Skip Navigation LinksHome > February 2008 - Volume 30 - Issue 1 > The Role of β-1,4-Galactosyltransferase-I in the Skin Wound-...
American Journal of Dermatopathology:
doi: 10.1097/DAD.0b013e31815ae330
Original Article

The Role of β-1,4-Galactosyltransferase-I in the Skin Wound-healing Process

Shen, Aiguo PhD*‡; Qian, Ji MM†; Liu, Lei MM†; Liu, Haiou MM†; Chen, Jianping MM†; Niu, Shuqiong PhM*; Yan, Meijuan MD*; Chen, Xiaodong MD§; Shen, Congcong MM§; Gu, Jianxin PhD‡; Cheng, Chun MD†

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Cell-surface carbohydrate chains are known to contribute to cell migration, interaction, and proliferation. β-1,4-galactosyltransferase-I (β-1,4-GalT-I), which is one of the best-studied glycosyltransferases, plays a key role in the synthesis of type 2 chains in N-glycans and the core 2 branch in O-glycans. Recently, it has been reported that skin wound healing is significantly delayed in β-1,4-GalT-I−/− mice. However, the expression of β-1,4-GalT-I and its biological function in the skin wound-healing process remain to be elucidated. We used real-time polymerase chain reaction to demonstrate that the expression of β-1,4-GalT-I mRNA reached plateau values at 12 hours after skin was injured and remained elevated until 11 days after the injury. Furthermore, lectin blotting showed that β-1,4-galactosylated carbohydrate chains were also increased after skin injury. A double-staining method combining lectin-fluorescent staining with RCA-I and immunofluorescence was first used to determine the cellular localization of β-1,4-galactosylated carbohydrate chains. Morphological analysis showed that the chains were primarily expressed in neutrophils and partially expressed in macrophages, endothelial cells, and collagen. Our results suggest that β-1,4-GalT-I and β-1,4-galactosylated carbohydrate chains participate in leukocyte recruitment, angiogenesis, and collagen deposition in the skin wound-healing process.

© 2008 Lippincott Williams & Wilkins, Inc.


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