Epidemiology and Social
Missed opportunities to prevent mother-to-child-transmission: systematic review and meta-analysis
Wettstein, Celinaa; Mugglin, Catrinaa; Egger, Matthiasa,b; Blaser, Nelloa; Vizcaya, Luisa S.a; Estill, Jannea; Bender, Nicolea; Davies, Mary-Annb; Wandeler, Gillesa,c,*; Keiser, Oliviaa,*; for the IeDEA Southern Africa Collaboration
aDivision of International and Environmental Health, Institute of Social and Preventive Medicine (ISPM), University of Bern, Bern, Switzerland
bSchool of Public Health and Family Medicine, University of Cape Town, Cape Town, South Africa
cInfectious Diseases Clinic, Bern University Hospital, Bern, Switzerland.
*Gilles Wandeler and Olivia Keiser contributed equally to the writing of this article.
Correspondence to Olivia Keiser, Institute of Social and Preventive Medicine (ISPM), University of Bern, Finkenhubelweg 11, Bern CH-3012, Switzerland. Tel: +41 31 631 35 15; fax: +41 31 631 35 20; e-mail: firstname.lastname@example.org
Received 20 June, 2012
Revised 9 August, 2012
Accepted 23 August, 2012
Supplemental digital content is available for this article. Direct URL citations appear in the printed text and are provided in the HTML and PDF versions of this article on the journal's Website (http://www.AIDSonline.com).
Objectives: To determine magnitude and reasons of loss to program and poor antiretroviral prophylaxis coverage in prevention of mother-to-child transmission (PMTCT) programs in sub-Saharan Africa.
Design: Systematic review and meta-analysis.
Methods: We searched PubMed and Embase databases for PMTCT studies in sub-Saharan Africa published between January 2002 and March 2012. Outcomes were the percentage of pregnant women tested for HIV, initiating antiretroviral prophylaxis, having a CD4 cell count measured, and initiating antiretroviral combination therapy (cART) if eligible. In children outcomes were early infant diagnosis for HIV, and cART initiation. We combined data using random-effects meta-analysis and identified predictors of uptake of interventions.
Results: Forty-four studies from 15 countries including 75 172 HIV-infected pregnant women were analyzed. HIV-testing uptake at antenatal care services was 94% [95% confidence intervals (CIs) 92–95%] for opt-out and 58% (95% CI 40–75%) for opt-in testing. Coverage with any antiretroviral prophylaxis was 70% (95% CI 64–76%) and 62% (95% CI 50–73%) of pregnant women eligible for cART received treatment. Sixty-four percent (95% CI 48–81%) of HIV exposed infants had early diagnosis performed and 55% (95% CI 36–74%) were tested between 12 and 18 months. Uptake of PMTCT interventions was improved if cART was provided at the antenatal clinic and if the male partner was involved.
Conclusion: In sub-Saharan Africa, uptake of PMTCT interventions and early infant diagnosis is unsatisfactory. An integrated family-centered approach seems to improve retention.
In 2009, approximately 130 000 children were newly infected with HIV in sub-Saharan Africa, over 90% of them by vertical transmission [1,2]. If HIV-infected infants are left undiagnosed and untreated, about half of them die before the age of two . Mother-to-child transmission, which occurs in 20–45% of HIV-infected pregnant women without antiretroviral prophylaxis, can be lowered to 5% or less with adequate interventions . Early infant diagnosis (EID) and treatment after delivery dramatically reduce infant mortality . However, loss to follow-up of mothers and their infants from prevention of mother-to-child transmission (PMTCT) programs limits the impact of these interventions.
PMTCT interventions can be seen as a series of consecutive steps (Fig. 1). When pregnant women first present at an antenatal care unit, they are tested for HIV. If positive, they are counseled for PMTCT and provided with antiretroviral medication. They are also assessed for eligibility for lifelong combination antiretroviral therapy (cART) and if they are eligible, therapy is initiated. With the option B+ approach for PMTCT, this last step is unnecessary as all pregnant women start cART . EID of HIV by polymerase chain reaction (PCR) is performed on infants if their mothers return with them for postnatal follow-up, preferably at around 6 weeks after delivery. In infants who test negative at EID or if no EID is available, the final HIV status is determined after weaning. Finally, cART is initiated in HIV-infected infants.
At each step of the PMTCT cascade, pregnant women and their infants may be lost to follow-up and not benefit from important healthcare interventions. The magnitude and reasons for program attrition in sub-Saharan Africa remain poorly understood. We performed a systematic review and meta-analysis to evaluate the uptake of HIV testing and antiretroviral treatment in pregnant women and their children.
We searched Pubmed and Embase databases on 5 March 2012, limiting the search to publications in the English language and studies published since 2002 (i.e. when the scale-up of ART programs in sub-Saharan Africa began) . We used free text words as well as Medical Subjects Headings in Pubmed and Emtree-terms in Embase. We combined the following search terms and their variations: HIV, PMTCT, infection of newborn, pregnancy, prenatal care, postnatal care, antiretroviral agents, eligibility, referral process and loss to follow-up. We examined the references of all included studies. Further details on the search strategy are given in the web appendix, http://links.lww.com/QAD/A258.
We included all studies on pregnant women (with either unknown or positive HIV status) and their HIV-exposed infants who attended PMTCT programs in sub-Saharan Africa. We selected studies that reported the number of participants who were given access to at least one of the following interventions: initiation of antiretroviral prophylaxis for HIV-positive pregnant women; assessment and initiation of lifelong cART; or HIV testing in infants. We excluded qualitative studies, randomized controlled trials, modeling studies, studies where uptake of interventions was assessed by interview, and cost-effectiveness studies. Abstracts were screened according to a list of inclusion and exclusion criteria. Two reviewers independently assessed the eligibility of articles. Discrepancies were resolved by consensus.
Data extraction was performed in duplicate by eight reviewers using a standardized extraction sheet. The following data were extracted: characteristics of programs (setting, location, country) and participants (age, gestational age at first antenatal care visit), eligibility criteria for cART initiation and the number of participants completing the following steps: first, HIV-testing of pregnant women; second, initiation of antiretroviral prophylaxis for mothers; third, CD4 cell count testing; fourth, initiation of cART in eligible women; fifth, HIV diagnosis of the exposed infants around 6 weeks by PCR and between 12 and 18 months by PCR or antibody test; and sixth, cART initiation in infected infants (Fig. 1). Eligibility was defined according to the program threshold in use at the time of the study. We also extracted predictors of successful uptake of each step and recorded positive or negative associations with the outcomes studied. Discrepancies were resolved by consensus.
We assessed the percentage of participants who completed each of the steps, and estimated the underlying mean percentage by combining studies in random-effect meta-analysis. Calculations were done on the logit scale with results back-transformed to percentages. Results were heterogeneous with I2 values consistently above 90% and P values from tests of heterogeneity less than 0.001. We therefore, calculated approximate prediction intervals (PrI) based on the whole random effects distribution as well as traditional 95% confidence intervals (CI) around the mean of the distribution. PrI predict the likely underlying mean percentages in new studies and are the most sensible way to summarize the results of heterogeneous studies . The percentage of women tested for HIV in programs with opt-in and opt-out strategies were compared in a random-effects metaregression model. To calculate overall PMTCT coverage, all regimens (single dose nevirapine at birth, dual and triple antiretroviral prophylaxis, as well as cART prescribed for life) were considered. For the meta-analysis on testing between 12 and 18 months postpartum, only infants from programs without PCR-testing or with negative early PCR test result were included. Data were analyzed using STATA version 12 (StataCorp, College Station, Texas, USA).
Study and patient characteristics
We identified 146 potentially eligible full text articles out of 2370 identified studies based on titles and abstracts. Fifty-six publications met our inclusion criteria but a further 12 articles were excluded because they reported on the same populations and time periods as other articles. A total of 44 studies including 75 172 HIV-positive women were included. Details on the selection process are provided in the web appendix (Figure S1, http://links.lww.com/QAD/A258). The studies originated from 15 countries in sub-Saharan Africa (web appendix Figure S2, http://links.lww.com/QAD/A258). The number of patients in each publication ranged from 22 to 14 815 (Table 1) [7,8]. Eight studies reported on the gestational age at presentation to antenatal care [9–16]. The majority of women were in the late second or third trimester (web appendix Table S1, http://links.lww.com/QAD/A258). Many studies provided information on HIV testing (N = 27) or antiretroviral prophylaxis without cART-eligibility assessment (N = 23). Eligibility for cART was assessed in 14 programs, and 12 studies reported on cART-initiation in eligible women. Thirteen studies reported on uptake of EID, eight on HIV testing between 12 and 18 months postpartum and one study reported on treatment initiation in infected infants (Fig. 1).
Table 1-a Characteri...Image Tools
HIV testing of pregnant women
Table 1-b Characteri...Image Tools
In sites where provider initiated (opt-out) testing was performed, the combined estimate of women tested for HIV, based on nine studies, was 93.7%, with 95% CI of 92.4–95.0% and only slightly wider PrI of 88.7–98.6%. The combined estimate for opt-in testing, based on seven studies, was 57.6%, with a 95% CI of 40.0–75.1% and a PrI of 0–100% (Fig. 2). The results from the two testing strategies were clearly different (P < 0.001 from metaregression). Predictors of successful HIV testing were reported in six publications [10,17–21] and included cART provision at the antenatal clinic  and being accompanied by a male partner  (web appendix Table S2, http://links.lww.com/QAD/A258).
Antiretroviral prophylaxis for prevention of mother-to-child transmission
The combined estimate of the percentage of HIV-positive women receiving any type of prophylaxis, based on 34 studies, was 70.3%, with 95% CI and PrI of 64.3–76.3% and 33.7–100%, respectively, (Fig. 3, web appendix Figure S3, http://links.lww.com/QAD/A258). In 19 of these programs, the only PMTCT intervention consisted of single dose nevirapine (sdNVP) at birth. In three studies, women initiated lifelong cART if eligible and received sdNVP otherwise. Dual or triple ART prophylaxis was available in nine studies, with an estimate of mean coverage of 58.6% and 95% CI and PrI of 41.0–76.2% and 0–100%, respectively (Fig. 3, web appendix Figure S4, http://links.lww.com/QAD/A258). In six of these nine studies, some women received sdNVP even though dual or triple ART was available. The reason for this was that the women received sdNVP at their first visit but never returned for an assessment of ART eligibility [15,22]. In three of the 34 studies the antiretroviral regimen was not specified. Information on predictors of sdNVP uptake was available in six studies [10,13,17,19,20,23]. Uptake was better when the male partner was involved  and when women delivered at the healthcare facility  (web appendix Table S2, http://links.lww.com/QAD/A258).
Eligibility assessment and combination antiretroviral therapy initiation
Ten studies reported on uptake of assessments of cART eligibility based on CD4 cell counts and subsequent cART initiation. In these studies, a CD4 cell count was determined in 67.6% of HIV-infected pregnant women (95% CI 37.5–97.8%; PrI 0–100%). An estimated 22.3% of women who had a CD4 cell count were eligible for cART (95% CI 17.2–27.4%; PrI 3.0–41.6%) and an estimated 61.1% of these women initiated treatment (95% CI 47.7–74.5%; PrI 10.4–100%) (Fig. 3, web appendix Figure S5, http://links.lww.com/QAD/A258). Results were similar when including all studies with information on any one of these steps (web appendix Figure S6, http://links.lww.com/QAD/A258): the estimated percentage of HIV-positive women with a CD4 cell count, based on 14 studies, was 68.1% (95% CI 44.6–91.7%; PrI 0–100%). Among these 22.6% of women were eligible for cART (95% CI 17.8–27.5%; PrI 3.9–41.4%, based on 11 studies) and 61.5% of eligible women initiated cART (95% CI 49.8–73.2%; PrI 16.2–100, 11 studies). Six studies assessed whether women with CD4 cell measurements returned to collect the results: an estimated 72.6% (95% CI 62.5–82.7%; PrI 35.5–100%) returned to the clinic.
Four studies reported on predictors for having a CD4 cell count recorded [8,13,18,24]. A CD4 count was more likely to be recorded if the measurement was ordered on the same day as testing for HIV was done , if the result was available rapidly , if the test was done in urban areas , if the women was employed , or if the gestational age at the first visit was below 20 weeks . Five studies reported on predictors for cART initiation in eligible women [8,13,18,21,24,25]. Women were more likely to start cART if it was provided at the antenatal clinic [8,24], if they were older , had more children , or presented earlier in pregnancy  (web appendix Table S2, http://links.lww.com/QAD/A258).
Linkage between prevention of mother-to-child transmission services, infant HIV diagnosis and antiretroviral combination therapy initiation
The mean percentage of infants tested for HIV by PCR around 6 weeks based on 12 studies was 64.4% with 95% CI and PrI of 47.5–81.2% and 0–100%, respectively (Fig. 4, web appendix Table S3, http://links.lww.com/QAD/A258). Predictors for EID were reported in three studies [26–28]. These showed positive associations between uptake of testing and proximity to the clinic , large family size , early HIV diagnosis of the mother , or having received antiretroviral prophylaxis for PMTCT  (web appendix Table S2, http://links.lww.com/QAD/A258). The combined estimate of the percentage of infants tested between 12 and 18 months postpartum based on seven studies was 55.2% with 95% CI 36.4–74.1% and PrI 0.0–100.0% (Fig. 4, web appendix Table S3, http://links.lww.com/QAD/A258). Only one study followed the HIV-infected children until cART initiation: 29.5% of infected infants could be traced to an ART clinic and of these about half initiated cART .
Our meta-analysis included over 75 000 pregnant women from 15 countries in sub-Saharan Africa who had been enrolled in 44 studies of one or several steps of the PMTCT cascade. The results of the different studies were heterogeneous, and in some instances very heterogeneous, with maximally wide PrIs. Provider-initiated (opt-out) HIV testing of pregnant women resulted in much higher coverage than patient-initiated (opt-in) testing, with an estimated 94% of women being tested with the first approach compared to 58% with the second. The uptake of antiretroviral prophylaxis for mothers was unsatisfactory, and the percentage of cART-eligible patients who received the recommended treatment low. Overall, 70% of pregnant women received some form of antiretroviral prophylaxis, 64% of HIV-exposed infants accessed EID by PCR around 6 weeks postpartum and 55% were tested between 12 and 18 months.
The risk of mother-to-child HIV transmission can be dramatically reduced by preventive measures including antiretroviral prophylaxis for the mother and the child . According to a recent WHO update, the use of cART during pregnancy is preferable to mono or dual therapy . Our analysis showed that some women received sdNVP at their first visit and were then lost before dual or triple prophylaxis or cART could be initiated. In other cases it was unknown why the optimal regimen had not been provided even though it was available. Significantly, about one-third of patients did not receive any type of antiretroviral prophylaxis, and 40% of cART eligible women did not initiate treatment. This finding is in line with recent systematic reviews on retention in HIV care, which reported that between 38 and 88% of pregnant women , and 32 and 37% of eligible patients from the general HIV-infected population never started cART [31,32]. An important difference between pregnant women and the general HIV-infected population is that a substantially lower proportion of pregnant women were eligible for cART at first presentation (23 and 40% , respectively). This shows that with systematic HIV testing during pregnancy, HIV infection can be diagnosed earlier, providing opportunities to initiate cART before patients present with advanced disease.
In a previous review Hensen et al. compared HIV-testing uptake before and after implementation of the opt-out strategy and found a substantial increase in uptake, from 10 to 66%. In our analysis, which included studies with either opt-in or opt-out testing, we found that the mean uptake was 94% with the opt-out strategy. The consistency of results across programs was striking and in contrast to the pronounced heterogeneity of results with the opt-in strategy. In general, pregnant women may be reluctant to be tested for many reasons, including structural and sociocultural barriers [34,35]. However, the provision of universal opt-out HIV-testing in antenatal care clinics might remove some of the fears related to patient-initiated testing, as it ‘normalizes’ the testing process and integrates it into routine clinical care. As a consequence, women might be less afraid of being stigmatized and judged by their peers. The studies included in this analysis showed enhanced uptake of testing if patients were accompanied by their male partner . Finally, programmatic issues such as staff shortages or the unavailability of test kits might also limit HIV testing uptake .
In our meta-analysis, about two-thirds of the HIV-exposed infants returned for EID and even less for HIV testing between 12 and 18 months postpartum. We analyzed coverage of HIV infant testing in programs in which mothers were informed about the importance of testing their child. Preventing transmission from mothers who seroconvert while breastfeeding, or who are not attending an antenatal care clinic, is probably even more difficult than in women who attended an antenatal care clinic during pregnancy . Several strategies aimed at increasing the uptake of infant testing have been evaluated . For example, Rollins et al. showed that universal HIV-testing of infants at immunization clinics was acceptable and feasible in rural South Africa, with 90% of women agreeing to have their infant tested. WHO and most national guidelines recommend universal treatment of all HIV-infected children under 2 years of age. It is therefore important to develop effective strategies for testing children for HIV in infancy.
In order to increase the uptake of PMTCT interventions, our understanding of the individual and program-level factors that limit access to care must improve. Although few studies analyzed barriers to pre-ART care, integration of cART provision into routine antenatal services and reducing the number of visits seems promising. An example of this was the improved uptake of cART when the CD4 cell count was done on the same day as testing for HIV . Better uptake was also associated with the provision of cART at the same place as PMTCT services [8,21,24], or if the distance to the clinic was short [8,13]. In general, however, there is a lack of data on the effectiveness of integrating PMTCT programs with other health services. This is illustrated by a Cochrane review on this topic , which found only one study that matched the inclusion criteria. The involvement of the male partner may also increase the chance of successful interventions . In a study from Côte d’Ivoire, a family centered approach (i.e. provision of cART to partner and children and improved access to contraception) helped to achieve coverage of over 90% from CD4 measurement to EID.
Our review provides a unique overview on the uptake of diagnostic and interventional steps throughout the PMTCT cascade in sub-Saharan Africa. The studies included in this review represent a wide range of urban, semi-urban and rural healthcare settings from many regions of sub-Saharan Africa. There are, however, several limitations. As noted previously, there were important differences between studies, which led to heterogenous results and wide PrIs. Furthermore, the studies were conducted over 8 years, a period in which HIV testing, treatment and prevention strategies evolved substantially. For example, the studies evaluating opt-out HIV-testing were performed later than those with opt-in strategies, possibly leading to bias due to an increase in the level of HIV awareness and quality of care over time. Most of the studies did not trace patients lost to follow-up or assess predictors for losses to program. They could thus not contribute to a better understanding of the reasons for attrition. In the context of initiation of lifelong cART in eligible women, we did not consider different CD4 cell count thresholds for starting cART, and studies did not report whether eligibility was based on CD4 cell count or stage of disease. Finally, none of the studies evaluated the option B+ approach and only one study reported on cART initiation in HIV-infected infants.
In order to reach the UNAIDS goal of eliminating paediatric HIV infections by 2015 , the coverage of PMTCT and cART need to be improved. Even though provider-initiated HIV-testing shows promising results, large gaps in antiretroviral prophylaxis, cART initiation, and infant testing remain. In order to improve retention in care of HIV-infected mothers and prevent new HIV-infections in children, a better understanding of the major barriers is of paramount importance. Further research on the major reasons for the failure of PMTCT programs in sub-Saharan Africa is urgently needed.
We thank Kali Tal for editorial help and Marcel Zwahlen for statistical support.
C.W., C.M., O.K. analyzed the data. C.W., G.W., O.K. wrote the article. C.W., M.E., O.K. conceived and designed the systematic review. C.W., C.M., N.B., L.S., J.E., N.B., G.W., O.K. performed data extraction. M.E., M.D. revised the article.
The study was supported by the National Institute of Allergy and Infectious Diseases (NIAID), Grant 5U01-AI069924–05, a PROSPER fellowship grant to O.K. funded by the Swiss National Science Foundation (Grant 32333B_131629) and PhD fellowships to J.E. and N.B. from the Swiss School of Public Health and the Swiss National Science Foundation.
Conflicts of interest
The authors have no conflicts of interest to declare.
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