Effects of Unaccustomed and Accustomed Exercise on the Immune Response in Runners

SORICHTER, STEPHAN1; MARTIN, MARIA1; JULIUS, PETER2; SCHWIRTZ, ANSGAR3; HUONKER, MARTIN4; LUTTMANN, WERNER2; WALTERSPACHER, STEPHAN1; BERG, ALOYS4

Medicine & Science in Sports & Exercise: October 2006 - Volume 38 - Issue 10 - pp 1739-1745
doi: 10.1249/01.mss.0000230213.62743.fb
BASIC SCIENCES: Original Investigations

Purpose: To test the hypothesis that long-term immunological response may be different after accustomed concentric and unaccustomed eccentric exercise in endurance-trained men.

Methods: Fourteen highly endurance-trained male runners performed two bouts of high-intensity exercise with at least 2-wk intervals between bouts. Concentric exercise consisted of a 60-min level run with a targeted heart rate of 80% V˙O2peak. Eccentric exercise was conducted lying on a specially designed exercise rack, eliciting eccentric action of the musculus quadriceps femoris. Blood samples were taken before and 1, 6, 24, 72, and 144 h after exercise to determine creatine kinase (CK), C-reactive protein (CRP), and interleukin-6 (IL-6). Lymphocyte subset distribution was assessed using flow cytometry.

Results: We found a significant (P < 0.05) increase of CD4+ (eccentric: 17%; concentric: 20%), CD3+/CD4+ (16 vs 19%), CD25+ (45 vs 29%), CD25+/CD4+ (27 vs 50%), HLA-DR+ (20 vs 15%), HLA-DR+/CD4+ (16 vs 67%), and CD19+/CD45+ (52 vs 103%) positive lymphocytes 1 h after both exercise bouts. However, eccentric exercise resulted in a significantly higher and longer (6 h) increase of CD25+/CD4+ and HLA-DR+/CD8+ lymphocytes and a peak increase of CK at 72 h. IL-6 and CRP increased only after concentric exercise within the first 24 h. Both exercises resulted in a decrease of monocyte activation (LFA-1: CD18+/CD11a+) after 6 h, with an increase for the eccentric exercise part after 24 h (P < 0.05).

Conclusion: Accustomed concentric exercise mainly induced an acute-phase response, with increased CRP, IL-6, and activation of CD4+ lymphocyte subsets. Unaccustomed eccentric exercise provided a delayed increase in CK and activation of monocytes and CD4+ and CD8+ subsets. Therefore, the immunological reaction depends not only on the type of contraction but also on the adaptation to the exercise.

1Department of Pneumology, University Hospital Freiburg, GERMANY; 2Department of Pneumology, University Hospital Rostock, GERMANY; 3Department of Sports Science, Technical University of Munich, GERMANY; and 4Department of Preventive and Rehabilitative Sports Medicine, University Hospital Freiburg, GERMANY

Address for correspondence: Stephan Sorichter, M.D., Department of Pneumology, University Hospital Freiburg, Killianstr. 5, D-79106 Freiburg, Germany; E-mail: sorichter@med1.ukl.uni-freiburg.de.

Submitted for publication December 2005.

Accepted for publication May 2006.

©2006The American College of Sports Medicine