Aging of Wound Healing in an Experimental Model in Mice.Hiss, Jehuda M.D.; Hirshberg, Abraham M.D., D.M.D.; Dayan, Dan Fundoiano D.M.D., M.Sc.; Bubis, Jose J. M.D.; Wolman, Moshe M.D.American Journal of Forensic Medicine & Pathology: December 1988 Technical Note: PDF Only Abstract Healing of incised wounds in the backs of mice was studied to establish criteria that would allow the timing of stages during cutaneous wound healing. Starting from day 4 after the incisions were made, three mice were killed every 3 days until day 40. Sections from wounds were stained with hematoxylin-eosin, a reticulin procedure, and picrosirius red. Unstained sections were used for blue autofluorescence. The significance of the findings from the ultraviolet study was limited, but the picrosirius red-polarized light procedure was found to be useful for estimating the age of the repair process as polarization colors of greater wavelengths progressively increased with the age of the lesion (C) Lippincott-Raven Publishers.