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00005768-200905001-0217400005768_2009_41_191_klau_accumulation_5miscellaneous< 17_0_1_0 >Medicine & Science in Sports & Exercise©2009The American College of Sports MedicineVolume 41(5) Supplement 1May 2009p 191Rate Of D2O Accumulation With Rehydration Using Three Different Drinks: 1974: Board #115 May 28 9:00 AM - 10:30 AM[C-33 Free Communication/Poster - Hydration Status and Rehydration: MAY 28, 2009 7:30 AM - 12:30 PM ROOM: Hall 4F]Klau, Jennifer F.; Ganio, Matthew S.; Lee, Elaine C.; Yamamoto, Linda M.; McDermott, Brendon P.; Lopez, Rebecca M.; Anderson, Jeffrey M.; Volek, Jeff S.; Armstrong, Lawrence E. FACSM; Kraemer, William J. FACSM; Maresh, Carl M. FACSMUniversity of Connecticut, Storrs, CT.Email: jennifer.klau@uconn.edu(No relationships reported)The availability of fluid consumed for thermoregulation and hydration depends on the rate of gastric emptying and fluid absorption in the intestine. Deuterium Oxide (D2O) accumulation serves as a marker of fluid uptake and allows for the assessment of the relative temporal availability of a fluid after ingestion.PURPOSE: To compare the rate of D2O accumulation during rehydration with three different beverages at rest.METHODS: Twelve males (mean ± SD age, 25 ± 3y; mass, 75.91 ± 5.94kg) completed three dehydration trials in a randomized, blocked experimental design. Subjects walked for 1 hour (3.9 ± 0.3mph; 1.5 ± 0.6% incline) in 30 ± 1°C, rh 50 ± 7% followed by a 24h fluid restriction resulting in dehydration of -2.69 ± 1.11. Subjects then ingested D2O (0.15 g/kg body weight) in either distilled water (WA), a carbohydrate - electrolyte (CE) beverage, or a flavored vitamin drink (VF) in four equal boluses consumed during the initial 5 minutes of a 60 minute rehydration/seated rest period. The total volume of fluid consumed was 50% of body weight lost. Plasma D2O and serum osmolality (Osm) were measured at 0, 4, 8, 12, 16, 20, 45 and 60 min. Thirst was assessed at 0, 4, 12, 20 and 60 min. Two-way and one-way repeated-measures ANOVA were conducted with alpha set at 0.05. Post hoc analyses were adjusted using a Bonferroni correction.RESULTS: There was no interaction (p>0.05) between beverage and D2O accumulation or beverage and attenuation of thirst. Mean D2O accumulation in plasma increased (p<0.05) at every time point after ingestion except between minutes eight and 12 and between minutes 45 and 60. D2O accumulation increased 51.85 ±11.64% four minutes after ingestion and 78.21 ± 2.98% during the 60 minutes. Serum Osm declined (p<0.05) from min 0 to min 60 min in VF and WA, but not in CE. Mean thirst was 7 ±1 pre-rehydration and 3 ±2 at 60 min. Decrease in thirst was greater (p<0.05) for VF than CE, but not for VF compared to WA or WA compared to CE.CONCLUSION: Using D2O accumulation as a marker of fluid uptake, rehydration resulted in a similar rate of uptake among trials. Drink VF attenuated thirst more than CE and WA.Supported by glacéau (a division of The Coca-Cola Company).Rate Of D2O Accumulation With Rehydration Using Three Different Drinks: 1974: Board #115 May 28 9:00 AM - 10:30 AMKlau, Jennifer F.; Ganio, Matthew S.; Lee, Elaine C.; Yamamoto, Linda M.; McDermott, Brendon P.; Lopez, Rebecca M.; Anderson, Jeffrey M.; Volek, Jeff S.; Armstrong, Lawrence E. FACSM; Kraemer, William J. FACSM; Maresh, Carl M. FACSMC-33 Free Communication/Poster - Hydration Status and Rehydration: MAY 28, 2009 7:30 AM - 12:30 PM ROOM: Hall 4F541