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Amylin and Selective Glucoregulatory Peptide Alterations during Prolonged Exercise


Medicine & Science in Sports & Exercise: August 2011 - Volume 43 - Issue 8 - p 1451-1456
doi: 10.1249/MSS.0b013e3182114ab9
Basic Sciences

ABSTRACT Amylin is a pancreatic β-cell peptide that facilitates the regulation of blood glucose concentration by inhibiting release of glucagon and modulating gastric emptying. Prolonged exercise may alter amylin and aid in the maintenance of blood glucose concentration; however, no studies have investigated the effects of prolonged exercise on amylin.

Purpose: This study aimed to determine the effects of 90 min of treadmill exercise on amylin and other glucoregulatory hormone responses in a postprandial state.

Methods: Eight young healthy males completed a preliminary trial for V˙O2max and body composition determination and subsequent experimental and control trials in a counterbalanced manner. The experimental trial subjects arrived at the laboratory at 8:00 a.m., 1 h after consumption of a standard nutrient beverage (Ensure Plus®). At 9:50 a.m., subjects initiated 90 min of treadmill exercise at 60% of V˙O2max. Blood samples were collected twice before exercise, every 18 min during exercise, and every 20 min during 1 h of recovery. A resting control trial was conducted in an identical manner without V˙O2 assessment.

Results: Plasma glucose and leptin concentrations remained stable across exercise, whereas lactate significantly increased to peak at 18 min of exercise then gradually declined. Amylin, insulin, and C-peptide values significantly declined over the trials, with no difference between exercise and control days. Glucagon area-under-the-curve concentrations were significantly greater during the exercise than the control trials. There was a significant time effect and trial effect for cortisol with a higher concentration during the experimental trial than during the control trial.

Conclusions: In a postprandial state, prolonged exercise stimulates glucagon and cortisol increases that are associated with stable blood glucose and leptin concentrations; however, similar to postprandial state control condition, insulin, C-peptide, and amylin concentrations decline.

1Department of Kinesiology and Health Studies, Southeastern Louisiana University, Hammond, LA; 2Department of Biological Sciences, Southeastern Louisiana University, Hammond, LA; and 3Department of Obstetrics and Gynecology, Texas Tech University Health Sciences Center, Odessa, TX

Address for correspondence: Robert R. Kraemer, FACSM, Southeastern Louisiana University, Department of Kinesiology and Health Studies, SLU10845, Hammond, LA; E-mail:

Submitted for publication September 2010.

Accepted for publication January 2011.

©2011The American College of Sports Medicine