CMV Amplifies T-cell Redeployment to Acute Exercise Independently of HSV-1 Serostatus

LAVOY, EMILY C.; BIGLEY, AUSTIN B.; SPIELMANN, GUILLAUME; RECTOR, JERRALD L.; MORRISON, MARK R.; O’CONNOR, DANIEL P.; SIMPSON, RICHARD J.

Medicine & Science in Sports & Exercise: February 2014 - Volume 46 - Issue 2 - p 257–267
doi: 10.1249/MSS.0b013e3182a5a0fb
Basic Sciences

Purpose: Latent cytomegalovirus (CMV) infection has been shown to alter the lymphocyte response to acute aerobic exercise, likely due to the corresponding increase in exercise-responsive memory CD8+ T cells. It is unknown if latent infection with another herpesvirus, herpes simplex virus 1 (HSV-1), also plays a role in shaping the lymphocyte response to exercise.

Methods: Thirty-two men (ages 39.3 ± 14.7 yr) counterbalanced by CMV and HSV-1 serostatus (positive/negative) cycled for 30 min at ∼80% peak power. Blood sampled before, immediately after, and 1 h after exercise was analyzed by flow cytometry for T-cell subset enumeration.

Results: In resting blood, HSV-1+ had fewer lymphocytes, CD4+ T cells, KLRG1CD28+CD4+ T cells, and CD45RACCR7+CD4+ T cells than HSV-1, whereas CMV+ had increased numbers of lymphocytes, CD8+ T cells, KLRG1+CD28CD4+ and CD8+ T cells, and CD45RA+CCR7CD8+ T cells and a lower CD4:CD8 T-cell ratio than CMV. After exercise, CMV+ had a greater mobilization of CD8+ T cells, KLRG1+CD28CD4+ and CD8+ T cells, and CD45RA+CCR7CD8+ T cells independently of HSV-1 serostatus, as well as a greater egress of these subsets 1 h after exercise. HSV serostatus did not influence total CD8+ T-cell response to exercise.

Conclusions: The impact of latent CMV infection on the redeployment of T-cell subsets with exercise is independent of HSV-1 infection. This is most likely due to the unique ability of CMV to alter the composition of the memory T-cell pool in favor of exercise-responsive T-cell subsets.

Laboratory of Integrated Physiology, Department of Health and Human Performance, University of Houston, Houston, TX

Address for correspondence: Emily C. LaVoy, Laboratory of Integrated Physiology, Department of Health and Human Performance, University of Houston, 3855 Holman St, Houston, TX 77204; E-mail: eclavoy@uh.edu.

Submitted for publication April 2013.

Accepted for publication July 2013.

© 2014 American College of Sports Medicine