Influence of Delivery Mode on the Urinary Excretion of Nandrolone Metabolites

WATSON, PHILLIP1; HOUGHTON, ED2; GRACE, PHILIP B.2; JUDKINS, CATHERINE2; DUNSTER, PAULA M.2; MAUGHAN, RONALD J.1

Medicine & Science in Sports & Exercise:
doi: 10.1249/MSS.0b013e3181bf94d6
Basic Sciences
Abstract

Introduction: This study examined the influence of a supplement matrix on the excretion pattern of nandrolone metabolites in response to ingestion of a trace amount of 19-norandrostenedione.

Methods: Ten male and nine female volunteers (mean ± SD: age = 26 ± 3 yr, height = 1.71 ± 0.09 m, body mass = 70.9 ± 13.2 kg) were recruited. On two occasions, subjects entered the laboratory in the morning after an overnight fast. After an initial urine collection, subjects ingested either 500 mL of plain water or a commercially available energy bar; 10 μg of 19-norandrostenedione was added to each. The volume of each urine sample passed during the next 24 h was measured, and an aliquot was retained for analysis. All samples were analyzed for the metabolites 19-norandrosterone (19-NA) and 19-noretiocholanolone (19-NE) by gas chromatography-mass spectrometry.

Results: The total volume of urine passed was higher in the water trial (2.10 ± 0.52 L) than in the bar trial (1.85 ± 0.55 L; P = 0.040). Baseline urinary 19-NA concentrations were all below the limit of quantification for the assay. Peak urinary 19-NA was lower (P = 0.002) in the water trial (4.80 ± 2.84 ng·mL−1) than in the bar trial (8.46 ± 4.44 ng·mL−1). The time elapsed between ingestion of the supplement and the peak urinary 19-NA concentration was longer (P = 0.023) on the bar trial (4.6 ± 2.4 h) than on the water trial (2.8 ± 1.9 h). There was no difference in the total recovery of 19-NA + 19-NE between the liquid and solid supplements (water 30 ± 10%; bar 28 ± 12%; P < 0.140).

Conclusions: Peak 19-NA concentrations were higher, and occurred later, when the 19-norandrostenedione was added to a solid supplement. This may be due to a slower rate of absorption and/or a reduced diuresis, resulting in a longer period for the metabolites to accumulate in the urine.

Author Information

1School of Sport and Exercise Sciences, Loughborough University, Loughborough, UNITED KINGDOM; and 2HFL Sport Science (Quotient Bioresearch Ltd.), Fordham, Cambridgeshire, UNITED KINGDOM

Address for correspondence: Ronald J. Maughan, Ph.D., School of Sport and Exercise Sciences, Loughborough University, Leicestershire, LE11 3TU, United Kingdom; E-mail: r.j.maughan@lboro.ac.uk.

Submitted for publication June 2009.

Accepted for publication September 2009.

©2010The American College of Sports Medicine