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AIDS:
doi: 10.1097/QAD.0000000000000113
Basic Science

Differential expression of the alternatively spliced OPRM1 isoform [mu]-opioid receptor-1K in HIV-infected individuals

Dever, Seth M.a; Costin, Blair N.a; Xu, Ruqiangb; El-Hage, Naziraa; Balinang, Joyceb; Samoshkin, Alexanderc; O’Brien, Megan A.a; McRae, MaryPeaced; Diatchenko, Ludac; Knapp, Pamela E.b,e; Hauser, Kurt F.a,e

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Abstract

Objective:

We previously examined the expression of specific C-terminal μ-opioid receptor (MOR) splice variants in human central nervous system cell types and HIV-infected brain tissue from individuals with neurocognitive impairment ± HIV encephalitis (HIVE). In the present study, we examined the N-terminal splice variant MOR-1K, which mediates excitatory cellular signaling.

Methods and results:

We found segregation of expression ranging from undetectable to seemingly exclusive across nervous system cell types compared to the pool of C-terminal MOR splice variants using the real-time polymerase chain reaction (RT-PCR). Expression of MOR-1K mRNA was also increased in HIV-infected individuals with combined neurocognitive impairment and HIVE compared with the other groups. MOR-1K expression correlated with the level of patient neurocognitive impairment, whereas the pool of C-terminal MOR splice variants did not. HIVE was also associated with increased expression of the inflammatory mediators MCP-1, MCP-2, and RANTES, but not the host HIV coreceptors CXCR4 and CCR5 or the CD4 receptor using qRT-PCR. Network analysis of microarray data from these same patients revealed filamin A (FLNA) as a possible interaction partner with MOR-1K, and FLNA gene expression was also found to be upregulated in HIVE using qRT-PCR. Overexpression of FLNA in HEK293 cells redistributed MOR-1K from intracellular compartments to the cell surface.

Conclusion:

These results suggest that HIVE, and neurocognitive impairment depending on its severity, are associated with enhanced MOR-1K signaling through both increased expression and trafficking to the cell surface, which may alter the contribution of MOR receptor isoforms and exacerbate the effects of MOR activation in neuroAIDS.

© 2013 Wolters Kluwer Health | Lippincott Williams & Wilkins

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