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Metabolism of Halothane in Obese Fischer 344 Rats.

Blermann, J. Sybil M.D.; Rice, Susan A. Ph.D.; Fish, Kevin J. M.B., Ch.B.; Serra, Mauricio T. M.S.

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Halothane is metabolized by an oxidative pathway to stable, nonvolatile end products, trifluoroacetic acid (TFAA) and bromide (Br-), and by reductive pathways to Br- and inorganic fluoride (F-). There is evidence that both oxidatively and reductively formed intermediates may produce hepatotoxicity, although the exact etiology of the fulminant hepatic necrosis seen in humans is unproven. Obese patients receiving volatile anesthetics exhibit higher serum anesthetic metabolite concentrations than do normal-weight patients, and thus might be at greater risk of hepatotoxicity because of higher concentrations of reactive intermediates from halothane metabolism. To eliminate the variables inherent in human clinical studies leading to confounding interpretation of data, this study determined the contributions of oxidative and reductive pathways to halothane metabolism in an animal model of human hypertrophic obesity, the most common form of human obesity. Eight pairs of obese (highfat diet) and normal-weight (standard chow), male Fischer 344 rats were anesthetized with halothane for 4 h at an inspired concentration of 0.78%. Serum and urinary concentrations of TFAA, Br-, and F- were measured. Thirty-six hours following halothane anesthesia, mean serum TFAA concentrations peaked at 7.3 +/- 1.1 mM in obese rats and 4.7 +/- 0.7 mM in nonobese rats. TFAA urinary excretions during the 180-h period postanesthesia were 519 +/- 69 and 336 +/- 22 [mu]mol, respectively. Peak serum Br- concentrations were 9.1 +/- 1.0 and 6.9 +/- 0.6 mM for obese and nonobese rats, respectively, and Br- urinary excretions were 127 +/- 30 and 79 +/- 14 [mu]mol, respectively. Peak serum F- concentrations of 4.1 +/- 1.0 and 4.9 +/- 0.6 [mu]M for obese and nonobese rats were not significantly different; F- excretions were not elevated. These data demonstrate that halothane metabolite production via the oxidative pathway is significantly increased in obese compared with nonobese rats. In contrast, there is no evidence for metabolite production via reductive routes in obese rats above that seen in nonobese rats.
(C) 1989 American Society of Anesthesiologists, Inc.
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