Skip Navigation LinksHome > September 24, 2010 - Volume 24 - Issue 15 > HIV-1 Gag evolution in recently infected human leukocyte ant...
AIDS:
doi: 10.1097/QAD.0b013e32833d8a38
Research Letters

HIV-1 Gag evolution in recently infected human leukocyte antigen-B*57 patients with low-level viremia

Durand, Christine Ma,*; O'Connell, Karen Aa,*; Apuzzo, Linda Gb; Langan, Susan Jb; Imteyaz, Hejabb; Ahonkhai, Aima Aa,†; Ceccato, Christina Ma; Williams, Thomas Mc; Margolick, Joseph Bb; Blankson, Joel Na

Free Access
Article Outline
Collapse Box

Author Information

aDepartment of Medicine, School of Medicine, USA

bDepartment of Molecular Microbiology and Immunolgy, Bloomberg School of Public Health, Johns Hopkins University, Baltimore Maryland, USA

cDepartment of Pathology, School of Medicine and Tricore Reference Laboratories, University of New Mexico, Albuquerque, New Mexico, USA.

*Christine M. Durand and Karen A. O'Connnell contributed equally to the writing of this article, USA.

Current address: Division of Infectious Disease, Massachusetts General Hospital, Boston, Massachusetts, USA.

Received 16 May, 2010

Revised 14 June, 2010

Accepted 22 June, 2010

Correspondence to Dr Joel N. Blankson, Broadway Research Building, Room #880, Johns Hopkins University School of Medicine, 722 N. Broadway, Baltimore, MD 21205, USA. Tel: +1 410 955 7757; fax: +1 443 287 6218; e-mail: jblanks@jhmi.edu

Collapse Box

Abstract

We studied viral evolution in five human leukocyte antigen (HLA)-B*57 patients recently infected with HIV-1. Escape mutations in HLA-B*57-restricted Gag epitopes were present at study entry in all patients, but were not associated with significant increases in viremia. Conversely, no new escape mutations in HLA-B*57-restricted epitopes or known compensatory mutations were detected in patients who experienced significant increases in viremia. Thus, the development of escape mutations alone does not determine virologic outcome in recently infected HLA-B*57 patients.

The human leukocyte antigen (HLA)-B*57 allele is over-represented in patients who naturally control HIV-1 infection [1]. Although CD8+ T cell responses to immunodominant HLA-B*57-restricted epitopes in HIV-1 epitopes are thought to play a significant role in the suppression of viral replication, escape mutations are present in HLA-B*57-restricted epitopes in virtually all HLA-B*57 individuals [1] and thus, it is not clear how control is maintained. Recent studies have shown that escape mutations develop shortly after infection [2] and two HLA-B*5701 patients [3] and an HLA-B*5801 patient [4] were shown to maintain exceptionally low viral loads despite the development of TW10 and IW9 Gag mutations during primary infection. However, all three patients were followed for less than 9 months and thus, the long-term effect of these mutations on the level of viremia is unknown.

We present a longitudinal sequence analysis from five recently infected treatment-naïve HLA-B*57 patients (clinical trial registry #NCT00106171). Early infection, defined as seroconversion in the past year, was confirmed by history and by the detuned assay [5]. All patients were infected with clade B isolates, and at the time of study entry had a median viral load of 217 copies/ml (range 171–742 copies/ml). The patients were followed for a median of 23 months (range 13–36 months). Sequence analysis of plasma virus was performed on at least two independent near full-length gag clones amplified at each time point. We specifically tracked escape mutations in well described HLA-B*57-restricted Gag epitopes as well as previously described compensatory mutations in the cyclophilin A-binding loop [6]. Reverse transcriptase and protease genes were also sequenced to rule out drug resistance mutations that could have affected viral fitness. Patient RH103 was noted to have the M184V mutation; no primary drug-resistance mutations were seen in the other patients.

As shown in Table 1, patients RH113, RH103, and X01 all had low viral loads despite having mutations in the TW10 and QW9 epitopes in plasma virus at the time of study entry (month 0). Evolution occurred in the IW9 epitope in patients RH113 and X01 and in the TW10 epitope in all three patients. In addition, isolates from patient RH103, who was also positive for the protective HLA-B*27 allele, developed the L268M escape mutation in the immunodominant HLA-B*27 restricted epitope KK10 at month 28. Despite the development of these new mutations in HLA-B*57-restricted Gag epitopes, all three patients maintained low viral loads for at least 13 months.

Table 1
Table 1
Image Tools

Patients RH014 and RH062 had significant increases in viremia during the period of study. As shown in Table 1, patient RH014 had a mixture of plasma viruses, including some isolates with a wild-type sequence at the earliest time point. Virus with T242N and G248N mutations in TW10 and the I147L mutation in the IW9 epitope was predominant at month 7. The A146P mutation that effects processing of IW9 was detected 3 months later. As with the three prior patients, no significant changes in their viral load were noted despite these mutations. However, at month 36, the patient was noted to have a more than 1 log increase in his viral load. No new mutations were found in HLA-B*57-restricted epitopes and no new compensatory mutations that restore the fitness of the T242N mutation [6] were found at this time point.

The first plasma clones amplified from patient RH062 had the A146P mutation as well as a P149 mutation in the IW9 epitope. He also had the G248 mutation in the TW10 epitope and the E312D mutation in the QW9 epitope. Despite this, his viral load at enrollment was just 171 copies/ml and it reached a nadir of 84 copies/ml 3 months later. However, his viral load increased to 1039 copies/ml at month 8, and to 3156 copies/ml at month 21. Sequence analysis at this time point revealed no new mutations in HLA-B*57-restricted epitopes and no new compensatory mutations.

Taken together, these data suggest that escape mutations in HLA-B*57-restricted epitopes alone do not explain virologic outcome. A similar finding was reported in a longitudinal study [7] of five HLA-B*5703 patients recently infected with clade C virus. However, the median viral load after virologic escape was 6784 copies/ml, which is more than a log higher than the median viral load of 405 copies/ml after escape in our cohort. Thus, our data emphasize the fact that remarkable virologic control can be achieved in early infection even in the presence of multiple escape mutations. More importantly, in the clade C cohort, development of mutations at position 163 of the KF11 epitope was associated with an increase in viral load [7]. This finding does not explain disease progression in HLA-B*57-positive patients infected with clade B virus as mutations at position A163 are rarely seen in clade B isolates [8] and the majority of chronic progressors maintain a wild-type sequence at the KF11 epitope [9,10]. Two patients in our cohort developed viral loads that were more than a log higher than the nadir values. Neither evolution in HLA-B*57-restricted epitopes, nor the development of known compensatory mutations could explain the increase in viremia in either case. The data presented here stand in contrast to our earlier case report of an HLA-B*5703 patient who maintained a viral load of less than 50 copies/ml for a year before ultimately progressing to a viral load of 13 000 copies/ml [11]. In that study, full viral genome sequence analysis before and after progression implicated mutations in the TW10 epitope as the cause of virologic breakthrough. This current study suggests that, in some cases, evolution in HLA-B*57-restricted epitopes alone does not explain virologic escape. Three patients maintained low viral loads for more than 11 months despite accruing multiple escape mutations in HLA-B*57-restricted epitopes, whereas two patients developed an increase in viremia in the absence of new mutations in HLA-B*57-restricted epitopes or known compensatory mutations. Factors such as reduced fitness of escape variants [6,12–17] and de novo CTL responses [18–20] to escape variants are probably important in the restriction of viral replication. A clearer understanding of the variables involved in the maintenance of virologic control and, in some cases, the loss of control in untreated HIV-infected HLA-B*57 patients, may lead to the development of effective HIV-1 vaccines.

Back to Top | Article Outline

Acknowledgements

This study was supported by the Center for AIDS Research grant number P30 AI42855 and National Institutes of Health grants R01AI056990-01A1 (J.B.M.), and R01 AI080328 (J.N.B.) as well as the General Clinical Research Center (grant UL1RR025005) at the Johns Hopkins School of Medicine. The nucleotide sequences determined for this study have been submitted to GenBank under accession numbers HM241761–HM241828.

Back to Top | Article Outline

References

1. O'Connell KA, Bailey JR, Blankson JN. Elucidating the elite: mechanisms of control in HIV-1 infection. Trends Pharmacol Sci 2009; 30:631–637.

2. Brumme ZL, Brumme CJ, Carlson J, Streeck H, John M, Eichbaum Q, et al. Marked epitope- and allele-specific differences in rates of mutation in human immunodeficiency type 1 (HIV-1) Gag, Pol, and Nef cytotoxic T-lymphocyte epitopes in acute/early HIV-1 infection. J Virol 2008; 82:9216–9227.

3. Goonetilleke N, Liu MK, Salazar-Gonzalez JF, Ferrari G, Giorgi E, Ganusov VV, et al. The first T cell response to transmitted/founder virus contributes to the control of acute viremia in HIV-1 infection. J Exp Med 2009; 206:1253–1272.

4. O'Connell KA, Xu J, Durbin AP, Apuzzo LG, Imteyaz H, Williams TM, et al. HIV-1 evolution following transmission to an HLA-B*5801-positive patient. J Infect Dis 2009; 200:1820–1824.

5. Janssen RS, Satten GA, Stramer SL, Rawal BD, O'Brien TR, Weiblen BJ, et al. New testing strategy to detect early HIV-1 infection for use in incidence estimates and for clinical and prevention purposes. JAMA 1998; 280:42–48.

6. Brockman MA, Schneidewind A, Lahaie M, Schmidt A, Miura T, Desouza I, et al. Escape and compensation from early HLA-B57-mediated cytotoxic T-lymphocyte pressure on human immunodeficiency virus type 1 Gag alter capsid interactions with cyclophilin A. J Virol 2007; 81:12608–12618.

7. Crawford H, Lumm W, Leslie A, Schaefer M, Boeras D, Prado JG, et al. Evolution of HLA-B*5703 HIV-1 escape mutations in HLA-B*5703-positive individuals and their transmission recipients. J Exp Med 2009; 206:909–921.

8. Yu XG, Lichterfeld M, Chetty S, Williams KL, Mui SK, Miura T, et al. Mutually exclusive T-cell receptor induction and differential susceptibility to human immunodeficiency virus type 1 mutational escape associated with a two-amino-acid difference between HLA class I subtypes. J Virol 2007; 81:1619–1631.

9. Migueles SA, Laborico AC, Imamichi H, Shupert WL, Royce C, McLaughlin M, et al. The differential ability of HLA B*5701+ long-term nonprogressors and progressors to restrict human immunodeficiency virus replication is not caused by loss of recognition of autologous viral gag sequences. J Virol 2003; 77:6889–6898.

10. Navis M, Schellens I, van Baarle D, Borghans J, van Swieten P, Miedema F, et al. Viral replication capacity as a correlate of HLA B57/B5801-associated nonprogressive HIV-1 infection. J Immunol 2007; 179:3133–3143.

11. Bailey JR, Zhang H, Wegweiser BW, Yang HC, Herrera L, Ahonkhai A, et al. Evolution of HIV-1 in an HLA-B*57-positive patient during virologic escape. J Infect Dis 2007; 196:50–55.

12. Leslie AJ, Pfafferott KJ, Chetty P, Draenert R, Addo MM, Feeney M, et al. HIV evolution: CTL escape mutation and reversion after transmission. Nat Med 2004; 10:282–289.

13. Martinez-Picado J, Prado JG, Fry EE, Pfafferott K, Leslie A, Chetty S, et al. Fitness cost of escape mutations in p24 Gag in association with control of human immunodeficiency virus type 1. J Virol 2006; 80:3617–3623.

14. Chopera DR, Woodman Z, Mlisana K, Mlotshwa M, Martin DP, Seoighe C, et al. Transmission of HIV-1 CTL escape variants provides HLA-mismatched recipients with a survival advantage. PLoS Pathog 2008; 4:e1000033.

15. Goepfert PA, Lumm W, Farmer P, Matthews P, Prendergast A, Carlson JM, et al. Transmission of HIV-1 Gag immune escape mutations is associated with reduced viral load in linked recipients. J Exp Med 2008; 205:1009–1017.

16. Boutwell CL, Rowley CF, Essex M. Reduced viral replication capacity of human immunodeficiency virus type 1 subtype C caused by cytotoxic-T-lymphocyte escape mutations in HLA-B57 epitopes of capsid protein. J Virol 2009; 83:2460–2468.

17. Crawford H, Lumm W, Leslie A, Schaefer M, Boeras D, Prado JG, et al. Evolution of HLA-B*5703 HIV-1 escape mutations in HLA-B*5703-positive individuals and their transmission recipients. J Exp Med 2009; 206:909–921.

18. Feeney ME, Tang Y, Pfafferott K, Roosevelt KA, Draenert R, Trocha A, et al. HIV-1 viral escape in infancy followed by emergence of a variant-specific CTL response. J Immunol 2005; 174:7524–7530.

19. Bailey JR, Williams TM, Siliciano RF, Blankson JN. Maintenance of viral suppression in HIV-1-infected HLA-B*57+ elite suppressors despite CTL escape mutations. J Exp Med 2006; 203:1357–1369.

20. Miura T, Brockman MA, Schneidewind A, Lobritz M, Pereyra F, Rathod A, et al. HLA-B57/B*5801 human immunodeficiency virus type 1 elite controllers select for rare gag variants associated with reduced viral replication capacity and strong cytotoxic T-lymphotye recognition. J Virol 2009; 83:2743–2755.

Cited By:

This article has been cited 3 time(s).

Journal of General Virology
Gag sequence variation in a human immunodeficiency virus type 1 transmission cluster influences viral replication fitness
Gijsbers, EF; van Nuenen, AC; Schuitemaker, H; Kootstra, NA
Journal of General Virology, 94(): 354-359.
10.1099/vir.0.048371-0
CrossRef
Journal of Virology
Pressure from TRIM5 alpha Contributes to Control of HIV-1 Replication by Individuals Expressing Protective HLA-B Alleles
Granier, C; Battivelli, E; Lecuroux, C; Venet, A; Lambotte, O; Schmitt-Boulanger, M; Delaugerre, C; Molina, JM; Chakrabarti, LA; Clavel, F; Hance, AJ
Journal of Virology, 87(): 10368-10380.
10.1128/JVI.01313-13
CrossRef
Cellular and Molecular Life Sciences
The implications of viral reservoirs on the elite control of HIV-1 infection
Buckheit, RW; Salgado, M; Martins, KO; Blankson, JN
Cellular and Molecular Life Sciences, 70(6): 1009-1019.
10.1007/s00018-012-1101-7
CrossRef
Back to Top | Article Outline

© 2010 Lippincott Williams & Wilkins, Inc.

Login