Poorly differentiated type synovial sarcoma (PDSS) is a variant of synovial sarcoma characterized by predominantly round or short-spindled cell morphology. Although accumulating evidence from clinicopathologic studies suggests a strong association between this variant of synovial sarcoma and poor prognosis, little has been reported on the molecular basis of PDSS. To gain insights into the mechanism(s) that underlie the emergence of PDSS, we analyzed the gene expression profiles of 34 synovial sarcoma clinical samples, including 5 cases of PDSS, using an oligonucleotide microarray. In an unsupervised analysis, the 34 samples fell into 3 groups that correlate closely with histologic subtypes: monophasic, biphasic, and poorly differentiated types. PDSS was characterized by down-regulation of genes associated with neuronal and skeletal development and cell adhesion. Moreover, upregulation of genes on a specific chromosomal locus, 8q21.11, was identified. This locus-specific transcriptional activation in PDSS was confirmed by reverse transcriptase-PCR analysis of 9 additional synovial sarcoma samples. Our results indicate that PDSS tumors constitute a distinct group based on expression profiles.
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*Genetics Division, National Cancer Center Research Institute
†Department of Orthopaedic Surgery, Keio University School of Medicine
§Orthopedic Division, National Cancer Center Hospital, Tokyo
‡Department of Surgical Pathology, Sapporo Medical University School of Medicine, Sapporo
∥Niigata University Graduate School of Medical and Dental Sciences, Niigata
¶Institute for Frontier Medical Sciences
♯Department of Orthopaedic Surgery, Graduate School of Medicine, Kyoto University, Kyoto, Japan
This work was supported by the program for promotion of Fundamental Studies in Health Sciences of the National Institute of Biomedical Innovation (NiBio) and by Grants-in-Aid from the Ministry of Education, Culture, Sports, Science and Technology, Japan.
Correspondence: Hitoshi Ichikawa, PhD, Genetics Division, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045, Japan (e-mail: firstname.lastname@example.org).
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There is no conflict of interest to declare.