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Semen quality in HIV patients under stable antiretroviral therapy is impaired compared to WHO 2010 reference values and on sperm proteome level

Pilatz, Adriana; Discher, Thomasb; Lochnit, Guenterc; Wolf, Juliaa; Schuppe, Hans-Christiana; Schüttler, Christian G.d; Hossain, Hamide; Weidner, Wolfganga; Lohmeyer, Jürgenb; Diemer, Thorstena

doi: 10.1097/QAD.0000000000000161
Clinical Science: Concise Communications

Objectives: To investigate semen quality in HIV patients under stable antiretroviral therapy (ART) compared with WHO 2010 reference values and on the sperm proteome level.

Design: Between 2011 and 2013, we prospectively enrolled 116 HIV-positive men without hepatitis B or C co-infections from our outpatient department for infectious diseases.

Methods: Patients received a comprehensive andrological work-up. Complete semen analysis was performed according to WHO 2010 recommendations, with each semen variable of the study population being compared with the WHO reference group (n ∼ 2000). Correlation analysis was done to investigate the influence of HIV surrogate parameters on semen quality. Two-dimensional gel electrophoresis and subsequent protein identification was performed to determine any differences in the sperm protein composition of the 15 HIV-positive patients and that of 15 age-matched healthy men.

Results: Median values of all assessed semen parameters were within a normal range. However, for each semen variable, about 25% of patients had values below the fifth percentile of the WHO 2010 reference group. Disease-related parameters (CD4+ cell count, viral load, CDC stage, duration of disease, duration of ART, number and type of antiretroviral drugs) were not significantly correlated with any sperm parameter. Sperm proteome analysis identified 14 downregulated proteins associated with sperm motility and fertility.

Conclusion: This is the first study that compares all standard semen parameters in HIV-positive patients under ART to WHO 2010 reference values. It provides evidence of impaired conventional semen parameters and altered sperm protein composition. Finally, HIV surrogate parameters are not suitable for predicting semen quality.

aDepartment of Urology, Pediatric Urology and Andrology

bDepartment of Internal Medicine II

cSchool for Medicine, Institute of Biochemistry

dInstitute for Medical Virology

eInstitute for Medical Microbiology, Justus Liebig University Giessen, Giessen, Germany.

Correspondence to Adrian Pilatz, MD, Department of Urology, Pediatric Urology and Andrology, Justus Liebig University (Giessen), Rudolf-Buchheim-Strasse 7, 35392 Giessen, Germany. Tel: +49 641 985 56362; fax: +49 641 985 44519; e-mail: adrian.pilatz@chiru.med.uni-giessen.de

Received 9 September, 2013

Revised 19 November, 2013

Accepted 19 November, 2013

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© 2014 Lippincott Williams & Wilkins, Inc.