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Drug-resistance surveillance among newly HIV-1 diagnosed individuals in Buenos Aires, Argentina

Dilernia, Dario Aa; Lourtau, Leonardob; Gomez, Alejandro Ma; Ebenrstejin, Juanb; Toibaro, Javier Jb; Bautista, Christian Tc,†; Marone, Rubénd; Carobene, Mauricioa; Pampuro, Sandraa; Gomez-Carrillo, Manuela; Losso, Marcelo Hb; Salomón, Horacioa

doi: 10.1097/QAD.0b013e3280b07db1
Epidemiology and Social: CONCISE COMMUNICATIONS

Objective: Our objective was to estimate primary resistance in an urban setting in a developing country with a long history of antiretroviral delivery and high coverage levels.

Design: We carried out a resistance surveillance study according to WHO HIV-Resistance Guidelines.

Methods: Blood samples were collected from 323 drug-naive HIV-1 infected individuals diagnosed at two HIV voluntary counselling and testing centers in Buenos Aires. Viral-load, CD4 cell counts and detuned assays were performed on all samples. The pol gene was sequenced and the resistance profile determined. Phylogenetic analysis was performed by neighbor-joining trees and bootscanning analysis.

Results: We found that 12 (4.2%) of the 284 samples sequenced harbored primary resistance mutations, of which K103N, M41L and V108I were most prevalent. Phylogenetic analysis revealed evidence for the transmission of the K103N mutation among the drug-naive population. The proportion of recent infections identified by the detuned assay was 10.1%.

Conclusions: Levels of primary resistance in Buenos Aires are still low, despite a long history of ARV delivery and high coverage levels.

From the aNational Reference Center for AIDS, School of Medicine, University of Buenos Aires, Buenos Aires, Argentina

bServicio de Inmunocomprometidos, Hospital General de Agudos José María Ramos Mejía, Buenos Aires, Argentina

cAsociación Benéfica PRISMA, Lima, Peru

dNexo Non-Governmental Gay Men Organization, Buenos Aires, Argentina.

Present affiliation: US Military HIV Research Program at the Walter Reed Army Institute of Research, Rockville, Maryland, USA.

Received 14 July, 2006

Revised 13 December, 2006

Accepted 11 January, 2007

Correspondence to Dr. Horacio Salomón, National Reference Center for AIDS, Department of Microbiology, School of Medicine, University of Buenos Aires, Paraguay 2155 piso 11, C1121ABG - Buenos Aires, Argentina. E-mail: hsalomon@fmed.uba.ar

© 2007 Lippincott Williams & Wilkins, Inc.