A comparison of methods for measuring rectal HIV levels suggests that HIV DNA resides in cells other than CD4+ T cells, including myeloid cells

Yukl, Steven A.a,b; Sinclair, Elizabethb; Somsouk, Mab; Hunt, Peter W.b; Epling, Lorrieb; Killian, Maudib; Girling, Valerieb; Li, Peilina; Havlir, Diane V.b; Deeks, Steven G.b; Wong, Joseph K.a,b; Hatano, Hiroyub

doi: 10.1097/QAD.0000000000000166
Research Letters

We compared different techniques for measuring gut HIV reservoirs and assessed for HIV in non-CD4+ T cells. HIV DNA levels were similar when measured from rectal biopsies and isolated rectal cells, while HIV RNA tended to be higher in rectal cells. HIV DNA levels in total rectal cells were greater than those predicted from levels in sorted CD4+ T cells, suggesting a reservoir in non-CD4+ T cells, and HIV DNA was detected in sorted myeloid cells (7/7 subjects).

aSan Francisco Veterans Affairs Medical Center

bUniversity of California, San Francisco (UCSF), California, USA.

Correspondence to Steven A. Yukl, San Francisco VA Medical Center, 4150 Clement St, 111W3, San Francisco, CA 94121, USA. Tel: +1 415 221 4810x3930; fax: +1 415 379 5600; e-mail: steven.yukl@ucsf.edu

Received 30 July, 2013

Revised 26 November, 2013

Accepted 26 November, 2013

© 2014 Lippincott Williams & Wilkins, Inc.