Impact of Various Exercise Modalities on Hepatic Mitochondrial Function

FLETCHER, JUSTIN A.1,2; MEERS, GRACE M.1,3; LINDEN, MELISSA A.1,2; KEARNEY, MONICA L.1,2; MORRIS, E. MATTHEW1,3; THYFAULT, JOHN P.1,2,3; RECTOR, R. SCOTT1,2,3

Medicine & Science in Sports & Exercise: June 2014 - Volume 46 - Issue 6 - p 1089–1097
doi: 10.1249/MSS.0000000000000223
Basic Sciences

Purpose: Hepatic mitochondrial adaptations to exercise are largely unknown. In this study, we sought to determine the effects of various exercise modalities on measures of hepatic mitochondrial function and metabolism.

Methods: Male Sprague–Dawley rats were randomly assigned (n = 8–10 per group) into sedentary (SED), voluntary wheel running (VWR), VWR with food pulled during the dark cycle (VMR-OF), treadmill endurance exercise (TM-END; 30 m·min−1, 12% gradient, 60 min·d−1, 5 d·wk−1), or treadmill interval sprint training (TM-IST; 50 m·min−1, 12% gradient, 6 × 2.5 min bouts, 5 d·wk−1) groups for a 4-wk intervention.

Results: Hepatic mitochondrial state 3 and maximal uncoupled respiration were significantly (P < 0.05) increased in all four exercise groups compared with SED animals. In addition, hepatic mitochondrial [1-14C] pyruvate oxidation to CO2, an index of pyruvate dehydrogenase (PDH) activity, was significantly increased in VWR-OF, TM-END, and TM-IST rats (P < 0.05), whereas exercise-induced increases in [2-14C] pyruvate oxidation and [1-14C] palmitate oxidation to CO2 did not reach statistical significance. Hepatic mitochondrial sirtuin 3 protein content, which putatively increases activity of mitochondrial proteins, was elevated in the VWR, VWR-OF, and TM-END groups (P < 0.05). In addition, only VWR-OF animals experienced increases in hepatic cytochrome c protein content and phosphoenolpyruvate carboxykinase mRNA, whereas PGC-1α mRNA expression and phospho-CREB protein content was increased in VWR-OF and TM-END groups.

Conclusion: Four weeks of exercise training, regardless of exercise modality, significantly increased hepatic mitochondrial respiration and evoked other unique improvements in mitochondrial metabolism that do not appear to be dependent on increases in mitochondrial content.

1Harry S. Truman Memorial Veterans’ Hospital, Columbia, MO; 2Department of Nutrition and Exercise Physiology, University of Missouri, Columbia, MO; and 3Division of Gastroenterology and Hepatology, Department of Internal Medicine, University of Missouri, Columbia, MO

Address for correspondence: R. Scott Rector, Ph.D., Harry S. Truman Memorial Veterans’ Hospital; Division of Gastroenterology and Hepatology, Department of Internal Medicine; and Department of Nutrition and Exercise Physiology, University of Missouri–Columbia, Columbia, MO 65212; E-mail: rectors@health.missouri.edu.

Submitted for publication July 2013.

Accepted for publication November 2013.

© 2014 American College of Sports Medicine