Inhaled Beta2-Agonist Increases Power Output and Glycolysis during Sprinting in Men

KALSEN, ANDERS1,2; HOSTRUP, MORTEN1,2; SÖDERLUND, KARIN3; KARLSSON, SEBASTIAN1; BACKER, VIBEKE1; BANGSBO, JENS2

Medicine & Science in Sports & Exercise: January 2016 - Volume 48 - Issue 1 - p 39–48
doi: 10.1249/MSS.0000000000000732
Basic Sciences

Purpose: The aim of the present study was to investigate the effects of the beta2-agonist terbutaline (TER) on power output and muscle metabolism during maximal sprint cycling.

Methods: In a randomized double-blind cross-over design, nine moderately trained men (V˙O2max = 4.6 ± 0.2 L·min−1) conducted a 10-s cycle sprint after inhalation of either 15 mg of TER or placebo (PLA). A muscle biopsy sample was collected before and <10 s after the sprint and was analyzed for metabolites.

Results: The mean power and peak power during the sprint were 8.3% ± 1.1% and 7.8% ± 2.5% higher (P < 0.05) with TER than with PLA, respectively. Moreover, the net rates of glycogenolysis (6.5 ± 0.8 vs 3.1 ± 0.7 mmol glucosyl units·kg dry weight−1·s−1) and glycolysis (2.4 ± 0.2 vs 1.6 ± 0.2 mmol glucosyl units·kg dry weight−1·s−1) were higher (P < 0.05) with TER than with PLA. After the sprint, adenosine triphosphate (ATP) was reduced with PLA (P < 0.05) but not with TER. During the sprint, there was no difference in the breakdown of phosphocreatine (PCr) between treatments. Estimated anaerobic ATP utilization was 9.2% ± 4.0% higher (P < 0.05) with TER than with PLA. After the sprint, ATP in Type II fibers was lowered (P < 0.05) by 25.7% ± 7.3% with PLA but was not reduced with TER. Before the sprint, PCr in Type II fibers was 24.5% ± 7.2% lower (P < 0.05) with TER than with PLA. With PLA, breakdown of PCr was 50.2% ± 24.8% higher (P < 0.05) in Type II fibers (vs Type I fibers), whereas no difference was observed between fiber types with TER.

Conclusion: The present study shows that a TER-induced increase in power output is associated with increased rates of glycogenolysis and glycolysis in skeletal muscles. Furthermore, as TER counteracts a reduction in ATP in Type II fibers, TER may postpone fatigue development in these fibers.

1Respiratory Research Unit, Bispebjerg University Hospital, Copenhagen, DENMARK; 2Section of Integrated Physiology, Department of Nutrition, Exercise, and Sports, University of Copenhagen, Copenhagen, DENMARK; and 3The Swedish School of Sport and Health Sciences, Stockholm, SWEDEN

Address for correspondence: Jens Bangsbo, Professor, Dr. Sci, Universitetsparken 13, Copenhagen 2100, Denmark; E-mail: jbangsbo@nexs.ku.dk.

Submitted for publication March 2015.

Accepted for publication July 2015.

© 2016 American College of Sports Medicine