Non-Hodgkin lymphoma incidence in the Swiss HIV Cohort Study before and after highly active antiretroviral therapy

Polesel, Jerrya; Clifford, Gary Mb; Rickenbach, Martinc; Dal Maso, Luiginoa; Battegay, Manueld; Bouchardy, Christinee; Furrer, Hansjakobf; Hasse, Barbarag; Levi, Fabioh,i; Probst-Hensch, Nicole Mj; Schmid, Patrickk; Franceschi, Silviab; the Swiss HIV Cohort Study

doi: 10.1097/QAD.0b013e3282f2705d
Epidemiology and Social: CONCISE COMMUNICATION

Objective: To assess the long-term effect of HAART on non-Hodgkin lymphoma (NHL) incidence in people with HIV (PHIV).

Design: Follow-up of the Swiss HIV Cohort Study (SHCS).

Methods: Between 1984 and 2006, 12 959 PHIV contributed a total of 75 222 person-years (py), of which 36 787 were spent under HAART. Among these PHIV, 429 NHL cases were identified from the SHCS dataset and/or by record linkage with Swiss Cantonal Cancer Registries. Age- and gender-standardized incidence was calculated and Cox regression was used to estimate hazard ratios (HR).

Results: NHL incidence reached 13.6 per 1000 py in 1993–1995 and declined to 1.8 in 2002–2006. HAART use was associated with a decline in NHL incidence [HR = 0.26; 95% confidence interval (CI), 0.20–0.33], and this decline was greater for primary brain lymphomas than other NHL. Among non-HAART users, being a man having sex with men, being 35 years of age or older, or, most notably, having low CD4 cell counts at study enrolment (HR = 12.26 for < 50 versus ≥ 350 cells/μl; 95% CI, 8.31–18.07) were significant predictors of NHL onset. Among HAART users, only age was significantly associated with NHL risk. The HR for NHL declined steeply in the first months after HAART initiation (HR = 0.46; 95% CI, 0.27–0.77) and was 0.12 (95% CI, 0.05–0.25) 7 to10 years afterwards.

Conclusions: HAART greatly reduced the incidence of NHL in PHIV, and the influence of CD4 cell count on NHL risk. The beneficial effect remained strong up to 10 years after HAART initiation.

Author Information

From the aEpidemiology and Biostatics Unit, Aviano Cancer Center, Aviano, Italy

bInternational Agency for Research on Cancer, Lyon, France

cCoordination and Data Center, Swiss HIV Cohort Study, Lausanne

dDivision of Infectious Diseases, University Hospital Basel, Basel

eCancer Registry of the Canton of Geneva, Geneva

fKilink und Poliklinik für Infektiologie Universitätsspital, Bern

gDivision of Infectious Diseases and Hospital Epidemiology, University Hospital Zurich, Zurich

hCancer Registry of the Canton of Vaud, University of Lausanne, Lausanne

iCancer Registry of the Canton of Neuchâtel, Neuchâtel

jCancer Registry of the Canton of Zurich, Zurich

kKantonsspital FB Infektiologie/Spitalhygiene, St Gallen, Switzerland.

Received 20 June, 2007

Revised 7 September, 2007

Accepted 19 September, 2007

Correspondence to Dr Silvia Franceschi, International Agency for Research on Cancer, 150 cours Albert Thomas, 69372 Lyon cedex 08, France. E-mail:

Article Outline
Back to Top | Article Outline


Following the advent and widespread use of HAART, a decrease in HIV-associated morbidity and mortality was observed [1,2] including that due to non-Hodgkin lymphoma (NHL) [3–9]. Most studies of NHL so far, however, have used calendar period (e.g., before or after 1996 or 1997) as a proxy of HAART use [10], or have included relatively small numbers of NHL diagnosed after HAART initiation [2,8,11].

We therefore evaluated the long-term effect of HAART and its impact on known risk factors for NHL using data from the Swiss HIV Cohort Study (SHCS), a large prospective cohort with detailed follow-up information, including CD4 cell count and HAART use.

Back to Top | Article Outline

Materials and methods

The SHCS is an ongoing study that has been enrolling people infected with HIV (PHIV) over 16 years of age since 1988, with some retrospective enrolment going back to 1984, from seven large hospitals in Switzerland ( After written informed consent is obtained, detailed demographic, lifestyle, and clinical information is collected. Follow-up visits take place every 6 months and all AIDS-defining events, including NHL diagnosis and death, are recorded. The present study included PHIV enrolled up to 30 September 2005, and information recorded in the SHCS database up to 31 March 2006. PHIV were excluded from the present study if they: (1) did not have information on date of birth, gender or HIV-transmission category (n = 103); (2) were diagnosed with NHL at enrolment or earlier (n = 96); (3) had no follow-up visits (n = 682).

The majority of remaining NHL cases (n = 365) were identified from the SHCS database, but record linkage with eight Swiss Cantonal Cancer Registries [12] allowed for the identification of 64 additional NHL cases. Histological confirmation was mentioned in the majority of NHL cases, but histological subtype was often not available, or was reported using different classification systems [12]. Only primary brain lymphoma (PBL) could be systematically distinguished from other NHLs, and therefore, in the present study, results were reported for PBL and other NHL only. HAART was defined as a combination of at least three drugs, including a protease inhibitor or a non-nucleoside reverse transcriptase inhibitor. CD4 cell counts at enrolment in the SHCS, and at, or within 6 months prior to, HAART initiation and NHL diagnosis were retrieved. Antiretroviral treatment 1 month prior to NHL diagnosis was also retrieved.

For each subject, person-years (py) at risk were calculated between enrolment and NHL diagnosis, death, or last follow-up visit, whichever occurred first. Incidence rates per 1000 py were standardized for gender and age based on the enrolled population in the overall study period, using the direct method [13]. Ninety-five percent confidence intervals (CI) of incidence were computed according to the Poisson distribution [13]. The effect of various risk factors on NHL onset was assessed using hazard ratios (HR), estimated by means of the Cox proportional hazard model [14], and adjusted for SHCS centre, gender, age (in 5-year groups), and, when mentioned, CD4 count at enrolment (< 50, 50–99, 100–199, 200–349, ≥ 350 cells/μl, and unknown). Calendar period, HAART use, and time since HAART initiation were introduced as time-dependent variables. Person-years of HAART use can include periods where treatment was interrupted.

This study was approved by the local ethical committees of the collaborating clinics and of the International Agency for Research on Cancer.

Back to Top | Article Outline


The present study included 12 959 PHIV among whom 429 NHL cases were identified (incidence = 5.5 per 1000 py; 95% CI, 5.0–6.1). NHL incidence reached a peak of 13.6 per 1000 in 1993–1995 and declined thereafter down to 1.8 in 2002–2006. The number of intravenous drug users enrolled has decreased over the years, whereas the number of heterosexuals has increased, especially among women ( Eighteen percent of patients had AIDS at enrolment, and 23.5% developed it afterwards. Among people who developed AIDS during follow-up, NHL was the AIDS-defining illness in 201 (5.2%) (data not shown).

A large proportion (48.7%) of the person-years available derived from HAART users and Table 1 shows the incidence and HR of NHL by various characteristics separately in non-users and users of HAART. Incidence of NHL decreased from 8.8 per 1000 py in non-users to 1.9 per 1000 py in users, and the HR for users versus non-users was 0.26 (95% CI, 0.20–0.33). Among non-users of HAART, significantly increased HR for NHL were found among men (HR versus women = 1.94; 95% CI, 1.43–2.61), older individuals (HR ≥ 45 versus < 35 years = 2.71; 95% CI, 2.04–3.60), and men having sex with men (HR versus intravenous drug users = 1.81; 95% CI, 1.36–2.42). The associations of NHL risk with gender and HIV-transmission category were greatly attenuated and became non-statistically significant among HAART users, whereas the direct association with age did not change. The greatest difference between users and non-users was found in respect to the influence of CD4 cell count at study enrolment on NHL risk. Among non-users, NHL rates increased steeply with decreasing CD4 cell count from 4.3 per 1000 py in PHIV with ≥ 350 cells/μl to 39.0 per 1000 py in PHIV with < 50 cells/μl (HR = 12.26; 95% CI, 8.31–18.07). Among users, the association between CD4 cell count and NHL risk was not statistically significant (HR for CD4 < 50 versus ≥ 350 cells/μl = 1.21; 95% CI,: 0.61–2.43; Table 1). When we evaluated the relationship between NHL risk and CD4 cell count at HAART initiation, findings were similar to those for CD4 cell count at study enrolment (HR for < 50 versus ≥ 350 cells/μl = 1.66; 95% CI, 0.84–3.27; data not shown).

Figure 1 shows that among HAART users, compared to non-users, there was not only a decrease in the number of NHL cases, but a marked shift towards higher CD4 cell count at cancer diagnosis.

The decline in PBL after 1995 was stronger than for other NHL, i.e., PBL represented 31.6% of NHL before 1996, but only 13.3% in 1999–2006 (Table 2). Among non-users of HAART, the relationship between NHL risk and the degree of immunosuppression at study enrolment was stronger for PBL (HR for < 50 versus ≥ 200 cells/μl = 28.58; 95% CI, 15.36–53.18) than for other NHL (HR = 5.02; 95% CI, 3.13–8.06). Among users, neither the risk of PBL nor of other NHL was significantly associated with CD4 cell count, although the small number of PBL cases led to HR with very broad CI (Table 2).

Figure 2 shows HR for NHL in different periods after HAART initiation compared to non-users. The risk of NHL was already halved in the first 5 months of use and continued to decline thereafter (HR after 36–59 months = 0.10; 95% CI, 0.06–0.17). The reduction in NHL risk persisted unchanged up to 84–119 months after HAART initiation (HR = 0.12; 95% CI, 0.05–0.25).

Of the 96 HAART users who developed NHL, 75 (78.1%) were still receiving HAART 1 month before cancer diagnosis. Regimens included a protease inhibitor in 50, a non-nucleoside reverse transcriptase inhibitor in 12, and both types of drugs in 13 PHIV. Of the 21 individuals who developed NHL while no longer on HAART, five were taking at least one, and 16 were taking no antiretroviral drug 1 month before NHL diagnosis.

Back to Top | Article Outline


Our present study includes the largest number of NHL cases and person-years of HAART use ever evaluated in PHIV. Many studies have been conducted to determine whether the use of HAART has led to a change in NHL incidence among PHIV, similar to the striking decline seen in Kaposi's sarcoma. While some early studies did not report a decrease in NHL incidence up to the late 1990s [2,5,15–17], clear evidence of the beneficial effect of HAART on NHL incidence emerged soon after [3,4,9,11].

Record linkage studies of AIDS and cancer registries found that the relative risk of NHL among PHIV or people with AIDS, compared to the general population, approximately halved in Europe and the United States after the advent of HAART, although it remained greater than 20 [10,12,18]. HAART has also narrowed the gap in survival between NHL patients with and without HIV infection [19,20].

In the SHCS, NHL incidence reached 13.6 per 1000 py in 1993–1995 and then declined to less than 2 per 1000 py in the HAART era. HAART also diminished the variation in NHL risk by host characteristics. Among non-users, significantly increased NHL risks were found in men, individuals older than 45 years, men having sex with men and severely immunodepressed individuals. All these associations, except the one with age, were greatly reduced among users. The near complete disappearance of the association between CD4 cell count at enrolment or at initiation of antiretroviral treatment and NHL risk supports the strong efficacy of HAART regardless of the degree of immune impairment when follow-up or treatment begins. In fact, HAART diminished NHL incidence by approximately 60% among PHIV whose CD4 cell count at enrolment was ≥ 350 cells/μl, but by nearly 20-fold among those whose count was < 50 cells/μl. Thus, although it was already clear that HAART prevents NHL through improvement of immune status [20], this study shows that HAART avoids the majority of NHL, even among the most severely immunosuppressed individuals.

The availability of follow-up data until 2006 in the SHCS allowed us, for the first time, to estimate the persistence of the effect of HAART on the prevention of NHL up to 10 years after treatment initiation. While we confirmed that NHL risk is already halved in the first months of treatment, we found that the HR further declined during the first 3 years, and did not show signs of increasing again for at least 10 years.

PBL was nearly as frequent (17.1 per 1000 py) as other NHL (21.9 per 1000 py) in non-users whose CD4 cell count was < 50 cells/μl. By 1999, or among HAART users, PBL had become extremely rare (≤0.3 per 1000 py), as has been reported in studies from the United States [10,21].

Weaknesses of our present study include the lack of information on year of seroconversion, and the lack of NHL histology, which obliged us to combine all NHL other than PBL. Furthermore, we did not include information on adherence to HAART. Our computation of person-years of HAART use, therefore, includes periods where use was interrupted, and so treatment efficacy may be underestimated [22]. Indeed, we found that 22% of NHL in HAART users arose in persons who were no longer on HAART at cancer diagnosis. Our findings do reflect, however, the effectiveness of up to 10 years of HAART use to reduce NHL incidence in a cohort that is well representative of the general population of PHIV in Switzerland, as, since the beginning of the epidemic, 49% of PHIV and 68% of people with AIDS in Switzerland have been enrolled in the SHCS (

Back to Top | Article Outline


The authors thank A. Bordoni (Ticino), S. Ess (St Gallen), G. Jundt (Basel), A. Kofler and B. Ledergerber (Zurich) for help with lymphoma identification, and T. Perdrix-Thoma and L. Mei for technical assistance.

The members of the Swiss HIV Cohort Study are M. Battegay, E. Bernasconi, J. Böni, H.C. Bucher, P. Bürgisser, S. Cattacin, M. Cavassini, R. Dubs, M. Egger, L. Elzi, P. Erb, M. Fischer, M. Flepp, A. Fontana, P. Francioli (President of the SHCS, Centre Hospitalier Universitaire Vaudois, CH-1011- Lausanne), H. Furrer (Chairman of the Clinical and Laboratory Committee), M. Gorgievski, H. Günthard (Chairman of the Scientific Board), H. Hirsch, B. Hirschel, I. Hösli, C. Kahlert, L. Kaiser, U. Karrer, C. Kind, T. Klimkait, B. Ledergerber, G. Martinetti, B. Martinez, N. Müller, D. Nadal, M. Opravil, F. Paccaud, G. Pantaleo, M. Rickenbach (Head of Data Center), C. Rudin (Chairman of the Mother & Child Substudy), P. Schmid, D. Schultze, J. Schüpbach, R. Speck, P. Taffé, P. Tarr, A. Telenti, A. Trkola, P. Vernazza, R. Weber and S. Yerly

Sponsorship: This study was performed within the framework of the Swiss HIV Cohort Study, supported by the Swiss National Science Foundation (Grant 3347–069366), and was funded by a grant from OncoSuisse (ICP OCS 01355-03-2003) and the Istituto Superiore di Sanità, Rome, Italy (grant 20 G.3).

Conflicts of interest: The authors of this manuscript have no conflict of interest to declare.

Back to Top | Article Outline


1. Babiker A, Darbyshire J, Pezzotti P, Porter K, Rezza G, Walker SA, et al. Changes over calendar time in the risk of specific first AIDS-defining events following HIV seroconversion, adjusting for competing risks. Int J Epidemiol 2002; 31:951–958.
2. Ledergerber B, Egger M, Erard V, Weber R, Hirschel B, Furrer H, et al. AIDS-related opportunistic illnesses occurring after initiation of potent antiretroviral therapy: the Swiss HIV Cohort Study. JAMA 1999; 282:2220–2226.
3. International Collaboration on HIV and Cancer. Highly active antiretroviral therapy and incidence of cancer in human immunodeficiency virus-infected adults. J Natl Cancer Inst 2000; 92:1823–1830.
4. Kirk O, Pedersen C, Cozzi-Lepri A, Antunes F, Miller V, Gatell JM, et al. Non-Hodgkin lymphoma in HIV-infected patients in the era of highly active antiretroviral therapy. Blood 2001; 98:3406–3412.
5. Besson C, Goubar A, Gabarre J, Rozenbaum W, Pialoux G, Chatelet FP, et al. Changes in AIDS-related lymphoma since the era of highly active antiretroviral therapy. Blood 2001; 98:2339–2344.
6. Grulich AE, Li Y, McDonald AM, Correll PK, Law MG, Kaldor JM. Decreasing rates of Kaposi's sarcoma and non-Hodgkin's lymphoma in the era of potent combination antiretroviral therapy. AIDS 2001; 15:629–633.
7. Franceschi S, Dal Maso L, Pezzotti P, Polesel J, Braga C, Piselli P, et al. Incidence of AIDS-defining cancers after AIDS diagnosis among people with AIDS in Italy 1986–1998. J Acquir Immune Defic Syndr 2003; 34:84–90.
8. Diamond C, Taylor TH, Aboumrad T, Anton-Culver H. Changes in acquired immunodeficiency syndrome-related non-Hodgkin lymphoma in the era of highly active antiretroviral therapy: incidence, presentation, treatment, and survival. Cancer 2006; 106:128–135.
9. Carrieri MP, Pradier C, Piselli P, Piche M, Rosenthal E, Heudier P, et al. Reduced incidence of Kaposi's sarcoma and of systemic non-hodgkin's lymphoma in HIV-infected individuals treated with highly active antiretroviral therapy. Int J Cancer 2003; 103:142–144.
10. Engels EA, Pfeiffer RM, Goedert JJ, Virgo P, McNeel TS, Scoppa SM, et al. Trends in cancer risk among people with AIDS in the United States 1980–2002. AIDS 2006; 20:1645–1654.
11. CASCADE. Systemic non-Hodgkin lymphoma in individuals with known dates of HIV seroconversion: incidence and predictors. AIDS 2004; 18:673–681.
12. Clifford GM, Polesel J, Rickenbach M, Dal Maso L, Keiser O, Kofler A, et al. Cancer risk in the Swiss HIV Cohort Study: associations with immunodeficiency, smoking, and highly active antiretroviral therapy. J Natl Cancer Inst 2005; 97:425–432.
13. Breslow NE, Day NE. Statistical methods in cancer research, Vol. II: The design and analysis of cohort studies. Lyon: International Agency for Research on Cancer; 1987. IARC Scientific Publications No. 82.
14. Cox DR. Regression models and life-time tables. J Royal Stat Soc B 1972; 34:187–220.
15. Jacobson LP, Yamashita TE, Detels R, Margolick JB, Chmiel JS, Kingsley LA, et al. Impact of potent antiretroviral therapy on the incidence of Kaposi's sarcoma and non-Hodgkin's lymphomas among HIV-1-infected individuals. Multicenter AIDS Cohort Study. J Acquir Immune Defic Syndr 1999; 21(Suppl 1):S34–S41.
16. Matthews GV, Bower M, Mandalia S, Powles T, Nelson MR, Gazzard BG. Changes in acquired immunodeficiency syndrome-related lymphoma since the introduction of highly active antiretroviral therapy. Blood 2000; 96:2730–2734.
17. Ledergerber B, Telenti A, Egger M. Risk of HIV related Kaposi's sarcoma and non-Hodgkin's lymphoma with potent antiretroviral therapy: prospective cohort study. Swiss HIV Cohort Study. BMJ 1999; 319:23–24.
18. Dal Maso L, Franceschi S, Polesel J, Braga C, Piselli P, Crocetti E, et al. Risk of cancer in persons with AIDS in Italy, 1985–1998. Br J Cancer 2003; 89:94–100.
19. Biggar RJ, Engels EA, Ly S, Kahn A, Schymura MJ, Sackoff J, et al. Survival after cancer diagnosis in persons with AIDS. J Acquir Immune Defic Syndr 2005; 39:293–299.
20. Lim ST, Levine AM. Recent advances in acquired immunodeficiency syndrome (AIDS)-related lymphoma. CA Cancer J Clin 2005; 55:229–241.
21. Bower M, Powles T, Nelson M, Mandalia S, Gazzard B, Stebbing J. Highly active antiretroviral therapy and human immunodeficiency virus-associated primary cerebral lymphoma. J Natl Cancer Inst 2006; 98:1088–1091.
22. Kaufmann GR, Perrin L, Pantaleo G, Opravil M, Furrer H, Telenti A, et al. CD4 T-lymphocyte recovery in individuals with advanced HIV-1 infection receiving potent antiretroviral therapy for 4 years: the Swiss HIV Cohort Study. Arch Intern Med 2003; 163:2187–2195.

Cited By:

This article has been cited 6 time(s).

Continuing declines in some but not all HIV-associated cancers in Australia after widespread use of antiretroviral therapy
van Leeuwen, MT; Vajdic, CM; Middleton, MG; McDonald, AM; Law, M; Kaldor, JM; Grulich, AE
AIDS, 23(16): 2183-2190.
PDF (335) | CrossRef
JAIDS Journal of Acquired Immune Deficiency Syndromes
Immunologic and Virologic Predictors of AIDS-Related Non-Hodgkin Lymphoma in the Highly Active Antiretroviral Therapy Era
Engels, EA; Pfeiffer, RM; Landgren, O; Moore, RD
JAIDS Journal of Acquired Immune Deficiency Syndromes, 54(1): 78-84.
PDF (124) | CrossRef
Current Opinion in Oncology
Evolving epidemiology of malignancies in HIV
Bonnet, F; Chêne, G
Current Opinion in Oncology, 20(5): 534-540.
PDF (126) | CrossRef
Current Opinion in Infectious Diseases
Should HIV therapy be started at a CD4 cell count above 350 cells/μl in asymptomatic HIV-1-infected patients?
Sabin, CA; Phillips, AN
Current Opinion in Infectious Diseases, 22(2): 191-197.
PDF (119) | CrossRef
Current Opinion in Oncology
Smoke and mirrors: HIV-related lung cancer
Bazoes, A; Bower, M; Powles, T
Current Opinion in Oncology, 20(5): 529-533.
PDF (100) | CrossRef
JAIDS Journal of Acquired Immune Deficiency Syndromes
Survival After AIDS Diagnosis in Italy, 1999-2006: A Population-Based Study
Serraino, D; Zucchetto, A; Suligoi, B; Bruzzone, S; Camoni, L; Boros, S; Paoli, AD; Maso, LD; Franceschi, S; Rezza, G
JAIDS Journal of Acquired Immune Deficiency Syndromes, 52(1): 99-105.
PDF (345) | CrossRef
Back to Top | Article Outline

CD4; highly active antiretroviral therapy; HIV; non-Hodgkin lymphoma; Switzerland

© 2008 Lippincott Williams & Wilkins, Inc.