Skip Navigation LinksHome > April 2010 - Volume 42 - Issue 4 > Model for Muscle Regeneration around Fibrotic Lesions in Rec...
Medicine & Science in Sports & Exercise:
doi: 10.1249/MSS.0b013e3181beeb52
SPECIAL COMMUNICATIONS: Methodological Advances

Model for Muscle Regeneration around Fibrotic Lesions in Recurrent Strain Injuries

GREFTE, SANDER1; KUIJPERS-JAGTMAN, ANNE MARIE1; TORENSMA, RUURD2; VON DEN HOFF, JOHANNES W.1

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Abstract

Purpose: The purpose of this study was to establish an in vivo model for muscle regeneration after strain injury in the presence of a fibrotic discontinuity.

Methods: The musculus soleus of 5-wk-old male rats was exposed, completely lacerated, and sutured together with or without a collagen scaffold in between the muscle ends. The scaffold represents a fibrotic discontinuity in the muscle. Muscle healing was evaluated after 14 d by general histology and staining for myofibroblasts, satellite cells (activated), and inflammatory cells.

Results: Around all wounds, satellite cells were activated. Inside the collagen scaffolds, satellite cells were absent, indicating that muscle regeneration was impaired. In the wounds without a collagen scaffold, the lacerated and the sutured myofibers contacted and had already started to regenerate, whereas this did not occur with an implanted scaffold.

Conclusions: A fibrotic discontinuity, such as an implanted collagen scaffold, delays muscle regeneration in skeletal muscle. This model is suitable to study skeletal muscle regeneration in the presence of a fibrotic lesion and to evaluate new treatment modalities for muscle strain injuries.

©2010The American College of Sports Medicine

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