Institutional members access full text with Ovid®

HIV-1 and HIV-2 produce different amounts of 2-long terminal repeat circular DNA in vitro

Gueudin, Mariea; Braun, Joséphinea; Plantier, Jean-Christophea; Simon, Françoisa,b

doi: 10.1097/QAD.0b013e328319edab
Research Letters

We assessed HIV-1 and HIV-2 2-long terminal repeat (LTR) circular DNA production in peripheral blood mononuclear cells, MT4-CXCR4 cells and HeLa-CXCR4-CCR5 cells in vitro, relative to the respective total amounts of HIV DNA. Whatever the cell type, HIV-2 produced a smaller total amount of DNA than HIV-1 between 6 and 96 h; HIV-2 2-LTR DNA appeared later than HIV-1 2-LTR DNA, but rapidly became more abundant. This accumulation of HIV-2 2-LTR DNA points to less efficient host cell integration relative to HIV-1.

aLaboratoire de virologie UPRES EA 2656 – Laboratoire associé du centre de référence pour le VIH, CHU Charles Nicolle 76031 ROUEN Cedex, France

bService de Microbiologie – CHU Saint Louis, Paris, France.

Received 16 June, 2008

Revised 8 September, 2008

Accepted 16 September, 2008

Correspondence to Marie Gueudin, Laboratoire de Virologie, CHU Charles Nicolle, 1 rue de Germont, 76031 Rouen Cedex, France. Tel: +33 2 32 88 82 36; fax: +33 2 32 88 04 30; e-mail: Marie.Gueudin@chu-rouen.fr

© 2008 Lippincott Williams & Wilkins, Inc.