Enter your Email address:
Wolters Kluwer Health may email you for journal alerts and information, but is committed
to maintaining your privacy and will not share your personal information without
You currently have no recent searches
Leclerc-Mercier, Stéphanie MD; Hovnanian, Alain MD, PhD; Fraitag, Sylvie MD
Pathology Department and MAGEC Center for Rare Cutaneous Diseases, Hopital Necker-Enfants Malades, APHP, Paris, France
The authors declare no conflicts of interest.
To the Editor:
In a recent article, Dadzie et al1 highlighted the advances in molecular diagnosis in dermatopathology. We read the article with great interest because they made a comprehensive review of the different molecular techniques, pointing out their role as an ancillary tool for the diagnosis of different cutaneous disorders.
Among the different clinical applications, they underlined the ichthyoses group. They stated that, “Netherton syndrome … can be confirmed with in situ hybridization on cutaneous tissue to demonstrate lack of expression of the serine protease inhibitor LEKTI,” quoting our article recently published on neonatal and infantile erythroderma.2
Lymphoepithelial Kazal type–related inhibitor (LEKTI) is indeed a serine protease inhibitor encoded by SPINK5 (serine protease inhibitor Kazal type 5), the causative gene of the severe autosomal recessive ichthyotic skin disorder, Netherton syndrome (NS).3,4 However, we use immunostaining of skin sections with a monoclonal antibody against D1–D6 domains of LEKTI provided by A. Hovnanian's research laboratory, to investigate LEKTI expression in NS patients.4 This antibody is used with a classical immunohistochemistry technique and a 3-step immunoperoxidase technique on formalin-fixed sections. It allows a rapid, reliable, and highly specific diagnosis of NS. Other groups use different LEKTI antibodies, but to the best of our knowledge in situ hybridization is not a technique commonly used for the diagnosis of NS.
© 2012 Lippincott Williams & Wilkins, Inc.
Colleague's E-mail is Invalid
Your Name: (optional)
Separate multiple e-mails with a (;).
Thought you might appreciate this item(s) I saw at The American Journal of Dermatopathology.
Send a copy to your email
Your message has been successfully sent to your colleague.
Some error has occurred while processing your request. Please try after some time.
An Existing Folder
A New Folder
The item(s) has been successfully added to "".
Login with your LWW Journals username and password.
Username or Email:
Enter and submit the email address you registered with. An email with instructions to reset your password will be sent to that address.
Link to reset your password has been sent to specified email address.
What does "Remember me" mean?
By checking this box, you'll stay logged in until you logout. You'll get easier access to your articles, collections,
media, and all your other content, even if you close your browser or shut down your
To protect your most sensitive data and activities (like changing your password),
we'll ask you to re-enter your password when you access these services.
What if I'm on a computer that I share with others?
If you're using a public computer or you share this computer with others, we recommend
that you uncheck the "Remember me" box.
Save my selection