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Muscle Deoxygenation during Sustained and Intermittent Isometric Exercise in Hypoxia

KATAYAMA, KEISHO1,2; YOSHITAKE, YASUHIDE3; WATANABE, KOHEI2; AKIMA, HIROSHI1,2; ISHIDA, KOJI1,2

Medicine & Science in Sports & Exercise: July 2010 - Volume 42 - Issue 7 - pp 1269-1278
doi: 10.1249/MSS.0b013e3181cae12f
BASIC SCIENCES: Contrasting Perspectives

Purpose: It is reported that the rate of locomotor muscle fatigue development during intermittent isometric exercise in hypoxia is accelerated compared with normoxia. In contrast, when sustained isometric contractions are used, some studies do not show any effect of hypoxia on fatigue development. Increased intramuscular pressure during sustained isometric exercise causes substantial and sustained ischemia, even in normoxia. Therefore, we hypothesized that the difference in muscle deoxygenation between normoxia and hypoxia would be small during sustained exercise compared with intermittent exercise and that this may contribute to the inconsistent findings.

Methods: Subjects performed sustained and intermittent isometric, unilateral, and submaximal knee-extension exercises (60% maximal voluntary contraction to exhaustion) while breathing normoxic (inspired O2 fraction = 0.21) or hypoxic gas mixtures (inspired O2 fraction = 0.10-0.12). Muscle oxygenation (deoxyhemoglobin/myoglobin and tissue oxygenation index) using near-infrared spectroscopy and surface EMG were measured from the left vastus lateralis.

Results: During intermittent isometric exercise in hypoxia, increases in deoxyhemoglobin/myoglobin and reductions of tissue oxygenation index were larger (P < 0.05) than those in normoxia. The rate of rise in integrated EMG during intermittent exercise was accelerated (P < 0.05) in hypoxia. In contrast, there were no significant differences in changes in near-infrared spectroscopy variables and integrated EMG during sustained isometric exercise between normoxia and hypoxia.

Conclusions: These results suggest that muscle deoxygenation is exaggerated during intermittent isometric exercise in hypoxia compared with normoxia, whereas during sustained isometric exercise, the extent of muscle deoxygenation is the same between normoxia and hypoxia. The different extent of muscle deoxygenation during sustained and intermittent isometric exercise in normoxia and hypoxia could affect muscle fatigability, which results from the varied rate of accumulation of metabolites.

1Research Center of Health, Physical Fitness and Sports, Nagoya University, JAPAN, 2Graduate School of Education and Human Development, Nagoya University, JAPAN; and 3Department of Physiological Sciences, National Institute of Fitness and Sports, JAPAN

Address for correspondence: Keisho Katayama, Ph.D., Research Center of Health, Physical Fitness and Sports, Nagoya University, Furocho, Chikusaku, Nagoya, 464-8601, Japan; E-mail: katayama@htc.nagoya-u.ac.jp.

Submitted for publication June 2009.

Accepted for publication November 2009.

©2010The American College of Sports Medicine