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00005768-201010002-0004700005768_2010_42_20_yfanti_supplementation_10abstract< 11_0_1_0 >Medicine & Science in Sports & Exercise©2010The American College of Sports MedicineVolume 42(10) Supplement 2October 2010p 20Role of Antioxidant Supplementation on Training-induced IL-6[THURSDAY, SEPTEMBER 23, 2010, 1:00-3:00PM POSTER SESSION 1: Board #47: Exercise, Oxidative Stress, and Redox Signaling]Yfanti, Christina1; Fischer, Christian P.1; Nielsen, Søren1; Åkerström, Thorbjörn1; Nielsen, Anders R.1; Veskoukis, Aristidis S.2; Kouretas, Demetrios2; Lykkesfeldt, Jens3; Pilegaard, Henriette4; Pedersen, Bente K.11Centre of Inflammation and Metabolism, Copenhagen, Denmark. 2University of Thessaly, Larisa, Greece. 3Section of Biomedicine, University of Copenhagen, Copenhagen, Denmark. 4August Krogh Institute, University of Copenhagen, Copenhagen, Denmark.Email: christinayfanti@inflammation-metabolism.dkAntioxidant supplementation has been shown to attenuate the acute exercise-induced increase in plasma interleukin-6 (IL-6). PURPOSE: To investigate the effect of antioxidants on the regulation of IL-6 expression in muscle and circulation in response to acute exercise before and after high-intensity endurance exercise training. METHODS: Twenty-one young healthy men were allocated into either an antioxidant (AO; vitamin C and E) or a placebo (PL) group. A 1h-acute bicycling exercise trial at 65% of maximal power output was performed before and after 12 weeks of progressive endurance exercise training. Blood samples and muscle biopsies were taken pre and immediately after cessation of exercise, and at 3h of recovery period. RESULTS: The acute exercise-induced plasma IL-6 increase was blunted after the training period in the PL group (P<0.05) but not in the AO group. Endurance exercise training lowered resting levels and attenuated the acute exercise-induced increase in muscle-IL-6 mRNA in both groups. There was an overall increase in skeletal muscle oxidative stress as evaluated by malondialdehyde (MDA) with training in the AO group compared with PL (P<0.05). This was accompanied by a general increase in skeletal muscle mRNA expression of antioxidative enzymes, including catalase (CAT), copper-zinc superoxide dismutase (CuZnSOD) and glutathione peroxidase 1 (GPX1) mRNA expression in the AO group. However, skeletal muscle protein content of catalase, CuZnSOD, or GPX1 was not affected by training or supplementation. CONCLUSIONS: Vitamin C and E supplementation blunts the training-induced lowering of exercise-induced increase in plasma IL-6, possibly through increased oxidative stress in the trained state.Role of Antioxidant Supplementation on Training-induced IL-6Yfanti, Christina; Fischer, Christian P.; Nielsen, Søren; Åkerström, Thorbjörn; Nielsen, Anders R.; Veskoukis, Aristidis S.; Kouretas, Demetrios; Lykkesfeldt, Jens; Pilegaard, Henriette; Pedersen, Bente K.THURSDAY, SEPTEMBER 23, 2010, 1:00-3:00PM POSTER SESSION 1: Board #47: Exercise, Oxidative Stress, and Redox Signaling1042