Skip Navigation LinksHome > September 10, 2013 - Volume 27 - Issue 14 > Comparison of HIV DNA and RNA in gut-associated lymphoid tis...
AIDS:
doi: 10.1097/QAD.0b013e328362692f
Clinical Science: Concise Communication

Comparison of HIV DNA and RNA in gut-associated lymphoid tissue of HIV-infected controllers and noncontrollers

Hatano, Hiroyua; Somsouk, Maa; Sinclair, Elizabetha; Harvill, Karaa; Gilman, Leea; Cohen, Michellea; Hoh, Rebeccaa; Hunt, Peter W.a; Martin, Jeffrey N.b; Wong, Joseph K.a,c; Deeks, Steven G.a; Yukl, Steven A.a,c

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Abstract

Objectives:

HIV-infected controllers have provided novel insights into mechanisms of viral control. We investigated the degree to which HIV DNA and RNA are present in gut-associated lymphoid tissue (GALT) of controllers.

Design:

Cross-sectional cohort study.

Methods:

Colorectal biopsy pieces were obtained from five untreated noncontrollers, five ART-suppressed patients, and nine untreated controllers.

Results:

Rectal HIV DNA was lower in controllers (median 496 copies/106 CD4+ T cells) than in untreated noncontrollers (117483 copies/106 CD4+ T cells, P = 0.001) and ART-suppressed patients (6116 copies/106 CD4+ T cells, P = 0.004). Similarly, rectal HIV RNA was lower in controllers (19 copies/106 CD4+ T cells) than in noncontrollers (15210 copies/106 CD4+ T cells, P = 0.001) and ART-suppressed patients (1625 copies/106 CD4+ T cells, P = 0.0599). Rectal HIV RNA/DNA ratios were not statistically different between the three groups.

Conclusion:

Despite being able to maintain very low plasma HIV RNA levels in the absence of antiretroviral therapy (ART), HIV-infected controllers have readily measurable levels of HIV DNA and RNA in GALT. As expected, controllers had lower rectal HIV DNA and RNA compared with untreated noncontrollers and ART-suppressed individuals. Compared with the mechanisms of ‘natural’ viral control of controllers, long-term ART does not reduce the total HIV reservoir to the level of controllers.

© 2013 Wolters Kluwer Health | Lippincott Williams & Wilkins

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