The relation of daily energy expenditure (EE) and maximal oxygen uptake(˙VO2max) to plasma fibrinogen with reference to DNA polymorphism was analyzed in a random sample of men (N = 189), age 50-60. Fibrinogen polymorphism was detected using polymerase chain reaction (PCR) and digestion with HindIII and BclI (β-fibrinogen), and TaqI(α-fibrinogen) restriction enzymes. Mean ˙VO2max was 29.4 ml·kg-1·min-1 (95%CI 28.5; 30.2) and mean daily EE was 179 kJ·kg-1·d-1 (173; 186) and were similar in all fibrinogen genotypes. Plasma fibrinogen was 3.26 g·l-1(3.18; 3.34) and did not associate with fibrinogen polymorphisms. Both EE and˙VO2max related inversely to fibrinogen level (r = -0.24,P < 0.001). Strongest predictors of plasma fibrinogen were˙VO2max in TaqI 800 bp homozygotes, and EE together with smoking in TaqI 900 bp homozygotes. The predictive role of ˙VO2max was marginal in the common β-fibrinogen genotypes, whereas physical activity level explained up to 9% of the variance in the less frequent genotypes. These data suggest that the association of ˙VO2max and EE with plasma fibrinogen varies across the fibrinogen genotypes.
Kuopio Research Institute of Exercise Medicine and Department of Physiology, University of Kuopio, Puistokatu 20, FIN-70110, Kuopio, FINLAND; and Physical Activity Sciences Laboratory, Laval University, Ste-Foy, Québec, CANADA
Submitted for publication February 1995.
Accepted for publication August 1995.
The authors wish to acknowledge the expert technical help of Monique Chagnon and Manon Belair.
This study was supported by grants from the Ministry of Education in Finland (108/722/91; 131/722/91; 133/722/91; 135/722/91; 138/722/91; 247/722/92; 249/722/92), from the City of Kuopio, and from the Centre de Recherche et d'Information Nutritionelles de Paris (CERIN).
Dr. Rainer Rauramaa is currently a fellow from FRSQ (Fonds de la Recherche en Sante du Québec).
Address for correspondence: Dr. Rainer Rauramaa, Kuopio Research Institute of Exercise Medicine, Puistokatu 20, FIN-70110, Kuopio, Finland; E-mail:Rainer.Rauramaa@messi.uku.fi