Skip Navigation LinksHome > March 8, 2002 - Volume 16 - Issue 4 > A randomized trial assessing the impact of phenotypic resist...
AIDS:
Clinical

A randomized trial assessing the impact of phenotypic resistance testing on antiretroviral therapy

Cohen, Calvin J.a; Hunt, Susanb; Sension, Michaelc; Farthing, Charlesd; Conant, Marcuse; Jacobson, Susanf; Nadler, Jeffreyg; Verbiest, Wernerh; Hertogs, Kurth; Ames, Michaeli; Rinehart, Alex R.j; Graham, Neil M.*; and the VIRA3001 Study Team

Free Access
Article Outline
Collapse Box

Author Information

From the aCommunity Research Initiative of New England, Boston, MA 02215, USA; bUniversity of Pittsburgh Medical Center, Pittsburgh, PA 15213, USA; cNorth Broward Hospital District, Fort Lauderdale, FL 33312, USA; dAIDS Healthcare Foundation, Los Angeles, CA 90027-6069, USA; eDermatology/HIV Consultations, San Francisco, CA 94117, USA; fEast Bay AIDS Center, Clinical Faculty, Department of Family and Community Medicine, Berkeley, CA 94705, USA; gUniversity of South Florida, Tampa, FL 33602, USA; hTibotec Virco NV, B-2800 Mechelen, Belgium; iGlaxoSmithKline, Research Triangle Park, NC 27709, USA; and jTibotec Virco USA, Durham, NC 27713, USA, formally at Glaxo Wellcome, Inc.

*For members of the VIRA3001 Study Team see Appendix.

The results of this study were presented in part in abstract 237 at the 7th Conference on Retroviruses and Opportunistic Infections, San Francisco, CA, USA, 30 January–2 February 2000.

Correspondence to: Neil Graham, MD, Vice President, Medical Department, Tibotec-Virco USA, 2505 Meridian Parkway, Suite 350, Durham, NC 27713, USA. Tel: +1 919 361 9010 (Ext 3245); fax: +1 919 361 9020; e-mail: neil.graham@tibotec-virco.com

Received: 23 March 2001;

revised: 1 October 2001; accepted: 3 October 2001.

Sponsorship: Provided by Glaxo Wellcome, Inc. (primary) and Virco NV.

Collapse Box

Abstract

Objective: To compare the effect of treatment decisions guided by phenotypic resistance testing (PRT) or standard of care (SOC) on short-term virological response.

Design: A prospective, randomized, controlled clinical trial conducted in 25 university and private practice centers in the United States.

Participants: A total of 272 subjects who failed to achieve or maintain virological suppression (HIV-1-RNA plasma level > 2000 copies/ml) with previous exposure to two or more nucleoside reverse transcriptase inhibitors and one protease inhibitor.

Interventions: Randomization was to antiretroviral therapy guided by PRT or SOC.

Main outcome measures: The percentage of subjects with HIV-1-RNA plasma levels less than 400 copies/ml at week 16 (primary); change from baseline in HIV-1-RNA plasma levels and number of ‘active’ (less than fourfold resistance) antiretroviral agents used (secondary).

Results: At week 16, using intent-to-treat (ITT) analysis, a greater proportion of subjects had HIV-1-RNA levels less than 400 copies/ml in the PRT than in the SOC arm (P = 0.036, ITT observed;P = 0.079, ITT missing equals failure). An ITT observed analysis showed that subjects in the PRT arm had a significantly greater median reduction in HIV-1-RNA levels from baseline than the SOC arm (P = 0.005 for 400 copies/ml;P = 0.049 for 50 copies/ml assay detection limit). Significantly more subjects in the PRT arm were treated with two or more ‘active’ antiretroviral agents than in the SOC arm (P = 0.003).

Conclusion: Antiretroviral treatment guided prospectively by PRT led to the increased use of ‘active’ antiretroviral agents and was associated with a significantly better virological response.

Back to Top | Article Outline

Introduction

The goals of treating HIV infection with antiretroviral drugs are to suppress HIV-1-RNA plasma levels (viral load) to undetectable levels for as long as possible, to restore or preserve immunological function, to improve quality of life, and to reduce HIV-related morbidity and mortality [1]. Current guidelines established by the Department of Health and Human Services call for the initiation and maintenance of antiretroviral therapy with one of the following types of regimens composed of three antiretroviral agents: two nucleoside reverse transcriptase inhibitors (NRTI) plus a protease inhibitor (PI); two NRTI plus a non-nucleoside reverse transcriptase inhibitor (NNRTI), or three NRTI [1]. Highly active antiretroviral therapy (HAART) regimens allow as many as 60–90% of antiretroviral-naive subjects in clinical trials and up to 44% of patients in inner-city clinic settings to achieve the maximal suppression of HIV-1-RNA levels [2,3]. Typically, a lower HIV-1-RNA nadir correlates with a longer time before drug failure occurs [4].

However, when antiretroviral drugs are given in combinations that only partly suppress HIV-1 replication, or when viral rebound occurs after initial successful suppression, viral mutations that confer drug resistance are selected and resistant viral strains can predominate within weeks, leading to poor clinical response and, ultimately, treatment failure [5–7]. In recent years, the prevention, characterization, and clinical management of resistance to HAART have received increasing attention. Testing for the resistance of HIV to antiretroviral drugs is now considered a rational adjunct to guide HAART [1,8,9]. Resistance is commonly measured in one of two ways. A sequencing (genotypic) analysis of the viral genome can identify point mutations that are known to be associated with resistance. As there are over 200 different mutations that are known to affect resistance and as these mutations can interact in complex ways, the interpretation of genotypic information can be highly complex and challenging [10].

Phenotypic resistance testing (PRT) utilizes an in vitro-based assay system, in which the 50% inhibitory concentration (IC50) to each antiretroviral agent is determined by culturing the recombinant viral strain in the presence of increasing concentrations of each drug. The IC50 for the recombinant virus is then compared with that of a genetically wild-type reference virus, to give the relative fold change in susceptibility of the subject's virus to each drug. PRT is a direct measure of HIV-1 drug susceptibility, and takes into account the net effect of resistance mutations and their interactions. Although these results can be used by most clinicians to help treatment decisions, expert advice is still generally recommended [8].

Testing a subject's virus for susceptibility to antiretroviral drugs and using this information to help make treatment decisions could reasonably be expected to help select active drugs, avoid drugs to which the subject's virus is resistant, and improve clinical outcome. Results from two published prospective trials of genotypic resistance testing VIRADAPT [11] and genotypic antiretroviral resistance testing (GART) [12] demonstrated an improvement in virological response when therapy decisions were guided by genotypic test results. Before VIRA3001, studies suggesting the potential clinical benefit of therapy guided by PRT were retrospective [13–15]. The VIRA3001 trial was an open-label, multicenter, randomized, controlled 16 week study designed to assess the impact on virological outcome of prospective PRT. The primary objective of the trial was to determine whether treatment guided by PRT results leads to greater viral load suppression than treatment guided by the standard of care (SOC), i.e. using treatment history, subject records and following published treatment recommendations, without PRT.

Back to Top | Article Outline

Methods

Study population

Eligible male and female subjects were 13 years of age or older, and had documented HIV-1 infection; HIV-1-RNA plasma levels of 2000 copies/ml or more; antiretroviral-experience; and were experiencing virological failure on antiretroviral treatment consisting of at least two NRTI and only one PI, taken for at least one month before screening. Subjects were excluded if they had a history of alcohol or drug use that was judged likely to interfere with therapy, had previous PRT, had participated in an antiretroviral drug trial within 30 days of selection or during the trial, had a life expectancy of less than 6 months, or had diseases that could interfere with assessments (e.g. lymphoma requiring ongoing chemotherapy, Kaposi's sarcoma requiring systemic therapy, active or life-threatening opportunistic infections, severe peripheral neuropathy, or cytomegalovirus retinitis). The protocol was amended to allow subjects with previous NNRTI therapy in May 1999. Four out of 272 randomly selected subjects had had previous genotypic testing. The study protocol was approved by Institutional Review Boards at all participating study sites, and all subjects provided written informed consent.

Back to Top | Article Outline
Study design

This open-label, randomized study was conducted over a 16 week treatment period at 25 study sites in the United States from January 1998 to September 1999, with a maximum of 30 randomly selected subjects per site. The primary objective of the study was to compare the virological outcome of antiretroviral regimens chosen with (PRT arm) or without (SOC arm) PRT. Investigators had access to published treatment guidelines and their subjects’ treatment histories (but not study-provided external ‘expert opinion') to assist treatment decision-making for subjects in both arms. At some sites treatment decisions were made by the investigator in collaboration with the subject's treating physician. The use of IL-2, granulocyte colony-stimulating factor, or granulocyte/macrophage colony-stimulating factor during the study was prohibited.

The endpoints for the study related to virological and immunological response. These included the proportion of subjects with HIV-1-RNA levels below 400 copies/ml at 16 weeks (primary endpoint). Secondary endpoints were absolute and average area under the curve minus baseline (AAUCMB) changes in HIV-1 RNA from baseline to week 16 and the degree and duration of immunological change (CD4 cell count), as assessed by absolute and AAUCMB changes from baseline to week 16. The proportion of subjects with HIV-1-RNA levels below 50 copies/ml at 16 weeks was also determined by re-analysing all week 16 samples with HIV-1-RNA levels below 5000 copies/ml using the Roche Amplicor Ultrasensitive assay (Roche Molecular Systems, Pleasanton, CA, USA). Baseline phenotypic resistance profiles in both groups were also evaluated.

Fig. 1 summarizes the study design. Study candidates were screened (informed consent, HIV-1-RNA plasma levels, and CD4 cell counts) 5 weeks before the initiation of new treatment regimens (week −5). A plasma sample was obtained at this time and submitted to Virco NV, Mechelen, Belgium, for PRT using the Antivirogram phenotypic assay. At week −4, eligible subjects were randomly assigned either to the PRT or SOC arms, demographic and medical history information was collected, and a physical examination and assessment of HIV clinical events were performed. Randomization was performed centrally, and occurred in blocks of size four (two subjects per treatment arm) independently at each site. Arm assignments were not blinded to either the investigator or subject. At week −2, before seeing the results of resistance testing, investigators completed a Choice of Therapy Survey, describing which treatment regimen they would prescribe at the time of change to a new regimen (irrespective of randomization). PRT results were then provided for subjects randomized to the PRT arm at week −1. At baseline, investigators were permitted to recommend a change in any treatment regimen component for subjects randomly assigned to either arm. Antiretroviral agents, which were tested for phenotypic susceptibility and were available for baseline regimen choices, were zidovudine, didanosine, zalcitabine, stavudine, lamivudine, abacavir, nevirapine, delavirdine, efavirenz, indinavir, ritonavir, saquinavir, nelfinavir, and amprenavir. All baseline treatment regimens were limited to either three or four antiretroviral drugs. A subtherapeutic, pharmacological enhancing dose of ritonavir (< 400 mg twice a day) was not considered as an antiretroviral drug. Plasma HIV-1-RNA levels (Amplicor HIV-1 Monitor, 400 copies/ml detection limit), CD4 cell count, and concomitant medication use were assessed at baseline, and at weeks 2, 4, 8, 12, and 16. Although the study was not powered for more sensitive virological cut-offs, an exploratory analysis was performed only on subjects with HIV-1-RNA plasma values of less than 5000 copies/ml (< 400 copies/ml detection limit) at week 16 using the Roche Amplicor Ultrasensitive assay (< 50 copies/ml limit of detection). Subjects were permitted to change baseline antiretroviral therapy during the 16 week period for reasons of intolerance or toxicity only. Baseline PRT results for subjects randomly assigned to the SOC arm were released to the investigator when the subject completed all 16 weeks of the study or failed to achieve virological suppression. All subjects were tested for phenotypic resistance upon completion of 16 weeks or after confirmed failed virological suppression. A lack of virological suppression was defined as the failure to achieve a 0.5 log10 or greater decrease below baseline at 8 weeks, a 0.5 log10 or greater increase from the lowest viral measurement achieved during the study, or the return of virus levels above baseline levels.

Fig. 1
Fig. 1
Image Tools
Back to Top | Article Outline
Phenotypic resistance testing

The Antivirogram assay was performed according to the standardized methodology [16,17]. Amplification of subject virus sequences included HIV-1 gag (p7/p1 and p1/p6 cleavage sites), protease, and reverse transcriptase (codons 1–400) sequences, covering all the known resistance mutations. A CD4 T cell line, MT-4, was transfected via electroporation with this part of the HIV-1 genome, together with an HIV-DNA construct from which this part of the genome was deleted. Upon intracellular recombination, progeny HIV-1 was produced within 5–10 days, and the newly formed chimeric viruses were analysed for phenotypic sensitivity to the 14 antiretroviral drugs in an automated, cellular-based assay. From the comparison of the subject's virus strain IC50 with the IC50 of the wild-type HIV-1 laboratory strain, a report showing the relative changes in susceptibilities (as fold changes in resistance) for each of the antiretroviral drugs was generated.

Back to Top | Article Outline
Sample size and statistical analysis

The percentage of subjects expected to be virological failures on the basis of the 400 HIV-1-RNA copies/ml of plasma detection limit during the first 16 weeks of the evaluation period was estimated to be 60% in the SOC arm and 40% in the PRT arm, on the basis of previous study findings [18–20]. To detect this difference with 80% power and a 5% two-sided significance level, 134 subjects were deemed necessary per treatment arm (after adjustment for a 20% dropout rate between random selection and 16 weeks of the study). A total study population of 268 subjects was thus planned. The recruitment of subjects was to continue until 134 subjects were randomly assigned to each treatment arm. The primary endpoint was virological response achieved at 16 weeks. In the analysis this was based on the percentage of subjects who achieved HIV-1-RNA values of less than 400 copies/ml of plasma in the two arms. Subjects who were exposed to antiretroviral drugs at baseline were included in the intent-to-treat (ITT) populations for efficacy analysis. In the ITT, missing equals failure (ITT, M = F) analysis, subjects were considered failures if they permanently discontinued the study for any reason, or had missing data, or failed to demonstrate virological suppression. In the ITT observed (ITTO) analysis, the change from baseline and AAUCMB were computed without imputing missing values; subjects who withdrew because of failure to demonstrate virological suppression were included in the denominator as ‘failures’ in the calculations of the percentage of subjects with HIV-1-RNA levels of less than 400 copies/ml of plasma.

The proportion of subjects with HIV-1-RNA levels below the limit of assay quantitation, as measured by quantitative HIV-1 RNA polymerase chain reaction, were compared across treatment arms using the Fisher's exact test. The median change from baseline to each study visit was reported for log10 HIV-1 RNA and CD4 cell count. The Wilcoxon rank sum test was used to compare the change from baseline and AAUCMB for log10 HIV-1 RNA and CD4 cell count between the study arms. Hodges–Lehmann estimators were used to compute 95% confidence intervals (CI) for the difference between study arms. A difference between the two treatment arms was considered statistically significant if the P value was less than 0.05. Additional post-hoc analyses examined the exploratory analysis carried out on subjects with HIV-1-RNA plasma values of less than 5000 copies/ml (< 400 copies/ml detection limit) at week 16 using the Roche Amplicor Ultrasensitive assay (< 50 copies/ml limit of detection), the effect of the PI initiated before study entry on virological outcome, and the effect of adding a NNRTI to the baseline regimen on virological outcome. The proportion of subjects receiving two or more ‘active’ antiretroviral agents, defined as agents to which the subject's virus exhibited less than a fourfold increase in resistance when compared with the standard wild-type reference virus, was also included in post-hoc analyses.

Back to Top | Article Outline

Results

Subject characteristics

In total, 142 and 130 subjects were randomly assigned to the PRT and SOC arms, respectively. Twenty-eight subjects (20%) in the PRT arm and 18 subjects (14%) in the SOC arm were removed from the ITT population as a result of withdrawal before or at baseline or the unplanned receipt of PRT result (one SOC subject) or missing PRT result (one PRT subject). Eighty-two subjects (43 in SOC and 39 in PRT arms; 30%) withdrew before study completion for the following reasons: lack of virological suppression (36 subjects, 13%); lost to follow-up (21 subjects, 8%); protocol violation (10 subjects, 4%); consent withdrawn (eight subjects, 3%); adverse event (five subjects, 2%); clinical progression (one subject); and other (one subject). Six subjects in the PRT arm and 11 subjects in the SOC arm changed baseline therapy as a result of intolerance or toxicity during the trial. The percentage of ITT subjects with previous exposure to PI was 53% for nelfinavir, 37% for indinavir, and 10% with other PI, whereas any previous exposure to NRTI was 96% for lamivudine, 84% for zidovudine, 61% for stavudine, 32% for didanosine, and 12% for zalcitabine. Previous NRTI use for over 2 years, 1–2 years, and less than 1 year was 41, 30, and 29%, respectively. Previous PI use for over 2 years, 1–2 years, and less than 1 year was 39, 33, and 28%, respectively. Four per cent of subjects (nine out of 226) had previously received NNRTI therapy. Four subjects in the PRT arm and seven subjects in the SOC arm used a subtherapeutic, pharmacological enhancing dose of ritonavir (< 400 mg twice a day) as part of their baseline regimen.

The demographic characteristics of the 114 subjects in the PRT and 112 subjects in the SOC arms who were exposed to antiretroviral drugs at baseline were similar between the two groups (Table 1). At baseline, the median plasma HIV-1-RNA level in the PRT arm was slightly higher (4.18 log10 copies/ml) than in the SOC arm (3.92 log10 copies/ml), and the median CD4 cell counts were nearly identical (348 versus 347 cells/mm3). Randomly selected subjects who were not exposed to antiretroviral therapy at baseline had median plasma HIV-1-RNA screening values of 3.80 log10 copies/ml (n = 27) and 4.35 log10 copies/ml (n = 17) for the PRT and SOC arms, respectively (P = 0.755).

Table 1
Table 1
Image Tools
Back to Top | Article Outline
Phenotypic susceptibility

At the screening visit, the proportion of subjects with virus that was susceptible (less than a fourfold increase in resistance) to PI was observed most frequently for amprenavir (92%) and saquinavir (84%), and a greater than 10-fold increase in resistance was observed most often (46% of subjects) for nelfinavir (Fig. 2a). In subjects who had virus with a greater than fourfold increase in resistance to indinavir or nelfinavir, susceptibility to amprenavir and saquinavir was consistently observed in the majority (Fig. 2b). Virus from most subjects demonstrated a greater than 10-fold increase in resistance to lamivudine (72%), whereas 79% of subjects remained susceptible to abacavir, 91% to didanosine, 94% to stavudine, and 70% to zidovudine (Fig. 2c). The numbers of subjects tested for phenotypic resistance to abacavir, amprenavir, and efavirenz were less than those observed for other antiretroviral agents because these antiretroviral agents were not added to the testing panel until Food and Drug Administration approval was obtained.

Fig. 2
Fig. 2
Image Tools
Back to Top | Article Outline
Influence of phenotypic resistance testing results on choice of antiretroviral therapy

Changes in antiretroviral therapy at baseline (the addition or subtraction of antiretroviral agents) between the predicted (week −2 Choice of Therapy Survey) and actual baseline regimens were significantly more frequent in the PRT arm when compared with the SOC arm with respect to overall treatment (76 versus 44%), NRTI (63 versus 34%), PI (55 versus 31%), and NNRTI (46 versus 24%) (P = 0.001 for all comparisons). Table 2 shows how phenotype results impacted treatment choices.

Table 2
Table 2
Image Tools

At baseline, significantly more subjects in the PRT arm when compared with the SOC arm were treated with two or more ‘active’ (less than fourfold increase in resistance) antiretroviral agents (92 versus 77%, P = 0.016). Similarly, a greater percentage of subjects in the PRT arm compared with the SOC arm were treated with two or more active NRTI (61 versus 40%, P = 0.005) and one or more active PI (60 versus 46%, P = 0.002). The median number of antiretroviral drugs administered at baseline was 3.4 in both treatment arms.

Back to Top | Article Outline
Virological outcome

In an ITT, M = F analysis, the percentage of subjects in the PRT arm with plasma HIV-1-RNA levels of 400 copies/ml or less at week 16 (46%) was greater than in the SOC arm (34%), but did not reach statistical significance [P = 0.079; 95% CI for the difference (PRT minus SOC) −1.0–24.4%]. In an ITTO analysis, significantly more subjects in the PRT arm had plasma HIV-1-RNA levels of 400 copies/ml or less (59 versus 43%, P = 0.036; 95% CI for the difference 1.9–30.9%) (Fig. 3b). The percentage of subjects with plasma HIV-1-RNA levels of 400 copies/ml or less was comparable in the two treatment arms from weeks 2 to 12 for both the ITT, M = F and ITTO analyses. In an exploratory analysis, the percentage of subjects with plasma HIV-1-RNA levels of 50 copies/ml or less at week 16 was comparable between the arms in the ITTO analysis (27 versus 28%).

Fig. 3
Fig. 3
Image Tools

An ITTO analysis revealed that the reduction in HIV-1-RNA plasma levels was consistently greater in the PRT arm than in the SOC arm from weeks 2 to 16 (Fig. 3a). At week 16, the decrease from baseline in plasma HIV-1-RNA levels was significantly greater in the PRT arm using assay detection limits of either 400 copies HIV-1-RNA/ml (P = 0.005, medians −1.23 versus −0.87 log10 copies/ml; 95% CI for the difference between study arms −0.72 to −0.13 ) or 50 copies HIV-1-RNA/ml (P = 0.049, medians −1.72 versus −1.21 log10 copies/ml; 95% CI for the difference between study arms −0.81 to −0.00). Similarly, an ITTO analysis of the log10 change in HIV-1-RNA levels using AAUCMB to week 16 also revealed a statistically significant difference with a less than 400 copies/ml assay detection limit (P = 0.012, medians −0.92 for PRT versus −0.72 for SOC; 95% CI for the difference between study arms −0.47 to −0.06).

Back to Top | Article Outline
Virological outcome by antiretroviral agent and antiretroviral classAn ITTO analysis of virological outcomes stratified by the PI initiated before study entry showed that the percentage of subjects who achieved plasma HIV-1-RNA levels of 400 copies/ml or less with previous exposure to indinavir was 55 and 28% (P = 0.040) in the PRT and SOC arms, respectively. The percentage of subjects who achieved plasma HIV-1-RNA levels of 400 copies/ml or less with previous exposure to nelfinavir was 63 and 50% (P = 0.298) in the PRT and SOC arms, respectively. Moreover, the median log10 change in HIV-1-RNA levels from baseline to week 16 was −1.20 and −0.34 (P = 0.009) in the PRT and SOC arms, respectively, for previous indinavir exposure, and −1.30 and −0.92 (P = 0.058) in the PRT and SOC arms, respectively, for previous nelfinavir exposure.

An additional analysis of ITTO data on the effect of adding a NNRTI to the baseline regimen demonstrated that when a NNRTI was not added, 49 and 23% (P = 0.015) of subjects achieved plasma HIV-1-RNA levels of 400 copies/ml or less in the PRT and SOC arms, respectively. In this subgroup of subjects not initiating NNRTI therapy, the median log10 change in HIV-1-RNA levels from baseline to week 16 was −1.07 and −0.26 (P = 0.003) in the PRT and SOC arms, respectively. When a NNRTI was added to the baseline regimen of NNRTI-naive subjects, 75 and 60% (P = 0.230) of subjects achieved plasma HIV-1-RNA levels of 400 copies/ml or less. The median log10 change in HIV-1-RNA levels from baseline to week 16 in this subgroup was −1.38 and −1.03 (P = 0.056) in the PRT and SOC arms, respectively.

Back to Top | Article Outline
Immunological outcome

At baseline, the median CD4 cell counts in the SOC and PRT treatment groups were similar (347 and 348 cells/μl, respectively). The median increase in CD4 cell counts from baseline to week 16 was 40 cells/μl for the SOC arm and 27 cells/μl for the PRT arm, and was not statistically different (P = 0.772).

Back to Top | Article Outline

Discussion

The results demonstrate that prospective PRT had a significant effect on improving virological response by several measures [absolute and AAUCMB log10 change in viral load from baseline and the proportion of subjects below detection (< 400 HIV-1-RNA copies/ml)]. In addition, physicians prescribed more ‘active’ drugs in the PRT arm, and the overall use of more active drugs was associated with a significantly better virological response both in terms of a greater reduction in HIV-1-RNA levels and the percentage of subjects attaining HIV-1-RNA plasma levels of less than 400 copies/ml.

The availability of prospective phenotypic test results had a considerable effect on the decision process. Overall, when compared with a hypothetical regimen chosen before baseline, a significantly larger number of changes were made to baseline regimens in the PRT arm compared with the SOC arm. This effect was more pronounced for certain antiretroviral agents (Table 2). For example, more abacavir and stavudine and less ritonavir and NNRTI were initiated as a result of the prospective PRT results.

Additional analyses based on antiretroviral class and individual antiretroviral agents were also conducted. In this mostly NNRTI-naive population, it was important to determine whether simply adding a NNRTI to the baseline regimen could outweigh the benefit of knowing prospectively the antiretroviral agents to which a subject might or might not respond. As expected, subjects who did not initiate a NNRTI had a dramatically improved virological response in the PRT arm compared with the SOC arm. However, in subjects initiating a NNRTI in the PRT arm, the difference in virological response compared with those in the SOC arm was not as marked.

Using a post-hoc analysis, virological response was also stratified by subjects who took either nelfinavir- or indinavir-containing regimens before study entry. A statistically significant improvement in virological response was observed in the PRT arm for those subjects on previous indinavir. However, there was less of an observed difference in virological response between arms for subjects who had previously received nelfinavir, although the trend again favored the PRT arm. This may suggest, at least among subjects with a lack of virological suppression on their first PI-containing regimen, that some initial regimens may result in less complicated resistance profiles, in which clinically guessing the next regimen may be successful.

The VIRA3001 trial was designed to emulate a clinical setting by not blinding the investigators or subjects to the randomization, by permitting subjects to change any part of their regimen at baseline, and by permitting investigators to interpret the PRT test results and ‘override’ them if desired. This is an important distinction between VIRA3001 and the prospective genotyping trials, in which treatment choices were recommended with the input of either a panel of experts in the GART or a rules-based algorithm in the VIRADAPT trials. The difference in HIV-1-RNA change from baseline between the intervention and control arms reached statistical significance in this trial as well as the prospective VIRADAPT and GART trials. Recent data from a large comparative trial (NARVAL) of phenotyping versus genotyping versus SOC showed no difference in the percentage of subjects (33% for phenotyping, 41% for genotyping, and 34% for SOC) with HIV-1-RNA plasma levels of less than 200 copies/ml at 12 weeks (P = 0.249) [21]. A major difference between this trial and the other prospective trials was the significantly greater antiretroviral experience, which may have limited treatment options. An underpowered study, as a result of incomplete enrollment, had similar limitations [22].

The question of whether the use of phenotypic or genotypic testing or both methodologies has greater clinical utility remains unresolved and is being addressed in ongoing clinical trials. PRT may offer some advantages, in that PRT results already take into account the net effect of any and all resistance mutations and their interactions. A limitation to phenotypic resistance testing has been the determination of cut-off values for resistance or susceptibility, which relate to clinical outcome. Historically, cut-off values were based on assay reproducibility and were the same for all antiretroviral agents, which for some antiretroviral agent (e.g. dideoxynucleoside analogues and NNRTI) represented an under- or over-reporting of resistance. Recent work has led to the determination of new drug-specific cut-off values [23,24]. Genotyping has a faster turnaround time, which may offset the interpretation limitations of this technology for some clinicians.

The study was not powered to detect differences in immunological response, and at 16 weeks immunological improvement was marginal and not significantly different between the arms.

It is noteworthy that overall only 54% of subjects (ITTO analysis) achieved plasma HIV-RNA levels of less than 400 copies/ml after 16 weeks of therapy. In the GART and VIRADAPT trials the percentage of subjects achieving HIV-1-RNA levels of between less than 500 copies/ml and 200 copies/ml of plasma was 46 and 32%, respectively. Both trials enrolled more highly antiretroviral-experienced subjects; 53% of subjects in GART and 46% of subjects in VIRADAPT were enrolled after the failure of their first PI-containing regimen. Although drug resistance accounts for a large proportion of all virological failures, several other causes of virological failure exist that would not be remedied by the use of prospective PRT in treatment decision-making. These include limited intrinsic antiviral potency, inadequate adherence, defective absorption of the antiretroviral drug (i.e. as a result of malabsorption or diarrhea), pharmacokinetic interactions, inadequate activation of the antiretroviral drug (i.e. intracellular phosphorylation), and viral replication at sanctuary sites.

An additional limitation of the trial is the short follow-up period. VIRA3001 was designed as a proof of concept trial in late 1997, at which time it was believed that a 16 week study would be sufficient to demonstrate any clinical value of prospective PRT. In addition, the issues of randomly assigning subjects to potentially suboptimal care, and the increased possibility of a learning effect over time also supported a shorter trial design.

A relatively high number of subjects (17%) discontinued before baseline therapy was initiated. This occurred in the earliest stages of the trial, and may have been caused by the concern involving the 4–5 week wait period on non-suppressive therapy before regimen component changes were permitted. Despite this concern, recently presented data showed that there was no significant difference in either HIV-1-RNA levels or genotypic mutational patterns between samples taken at screening and baseline visits [25]. This suggests that partly suppressive therapy can be continued during the period between taking a sample and receiving resistance test results without deleterious consequences. Although the percentage of subjects below detection was significantly greater in the PRT arm in the ITTO analysis (P = 0.036), statistical significance was not achieved in the ITT, M = F analysis (P = 0.079). The failure to reach statistical significance in one analysis and not the other was probably caused by the slightly different rates of discontinuation with respect to consent withdrawn and those lost to follow-up. Although exploratory analyses were carried out post-hoc using the viral load assay with a less than 50 copies/ml limit of detection, we did not expect to observe a difference because the study lacked the statistical power for this endpoint as a result of increased assay variability at these low levels. Nonetheless, with 226 subjects initiating 96 different baseline antiretroviral regimens among a group of subjects who had failed only one PI, the difference in virological response observed between the arms was remarkably clear-cut using the standard assay.

Back to Top | Article Outline

Conclusion

In HIV-infected subjects not responding to their first PI-containing HAART regimen, antiretroviral treatment choices guided by PRT were associated with a significantly better virological response than treatment choices guided by SOC. In view of this, PRT treatment guidance may be an important new clinical tool for determining the most appropriate HAART regimen for individuals not responding adequately to therapy.

Back to Top | Article Outline

Acknowledgements

The authors would like to thank the study participants and to acknowledge the VIRA3001 study investigators and study team personnel. They would also like to thank Gary E. Pakes, Pharm.D., for his assistance in the writing of this manuscript and Ashwin Hirani for his statistical programming assistance.

Back to Top | Article Outline

References

1. Department of Health and Human Services Panel on Clinical Practices for the Treatment of HIV Infection. Guidelines for the use of antiretroviral agents in HIV-infected adults and adolescents. January 2000. Available at http://www.hivatis.org.

2. Deeks SG, Hecht FM, Swanson M. et al. HIV RNA and CD4 cell count response to protease inhibitor therapy in an urban AIDS clinic: response to both initial and salvage therapy. AIDS 1999, 13: F35–F43.

3. Lucas GM, Chaisson RE, Moore RD. Highly active antiretroviral therapy in a large urban clinic: risk factors for virologic failure and adverse drug reactions. Ann Intern Med 1999, 131: 81–87.

4. Kempf DJ, Rode RA, Xu Y. et al. The duration of viral suppression during protease inhibitor therapy for HIV-1 infection is predicted by plasma HIV-1 RNA at the nadir. AIDS 1998, 12: F9–F14.

5. Condra JH, Schleif WA, Blahy OM. et al. In vivo emergence of HIV-1 variants resistant to multiple protease inhibitors. Nature 1995, 374: 569–571.

6. Havlir DV, Gamst A, Eastman S, Richman DD. Nevirapine-resistant human immunodeficiency virus: kinetics of replication and estimated prevalence in untreated subjects. J Virol 1996, 70: 7894–7899.

7. Wei X, Ghosh SK, Taylor ME. et al. Viral dynamics in HIV-1 infection. Nature 1995, 373: 117–122.

8. Hirsch MS, Brun-Vezinet F, D'Aquila RT. et al. Antiretroviral drug resistance testing in adult HIV-1 infection. JAMA 2000, 283: 2417–2426.

9. Rodriguez-Rosado R, Biones C, Soriano V. Introduction of HIV drug-resistance testing in clinical practice. AIDS 1999, 13: 1007–1014.

10. Schinazi RF, Larder BA, Mellors MW. Mutations in retroviral genes associated with drug resistance: 2000–2001 update. Int Antiviral News 2000, 8: 65–91.

11. Durant J, Clevenburgh P, Halfon P. et al. Drug-resistance genotyping in HIV-1 therapy: the VIRADAPT randomised controlled trial. Lancet 1999, 353: 2195–2199.

12. Baxter JD, Mayers DL, Wentworth DN. et al. A randomized study of antiretroviral management based on plasma genotypic antiretroviral resistance testing in patients failing therapy. AIDS 2000, 14: F83–F93.

13. Harrigan PR, Hertogs K, Verbiest W. et al. Baseline HIV drug resistance profile predicts response to ritonavir–saquinavir protease inhibitor therapy in a community setting. AIDS 1999, 13: 1863–1871.

14. Deeks SG, Grant RM, Beatty GW, Horton C, Detmer J, Eastman S. Activity of a ritonavir plus saquinavir-containing regimen in patients with virologic evidence of indinavir or ritonavir failure. AIDS 1998, 12: F97–F102.

15. Degruttola V, Dix L, D'Aquila RD. et al. The relation between baseline HIV drug resistance and response to antiretroviral therapy: re-analysis of retrospective and prospective studies using a standardized data analysis plan. Antiviral Ther 2000, 5: 41–48.

16. Kellam P, Larder BA. Recombinant virus assay: a rapid, phenotypic assay for assessment of drug susceptibility of human immunodeficiency virus type 1 isolates. Antimicrob Agents Chemother 1994, 38: 23–30.

17. Hertogs K, Debethune MP, Miller V. et al. A rapid method for simultaneous detection of phenotypic resistance to inhibitors of protease and reverse transcriptase in recombinant human immunodeficiency virus type 1 isolates from patients treated with antiretroviral drugs. Antimicrob Agents Chemother 1998, 42: 269–276.

18. Tebas P, Patick AK, Kane EM, et al. Virologic responses to a ritonavir–saquinavir-containing regimen in patients who had previously failed nelfinavir. AIDS 1999, 13:F23–F28.

19. Puig T, Bonjoch A, Ruiz L, et al. Usefulness of ritonavir and saquinavir combination therapy in HIV advanced patients failing on indinavir. In:37th Interscience Conference on Antimicrobial Agents and Chemotherapy. Toronto, October 1997 [Abstract I-201].

20. Hammer S, Squires K, Degruttola V, et al. Randomized trial of abacavir (ABC) and nelfinavir (NFV) in combination with efavirenz (EFV) and adefovir dipivoxil (ADV) as salvage therapy in patients with virologic failure receiving indinavir. In:6th Conference on Retroviruses and Opportunistic Infections. Chicago, February 1999 [Abstract 490].

21. Meynard JL, Vray M, Morand-Joubert L, et al. Impact of treatment guided by phenotypic or genotypic resistance tests on the response to antiretroviral therapy: a randomized trial (NARVAL, ANRS 088). In:4th International Workshop on HIV Drug Resistance and Treatment Strategies. Sitges, June 2000 [Abstract 85].

22. Melnick D, Rosenthal J, Cameron M, et al. Impact of phenotypic antiretroviral drug resistance testing on the response to salvage antiretroviral therapy (ART) in heavily experienced patients. In:7th Conference on Retroviruses and Opportunistic Infections. San Francisco, January 2000 [Abstract 786].

23. Larder BA, Harrigan RH. Biologically relevant cut-off values for phenotypic HIV-1 drug susceptibility testing based on the variation in drug susceptibility in untreated individuals. In:3rd European Symposium on the Clinical Implications of HIV Drug Resistance. Frankfurt, February 2001 [Abstract 36].

24. Lanier ER, Hellman N, Scott J, et al. Determination of a clinically relevant phenotypic resistance ‘cutoff’ for abacavir using the Phenosense assay. In:8th Conference on Retroviruses and Opportunistic Infections. Chicago, February 2001 [Abstract 254].

25. Hertogs K, Verbiest W, De Vroey V, et al. Continuing drug pressure did not alter HIV drug resistance profiles between screening and baseline visits in the prospective trial VIRA3001. In: 40th Interscience Conference on Antimicrobial Agents and Chemotherapy. Toronto, September 2000 [Abstract 1262].

Back to Top | Article Outline
Appendix

Members of the VIRA3001 Study Team: Roberto Arduino, MD (Houston, TX, USA), Carol Brosgart, MD, (Berkeley, CA, USA), Stephen Brown, MD (West Hollywood, CA, USA); Calvin Cohen, MD (Brookline, MA, USA), Ann Collier, MD (Seattle, WA, USA); Marcus Conant, MD (San Francisco, CA, USA), Steven Davis, MD (Dallas, TX, USA); Charles Farthing, MD (Los Angeles, CA, USA), Jeffrey Galpin, MD (Tarzana, CA, USA); Jeffrey Goodgame, MD (Altamonte Springs, FL, USA); Howard Grossman, MD (New York, NY, USA); W. Keith Henry, MD (St Paul, MN, USA); Susan Hunt, MD (Pittsburgh, PA, USA); Susan Jacobson, MD (Berkeley, CA, USA); Harold Kessler, MD (Chicago, IL, USA); Martin Markowitz, MD (New York, NY, USA); Douglas Mayers, MD (Detroit, MI, USA); Jeffrey Nadler, MD (Tampa, FL, USA), Michael Saag, MD (Birmingham, AL, USA); Shannon Schrader, MD (Houston, TX, USA); Michael Sension, MD (Fort Lauderdale, FL, USA), Allan Stein, DO (Coral Gables, FL, USA); Richard Stryker, MD (Beverly Hills, CA, USA); Melanie Thompson, MD (Atlanta, GA, USA); Gabriel Torres, MD (New York, NY, USA); Deborah Copeland (Glaxo Wellcome Inc., Research Triangle Park, NC). Cited Here...

Cited By:

This article has been cited 115 time(s).

Jama-Journal of the American Medical Association
Dual vs single protease inhibitor therapy following antiretroviral treatment failure - A randomized trial
Hammer, SM; Vaida, F; Bennett, KK; Holohan, MK; Sheiner, L; Eron, JJ; Wheat, LJ; Mitsuyasu, RT; Gulick, RM; Valentine, FT; Aberg, JA; Rogers, MD; Karol, CN; Saah, AJ; Lewis, RH; Bessen, LJ; Brosgart, C; DeGruttola, V; Mellors, JW
Jama-Journal of the American Medical Association, 288(2): 169-180.

Clinical Infectious Diseases
Antiretroviral drug resistance testing in adults infected with human immunodeficiency virus type 1: 2003 recommendations of an international AIDS Society-USA panel
Hirsch, MS; Brun-Vezinet, F; Clotet, B; Conway, B; Kuritzkes, DR; D'Aquila, RT; Demeter, LM; Hammer, SM; Johnson, VA; Loveday, C; Mellors, JW; Jacobsen, DM; Richman, DD
Clinical Infectious Diseases, 37(1): 113-128.

Hiv Medicine
British HIV Association (BHIVA) guidelines for the treatment of HIV-infected adults with antiretroviral therapy (2005)
Gazzard, B
Hiv Medicine, 6(): 1-61.

Enfermedades Infecciosas Y Microbiologia Clinica
Net benefits of resistance testing directed therapy compared with standard of care in HIV-infected patients with virological failure: A meta-analysis
Ena, J; de Apodaca, RFR; Amador, C; Benito, C; Pasquau, F
Enfermedades Infecciosas Y Microbiologia Clinica, 24(4): 232-237.

Journal of Korean Medical Science
Antiretroviral Genotypic Resistance Mutations in HIV-1 Infected Korean Patients with Virologic Failure
Chin, BS; Choi, JY; Choi, JY; Kim, GJ; Kee, MK; Kim, JM; Kim, SS
Journal of Korean Medical Science, 24(6): 1031-1037.
10.3346/jkms.2009.24.6.1031
CrossRef
Antiviral Therapy
Low genetic barrier to large increases in HIV-1 cross-resistance to protease inhibitors during salvage therapy
Morand-Joubert, L; Charpentier, C; Poizat, G; Chene, G; Dam, E; Raguin, G; Taburet, AM; Girard, PM; Hance, AJ; Clavel, F
Antiviral Therapy, 11(2): 143-154.

Labmedicine
Clinical significance of HIV-1 drug resistance mutations
Wagner, TA; Frenkel, LM
Labmedicine, 37(9): 554-561.
10.1309/UCH8A9GR5KAO1VPU
CrossRef
Lancet Infectious Diseases
Clinical pharmacodynamics of HIV-1 protease inhibitors: use of inhibitory quotients to optimise pharmacotherapy
Morse, GD; Catanzaro, LM; Acosta, EP
Lancet Infectious Diseases, 6(4): 215-225.

Journal of Virological Methods
Prediction of HIV-1 drug susceptibility phenotype from the viral genotype using linear regression modeling
Vermeiren, H; Van Craenenbroeck, E; Alen, P; Bacheler, L; Picchio, G; Lecocq, P
Journal of Virological Methods, 145(1): 47-55.
10.1016/j.jviromet.2007.05.009
CrossRef
Antiviral Research
Running a tightrope: Regulatory challenges in the development of antiretrovirals
Naeger, LK; Struble, KA; Muffay, JS; Birnkrant, DB
Antiviral Research, 85(1): 232-240.
10.1016/j.antiviral.2009.07.016
CrossRef
Antiviral Therapy
The NIQ of lopinavir is predictive of a 48-week virological response in highly treatment-experienced HIV-1-infected subjects treated with a lop inavir/ritonavir-containing regimen
Castagna, A; Gianotti, N; Galli, L; Danise, A; Hasson, H; Boerr, E; Hoetelmans, R; Nauwelaers, D; Lazzarin, A
Antiviral Therapy, 9(4): 537-543.

Hiv Clinical Trials
A randomized controlled trial of the clinical utility of genotypic resistance testing in patients with limited prior exposure to antiretroviral drugs
Babiker, AG; Breckenridge, A; Darbyshire, JH; Dunn, DT; Fakoya, A; Fisher, M; Brown, AL; Loveday, C; McCormack, S; Pillay, D; Poppa, A; Rinehart, A; Verbiest, W; Williams, I
Hiv Clinical Trials, 6(4): 183-186.

Hiv Clinical Trials
Non-nucleoside phenotypic hypersusceptibility cut-point determination from ACTG 359
Haubrich, RH; Jiang, HY; Swanstrom, R; Bates, M; Katzenstein, D; Petch, L; Fletcher, CV; Fiscus, SA; Gulick, RM
Hiv Clinical Trials, 8(2): 63-67.
10.1310/hct0802-63
CrossRef
Hiv Clinical Trials
Evolution of genotypic resistance algorithms and their impact on the interpretation of clinical trials: An OPTIMA trial substudy
Desai, S; Kyriakides, T; Holodniy, M; Al-Salman, J; Griffith, B; Kozal, M
Hiv Clinical Trials, 8(5): 293-302.
10.1310/hct0805-293
CrossRef
Brazilian Journal of Medical and Biological Research
Evaluation of genotype resistance testing for salvage antiretroviral therapy at AIDS care centers from Ribeirao Preto, Sao Paulo, Brazil
Neto, RJP; Colares, JKB; Fonseca, BAL
Brazilian Journal of Medical and Biological Research, 41(6): 533-538.

Infection
SIMIT guidelines for the diagnosis and treatment of HIV infection
[Anon]
Infection, 36(5): 497-508.

Journal of Virological Methods
Clinical cut-offs for HIV-1 phenotypic resistance estimates: Update based on recent pivotal clinical trial data and a revised approach to viral mixtures
Winters, B; Van Craenenbroeck, E; Van der Borght, K; Lecocq, P; Villacian, J; Bacheler, L
Journal of Virological Methods, 162(): 101-108.
10.1016/j.jviromet.2009.07.023
CrossRef
AIDS
Limited benefit of antiretrovial resistance testing in treatment-experienced patients: a meta-analysis
Panidou, ET; Trikalinos, TA; Ioannidis, JPA
AIDS, 18(): 2153-2161.

Current Issues in Molecular Biology
Molecular diagnosis of medical viruses
Ratcliff, RM; Chang, G; Kok, T; Sloots, TP
Current Issues in Molecular Biology, 9(): 87-102.

Journal of Virological Methods
Systematic evaluation of allele-specific real-time PCR for the detection of minor HIV-1 variants with pol and env resistance mutations
Paredes, R; Marconi, VC; Campbell, TB; Kuritzkes, DR
Journal of Virological Methods, 146(): 136-146.
10.1016/j.jviromet.2007.06.012
CrossRef
Scandinavian Journal of Infectious Diseases
Theme 11 - Too early or too late: A never-ending dilemma with new technologies
Biondi, G
Scandinavian Journal of Infectious Diseases, 35(): 99-104.
10.1080/03008870310016247
CrossRef
Expert Review of Molecular Diagnostics
Bioinformatics approach to predicting HIV drug resistance
Cordes, F; Kaiser, R; Selbig, J
Expert Review of Molecular Diagnostics, 6(2): 207-215.
10.1586/14737159.6.2.207
CrossRef
Journal of Medical Virology
Retrospective analysis of antiretroviral HIV treatment success based on medical history or guided by the reverse hybridisation LiPA HIV genotyping system
Alvarez, M; Garcia, F; Martinez, NM; Quero, JH; Louwagie, J; De Brauwer, A; Maroto, MC
Journal of Medical Virology, 73(2): 151-157.
10.1002/jmv.20069
CrossRef
Clinics in Laboratory Medicine
Emerging viral infections
Su, JR
Clinics in Laboratory Medicine, 24(3): 773-+.
10.1016/j.cll.2004.05.002
CrossRef
Journal of Clinical Microbiology
Complementation in cells cotransfected with a mixture of wild-type and mutant human immunodeficiency virus (HIV) influences the replication capacities and phenotypes of mutant variants in a single-cycle HIV resistance assay
Mo, HM; Lu, LJ; Pithawalla, R; Kempf, DJ; Molla, A
Journal of Clinical Microbiology, 42(9): 4169-4174.
10.1128/JCM.42.9.4169-4174.2004
CrossRef
AIDS Reviews
Interpreting resistance data for HIV-1 therapy management - Know the limitations
Van Laethem, K; Vandamme, AM
AIDS Reviews, 8(1): 37-43.

Lancet Infectious Diseases
Approach to salvage antiretroviral therapy in heavily anti retroviral-experienced HIV-positive adults
Temesgen, Z; Cainelli, F; Poeschla, EM; Vlahakis, SAR; Vento, S
Lancet Infectious Diseases, 6(8): 496-507.

American Journal of Pharmaceutical Education
The pharmacology of HIV drug resistance
Zdanowicz, MM
American Journal of Pharmaceutical Education, 70(5): -.
ARTN 100
CrossRef
Journal of Antimicrobial Chemotherapy
Stopping HIV fusion with enfuvirtide: the first step to extracellular HAART
Moyle, G
Journal of Antimicrobial Chemotherapy, 51(2): 213-217.
10.1093/jac/dkg066
CrossRef
Antiviral Therapy
Phenotype or virtual phenotype for choosing antiretroviral therapy after failure: a prospective, randomized study
Perez-Elias, MJ; Garcia-Arato, I; Munoz, V; Santos, I; Sanz, J; Abraira, V; Arribas, JR; Gonzalez, J; Moreno, A; Dronda, F; Antela, A; Pumares, M; Marti-Belda, P; Casado, JL; Geijo, P; Moreno, S
Antiviral Therapy, 8(6): 577-584.

Antiviral Therapy
Updated European recommendations for the clinical use of HIV drug resistance testing
Vandamme, AM; Sonnerborg, A; Ait-Khaled, M; Albert, J; Asjo, B; Bacheler, L; Banhegyi, D; Boucher, C; Brun-Vezinet, F; Camacho, R; Clevenbergh, P; Clumeck, N; Dedes, N; De Luca, A; Doerr, HW; Faudon, JL; Gatti, G; Gerstoft, J; Hall, WW; Hatzakis, A; Hellmann, N; Horban, A; Lundgren, JD; Kempf, D; Miller, M; Miller, V; Myers, TW; Nielsen, C; Opravil, M; Palmisano, L; Perno, CF; Phillips, A; Pillay, D; Pumarola, T; Ruiz, L; Salminen, M; Schapiro, J; Schmidt, B; Schmit, JC; Schuurman, R; Shulse, E; Soriano, V; Staszewski, S; Vella, S; Youle, M; Ziermann, R; Perrin, L
Antiviral Therapy, 9(6): 829-848.

AIDS Reviews
Predictors of virologic response to ritonavir-boosted protease inhibitors
Marcelin, AG; Flandre, P; Peytavin, G; Calvez, V
AIDS Reviews, 7(4): 225-232.

Deutsche Medizinische Wochenschrift
Antiretroviral therapy of HIV infection - German-Austrian recommendations
[Anon]
Deutsche Medizinische Wochenschrift, 134(): S4-S15.
10.1055/s-0028-1123965
CrossRef
Jaids-Journal of Acquired Immune Deficiency Syndromes
Limited evolution of HIV antiretroviral drug resistance-associated mutations during the performance of drug resistance testing
Birch, C; Middleton, T; Hales, G; Cooper, D; Law, M; Crowe, S; Hoy, J; Emery, S
Jaids-Journal of Acquired Immune Deficiency Syndromes, 32(1): 57-61.

Clinics in Laboratory Medicine
HIV-1 genotypic and phenotypic resistance
Hanna, GJ
Clinics in Laboratory Medicine, 22(3): 637-+.
PII S0272-2712(02)00007-0
CrossRef
European Journal of Medical Research
German-Austrian recommendations for the antiretroviral therapy of HIV-infection (Status May 2004)
Salzberger, B; Marcus, U; Vielhaber, B; Arasteh, K; Golz, J; Brockmeyer, NH; Rockstroh, J
European Journal of Medical Research, 9(): 491-504.

Antimicrobial Agents and Chemotherapy
Discordances between interpretation algorithms for genotypic resistance to protease and reverse transcriptase inhibitors of human immunodeficiency virus are subtype dependent
Snoeck, J; Kantor, R; Shafer, RW; Van Laethem, K; Deforche, K; Carvalho, AP; Wynhoven, B; Soares, MA; Cane, P; Clarke, J; Pillay, C; Sirivichayakul, S; Ariyoshi, K; Holguin, A; Rudich, H; Rodrigues, R; Bouzas, MB; Brun-Vezinet, F; Reid, C; Cahn, P; Brigido, LF; Grossman, Z; Soriano, V; Sugiura, W; Tanuri, A; Harrigan, RP; Camacho, R; Schapiro, JM; Katzenstein, D; Vandamme, AM
Antimicrobial Agents and Chemotherapy, 50(2): 694-701.
10.1128/AAC.50.2.694-701.2006
CrossRef
Journal of Virological Methods
A study of seven rule-based algorithms for the interpretation of HIV-1 genotypic resistance data in Thailand
Poonpiriya, V; Sungkanuparph, S; Leechanachai, P; Pasomsub, E; Watitpun, C; Chunhakan, S; Chantratita, W
Journal of Virological Methods, 151(1): 79-86.
10.1016/j.jviromet.2008.03.017
CrossRef
Antiviral Therapy
Salvage therapy with abacavir in HIV-1-infected patients with previously documented M184V mutation: a possibility of NRTI recycling
Maggiolo, F; Callegaro, A; Arici, C; Quinzan, G; Gregis, G; Ripamonti, D; Tebaldi, A; Goglio, A; Suter, F
Antiviral Therapy, 8(2): 121-126.

Clinical Infectious Diseases
Efficacy of cerebrospinal fluid (CSF)-penetrating antiretroviral drugs against HIV in the neurological compartment: Different patterns of phenotypic resistance in CSF and plasma
Antinori, A; Perno, CF; Giancola, ML; Forbici, F; Ippolito, G; Hoetelmans, RM; Piscitelli, SC
Clinical Infectious Diseases, 41(): 1787-1793.

Journal of Postgraduate Medicine
Antiretroviral drug resistance testing
Sen, S; Tripathy, SP; Paranjape, RS
Journal of Postgraduate Medicine, 52(3): 187-193.

AIDS Research and Human Retroviruses
A Comparison of Interpretation by Three Different HIV Type 1 Genotypic Drug Resistance Algorithms Using Sequences from Non-Clade B HIV Type 1 Strains
Kandathil, AJ; Kannangai, R; Abraham, OC; Pulimood, SA; Jensen, MA; Sridharan, G
AIDS Research and Human Retroviruses, 25(3): 315-318.
10.1089/aid.2008.0177
CrossRef
Clinical Microbiology Reviews
Genotypic testing for human immunodeficiency virus type 1 drug resistance
Shafer, RW
Clinical Microbiology Reviews, 15(2): 247-+.
10.1128/CMR.15.2.247-277.2002
CrossRef
Jama-Journal of the American Medical Association
Antiretroviral treatment for adult HIV infection in 2002 - Updated recommendations of the international AIDS Society-USA panel
Yeni, PG; Hammer, SM; Carpenter, CCJ; Cooper, DA; Fischl, MA; Gatell, JM; Gazzard, BG; Hirsch, MS; Jacobsen, DM; Katzenstein, DA; Montaner, JSG; Richman, DD; Saag, MS; Schechter, M; Schooley, RT; Thompson, MA; Vella, S; Volberding, PA
Jama-Journal of the American Medical Association, 288(2): 222-235.

European Journal of Medical Research
Antiretroviral therapy of HIV infection German-Austrian recommendations (July 2002)
Arasteh, K; Bader, A; Berg, T; Bogner, J; Brockhaus, W; Brockmeyer, NH; Brodt, HR; Busch, H; Doerr, HW; Dupke, S; Esser, S; Fleckenstein, B; Goebel, FD; Golz, J; Grosch-Worner, I; Gurtler, L; Hartmann, M; Helm, EB; Hoffmann, C; Jager, H; Jablonowski, H; Jarke, J; Kern, P; Knechten, H; Korner, T; Korn, K; Kramer, A; Krausslich, HG; Kurowski, M; Marcus, U; Moll, A; Pfeil, B; Plettenberg, A; Rasokat, H; Rieger, A; Rockstroh, J; Ruf, B; Rump, JA; Salzberger, B; Schafberger, A; Schedel, I; Schmied, B; Schmidt, B; Schmidt, RE; Schofer, H; Schrappe, M; Staszewski, S; Stellbrink, HJ; Stoehr, A; Stoll, M; Tschachler, E; Uberla, K; van Lunzen, J; Vetter, N; Vielhaber, B; Zangerle, R
European Journal of Medical Research, 8(6): 257-274.

Cancer Investigation
The role of surrogate markers in the clinical development of antiretroviral therapy: A model for early evaluation of targeted cancer drugs
Petrella, M; Montaner, J; Batist, G; Wainberg, MA
Cancer Investigation, 22(1): 149-160.
10.1081/CNV-120027590
CrossRef
Journal of Virology
Phenotypic hypersusceptibility to multiple protease inhibitors and low replicative capacity in patients who are chronically infected with human immunodeficiency virus type 1
Martinez-Picado, J; Wrin, T; Frost, SDW; Clotet, B; Ruiz, L; Brown, AJL; Petropouios, CJ; Parkin, NT
Journal of Virology, 79(): 5907-5913.

Clinical Drug Investigation
Cost-efficacy comparison among three antiretroviral regimens in HIV-1 infected, treatment-experienced patients
Ruof, J; Dusek, A; DeSpirito, M; DeMasi, RA
Clinical Drug Investigation, 27(7): 469-479.

Medicinal Research Reviews
Update on HIV resistance and resistance testing
Sebastian, J; Faruki, H
Medicinal Research Reviews, 24(1): 115-125.

Antiviral Therapy
Comparison of tests and procedures to build clinically relevant genotypic scores: application to the Jaguar study
Flandre, P; Marcelin, AG; Pavie, J; Shmidely, N; Wirden, M; Lada, O; Bernard, MC; Molina, JM; Calvez, V
Antiviral Therapy, 10(4): 479-487.

Clinical Infectious Diseases
Comparison of a rule-based algorithm with a phenotype-based algorithm for the interpretation of HIV genotypes in guiding salvage regimens in HIV-infected patients by a randomized clinical trial: The mutations and salvage study
Gianotti, N; Mondino, V; Rossi, MC; Chiesa, E; Mezzaroma, I; Ladisa, N; Guaraldi, G; Torti, C; Tarquini, P; Castelli, P; Di Carlo, A; Boeri, E; Keulen, W; Mc Kenna, P; Lazzarin, A
Clinical Infectious Diseases, 42(): 1470-1480.

Archives of Pharmacal Research
Antiretroviral therapy 2006: Pharmacology, applications, and special situations
Samuel, R; Bettiker, R; Suh, B
Archives of Pharmacal Research, 29(6): 431-458.

Chinese Medical Journal
Phenotypic resistance of resistant strains of HIV type-1 subtype B in China
Li, J; Lu, JF; Dong, HH; Bao, ZY; Liu, SY; Li, HP; Zhuang, DM; Liu, YJ; Li, H; Wang, Z; Wu, H; Li, JY
Chinese Medical Journal, 119(): 1972-1977.

Pediatrics
Tenofovir disoproxil fumarate and an optimized background regimen of antiretroviral agents as salvage therapy for pediatric HIV infection
Hazra, R; Gafni, RI; Maldarelli, F; Balis, FM; Tullio, AN; DeCarlo, E; Worrell, CJ; Steinberg, SM; Flaherty, J; Yale, K; Kearney, BP; Zeichner, SL
Pediatrics, 116(6): E846-E854.
10.1542/peds.2005-0975
CrossRef
Enfermedades Infecciosas Y Microbiologia Clinica
Clinical use of HIV-1 resistance genotyping. Predictive factors of poor virological evolution in salvage treatments
Riera-Jaume, M; Penaranda-Vera, M; Ribas-Blanco, MA; Murillas-Angoiti, J; Campins, A; Salas-Aparicio, A; Leyes-Garcia, M; Pareja-Bezares, A; Perez, JL; Villalonga-Pieras, C
Enfermedades Infecciosas Y Microbiologia Clinica, 24(4): 225-231.

Antiviral Therapy
HIV genotypic resistance testing to optimize antiretroviral prescribing: is there room for improvement?
Uy, J; Brooks, JT; Baker, R; Hoffman, M; Moorman, A; Novak, R
Antiviral Therapy, 12(6): 957-962.

Journal of Infectious Diseases
Improved interpretation of genotypic changes in the HIV-1 reverse transcriptase coding region that determine the virological response to didanosine
De Luca, A; Di Giambenedetto, S; Trotta, MP; Colafigli, M; Prosperi, M; Ruiz, L; Baxter, J; Clevenbergh, P; Cauda, R; Perno, CF; Antinori, A
Journal of Infectious Diseases, 196(): 1645-1653.
10.1086/522231
CrossRef
New England Journal of Medicine
HIV drug resistance - A chink in the armor
Hirsch, MS
New England Journal of Medicine, 347(6): 438-439.

Journal of Clinical Virology
Drug resistance in non-subtype BHIV-1
Kantor, R; Katzenstein, D
Journal of Clinical Virology, 29(3): 152-159.
10.1016/S1386-6532(03)00115-X
CrossRef
New England Journal of Medicine
Structured treatment interruption in patients with multidrug-resistant human immunodeficiency virus
Lawrence, J; Mayers, DL; Hullsiek, KH; Collins, G; Abrams, DI; Reisler, RB; Crane, LR; Schmetter, BS; Dionne, TJ; Saldanha, JM; Jones, MC; Baxter, JD
New England Journal of Medicine, 349(9): 837-846.

Apmis
Molecular biological assessment methods and understanding the course of the HIV infection
Katzenstein, TL
Apmis, 111(): 5-37.

Antimicrobial Agents and Chemotherapy
Natural variation of drug susceptibility in wild-type human immunodeficiency virus type 1
Parkin, NT; Hellmann, NS; Whitcomb, JM; Kiss, L; Chappey, C; Petropoulos, CJ
Antimicrobial Agents and Chemotherapy, 48(2): 437-443.
10.1128/AAC.48.2.437-443.2004
CrossRef
Clinical Infectious Diseases
Long-term efficacy of routine access to antiretroviral-resistance testing in HIV type 1-infected patients: Results of the clinical efficacy of resistance testing trial
Wegner, SA; Wallace, MR; Aronson, NE; Tasker, SA; Blazes, DL; Tamminga, C; Fraser, S; Dolan, MJ; Stephan, KT; Michael, NL; Jagodzinski, LL; Vahey, MT; Gilcrest, JL; Tracy, L; Milazzo, MJ; Murphy, DJ; McKenna, P; Hertogs, K; Rinehart, A; Larder, B; Birx, DL
Clinical Infectious Diseases, 38(5): 723-730.

Antiviral Therapy
Impact of genotypic resistance testing on selection of salvage regimen in clinical practice
Haupts, S; Ledergerber, B; Boni, J; Schupbach, J; Kronenberg, A; Opravil, M; Flepp, M; Speck, RF; Grube, C; Rentsch, K; Weber, R; Gunthard, HF
Antiviral Therapy, 8(5): 443-454.

Antiviral Therapy
HIV drug resistance testing: is the evidence really there?
Dunn, DT; Gibb, DM; Babiker, AG; Green, H; Darbyshire, JH; Weller, IVD
Antiviral Therapy, 9(5): 641-648.

Cellular and Molecular Life Sciences
Overcoming HIV drug resistance through rational drug design based on molecular, biochemical, and structural profiles of HIV resistance
Yin, PD; Das, D; Mitsuya, H
Cellular and Molecular Life Sciences, 63(): 1706-1724.
10.1007/s00018-006-6009-7
CrossRef
Antiviral Research
Algorithms for the interpretation of HIV-1 genotypic drug resistance information
Vercauteren, J; Vandamme, AM
Antiviral Research, 71(): 335-342.
10.1016/j.antiviral.2006.05.003
CrossRef
Antiviral Therapy
Advantages of predicted phenotypes and statistical learning models in inferring virological response to antiretroviral therapy from HIV genotype
Altmann, A; Sing, T; Vermeiren, H; Winters, B; Van Craenenbroeck, E; Van der Borght, K; Rhee, SY; Shafer, RW; Schulter, E; Kaiser, R; Peres, Y; Sonnerborg, A; Fessel, WJ; Incardona, F; Zazzi, M; Bacheler, L; Van Vlijmen, H; Lengauer, T
Antiviral Therapy, 14(2): 273-283.

Journal of Infectious Diseases
Comparison between rules-based human immunodeficiency virus type 1 genotype interpretations and real or virtual phenotype: Concordance analysis and correlation with clinical outcome in heavily treated patients
Torti, C; Quiros-Roldan, E; Keulen, W; Scudeller, L; Lo Caputo, S; Boucher, C; Castelli, F; Mazzotta, F; Pierotti, P; Been-Tiktak, AM; Buccoliero, G; De Gennaro, M; Carosi, G; Tinelli, C
Journal of Infectious Diseases, 188(2): 194-201.

Clinical Infectious Diseases
Workshop on HIV infection and aging: What is known and future research directions
Effros, RB; Fletcher, CV; Gebo, K; Halter, JB; Hazzard, WR; Horne, FM; Huebner, RE; Janoff, EN; Justice, AC; Kuritzkes, D; Nayfield, SG; Plaeger, SF; Schmader, KE; Ashworth, JR; Campanelli, C; Clayton, CP; Rada, B; Woolard, NF; High, KP
Clinical Infectious Diseases, 47(4): 542-553.
10.1086/590150
CrossRef
Hiv Medicine
Prediction of phenotypic susceptibility to antiretroviral drugs using physiochemical properties of the primary enzymatic structure combined with artificial neural networks
Kjer, J; Hoj, L; Fox, Z; Lundgren, JD
Hiv Medicine, 9(8): 642-652.
10.1111/j.1468-1293.2008.00612.x
CrossRef
Journal of Medical Virology
The Relationship Between Genotypic Sensitivity Score and Treatment Outcomes in Late Stage HIV Disease After Supervised HAART
Castor, D; Vlahov, D; Hoover, DR; Berkman, A; Wu, YF; Zeller, B; Brechtl, J; Hammer, SM
Journal of Medical Virology, 81(8): 1323-1335.
10.1002/jmv.21500
CrossRef
Enfermedades Infecciosas Y Microbiologia Clinica
Spanish GESIDA/National AIDS Plan recommendations for antiretroviral therapy in HIV-infected adults in the year 2002
Rubio, R; Berenguer, J; Miro, JM; Antela, A; Iribarren, JA; Gonzalez, J; Guerra, L; Moreno, S; Arrizabalaga, J; Clotet, B; Gatell, JM; Laguna, F; Martinez, E; Parras, F; Santamaria, JM; Tuset, M; Viciana, P
Enfermedades Infecciosas Y Microbiologia Clinica, 20(6): 244-303.

Deutsche Medizinische Wochenschrift
Antiretroviral therapy of HIV infection. German-Austrian guidelines (July 2002)
Brockmeyer, NH
Deutsche Medizinische Wochenschrift, 128(): S7-S18.

Current Opinion in Molecular Therapeutics
The role of phenotyping and replication 2 capacity in anti-HIV therapeutics
Baliga, CS; Sutton, RE
Current Opinion in Molecular Therapeutics, 6(3): 308-317.

Journal of Clinical Virology
Virologic therapy response significantly correlates with the number of active drugs as evaluated using a LiPA HIV-1 resistance scoring system
Ziermann, R; Celis, L; Derdelinckx, I; Lambert, C; Veeck, J; Rizzo, MG; Vanderborght, B; Zissis, G; Clumeck, N; Fransen, K; Vaira, D; Hendricks, D; Van Laethem, K; Vandamme, AM; Schmit, JC; Knechten, H; De Luca, A; Louwagie, J; Segers, P; De Boeck, K; Pottel, H; De Brauwer, A; Hulstaert, F
Journal of Clinical Virology, 31(): S7-S15.
10.1016/j.jcv.2004.09.014
CrossRef
Antiviral Therapy
Clinically validated genotype analysis: guiding principles and statistical concerns
Brun-Vezinet, F; Costagliolo, D; Khaled, MA; Calvez, V; Clavel, F; Clotet, B; Haubrich, R; Kemp, D; King, M; Kuritzkes, D; Lanier, R; Miller, M; Miller, V; Phillips, A; Pillay, D; Schapiro, J; Scott, J; Shafer, R; Zazzi, M; Zolopa, A; DeGruttola, V
Antiviral Therapy, 9(4): 465-478.

Antiviral Therapy
Current status and future prospects of therapeutic drug monitoring and applied clinical pharmacology in antiretroviral therapy
Boffito, M; Acosta, E; Burger, D; Fletcher, CV; Flexner, C; Garaffo, R; Gatti, G; Kurowski, M; Perno, CF; Peytavin, G; Regazzi, M; Back, D
Antiviral Therapy, 10(3): 375-392.

European Journal of Clinical Microbiology & Infectious Diseases
Genotypic and phenotypic resistance testing of HIV-1 in routine clinical care
Hirsch, HH; Drechsler, H; Holbro, A; Hamy, F; Sendi, P; Petrovic, K; Klimkait, T; Battegay, M
European Journal of Clinical Microbiology & Infectious Diseases, 24(): 733-738.
10.1007/s10096-005-0044-4
CrossRef
Antiviral Therapy
A randomized controlled trial of genotypic HIV drug resistance testing in HIV-1-infected children: The PERA (PENTA 8) trial
Green, H; Gibb, DM; Compagnucci, A; Giacomet, V; de Rossi, A; Harper, L; Saidi, Y; Castelli-Gattinara, G; Pillay, D; Babiker, AG; Aboulker, JP; Lyall, H; T Bacheler, L; Walker, AS; Debre, M; Rosso, R; Burger, DM; Della Negra, M; David, DT; Giaquinto, C
Antiviral Therapy, 11(7): 857-867.

Journal of Infectious Diseases
Managing antiretroviral therapy: Changing regimens, resistance testing, and the risks from structured treatment interruptions
Eron, JJ
Journal of Infectious Diseases, 197(): S261-S271.
10.1086/533418
CrossRef
Clinics in Laboratory Medicine
Detection of antiretroviral resistance in HIV-1
Cavert, W; Balfour, HH
Clinics in Laboratory Medicine, 23(4): 915-+.
10.1016/S0272-2712(03)00083-0
CrossRef
Journal of Virological Methods
Correlation between rules-based interpretation and virtual phenotype interpretation of HIV-1 genotypes for predicting drug resistance in HIV-infected individuals
Gallego, O; Martin-Carbonero, L; Aguero, J; de Mendoza, C; Corral, A; Soriano, V
Journal of Virological Methods, 121(1): 115-118.
10.1016/j.jviromet.2004.06.003
CrossRef
Antiviral Therapy
Nucleoside and nucleotide analogue reverse transcriptase inhibitors: a clinical review of antiretroviral resistance
Gallant, JE; Gerondelis, PZ; Wainberg, MA; Shulman, NS; Haubrich, RH; St Clair, M; Lanier, ER; Hellmann, NS; Richman, DD
Antiviral Therapy, 8(6): 489-506.

Antiviral Therapy
Variability in the interpretation of transmitted genotypic HIV-1 drug resistance and prediction of virological outcomes of the initial HAART by distinct systems
De Luca, A; Cozzi-Lepri, A; Perno, CF; Balotta, C; Di Giambenedetto, S; Poggio, A; Pagano, G; Tositti, G; Piscopo, R; Del Forno, A; Chiodo, F; Magnanio, G; Monforte, AD
Antiviral Therapy, 9(5): 743-752.

Journal of Clinical Microbiology
Use of new T-cell-based cell lines expressing two luciferase reporters for accurately evaluating susceptibility to anti-human immunodeficiency virus type 1 drugs
Chiba-Mizutani, T; Miura, H; Matsuda, M; Matsuda, Z; Yokomaku, Y; Miyauchi, K; Nishizawa, M; Yamamoto, N; Sugiura, W
Journal of Clinical Microbiology, 45(2): 477-487.
10.1128/JCM.01708-06
CrossRef
Journal of Clinical Microbiology
Comparison of oligonucleotide ligation assay and consensus sequencing for detection of drug-resistant mutants of human immunodeficiency virus type 1 in peripheral blood mononuclear cells and plasma
Ellis, GM; Mahalanabis, M; Beck, IA; Pepper, G; Wright, A; Hamilton, S; Holte, S; Naugler, WE; Pawluk, DM; Li, CC; Frenkel, LM
Journal of Clinical Microbiology, 42(8): 3670-3674.
10.1128/jcm.42.8.3670-3674.2004
CrossRef
International Journal of Std & AIDS
Genotypic resistance testing in HIV-infected pregnant women in an urban setting
Shah, SS; Crane, M; Monaghan, K; McGowan, JP
International Journal of Std & AIDS, 15(6): 384-387.

AIDS Reviews
Strategies for overcoming resistance in HIV-1 infected patients receiving HAART
Clotet, B
AIDS Reviews, 6(3): 123-130.

Antiviral Therapy
HIV-1 subtypes and response to combination antiretroviral therapy in Europe
Bannister, WP; Ruiz, L; Loveday, C; Vella, S; Zilmer, K; Kjaer, J; Knysz, B; Phillips, AN; Mocroft, A; Lundgren, JD
Antiviral Therapy, 11(6): 707-715.

Infectious Disease Clinics of North America
Approach to the treatment-experienced patient
Gallant, JE
Infectious Disease Clinics of North America, 21(1): 85-+.
10.1016/j.idc.2007.01.003
CrossRef
Journal of Infectious Diseases
Variable prediction of antiretroviral treatment outcome by different systems for interpreting genotypic human immunodeficiency virus type I drug resistance
De Luca, A; Cingolani, A; Di Giambenedetto, S; Trotta, MP; Baldini, F; Rizzo, MG; Bertoli, A; Liuzzi, G; Narciso, P; Murri, R; Antmassari, A; Perno, CF; Antinori, A
Journal of Infectious Diseases, 187(): 1934-1943.

Journal of Virological Methods
"All-in-One Assay", a direct phenotypic anti-human immunodeficiency virus type 1 drug resistance assay for three-drug combination therapies that takes into consideration in vivo drug concentrations
Hachiya, A; Matsuoka-Aizawa, S; Tsuchiya, K; Gatanaga, H; Kimura, S; Tatsumi, M; Oka, S
Journal of Virological Methods, 111(1): 43-53.
10.1016/S0166-0934(03)00150-2
CrossRef
Current Hiv Research
Susceptibility of HIV-1 to Tipranavir and Other Antiretroviral Agents in Treatment-Experienced Patients: The UTILIZE Study
Baxter, JD; Bhatti, L; Coakley, E; Bartczak, J; McDonough, M; Vinisko, R; Piliero, PJ
Current Hiv Research, 8(4): 347-354.

Antimicrobial Agents and Chemotherapy
Activities of atazanavir (BMS-232632) against a large panel of human immunodeficiency virus type 1 clinical isolates resistant to one or more approved protease inhibitors
Colonno, RJ; Thiry, A; Limoli, K; Parkin, N
Antimicrobial Agents and Chemotherapy, 47(4): 1324-1333.
10.1128/AAC.47.4.1324-1333.2003
CrossRef
AIDS
Efficacy of indinavir-ritonavir-based regimens in HIV-1-infected patients with prior protease inhibitor failures
Campo, RE; Moreno, JN; Suarez, G; Miller, N; Kolber, MA; Holder, DJ; Shivaprakash, M; DeAngelis, DM; Wright, JL; Schleif, WA
AIDS, 17(): 1933-1939.
10.1097/01.aids.0000076300.76477.92
CrossRef
Scandinavian Journal of Infectious Diseases
Theme 1 - Genotypic resistance tests in the management of the HIV-infected patient at virological failure
Aceti, A; Carosi, G; Torti, C
Scandinavian Journal of Infectious Diseases, 35(): 61-66.
10.1080/03008870310009740
CrossRef
AIDS Patient Care and Stds
Cross-resistance patterns among HIV protease inhibitors
Kozal, M
AIDS Patient Care and Stds, 18(4): 199-208.

Journal of Infectious Diseases
Weighted phenotypic susceptibility scores are predictive of the HIV-1 RNA response in protease inhibitor-experienced HIV-1-infected subjects
Swanstrom, R; Bosch, RJ; Katzenstein, D; Cheng, HL; Jiang, HY; Hellmann, N; Haubrich, R; Fiscus, SA; Fletcher, CV; Acosta, EP; Gulick, RM
Journal of Infectious Diseases, 190(5): 886-893.

Medecine Et Maladies Infectieuses
Therapeutic indications, optimization of antiretroviral treatment and quality of life of patients receiving enfuvirtide
Raffi, F
Medecine Et Maladies Infectieuses, 34(): 18-24.

Enfermedades Infecciosas Y Microbiologia Clinica
Spanish GESIDA/Nacional AIDS plan recommendations for antiretroviral therapy in HIV-infected adults (October 2004)
Iribarren, JA; Labarga, P; Rubio, R; Berenguer, J; Miro, JM; Antela, A; Gonzalez, J; Moreno, S; Arrizabalaga, J; Chamorro, L; Clotet, B; Gatell, JM; Lopez-Aldeguer, J; Martinez, E; Polo, R; Tuset, M; Viciana, P; Santamaria, JM; Kindelan, JM; Ribera, E; Segura, F
Enfermedades Infecciosas Y Microbiologia Clinica, 22(): 564-642.

Antiviral Therapy
Salvage therapy with amprenavir, lopinavir and ritonavir 200 mg/d or 400 mg/d in HIV-infected patients in virological failure
Raguin, G; Chene, G; Morand-Joubert, L; Taburet, AM; Droz, C; Le Tiec, C; Clavel, F; Girard, PM
Antiviral Therapy, 9(4): 615-625.

Antiviral Therapy
Baseline resistance and virological outcome in patients with virological failure who start a regimen containing abacavir: EuroSIDA study
Cabrera, C; Cozzi-Lepri, A; Phillips, AN; Loveday, C; Kirk, O; Ait-Khaled, M; Reiss, P; Kjaer, J; Ledergerber, B; Lundgren, JD; Clotet, B; Ruiz, L
Antiviral Therapy, 9(5): 787-800.

Advanced Drug Delivery Reviews
Dynamical models of biomarkers and clinical progression for personalized medicine: The HIV context
Prague, M; Commenges, D; Thiebaut, R
Advanced Drug Delivery Reviews, 65(7): 954-965.
10.1016/j.addr.2013.04.004
CrossRef
AIDS
Benefit of treatment interruption in HIV-infected patients with multiple therapeutic failures: a randomized controlled trial (ANRS 097)
Katlama, C; Dominguez, S; Gourlain, K; Duvivier, C; Delaugerre, C; Legrand, M; Tubiana, R; Reynes, J; Molina, J; Peytavin, G; Calvez, V; Costagliola, D
AIDS, 18(2): 217-226.

PDF (128)
AIDS
Phenotypic susceptibility and virological outcome in nucleoside-experienced patients receiving three or four antiretroviral drugs
for the ACTG 364 Study Team, ; Katzenstein, DA; Bosch, RJ; Hellmann, N; Wang, N; Bacheler, L; Albrecht, MA
AIDS, 17(6): 821-830.

PDF (218)
Current Opinion in Infectious Diseases
Finally, the new drug classes arrive
Moyle, G
Current Opinion in Infectious Diseases, 16(1): 1-3.

PDF (50)
Therapeutic Drug Monitoring
Understanding HIV-1 Drug Resistance
Frenkel, LM; Tobin, NH
Therapeutic Drug Monitoring, 26(2): 116-121.

PDF (81)
JAIDS Journal of Acquired Immune Deficiency Syndromes
Real Versus Virtual Phenotype to Guide Treatment in Heavily Pretreated Patients: 48-Week Follow-Up of the Genotipo-Fenotipo di Resistenza (GenPheRex) Trial
Lazzarin, A; Angarano, G; Maserati, R; Gianotti, N; Ladisa, N; Quiros-Roldan, E; Rinehart, AR; Mazzotta, F; Lo Caputo, S; Torti, C; Tinelli, C; Pierotti, P; Castelli, F; Carosi, G; for the Genotipo-Fenotipo di Resistenza (GenPheRex) Group of the Italian Management Standardizzato di Terapia Antiretrovirale (MASTER) Cohort,
JAIDS Journal of Acquired Immune Deficiency Syndromes, 32(3): 268-280.

PDF (8593)
JAIDS Journal of Acquired Immune Deficiency Syndromes
Determination of Clinically Relevant Cutoffs for HIV-1 Phenotypic Resistance Estimates Through a Combined Analysis of Clinical Trial and Cohort Data
Winters, B; Montaner, J; Harrigan, PR; Gazzard, B; Pozniak, A; Miller, MD; Emery, S; van Leth, F; Robinson, P; Baxter, JD; Perez-Elias, M; Castor, D; Hammer, S; Rinehart, A; Vermeiren, H; Van Craenenbroeck, E; Bacheler, L
JAIDS Journal of Acquired Immune Deficiency Syndromes, 48(1): 26-34.
10.1097/QAI.0b013e31816d9bf4
PDF (1101) | CrossRef
JAIDS Journal of Acquired Immune Deficiency Syndromes
HIV-1 Phenotypic Susceptibility to Lopinavir (LPV) and Genotypic Analysis in LPV/r-Naive Subjects With Prior Protease Inhibitor Experience
Monno, L; Saracino, A; Scudeller, L; Pastore, G; Bonora, S; Cargnel, A; Carosi, G; Angarano, G
JAIDS Journal of Acquired Immune Deficiency Syndromes, 33(4): 439-447.

PDF (5702)
JAIDS Journal of Acquired Immune Deficiency Syndromes
Selection of Antiretroviral Therapy Guided by Genotypic or Phenotypic Resistance Testing: An Open-Label, Randomized, Multicenter Study (PhenGen)
Saracino, A; Monno, L; Locaputo, S; Torti, C; Scudeller, L; Ladisa, N; Antinori, A; Sighinolfi, L; Chirianni, A; Mazzotta, F; Carosi, G; Angarano, G
JAIDS Journal of Acquired Immune Deficiency Syndromes, 37(5): 1587-1598.

PDF (1044)
AIDS
A randomized, prospective study of phenotype susceptibility testing versus standard of care to manage antiretroviral therapy: CCTG 575
Haubrich, RH; Kemper, CA; Hellmann, NS; Keiser, PH; Witt, MD; Tilles, JG; Forthal, DN; Leedom, J; Leibowitz, M; McCutchan, JA; Richman, DD; the California Collaborative Treatment Group,
AIDS, 19(3): 295-302.

PDF (113)
AIDS
Antiretroviral therapies for treatment-experienced patients: current status and research challenges
Struble, K; Murray, J; Cheng, B; Gegeny, T; Miller, V; Gulick, R
AIDS, 19(8): 747-756.

PDF (125)
JAIDS Journal of Acquired Immune Deficiency Syndromes
A Randomized Controlled Trial of the Value of Phenotypic Testing in Addition to Genotypic Testing for HIV Drug Resistance: Evaluation of Resistance Assays (ERA) Trial Investigators

JAIDS Journal of Acquired Immune Deficiency Syndromes, 38(5): 553-559.

PDF (268)
Back to Top | Article Outline
Keywords:

Clinical trials; highly active antiretroviral therapy; HIV diagnostic tests; HIV drug resistance/resistance mutations; phenotypic resistance testing

© 2002 Lippincott Williams & Wilkins, Inc.

Login

Search for Similar Articles
You may search for similar articles that contain these same keywords or you may modify the keyword list to augment your search.