Skip Navigation LinksHome > January 14, 2014 - Volume 28 - Issue 2 > Exosomes from breast milk inhibit HIV-1 infection of dendrit...
AIDS:
doi: 10.1097/QAD.0000000000000159
Basic Science

Exosomes from breast milk inhibit HIV-1 infection of dendritic cells and subsequent viral transfer to CD4+ T cells

Näslund, Tanja I.a; Paquin-Proulx, Dominicb; Paredes, Patricia Torregrosaa; Vallhov, Helena; Sandberg, Johan K.b; Gabrielsson, Susannea

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Abstract

Objective:

To investigate whether exosomes derived from human breast milk or plasma confer protection against HIV-1 infection of monocyte-derived dendritic cells (MDDCs) and subsequent viral transfer to CD4+ T cells.

Design:

MDDCs were generated and milk and plasma-derived exosomes were isolated from healthy donors. To determine the capacity of exosomes to inhibit HIV-1 infection, MDDCs were preincubated with exosomes before exposure to HIV-1BaL. To investigate transfer of HIV-1 from MDDCs to CD4+ T cells, MDDCs preincubated with exosomes and HIV-1BaL were cocultured with allogeneic CD4+ T cells. To explore receptors used by MDDCs for binding of exosomes, blocking experiments were performed.

Methods:

Productive HIV-1 infection was analysed in MDDCs and CD4+ T cells by determining p24 expression by flow cytometry. Confocal microscopy and flow cytometry was used to investigate uptake of fluorescently labelled exosomes by MDDCs.

Results:

Milk exosomes, but not plasma exosomes, bind MDDCs via DC-SIGN inhibiting HIV-1 infection of MDDCs and subsequent viral transfer to CD4+ T cells.

Conclusion:

We propose that milk exosomes act as a novel protective factor against vertical transmission of HIV-1 by competing with HIV-1 for binding to DC-SIGN on MDDCs.

© 2014 Wolters Kluwer Health | Lippincott Williams & Wilkins

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