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Silver-Stained Nucleolar Organizer Regions in Hypertrophic and Keloid Scars

Ghazizadeh, Mohammad M.D., Ph.D.; Miyata, Nariaki M.D.; Sasaki, Yoshihiro B.S.; Arai, Katsuyuki M.D.; Aihara, Kaoru M.D.

American Journal of Dermatopathology:

Silver-stained nucleolar organizer regions (AgNORs) have been widely used as a marker of cellular activity and proliferation. In a retrospective study, we investigated the potential value of AgNORs in 12 hypertrophic and 24 keloid scar tissues. Ten normal skin tissues served as controls. A standard silver-staining method was used, and the mean AgNOR count of dermal fibroblastic cells in each tissue was determined. In normal skin, the mean AgNOR count of dermal fibroblasts was 1.79 ± 0.55, whereas fibroblastic cells in hypertrophic and keloid scars had mean AgNOR counts of 3.18 ± 0.56 and 5.10 ± 0.97, respectively. There was a statistically significant difference between the mean AgNOR counts of fibroblastic cells from normal skin, hypertrophic scar, and keloid scar [one-factor analysis of variance (ANOVA), p < 0.0001]. Our findings suggest that AgNOR count may be a useful marker for assessment of fibroblastic cell activity in hypertrophic and keloid scars, which may have potential value for histologic and biologic characterization of the two lesions.

Author Information

From the Central Institute for Electron Microscopic Researches, World Health Organization Collaborating Center for Research and Training in Diagnostic Electron Microscopy (M.G., Y.S., K.Ai.), Nippon Medical School, Tokyo; and Department of Traumatology and Critical Care Medicine (N.M., K.Ar.), National Defence Medical College, Saitama, Japan.

Address correspondence and reprint requests to Dr. Mohammad Ghazizadeh, Central Institute for Electron Microscopic Researches, Nippon Medical School, 1-1-5 Sendagi, Bunkyo-ku, Tokyo 113, Japan.

© Lippincott-Raven Publishers.