupivacaine suppresses [Ca2+](i) oscillations in neonatal rat cardiomyocytes with increased extracellular K+ and is reversed with increased extracellular Mg2+
McCaslin, PP; Butterworth, J
Anesthesia and Analgesia, 91(1): 82-88.

Anesthetic-sensitive 2P Domain K+ Channels
Patel, AJ; Honoré, E
Anesthesiology, 95(4): 1013-1021.

PDF (1102)
Back to Top | Article Outline
Baseline conductance; stereoselectivity; voltage‐clamp; Xenopus oocytes.

© 1999 American Society of Anesthesiologists, Inc.

Publication of an advertisement in Anesthesiology Online does not constitute endorsement by the American Society of Anesthesiologists, Inc. or Lippincott Williams & Wilkins, Inc. of the product or service being advertised.

Article Tools



Search for Similar Articles
You may search for similar articles that contain these same keywords or you may modify the keyword list to augment your search.