Abstract: Comparison of DNA profiles is often used in verifying the identification of deceased human beings when other easier, quicker, and less expensive means to identification are not possible. Fifty-five adult subjects divided into 3 groups provided a used toothbrush along with a small bloodstain control for DNA analysis and comparison. Results indicate that there is no significant difference in the quantity and quality of DNA recovered from a toothbrush that has been used for 1 month versus 3 months versus random periods. The results of this study confirm earlier conclusions that a used toothbrush is a reliable source of antemortem DNA from a putative decedent. The use of aviation snips to remove a small portion of the toothbrush head provides an easy, inexpensive method of obtaining a sample for DNA extraction. The authors recommend this method as a standardized technique for use in forensic DNA laboratories.