HIV transmission and 24-month survival in a randomized trial of HAART to prevent MTCT during pregnancy and breastfeeding in Botswana
Shapiro, Roger L.a,b,c; Kitch, Douglasd; Ogwu, Anthonyc; Hughes, Michael D.d; Lockman, Shahinb,c,e; Powis, Kathleenb,c,f; Souda, Sajinic; Moffat, Clairec; Moyo, Sikhulilec; McIntosh, Kennethg; van Widenfelt, Erikc; Zwerski, Sherylh; Mazhani, Loetoi; Makhema, Josephb,c; Essex, Maxb,c
aDivision of Infectious Diseases, Beth Israel Deaconess Medical Center
bDepartment of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, Massachusetts, USA
cBotswana–Harvard AIDS Institute Partnership for HIV Research and Education, Gaborone, Botswana
dDepartment of Biostatistics, Harvard School of Public Health
eDepartment of Medicine, Division of Infectious Diseases, Brigham and Women's Hospital
fDepartments of Medicine and Pediatrics, Massachusetts General Hospital
gDivision of Infectious Diseases, Children's Hospital, Boston, Massachusetts
hNational Institutes of Health, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland, USA
iUniversity of Botswana School of Medicine, Gaborone, Botswana.
Correspondence to Roger Shapiro, MD, MPH, Associate Professor of Medicine, Beth Israel Deaconess Medical Center, Division of Infectious Diseases, 110 Francis St, Suite GB, Boston, MA 02215, USA. Tel: +1 617 771 0040; fax: +1 617 632 0766; e-mail: firstname.lastname@example.org
Received 29 January, 2013
Revised 11 March, 2013
Accepted 16 March, 2013
These data have been presented in part as: Shapiro RL, Kitch D, Hughes M, Ogwu, A, Lockman S, Souda S, Powis K, Moyo S, Makhema J, Essex M, and Mma Bana Study Team. Increased Maternal and Infant Mortality following Completion of HAART and Breastfeeding at 6 Months Postpartum in a Randomized PMTCT Trial: Botswana, the Mma Bana Study. 18th Conference on Retroviruses and Opportunistic Infections, Boston, Feb 27–Mar 2, 2011. Poster 747.
Objectives: HAART for prevention of mother-to-child HIV transmission (MTCT) may impact long-term survival of women and children.
Design: Randomized clinical trial.
Methods: HIV-infected pregnant women with CD4+ cell count at least 200 cells/μl were randomly assigned to abacavir, zidovudine, lamivudine (arm A) or lopinavir–ritonavir, zidovudine–lamivudine (arm B) from week 26 to 34 gestation through planned weaning by 6 months postpartum. Women with baseline CD4+ cell count less than 200 cells/μl received nevirapine–zidovudine–lamivudine indefinitely (Obs arm), as did randomized women later qualifying for treatment.
Results: Among 560 randomized and 170 observational women enrolled, there were 14 deaths (1.9%) – one antenatally (Obs), three from delivery to 6 months postpartum (1 arm A, 2 Obs), and 10 from 6 to 24 months postpartum (5 arm A, 3 arm B, 2 Obs). Time to death or CD4+ cell count below 200 cells/μl was shorter in arm A vs. B (P = 0.03). Of the 709 live-born children, 97% breastfed for a median of 5.8 months. Of 37 (5.2%) deaths by 24 months, nine were before breastfeeding initiated (3 arm A, 2 arm B, 4 Obs); six while breastfeeding (1 arm A, 2 arm B, 3 Obs); and 22 after weaning (9 arm A, 11 arm B, 2 Obs). Only eight children (1.1%) were HIV-infected at 24 months (6 arm A, 1 arm B, 1 Obs), all before 6 months.
Conclusion: Low MTCT was maintained through extended follow-up in all arms. Disease progression appeared slower after discontinuing protease inhibitor-based HAART, but a concerning number of maternal deaths occurred after stopping either regimen. Strategies to improve maternal and child survival in the postintervention period are required.
HAART started during pregnancy and continued through 6 months of breastfeeding can reduce mother-to-child HIV transmission (MTCT) as low as 1%, allowing safer breastfeeding where infant formula is unsafe or unavailable [1–6]. The WHO recommends maternal HAART or infant nevirapine prophylaxis as options to prevent breastfeeding MTCT , but limited data exist for long-term maternal and child outcomes with these strategies. Prevention of MTCT (PMTCT) beyond the intervention period, maternal survival after HAART cessation, long-term child survival, and avoiding potential toxicities remain important concerns [8–11].
We present final 24-month maternal and child outcomes from a randomized clinical trial of maternal HAART used for PMTCT in Botswana. Six-month virologic suppression and PMTCT findings were previously reported . This study includes final PMTCT efficacy results beyond the intervention period; CD4+ cell count and treatment re-initiation data over time; and prespecified morbidity and mortality comparisons through the end of follow-up at 24 months postpartum.
Trial design and study population
Between July 2006 and May 2008, HIV-infected, treatment-naive pregnant women choosing to breastfeed enrolled in the Mma Bana Study (meaning ‘mother of the baby’ in Setswana) in southern Botswana.
Women with CD4+ cell counts at least 200 cells/μl and no AIDS-defining illness were randomized to receive either abacavir (300 mg) (ABC)/zidovudine (300 mg) (ZDV)/lamivudine (150 mg) (3TC) co-formulated as Trizivir (GlaxoSmithKline, Greenford, United Kingdom) twice daily (arm A) or lopinavir (400 mg)/ritonavir (100 mg) (LPV/r) with ZDV (300 mg)/3TC (150 mg) co-formulated as Kaletra (Abbott Laboratories, Abbott Park, Illinois, USA)/Combivir (GlaxoSmithKline) twice daily (arm B). Randomized women initiated HAART between 26 and 34 weeks gestation and continued through weaning or 6 months postpartum (whichever first). Women meeting Botswana government treatment criteria [CD4+ cell counts <200 cells/μl (changed to <250 cells/μl after May 2008) or AIDS-defining illness] at any time continued or re-started HAART according to government guidelines. HAART for treatment was generally nevirapine (200 mg) (NVP)/ZDV (300 mg)/3TC (150 mg) twice daily (following a 2-week lead-in period of 200 mg once-daily NVP), but was individualized within available treatment guidelines.
Women with CD4+ cell counts below 200 cells/μl or with AIDS-defining illness received indefinitely the standard-of-care NVP-based treatment listed above from enrollment between 18 and 34 weeks gestation, and were followed observationally (Obs arm).
Infants received single-dose NVP (6 mg) at birth and ZDV (4 mg/kg twice daily) from birth to 4 weeks. HIV-infected children were offered HAART according to government treatment guidelines. Women were counseled to exclusively breastfeed and wean 3 days before the 6-month study visit. Infants were provided free formula from weaning and mothers were encouraged to introduce age-appropriate complementary foods beginning at 6 months.
The Health Research Development Committee from Botswana and the Harvard School of Public Health Human Subjects Committee approved the study protocol and amendments. An independent Data and Safety Monitoring Board reviewed safety and efficacy data approximately every 6 months. Participants signed written consent approved by the ethical review boards.
Study procedures and monitoring
Women were evaluated at least monthly from enrollment through delivery, and infants were enrolled and evaluated at birth. Women and children were evaluated at months 1–7, 9, 12, 15, 18, and 24. Telephone follow-up was conducted to determine 24-month vital status when unknown. After 6 months postpartum, maternal CD4+ cell count testing occurred at least 6-monthly (3-monthly for those off HAART with CD4+ cell counts <400 cells/μl). Infant HIV DNA PCR testing occurred at birth, 1, 6, and 12 months, and HIV ELISA at 18 months. Child hematology testing (hemoglobin, white blood cell count, absolute neutrophil count, and platelets) occurred at birth, 1, 3, 6, and 18 months. Additional laboratory monitoring was performed as previously described through 6 months .
Prespecified study objectives and definitions
Time to maternal and child death, child HIV infection or death, and time to first of death or CD4+ cell count below 200 cells/μl in women were prespecified study arm comparisons. Definitions for MTCT timing were as previously reported . Child weight for age z-scores were based on WHO norms  and small-for-gestational age assessment was determined by Botswana-specific norms .
Event rates were estimated using Poisson regression and differences between event rates were assessed using exact methods appropriate for small numbers of events. Factors associated with child mortality were assessed with a Poisson regression model fitted using generalized estimating equations. In this modeling, follow-up was divided into successive 1-month intervals starting from birth, and risk of death during an interval was evaluated by baseline and time-dependent factors; for the latter, the last available value at or before each interval was used. Univariate factors were first assessed, and those with P value less than 0.20 were included in multivariable models. Backwards variable selection was used to include factors with P value less than 0.05 in final models. For continuous variables, differences between means were assessed using the t-test. Time-to-event data were analyzed using the Kaplan–Meier estimator and the log-rank test. The proportion of patients with grade 3/4 events was compared using Fisher's exact test.
P values less than 0.05 were interpreted as statistically significant. Analyses were performed using SAS, Version 9.2 (SAS Institute, Cary, North Carolina, USA) and StatXact, Version 8.0 (Cytel Inc., Cambridge, Massachusetts, USA).
Role of the funding source
The study sponsor had no role in study design; in collection, analysis, or interpretation of data; or in writing or submitting this manuscript.
In total, 730 women were enrolled (285 arm A, 275 arm B, 170 Obs) (Fig. 1). Baseline characteristics were well balanced by randomization arm (Table 1). At 24 months, survival status was available for 714 (98%) women; 612 (84%) women had completed the protocol or died (including 19 of those with stillbirths), and 102 (14%) women were confirmed to be alive by telephone follow-up (Fig. 2a).
HAART treatment and CD4+ cell count changes
At 6 months postpartum, 13 women (5%) in arm A and 7 (3%) in arm B had met treatment criteria and continued HAART. Between 6 and 24 months, 31 (11%) in arm A and 33 (12%) in arm B re-started HAART (Fig. 2c). Thus 84 women (15%) in arms A and B received HAART after their PMTCT intervention completed: 81 met CD4+ cell count criteria to start, two had an AIDS-defining illness, and 1 started for PMTCT during another pregnancy. Among these 84 women, the median month for re-starting HAART was 12 months postpartum. Of those who re-started, three (4%) had a baseline CD4+ cell count at enrollment in pregnancy above 500 cells/μl, 12 (14%) from 351 to 500 cells/μl, 33 (39%) from 251 to 350 cells/μl, and 36 (43%) from 200 to 250 cells/μl. Although 85% in arms A and B remained off HAART after 6 months, mean CD4+ cell count increased from baseline to 24 months in all groups: +68 cells/μl (arm A), +100 cells/μl (arm B), and +282 cells/μl (Obs) (P = 0.11 for arm A vs. B). At 24 months or upon re-starting HAART (whichever first), mean CD4+ cell count change was significantly lower in arm A vs. arm B (+20 vs. +71 cells/μl; P = 0.005).
Maternal survival and adverse events
Fourteen mothers died (1.9%) (Table 2). One death occurred antenatally after HAART initiation (Obs); three between delivery and 6 months postpartum (1 arm A, 0 arm B, 2 Obs); and 10 between 6 and 24 months postpartum (5 arm A, 3 arm B, 2 Obs). Among randomized women, deaths occurred across baseline CD4+ cell count strata (four had baseline CD4+ cell count 200–350 cells/μl, two had baseline CD4+ cell count 351–500 cells/μl, and three had baseline CD4+ cell count >500 cells/μl). Most deaths among randomized women occurred after stopping HAART; eight deaths occurred from 6 to 24 months postpartum (1.0/100 person-years), whereas only one death occurred between HAART initiation and 6 months (0.3/100 person-years) [relative risk (RR) 3.8, 95% confidence interval (CI) 0.5, 167.2, P = 0.29]. Of the eight postintervention deaths, three had re-started HAART (0.9, 4.2, and 5.1 months prior to death) and five women had not re-started HAART as treatment at the time of death; the last CD4+ cell counts for these five women were 304, 399, 482, 518, and 886 cells/μl, drawn at a median of 3.8 months (range 0.4, 8.9) before death. Among all five observational women who died, the last CD4+ cell count before death was below 250 cells/μl. Causes of maternal death are detailed in a footnote to Table 2. In a planned analysis, time to death or CD4+ cell count below 200 cells/μl was shorter in arm A vs. arm B (P = 0.03); in arm A there were 21 CD4+ cell count events and six deaths, and in arm B there were 10 CD4+ cell count events and three deaths.
Grade 3/4 diagnoses, laboratory events, and hospitalizations were higher in the observational arm than in the randomized arms (Table 2). More neutropenias occurred in arm A compared with arm B (P = 0.014), but grade 3/4 events were otherwise similar by randomized treatment arm. For the entire study period, 82 (11.2%) women had a grade 3/4 diagnosis [29 (10.2%) arm A, 23 (8.4%) arm B, 30 (17.6%) Obs] with corresponding event rates per 100 person-years (95% CI) of 5.1 (3.4, 7.4), 4.2 (2.7, 6.3), and 8.4 (5.7, 12.0), respectively. Ninety-four women (13%) had a subsequent pregnancy.
Child breastfeeding and HIV infection
Of 709 live-born infants, survival status was available at 24 months for 691 (97%) children; 601 (85%) children had completed the protocol or died, and 89 (13%) were confirmed to be alive and one was confirmed to be dead at 24 months by telephone follow-up (Fig. 2b). Among 687 (97%) who breastfed, breastfeeding was stopped prior to 5 months in 173 (25%) and was reported beyond 7 months in only three (<1%) children (Fig. 2d). Only seven children breastfed after discontinuation of maternal HAART. In total, eight children (1.1%) were identified as HIV-infected through 18 months [6 (2.1%) arm A, one (0.3%) arm B, one (0.6%) Obs]. This number was unchanged from 6 months. Exposure to breast milk was reported in one child beyond the 18-month ELISA, but a subsequent postweaning ELISA was negative.
Child survival and adverse events
There were 37 (5.2%) child deaths in the study period. Of these, nine were before breastfeeding initiated (3 arm A, 2 arm B, 4 Obs), including seven children below 3 days of age. Six deaths occurred while breastfeeding (1 arm A, 2 arm B, 3 Obs), and 22 after weaning (9 arm A, 11 arm B, and 2 Obs). There was no significant difference in child survival between arms A and B (P = 0.71) or between randomized and observational arms (P = 0.69).
Child mortality was significantly increased after weaning. Thirteen deaths occurred within 3 months of weaning and nine occurred more than 3 months after weaning. No child had illness listed as a reason for weaning, and no deaths occurred within 5 days of last breastfeeding, making reverse causality (illness as a reason for weaning) unlikely. Among infants who initiated breastfeeding, the death rate during breastfeeding was 2.1/100 person-years, compared with 7.9/100 person-years within 3 months of weaning (RR 3.7, 95% CI 1.3, 12.0, P = 0.007). Beyond 3 months of weaning, the death rate declined to 1.0/100 person-years (compared with 7.9/100 person-years <3 months from weaning; RR 7.5, 95% CI 3.2, 18.4, P < 0.001). Child deaths are detailed in a footnote to Table 2.
Two of the eight HIV-infected children died. HIV infection or death occurred in 43 (6.1%) children; 18 (6.4%) arm A, 16 (5.9%) arm B, nine (5.8%) Obs. There was no significant difference in HIV-free survival between arms A and B (P = 0.74).
Grade 3/4 child diagnoses and hospitalizations did not differ significantly by maternal treatment arm (Table 2). For the entire study period, 111 children (15.7%) had a grade 3/4 diagnosis [47 (16.6%) arm A, 40 (14.8%) arm B, and 24 (15.4%) Obs] with a corresponding event rate per 100 person-years (95% CI) of 12.6 (9.7, 16.1), 9.3 (6.8, 12.5), and 9.9 (6.6, 14.3), respectively. Grade 3/4 child hematologic findings through 24 months did not differ by maternal study arm (P = 0.19). Beyond 6 months, child grade 3/4 anemia and neutropenia declined in all arms, occurring in 36 (5.5%) and 19 (2.9%) children, respectively. Through 24 months, 162 (22.8%) children were hospitalized, without a significant difference by maternal arm (P = 0.95).
Risk factors for child mortality
Risk factors for child mortality are presented in Table 3. Significant univariate risk factors included preterm delivery, being a twin, lower weight-for-age z-score, being within 3 months of weaning, and the child's age. Both weaning within 3 months (RR 4.07, 95% CI 1.62, 10.22) and the child's age by 3-month age group (P = 0.02) were significant risk factors for mortality when independently modeled, but co-linearity prevented their inclusion in the same model. Additional adjusted risk factors in the model including child age were preterm delivery (RR 3.4 for <37 vs. ≥37 weeks gestation, 95% CI 1.5, 7.8) and lower child weight for age z-score (RR 1.6 per 1 z-score lower, 95% CI 1.2, 2.2). Child mortality risk was greatest for children in the 6 to below 9 months age group.
Low MTCT was maintained through extended follow-up in all arms of this randomized clinical trial in Botswana, with no additional MTCT beyond the 6-month intervention period. Time to maternal death or CD4+ below 200 cells/μl was shorter in the triple nucleoside reverse transcriptase inhibitor (NRTI) arm than the protease inhibitor arm, and a concerning number of maternal deaths occurred after stopping HAART used for PMTCT. Child mortality was highest within 3 months of weaning.
Our MTCT findings are encouraging, and support the high efficacy of maternal HAART for preventing breastfeeding transmission when full viral suppression is achieved (93% of women in our study had HIV RNA <400 copies/ml throughout the breastfeeding period ). Breastfeeding transmission rates in our study are in accord with smaller studies [3,6] but were lower than other large clinical trials in Africa [2,4,8]. We believe that starting HAART by the early third trimester of pregnancy in most women, achieving high levels of HAART adherence and viral suppression through 6 months postpatum, and excellent compliance with the recommendation to wean by 6 months (with almost all children being weaned by 7 months) reduced MTCT risk in our study. In Malawi, where HAART from delivery to 28 weeks postpartum was evaluated and 48-week results reported, 4% overall MTCT occurred in the maternal HAART arm, with 30% of transmissions occurring after the intervention period . We can only explain the difference in postintervention MTCT rates by different levels of adherence to weaning recommendations in the two studies.
The randomized comparison between NRTI and protease inhibitor-based HAART was notable for several significant and nonsignificant trends favoring protease inhibitor-based PMTCT. A planned analysis of time to death or CD4+ cell count below 200 cells/μl favored protease inhibitor-based HAART, and appeared to be driven by a difference in the CD4+ cell count endpoint (11 more events in arm A) than death (three more endpoints in arm A). Although HIV RNA suppressed faster in arm A than in arm B , the overall CD4+ cell count increase was greater in arm B. Mortality, hospitalizations, and adverse events did not differ by randomization arm, but there was limited power to detect significant differences. The study was powered to detect virologic differences between randomized arms, and was not powered to detect a difference in MTCT. However, six arm A transmissions vs. one arm B transmission was noteworthy; further data are required to evaluate this difference.
Among randomized women, mortality increased after HAART was discontinued, with all but one death occurring in the postintervention period. This trend was not statistically significant, but the absolute mortality risk of 1.4% in the 18 months after stopping HAART is of concern. Current guidelines in Botswana , and newer WHO guidelines , support continuous treatment for women with CD4+ cell count below 350 cells/μl, providing greater security against rapid health declines before treatment initiation. These data are supported by several studies that highlight a survival benefit when HAART is started between CD4+ cell counts of 200–350 cells/μl rather than less than 200 cells/μl [15–17]. WHO also supports continuous treatment for all postpartum women following HAART initiation for PMTCT (Option B+) , and the fact that five deaths occurred among women with baseline CD4+ cell counts above 350 cells/μl may support this recommendation as well.
Although child mortality in our study was lower than others in Africa [2,4,8,10,19–21], we identified a clear period of risk during the first 3 months after weaning. The postweaning period for most children, from 6 to 9 months, had a higher mortality than the first 3 months of life, which is a striking finding that contrasts the normal pattern of reduced mortality risk as a child ages [22,23]. This same pattern has been described in other studies of HIV-exposed uninfected children after weaning [8,20,24–27]. Most children in our study were weaned before the 6-month visit, consistent with WHO recommendations at the time but earlier than current WHO guidelines developed by expert consensus. Few data exist to guide the decision about the optimal time to wean in the setting of ongoing MTCT risk. Relatively rapid weaning during the 3 days prior to the 6-month visit was also recommended (to avoid prolonged mixed feeding with formula or other foods), but it remains unknown how this strategy compares with a more gradual period of weaning.
Because of similarities in enrollment characteristics and location, cautious comparisons can be made with our previous PMTCT study (the Mashi Study) [24,28,29], where a non-HAART PMTCT intervention (ZDV with or without single-dose NVP) was used in the setting of maternal HAART availability for treatment (CD4+ cell count <200 cells/μl or AIDS) for most of the study. Although comparisons are underpowered for small differences, maternal deaths were similar in the two studies (1.9% in Mma Bana, 2.0% in Mashi breastfeeding arm). Child HIV infection or death through 24 months was significantly lower in the Mma Bana Study (6.1%) than in either the breastfeeding arm (15.0%) or formula feeding arm (13.5%) of the Mashi Study (both P < 0.001). Excluding HIV-infected children, there was also a trend for reduced child mortality in Mma Bana [5.0% in Mma Bana compared with 8.4% in Mashi formula feeding arm (P = 0.016) and 6.4% in Mashi breastfeeding arm (P = 0.32)]. Larger studies directly comparing maternal and infant PMTCT strategies may be required to determine whether maternal HAART has a positive impact on infant survival among HIV-exposed but uninfected infants.
The strength of our study was the randomized design and strict adherence to treatment and weaning recommendations by study participants. The most important limitation was lack of power to detect small differences between treatment groups, particularly a difference in MTCT. As with other studies conducted in Africa, determining exact causes of maternal and child mortality was a challenge because of limited diagnostic testing.
In summary, we report very low MTCT through 24 months of child age in all arms of this study. A significantly shorter time to maternal death or CD4+ cell count below 200 cells/μl was noted for women randomized to NRTI-based HAART, and despite relatively preserved 24-month CD4+ cell counts in the cohort as a whole, there did not appear to be a survival benefit for women beyond the intervention period. These findings support current WHO recommendations for a higher CD4+ cell count threshold to continue lifelong treatment , and to consider lifelong treatment for all women who start HAART for PMTCT . Child HIV-free survival in our trial surpassed any previous African PMTCT cohort of which we are aware, but it can still be improved. Further study is required to determine whether maternal HAART plays a role in promoting child survival, and whether the mortality risk after weaning can be reduced by biomedical interventions or by modification of weaning age and techniques.
Role of the authors: study design (R.S., M.H., S.L., K.M., S.Z., L.M., M.E.), study implementation (R.S., A.O., S.L., K.P., S.S., C.M., S.M., S.Z., J.M., M.E.), data management (E.W., K.P., D.K., A.O., C.M., S.S.), data analysis (R.S., D.K., M.H., S.L., K.P.), manuscript preparation (R.S., D.K., M.H., S.L., K.P., C.M., M.E.).
We are indebted to the women and children who participated in the Mma Bana Study, and thank: BHP and HSPH Staff: Jean Leidner, Esther Machakaire, Lillian Makori, Gloria Mayondi, Agnes Modise, Venice Modikwa, Ria Madison, Tlhongbotho Masoloko, Daisy Ramalekane, Molly Pretorius Holme, Heather Carey, Sara Mazzola, Carrie Kachoria, Raabya Rossenkahn, Vlad Novitsky, Chris Rowley, Jennifer Boyle, Michael Roy, Lendsey Melton, Chikezie Nwankwo, Scott Dryden Peterson, Onalenna Nthase, Norah Mawoko, Elias Woldegabriel, Kasonde Micheal, Chandan Harikrishnan, Jane Magetse, Joyce Lubinda, Tebogo Kakhu, Thena Tumediso, Modiegi Diseko, Mosetsanagape Galekhutle, Keamogetse Rebatenne, Mavis Moeng, Kebaibphe Ntalabgwe, Ditlamelo Mareme, Victoria Kgwadi, Kaone Kgati, Keitumetse Sakana, Best Mafoko, Lazarus Moremi, Jimmy Nkgau, Ilori Adewale, Banno Janet Moorad, Dipotso Arbi, Kesego Dudu Kooreng, Selebaleng Vinoliah Simon, Maggie Mosetsanagape Nkgau, Collen Rananna, Rejoice Molefe, Nametso Dimpho Lekwape, Tebogo Ncube, Eldah Kakanyo Tshotlego, Segomotso Mapote, Radinku Tshegofatso, Emmanuel Keikotlhae, William Keboutlwe, Hanqiwe Olebeng, Seleetso Ndicky Modibedi, Tshepo Silwane, Tshepiso Patricia Morupisis, Ntsholeng Kekgethile, Sydney Kgwefane, Julius Kgangetsile, Nnahurumnanya Iwe, Tseo Khudube, Malebogo Ntshimane, Maureen Gower, Nthabiseng Kgaodi, Kate Selathwe, Lorraine Phiri, Rosemary Musonda, Phillimon Segopodi , Dorcas Moses, Terence Mohammed, Dineo Mongwato, Bonolo Khumotaka , Phibeon Munyaradzi Mangwendeza, Chishamiso Mudenyanga, Matshediso Zachariah, Gertrude Ditshotlo, Alex Ntau, Poko Molwane. Botswana Ministry of Health: Khumo Seipone, Shenaaz El Halabi, Pilate Khulumani, Mary Kasule, Madisa Mine, Kgomotso Makhaola, Howard Moffat, Haruna Baba Jibril, and the PMTCT unit. Princess Marina Hospital, Gaborone: Staff of Maternity, Post natal & Children's ward. Scottish Livingstone Hospital, Molepolole: Staff of Maternity, Post natal & Children's Ward. Deborah Retief Memorial Hospital, Mochudi: Staff of Maternity, Postnatal & Children's ward. Athlone Hospital Lobatse: Staff of Maternity, Post natal & Children's ward. District Health Teams (Molepolole & Mochudi). City Council Clinics team (Lobatse & Gaborone). GSK: Edde Loeliger, Wendy Snowden. Abbott: Marisol Martinez-Tristiani. Baylor University: Gabriel Anabwani, Elizabeth Lowenthal. Oxford University: Philip Goulder, Philippa Mathews. NIH: Usha Sharma, Ed Handelsman (deceased), Lynne Mofenson. Brigham and Women's Hospital: Ruth Tuomola. DSMB Members: Susan Ellenberg (Chair), Jerrold Ellner, David Harrington, Elly Tebasoboke Katabira, Carl Jacobus Lombard, Mary Faith Marshall, Lucky Mokgatlhe, Andrew Nunn, Haroon Saloojee, Chewe Luo, Jerome Amir Singh and Rebecca DerSimonian (Executive Secretary).
Funding/Trial Registration: National Institute of Allergy and Infectious Diseases (U01-AI066454) / clinicaltrials.gov Identifier: NCT00270296.
Sponsorship: This material is based upon work supported by the National Institute of Allergy and Infectious Diseases, NIAID (U01-AI066454); by the Fogarty International Center grant D43 TW00004, which supported several of the trainees who were involved in this study; by GlaxoSmithKline (which provided Trizivir and Combivir); by Abbott Pharmaceuticals (which provided Kaletra); and by the Botswana Government (which provided Nevirapine, Combivir, additional medications, and some laboratory testing). Any opinions, findings, and conclusions or recommendations expressed in this publication are those of the authors and do not necessarily reflect the views of the sponsoring agencies/organizations.
Conflicts of interest
No authors have a commercial or other association that might pose a conflict of interest (e.g. pharmaceutical stock ownership, consultancy, advisory board membership, relevant patents, or research funding).
1. Shapiro RL, Hughes MD, Ogwu A, Kitch D, Lockman S, Moffat C, et al. Antiretroviral regimens in pregnancy and breast-feeding in Botswana
. N Engl J Med
2. The Kesho Bora Study GroupTriple antiretroviral compared with zidovudine and single-dose nevirapine prophylaxis during pregnancy and breastfeeding for prevention of mother-to-child transmission of HIV-1 (Kesho Bora study): a randomised controlled trial
. Lancet Infect Dis
3. Kilewo C, Karlsson K, Ngarina M, Massawe A, Lyamuya E, Swai A, et al. Prevention of mother-to-child transmission of HIV-1 through breastfeeding by treating mothers with triple antiretroviral therapy in Dar es Salaam, Tanzania: the Mitra Plus study
. J Acquir Immune Defic Syndr
4. Thomas TK, Masaba R, Borkowf CB, Ndivo R, Zeh C, Misore A, et al. Triple-antiretroviral prophylaxis to prevent mother-to-child HIV transmission through breastfeeding: the Kisumu Breastfeeding Study, Kenya: a clinical trial
. PLoS Med
5. Palombi L, Marazzi MC, Voetberg A, Magid NA. Treatment acceleration program and the experience of the DREAM program in prevention of mother-to-child transmission of HIV
2007; 21 (Suppl 4):S65–S71.
6. Arendt V, Ndimubanzi P, Vyankandondera J, Ndayisaba G, Muganda J, Courteille O, et al. AMATA study: effectiveness of antiretroviral therapy in breastfeeding mothers to prevent postnatal vertical transmission in Rwanda
. Fourth International AIDS Society Conference on HIV Treatment and Pathogenesis, Sydney, 22–25 July 2007. Abstract TuAX102.
8. Jamieson DJ, Chasela CS, Hudgens MG, King CC, Kourtis AP, Kayira D, et al. Maternal and infant antiretroviral regimens to prevent postnatal HIV-1 transmission: 48-week follow-up of the BAN randomised controlled trial
9. Heidari S, Mofenson LM, Hobbs CV, Cotton MF, Marlink R, Katabira E. Unresolved antiretroviral treatment management issues in HIV-infected children
. J Acquir Immune Defic Syndr
10. Omer SB. Twelve-month follow-up of Six Week Extended Dose Nevirapine randomized controlled trials: differential impact of extended-dose nevirapine on mother-to-child transmission and infant death by maternal CD4 cell count
11. Taha TE, Li Q, Hoover DR, Mipando L, Nkanaunena K, Thigpen MC, et al. Postexposure prophylaxis of breastfeeding HIV-exposed infants with antiretroviral drugs to age 14 weeks: updated efficacy results of the PEPI-Malawi trial
. J Acquir Immune Defic Syndr
13. Matthews LT, Ribaudo HJ, Parekh NK, Chen JY, Binda K, Ogwu A, et al. Birth weight for gestational age norms for a large cohort of infants born to HIV-negative women in Botswana compared with norms for U.S.-born black infants
. BMC Pediatr
14. Botswana Ministry of Health. 2012 Botswana National HIV & AiDS treatment guidelines.
Gaborone, Botswana: Ministry of Health.
15. Severe P, Juste MA, Ambroise A, Eliacin L, Marchand C, Apollon S, et al. Early versus standard antiretroviral therapy for HIV-infected adults in Haiti
. N Engl J Med
16. Hogg RS, Yip B, Chan KJ, Wood E, Craib KJ, O'Shaughnessy MV, et al. Rates of disease progression by baseline CD4 cell count and viral load after initiating triple-drug therapy
17. Sterne JA, May M, Costagliola D, de Wolf F, Phillips AN, Harris R, et al. Timing of initiation of antiretroviral therapy in AIDS-free HIV-1-infected patients: a collaborative analysis of 18 HIV cohort studies
19. Taha TE, Hoover DR, Chen S, Kumwenda NI, Mipando L, Nkanaunena K, et al. Effects of cessation of breastfeeding in HIV-1-exposed, uninfected children in Malawi
. Clin Infect Dis
20. Kuhn L, Aldrovandi GM, Sinkala M, Kankasa C, Semrau K, Mwiya M, et al. Effects of early, abrupt weaning for HIV-free survival of children in Zambia
. N Engl J Med
21. Rollins NC, Becquet R, Bland RM, Coutsoudis A, Coovadia HM, Newell ML. Infant feeding, HIV transmission and mortality at 18 months: the need for appropriate choices by mothers and prioritization within programmes
22. Jones G, Steketee RW, Black RE, Bhutta ZA, Morris SS. How many child deaths can we prevent this year?
23. Kuhn L, Coovadia HM. Protecting infants of HIV-positive mothers in Malawi
24. Thior I, Lockman S, Smeaton LM, Shapiro RL, Wester C, Heymann SJ, et al. Breastfeeding plus infant zidovudine prophylaxis for 6 months vs. formula feeding plus infant zidovudine for 1 month to reduce mother-to-child HIV transmission in Botswana: a randomized trial: the Mashi Study
25. Onyango-Makumbi C, Bagenda D, Mwatha A, Omer SB, Musoke P, Mmiro F, et al. Early weaning of HIV-exposed uninfected infants and risk of serious gastroenteritis: findings from two perinatal HIV prevention trials in Kampala, Uganda
. J Acquir Immune Defic Syndr
2009, Sep 25. [Epub ahead of print]
26. Homsy J, Moore D, Barasa A, Were W, Likicho C, Waiswa B, et al. Breastfeeding, mother-to-child HIV transmission, and mortality among infants born to HIV-Infected women on highly active antiretroviral therapy in rural Uganda
. J Acquir Immune Defic Syndr
27. Kafulafula G, Hoover DR, Taha TE, Thigpen M, Li Q, Fowler MG, et al. Frequency of gastroenteritis and gastroenteritis-associated mortality with early weaning in HIV-1-uninfected children born to HIV-infected women in Malawi
. J Acquir Immune Defic Syndr
28. Lockman S, Smeaton L, Shapiro R, Thior I, Wester C, Makhema J, et al. Risk factors for and timing of infant mortality among HIV-exposed children: a randomized clinical trial in Botswana
. 15th Conference on Retroviruses and Opportunistic Infections, Boston, 3–6 February 2008. Abstract 643.
29. Lockman S, Smeaton L, Shapiro R, Thior I, Wester C, Stevens L, et al., Mashi Study Team. Morbidity and mortality among infants born to HIV-infected mothers and randomized to breastfeeding versus formula-feeding in Botswana (Mashi Study)
. XVI International AIDS Conference, Toronto Canada, 13–18 August 2006. Abstract # TUPE0357.
Africa; antiretrovirals; Botswana; HIV; infant survival; maternal health; mother-to-child HIV transmission
© 2013 Lippincott Williams & Wilkins, Inc.
What does "Remember me" mean?
By checking this box, you'll stay logged in until you logout. You'll get easier access to your articles, collections,
media, and all your other content, even if you close your browser or shut down your
To protect your most sensitive data and activities (like changing your password),
we'll ask you to re-enter your password when you access these services.
What if I'm on a computer that I share with others?
If you're using a public computer or you share this computer with others, we recommend
that you uncheck the "Remember me" box.
Highlight selected keywords in the article text.
Data is temporarily unavailable. Please try again soon.