Skip Navigation LinksHome > April 2008 - Volume 40 - Issue 4 > Human Muscle Gene Expression following Resistance Exercise a...
Medicine & Science in Sports & Exercise:
doi: 10.1249/MSS.0b013e318160ff84
BASIC SCIENCES: Original Investigations

Human Muscle Gene Expression following Resistance Exercise and Blood Flow Restriction

DRUMMOND, MICAH J.1,3; FUJITA, SATOSHI1,4; TAKASHI, ABE4; DREYER, HANS C.1,3; VOLPI, ELENA1; RASMUSSEN, BLAKE B.1,3

Collapse Box

Abstract

Introduction: Blood flow restriction in combination with low-intensity resistance exercise (REFR) increases skeletal muscle size to a similar extent as compared with traditional high-intensity resistance exercise training. However, there are limited data describing the molecular adaptations that occur after REFR.

Purpose: To determine whether hypoxia inducible factor-1 alpha (HIF-1α) and REDD1 mRNA are expressed differently in REFR compared with low-intensity resistance exercise with no blood flow restriction (CONTROL). Secondly, to determine whether low-intensity resistance exercise is able to induce changes in mRNA expression of several anabolic and catabolic genes as typically seen with high-intensity resistance exercise.

Methods: Six subjects were studied at baseline and 3 h after a bout of leg resistance exercise (20% 1RM) in REFR and CONTROL subjects. Each subject participated in both groups, with 3 wk separating each visit. Muscle biopsy samples were analyzed for mRNA expression, using qRT-PCR.

Results: Our primary finding was that there were no differences between CONTROL and REFR for any of the selected genes at 3 h after exercise (P > 0.05). However, low-intensity resistance exercise increased HIF-1α, p21, MyoD, and muscle RING finger 1 (MuRF1) mRNA expression and decreased REDD1 and myostatin mRNA expression in both groups (P < 0.05).

Conclusion: Low-intensity resistance exercise can alter skeletal muscle mRNA expression of several genes associated with muscle growth and remodeling, such as REDD1, HIF-1α, MyoD, MuRF1, and myostatin. Further, the results from REFR and CONTROL were similar, indicating that the changes in early postexercise gene expression were attributable to the low-intensity resistance exercise bout, and not blood flow restriction.

©2008The American College of Sports Medicine

Login

Article Tools

Share

Search for Similar Articles
You may search for similar articles that contain these same keywords or you may modify the keyword list to augment your search.

Connect With Us