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Biphasic Stress Response in the Soleus during Reloading after Hind Limb Unloading

LAWLER, JOHN M.1; KWAK, HYO-BUM1; KIM, JONG-HEE1; LEE, YANG1; HORD, JEFFREY M.1; MARTINEZ, DANIEL A.2

Medicine & Science in Sports & Exercise: April 2012 - Volume 44 - Issue 4 - p 600–609
doi: 10.1249/MSS.0b013e31823ab37a
Basic Sciences

Introduction: Extreme disuse and spaceflight elicit rapid skeletal muscle atrophy, accompanied by elevated proinflammatory signaling and impaired stress response proteins (e.g., heat shock proteins (HSP), insulin-like growth factor 1 (IGF-1)). Recovery of muscle mass is delayed during the early stage of reloading after prolonged unloading, with a concomitant impairment of HSP70 and IGF-1. We postulated that proinflammatory signaling and stress response alterations would characterize early and late phases of signaling during reloading.

Methods: Twenty-four adult SD rats were divided into the following groups: controls, 28 d of hind limb unloading (HU), HU + early (7 d) reloading (HU-R7), and HU + late (28 d) reloading (HU-R28).

Results: Soleus mass decreased (−55%) with HU and remained depressed (−41%) at HU-R7. Nuclear factor κB activation and oxidative stress were elevated with HU and remained high during reloading. HU elevated inducible nitric oxide synthase and returned to baseline during reloading, whereas 3-nitrotyrosine did not increase with HU and peaked at HU-R7. HU depressed levels of HSP25 phosphorylation at Ser82 and IGF-1. Although p-HSP25 and Akt phosphorylation (Ser473) recovered during early reloading, HSP70, heat shock factor 1, and IGF-1 remained depressed. HSP70, heat shock factor 1, and IGF-1 recovered, whereas p-Akt and 3-nitrotyrosine decreased to control levels at HU-R28.

Conclusions: Reloading elicited an early phase characterized by elevated nuclear factor κB activation, 3-nitrotyrosine, p-HSP25, and p-Akt levels and a delayed phase with recovery of HSP70, IGF-1, and muscle mass. We conclude that the reloading phenotype in skeletal muscle is expressed in two distinct phases related to (a) pro-inflammatory signaling and (b) muscle mass recovery.

1Texas A&M University, Redox Biology and Cell Signaling Laboratory, College Station, TX; and 2Department of Mechanical Engineering, University of Houston, Houston, TX

Address for correspondence: John M. Lawler, Ph.D., Redox Biology & Cell Signaling Laboratory, Department of Health and Kinesiology, Texas A&M University, College Station, TX 77843-4243; E-mail: jml2621@neo.tamu.edu.

Submitted for publication March 2011.

Accepted for publication September 2011.

©2012The American College of Sports Medicine