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BRAF and KRAS Mutations in Sporadic Glomus Tumors

Chakrapani, Andrea MD; Warrick, Andrea BS; Nelson, Dylan BS; Beadling, Carol PhD; Corless, Christopher L. MD, PhD

American Journal of Dermatopathology:
doi: 10.1097/DAD.0b013e31823931b4
Brief Report
Abstract

Abstract: Glomus tumors are rare soft tissue neoplasms resembling the normal glomus body, which is a specialized form of arteriovenous anastomosis that regulates heat. The molecular genetics of sporadic glomus tumors has not been studied. We genotyped tumors from 28 patients (16 female patients and 12 male patients) ranging from 13 to 77 years and correlated the results with the tumor site (15 finger/1 hand/4 arm/7 leg/1 eyelid), Ki-67 index, and clinical follow-up. Tumor DNA from paraffin-embedded tissue was screened by multiplex polymerase chain reaction and mass spectroscopy, using a panel covering 370 mutations across 30 genes, including AKT1, BRAF, CTNNB1, EGFR, ERBB2, FGFR1/2/3, HRAS, KIT, KRAS, MEK1/2, NRAS, PDGFRA, and PIK3CA. A BRAF V600E mutation was identified in 3 cases, all of which occurred in proximal locations (upper shin, thigh, and upper arm). Two of the patients with BRAF-mutated tumors were quite young (21 and 13 years) and one of the BRAF-mutated tumors recurred in 3 years. A KRAS G12A mutation was found in tumor removed from the finger. Ki-67 index did not correlate with genotype. To our knowledge, this is the first report of oncogenic mutations in sporadic glomus tumors.

Author Information

Department of Pathology, Knight Cancer Institute, Oregon Health & Science University, Portland, OR.

C. L. Corless received an honorarium from Sequenom in 2009. No other conflicts of interest or sources of funding are declared.

Reprints: Andrea Chakrapani, MD, Department of Pathology, Knight Cancer Institute, Oregon Health & Science University, 3181 SW Sam Jackson Park Road, Portland, OR 97239 (e-mail: laueran@ohsu.edu).

© 2012 Lippincott Williams & Wilkins, Inc.