Protein Modification Responds to Exercise Intensity and Antioxidant Supplementation

LAMPRECHT, MANFRED1,2; OETTL, KARL1; SCHWABERGER, GUENTHER3; HOFMANN, PETER4,5,; GREILBERGER, JOACHIM F.1

Medicine & Science in Sports & Exercise:
doi: 10.1249/MSS.0b013e31818338b7
Basic Sciences
Abstract

Purpose: To assess the effects of different exercise intensities and antioxidant supplementation on plasma protein modification.

Methods: Trained men (n = 41) from a homogenous population were randomly assigned to perform cycle ergometer exercise either at 70% or 80% of individual V˙O2max. Each intensity group was randomly assigned to receive either juice powder concentrate (JPC 70%, n = 11; JPC 80%, n = 10) or placebo (Plac 70%, n = 10; Plac 80%, n = 10) capsules for 28 wk. Four controlled exercise bouts and blood collections were conducted at baseline and study weeks 4, 16, and 28. Blood samples were drawn before (BE), immediately after (IE), and 30 min (30M) and 30 h (30H) postexercise. These samples were analyzed to estimate concentrations of carbonyl groups on plasma proteins (CP) and the redox state of human serum albumin (HSA).

Results: In the Plac group, CP concentrations increased at 80% of V˙O2max IE and 30M, returning to preexercise concentrations by 30H (P < 0.05). At both 16 and 28 wk, the Plac groups had significantly higher BE and 30H CP concentrations than the JPC groups (P < 0.05). The reduced fraction of HSA, human mercaptalbumin (HMA), decreased at all four exercise tests at both exercise intensities IE and 30M, returning to preexercise values by 30H (P < 0.05). Supplementation had no influence on HSA.

Conclusions: These results indicate that CP concentrations increase with 80% V˙O2max intensity. The JPC group had lower baseline CP levels after 16 and 28 wk and no exercise-induced CP increase. HSA is reversibly shifted to a more oxidized state by recent intense exercise.

Author Information

1Institute of Physiological Chemistry, Medical University of Graz, Graz, AUSTRIA; 2Styrian Health Association, Graz, AUSTRIA; 3Institute of Physiology, Medical University of Graz, Graz, AUSTRIA; 4Institute of Sports Science, Karl Franzens University of Graz, Graz, AUSTRIA; and 5Human Performance Research Graz, Karl Franzens University and Medical University of Graz, Graz, AUSTRIA

Address for correspondence: Manfred Lamprecht, Ph.D., Institute of Physiological Chemistry, Harrachgasse 21/II, Medical University of Graz, 8010 Graz, Austria; E-mail: manfred.lamprecht@meduni-graz.at.

Submitted for publication April 2008.

Accepted for publication June 2008.

© 2009 American College of Sports Medicine