Skip Navigation LinksHome > July 2011 - Volume 43 - Issue 7 > Cell-Derived Microparticles Promote Coagulation after Modera...
Medicine & Science in Sports & Exercise:
doi: 10.1249/MSS.0b013e3182068645
Basic Sciences

Cell-Derived Microparticles Promote Coagulation after Moderate Exercise

SOSSDORF, MAIK1; OTTO, GORDON P.1; CLAUS, RALF A.1; GABRIEL, HOLGER H. W.2; LÖSCHE, WOLFGANG1

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Abstract

Cell-derived procoagulant microparticles (MP) might be able to contribute to exercise-induced changes in blood hemostasis.

Purposes: This study aimed to examine (i) the concentration and procoagulant activity of cell-derived MP after a moderate endurance exercise and (ii) the differences in the release, clearance, and activity of MP before and after exercise between trained and untrained individuals.

Methods: All subjects performed a single bout of physical exercise on a bicycle ergometer for 90 min at 80% of their individual anaerobic threshold. MP were identified and quantified by flow cytometry measurements. Procoagulant activity of MP was measured by a prothrombinase activity assay as well as tissue factor-induced fibrin formation in MP-containing plasma.

Results: At baseline, no differences were observed for the absolute number and procoagulant activities of MP between trained and untrained subjects. However, trained individuals had a lower number of tissue factor-positive monocyte-derived MP compared with untrained individuals. In trained subjects, exercise induced a significant increase in the number of MP derived from platelets, monocytes, and endothelial cells, with maximum values at 45 min after exercise and returned to basal levels at 2 h after exercise. Untrained subjects revealed a similar increase in platelet-derived MP, but their level was still increased at 2 h after exercise, indicating a reduced clearance compared with trained individuals. Procoagulant activities of MP were increased immediately after exercise and remained elevated up to 2 h after exercise.

Conclusions: We conclude that increased levels of MP were found in healthy individuals after an acute bout of exercise, that the amount of circulating MP contributes to an exercise-induced increase of hemostatic potential, and that there were differences in kinetic and dynamic characteristics between trained and untrained individuals.

©2011The American College of Sports Medicine

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