Skip Navigation LinksHome > December 2011 - Volume 43 - Issue 12 > Human Muscle Proteome Modifications after Acute or Repeated...
Medicine & Science in Sports & Exercise:
doi: 10.1249/MSS.0b013e318222edf3
Basic Sciences

Human Muscle Proteome Modifications after Acute or Repeated Eccentric Exercises

HODY, STÉPHANIE1; LEPRINCE, PIERRE1; SERGEANT, KJELL2; RENAUT, JENNY2; CROISIER, JEAN-LOUIS3,4; WANG, FRANÇOIS4; ROGISTER, BERNARD1,5,6

Supplemental Author Material
Collapse Box

Abstract

Introduction: Delayed-onset muscle soreness (DOMS), a condition triggered by eccentric exercise, affects muscle cells at a biochemical level in a poorly understood fashion. The objective of the present study was to examine human muscle proteome modifications induced by strenuous eccentric exercises after a specific training aimed to prevent DOMS.

Methods: Biopsy samples of the rectus femoris were obtained from healthy human volunteers in three successive conditions: 1) at rest, 2) 24 h after an injuring exercise protocol consisting of three series of 30 maximal contractions of the quadriceps on an isokinetic dynamometer, and 3) 24 h after a similar exercise bout preceded either by five eccentric training sessions or by no training.

Results: Muscle damage was assessed before and 1 d after each maximal eccentric test by comparing three indirect markers: plasma activity of creatine kinase, muscle stiffness, and subjective pain intensity. Compared with the first eccentric test, those markers were reduced after the second test and further reduced if this second test followed the eccentric training, thus confirming the protective effect of such training. Muscle protein extracts were subjected to a two-dimensional difference gel electrophoresis proteomic analysis coupled with matrix-assisted laser desorption/ionization–time-of-flight mass spectrometry protein identification. Surprisingly, we observed that myosin heavy chains decreased after the first eccentric test and were reduced further with other contractile proteins after the second test. Furthermore, the expression of several glycolytic enzymes decreased only after the second test, which was preceded by a specific training.

Conclusions: These findings suggest that the eccentric training resulted in a switch to oxidative metabolism, which may be associated with protection from DOMS.

©2011The American College of Sports Medicine

Login

Article Tools

Share

Search for Similar Articles
You may search for similar articles that contain these same keywords or you may modify the keyword list to augment your search.

Connect With Us