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Hypoxic Training: Effect on Mitochondrial Function and Aerobic Performance in Hypoxia


Medicine & Science in Sports & Exercise: October 2014 - Volume 46 - Issue 10 - p 1936–1945
doi: 10.1249/MSS.0000000000000321
Basic Sciences

Purpose: The effects of hypoxic training on exercise performance remain controversial. Here, we tested the hypotheses that i) hypoxic training possesses ergogenic effects at sea level and altitude and ii) the benefits are primarily mediated by improved mitochondrial function of the skeletal muscle.

Methods: We determined aerobic performance (incremental test to exhaustion and time trial for a set amount of work) in moderately trained subjects undergoing 6 wk of endurance training (3–4 times per week, 60 min per session) in normoxia (placebo, n = 8) or normobaric hypoxia (FIO2 = 0.15, n = 9) using a double-blind and randomized design. Exercise tests were performed in normoxia and acute hypoxia (FIO2 = 0.15). Skeletal muscle mitochondrial respiratory capacities and electron coupling efficiencies were measured via high-resolution respirometry. Total hemoglobin mass was assessed by carbon monoxide rebreathing.

Results: Skeletal muscle respiratory capacity was not altered by training or hypoxia; however, electron coupling control respective to fat oxidation slightly diminished with hypoxic training. Hypoxic training did increase total hemoglobin mass more than the placebo (8.4% vs 3.3%, P = 0.02). In normoxia, hypoxic training had no additive effect on maximal measures of oxygen uptake or time trial performance. In acute hypoxia, hypoxic training conferred no advantage on maximal oxygen uptake but tended to enhance time trial performance more than normoxic training (52% vs 32%, P = 0.09).

Conclusions: Our data suggest that, in moderately trained subjects, 6 wk of hypoxic training possesses no ergogenic effect at sea level. It is not excluded that hypoxic training might facilitate endurance capacity at moderate altitude; however, this issue is still open and needs to be further examined.

1Ecole Nationale des Sports de Montagne, site de l’Ecole Nationale de Ski et d’Alpinisme, Chamonix, FRANCE; 2Department of Exercise and Sport Sciences, University of Copenhagen, Copenhagen, DENMARK; 3Zürich Center for Integrative Human Physiology, University of Zürich, Zürich, SWITZERLAND; 4Institute of Physiology, University of Zürich, Zürich, SWITZERLAND; and 5Exercise Physiology, Institute of Human Movement Sciences, Eidgenössische Technische Hochschule Zürich, Zürich, SWITZERLAND

Address for correspondence: Carsten Lundby, Ph.D., Institute of Physiology and Zürich Center for Integrative Human Physiology, University of Zürich, Office 23 H 6, Winterthurerstrasse 190, 8057 Zürich, Switzerland; E-mail:

Submitted for publication November 2013.

Accepted for publication February 2014.

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© 2014 American College of Sports Medicine